BR112012006941A2 - célula bacteriana de ácido recombinante, método de produção de uma célula bacteriana do ácido lético recombinamte, método de produção de isobutanol, vetor de integração de bastérias do ácido lático e método para integrar aleatoriamente um segmento de dna em uma célula bacteriana do ácido lético genoma - Google Patents

célula bacteriana de ácido recombinante, método de produção de uma célula bacteriana do ácido lético recombinamte, método de produção de isobutanol, vetor de integração de bastérias do ácido lático e método para integrar aleatoriamente um segmento de dna em uma célula bacteriana do ácido lético genoma

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Publication number
BR112012006941A2
BR112012006941A2 BR112012006941A BR112012006941A BR112012006941A2 BR 112012006941 A2 BR112012006941 A2 BR 112012006941A2 BR 112012006941 A BR112012006941 A BR 112012006941A BR 112012006941 A BR112012006941 A BR 112012006941A BR 112012006941 A2 BR112012006941 A2 BR 112012006941A2
Authority
BR
Brazil
Prior art keywords
bacterial cell
lactic acid
acid bacterial
recombinamte
boerase
Prior art date
Application number
BR112012006941A
Other languages
English (en)
Portuguese (pt)
Inventor
Brian James Paul
Wonchul Suh
Original Assignee
Butamax Tm Advanced Biofuels
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Tm Advanced Biofuels filed Critical Butamax Tm Advanced Biofuels
Publication of BR112012006941A2 publication Critical patent/BR112012006941A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
BR112012006941A 2009-09-29 2010-09-29 célula bacteriana de ácido recombinante, método de produção de uma célula bacteriana do ácido lético recombinamte, método de produção de isobutanol, vetor de integração de bastérias do ácido lático e método para integrar aleatoriamente um segmento de dna em uma célula bacteriana do ácido lético genoma BR112012006941A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24671709P 2009-09-29 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

Publications (1)

Publication Number Publication Date
BR112012006941A2 true BR112012006941A2 (pt) 2015-09-08

Family

ID=43093547

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012006941A BR112012006941A2 (pt) 2009-09-29 2010-09-29 célula bacteriana de ácido recombinante, método de produção de uma célula bacteriana do ácido lético recombinamte, método de produção de isobutanol, vetor de integração de bastérias do ácido lático e método para integrar aleatoriamente um segmento de dna em uma célula bacteriana do ácido lético genoma

Country Status (9)

