CN102712912A - 在乳酸菌中改善流向乙酰乳酸来源产物的流量 - Google Patents

在乳酸菌中改善流向乙酰乳酸来源产物的流量 Download PDF

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CN102712912A
CN102712912A CN2010800539407A CN201080053940A CN102712912A CN 102712912 A CN102712912 A CN 102712912A CN 2010800539407 A CN2010800539407 A CN 2010800539407A CN 201080053940 A CN201080053940 A CN 201080053940A CN 102712912 A CN102712912 A CN 102712912A
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seq
gene
lactic
cell
acid bacteria
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B·J·保罗
W·苏
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Butamax Advanced Biofuels LLC
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2010800539407A 2009-09-29 2010-09-29 在乳酸菌中改善流向乙酰乳酸来源产物的流量 Pending CN102712912A (zh)

Applications Claiming Priority (3)

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US24671709P 2009-09-29 2009-09-29
US61/246717 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

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CN102712912A true CN102712912A (zh) 2012-10-03

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US (1) US9169499B2 (enExample)
EP (1) EP2483400A1 (enExample)
JP (1) JP2013505739A (enExample)
CN (1) CN102712912A (enExample)
AU (1) AU2010300722A1 (enExample)
BR (1) BR112012006941A2 (enExample)
CA (1) CA2776151A1 (enExample)
IN (1) IN2012DN02396A (enExample)
WO (1) WO2011041402A1 (enExample)

Cited By (9)

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Publication number Priority date Publication date Assignee Title
CN107922917A (zh) * 2015-06-12 2018-04-17 Cj第制糖株式会社 乳酸生产率提高的微生物和使用其生产乳酸的方法
CN108368523A (zh) * 2015-10-09 2018-08-03 丹麦技术大学 在代谢工程化的乳酸菌中高水平生产双乙酰
CN108486176A (zh) * 2018-05-18 2018-09-04 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN108570438A (zh) * 2017-03-08 2018-09-25 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN110431229A (zh) * 2016-12-14 2019-11-08 瓦赫宁根大学 热稳定的Cas9核酸酶
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN112601809A (zh) * 2018-08-03 2021-04-02 米兰-比科卡大学 细胞生物合成聚d-乳酸和聚l-乳酸的方法
CN113473998A (zh) * 2019-02-01 2021-10-01 农业食品和环境国家研究中心 用于预防和/或治疗内脏痛的乳酸乳球菌菌株
CN114457122A (zh) * 2020-05-13 2022-05-10 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

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WO2011041426A1 (en) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved yeast production host cells
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CN102762722B (zh) 2009-12-29 2015-05-20 布特马斯先进生物燃料有限责任公司 用于发酵生产低级烷基醇的醇脱氢酶(adh)
US9297016B2 (en) 2010-02-17 2016-03-29 Butamax Advanced Biofuels Llc Activity of Fe—S cluster requiring proteins
EP3269806B1 (en) 2010-09-07 2020-05-27 Butamax (TM) Advanced Biofuels LLC Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
CA2831130C (en) 2011-03-24 2021-10-26 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
JP2014519811A (ja) 2011-04-29 2014-08-21 ダニスコ・ユーエス・インク チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産
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CN102559736B (zh) * 2012-02-24 2014-01-08 常茂生物化学工程股份有限公司 提高富马酸产量的基因工程菌株及构建方法及其应用
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WO2013176909A2 (en) 2012-05-11 2013-11-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
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CN107922917A (zh) * 2015-06-12 2018-04-17 Cj第制糖株式会社 乳酸生产率提高的微生物和使用其生产乳酸的方法
CN108368523A (zh) * 2015-10-09 2018-08-03 丹麦技术大学 在代谢工程化的乳酸菌中高水平生产双乙酰
CN110431229B (zh) * 2016-12-14 2024-03-12 瓦赫宁根大学 热稳定的Cas9核酸酶
CN110431229A (zh) * 2016-12-14 2019-11-08 瓦赫宁根大学 热稳定的Cas9核酸酶
US12071639B2 (en) 2016-12-14 2024-08-27 Wageningen Universiteit Thermostable Cas9 nucleases
US11976306B2 (en) 2016-12-14 2024-05-07 Stichting Voor De Technische Wetenschappen Thermostable CAS9 nucleases
US11939605B2 (en) 2016-12-14 2024-03-26 Wageningen Universiteit Thermostable CAS9 nucleases
CN108570438A (zh) * 2017-03-08 2018-09-25 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN108486176A (zh) * 2018-05-18 2018-09-04 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN108486176B (zh) * 2018-05-18 2021-08-03 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN112601809A (zh) * 2018-08-03 2021-04-02 米兰-比科卡大学 细胞生物合成聚d-乳酸和聚l-乳酸的方法
CN113473998A (zh) * 2019-02-01 2021-10-01 农业食品和环境国家研究中心 用于预防和/或治疗内脏痛的乳酸乳球菌菌株
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN114457122B (zh) * 2020-05-13 2023-06-20 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用
CN114457122A (zh) * 2020-05-13 2022-05-10 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

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US20110136192A1 (en) 2011-06-09
BR112012006941A2 (pt) 2015-09-08
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EP2483400A1 (en) 2012-08-08
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