Country Link
US (1) US9169499B2 (enExample)
EP (1) EP2483400A1 (enExample)
JP (1) JP2013505739A (enExample)
CN (1) CN102712912A (enExample)
AU (1) AU2010300722A1 (enExample)
BR (1) BR112012006941A2 (enExample)
CA (1) CA2776151A1 (enExample)
IN (1) IN2012DN02396A (enExample)
WO (1) WO2011041402A1 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
CA2678261A1 (en) 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
KR101573775B1 (ko) 2007-04-18 2015-12-04 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 고도 활성 케톨-산 리덕토아이소머라아제 효소를 이용한 아이소부탄올의 발효 생산
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
KR20110063576A (ko) 2008-09-29 2011-06-10 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 효모에서 증가된 이종성 Ｆｅ-Ｓ 효소 활성
WO2011041426A1 (en) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved yeast production host cells
KR20120099509A (ko) 2009-12-29 2012-09-10 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 재조합 숙주 세포에서 육탄당 키나아제의 발현
CN102762722B (zh) 2009-12-29 2015-05-20 布特马斯先进生物燃料有限责任公司 用于发酵生产低级烷基醇的醇脱氢酶(adh)
US9297016B2 (en) 2010-02-17 2016-03-29 Butamax Advanced Biofuels Llc Activity of Fe—S cluster requiring proteins
EP3269806B1 (en) 2010-09-07 2020-05-27 Butamax (TM) Advanced Biofuels LLC Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
CA2831130C (en) 2011-03-24 2021-10-26 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
JP2014519811A (ja) 2011-04-29 2014-08-21 ダニスコ・ユーエス・インク チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産
JP6093764B2 (ja) 2011-07-28 2017-03-08 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー ケトイソ吉草酸デカルボキシラーゼ酵素およびその使用方法
CA2861613A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Fermentative production of alcohols
CN102559736B (zh) * 2012-02-24 2014-01-08 常茂生物化学工程股份有限公司 提高富马酸产量的基因工程菌株及构建方法及其应用
CN104284981A (zh) 2012-03-23 2015-01-14 布特马斯先进生物燃料有限责任公司 在针对产丁醇生物的培养基中补充乙酸盐
WO2013176909A2 (en) 2012-05-11 2013-11-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
CN102816787B (zh) * 2012-06-20 2015-05-06 江南大学 利用重组大肠杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
WO2014025604A2 (en) * 2012-08-07 2014-02-13 Gevo, Inc. Microorganisms for improved production of fuels, chemicals, and amino acids
BR112015003701A2 (pt) 2012-08-22 2017-12-12 Butamax Advanced Biofuels Llc células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição
CN102876704A (zh) * 2012-09-20 2013-01-16 江南大学 利用重组枯草芽孢杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
US9512408B2 (en) 2012-09-26 2016-12-06 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
WO2014160050A1 (en) 2013-03-14 2014-10-02 Butamax Advanced Biofuels Llc Glycerol 3- phosphate dehydrogenase for butanol production
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
US20150122558A1 (en) 2013-09-09 2015-05-07 Columbia Trailer Co., Inc. Lifting jack assembly with rotatable hydraulic cylinder for steering and method of operation
JP6499587B2 (ja) * 2013-11-22 2019-04-10 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
CA2957920A1 (en) 2014-08-11 2016-02-18 Butamax Advanced Biofuels Llc Yeast preparations and methods of making the same
US10155967B2 (en) * 2015-06-12 2018-12-18 Cj Cheiljedang Corporation Microorganism having enhanced productivity of lactic acid and a process for producing lactic acid using the same
EP3359678A1 (en) * 2015-10-09 2018-08-15 Danmarks Tekniske Universitet High-level production of diacetyl in a metabolically engineered lactic acid bacterium
CA3046824A1 (en) 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
CN108570438B (zh) * 2017-03-08 2020-06-30 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN108486176B (zh) * 2018-05-18 2021-08-03 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
IT201800007846A1 (it) * 2018-08-03 2020-02-03 Università Degli Studi Di Milano - Bicocca Procedimento per la biosintesi cellulare di acido poli d-lattico e di acido poli l-lattico
CA3127726A1 (en) * 2019-02-01 2020-08-06 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Lactococcus lactis strains for the prevention and/or the treatment of visceral pain
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN114457122B (zh) * 2020-05-13 2023-06-20 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0928333B2 (en) * 1996-08-22 2009-10-07 Chr. Hansen A/S Metabolically engineered lactic acid bacteria and their use
FR2777905B1 (fr) 1998-04-23 2002-07-19 Agronomique Inst Nat Rech Bacteries surproductrices d'alpha-acetolactate, et procede d'obtention
DE19855312A1 (de) 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
WO2004105518A1 (en) 2003-05-22 2004-12-09 Hilltop Ranch, Inc. Apparatus and process for almond pasteurization
TWI348262B (en) 2005-02-10 2011-09-01 Bruno Ferrario A circuit and method for adaptive frequency compensation for dc-to-dc converter
JP5149785B2 (ja) 2005-04-12 2013-02-20 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー エタノールを得るためのバイオマスの処理
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
NZ566406A (en) 2005-10-26 2012-04-27 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
KR101573775B1 (ko) 2007-04-18 2015-12-04 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 고도 활성 케톨-산 리덕토아이소머라아제 효소를 이용한 아이소부탄올의 발효 생산
US8130756B2 (en) 2007-07-13 2012-03-06 Hewlett-Packard Development Company, L.P. Tunnel configuration associated with packet checking in a network
EP2677028B1 (en) 2007-12-20 2016-03-09 Butamax (TM) Advanced Biofuels LLC Ketol-acid reductoisomerase using NADH
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US8828695B2 (en) 2008-06-04 2014-09-09 Butamax Advanced Biofuels Llc Method for producing butanol using two-phase extractive fermentation
BRPI0909989A2 (pt) 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
WO2010037105A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
CA2735948A1 (en) 2008-09-29 2011-04-01 Butamaxtm Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
BRPI0913680A2 (pt) 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-láctica, método para aumentar a atividade de um polipeptídeo dihidroxi-ácido desidratase heterólogo em uma célula bacteriana ácido-láctica e método de produção de insobutanol"
BRPI0914521A2 (pt) 2008-10-27 2016-07-26 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante, método de aumento da produção de isobutanol e método de produção de isobutanol
US20110195505A1 (en) 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
PL2957322T3 (pl) 2014-06-18 2020-05-18 Fotona D.O.O. Laserowa głowica zabiegowa i system laserowy
US10078608B2 (en) 2016-09-16 2018-09-18 Intel Corporation USB-C multiple connector support for host and device mode configurations

Also Published As

Publication number Publication date
JP2013505739A (ja) 2013-02-21
CN102712912A (zh) 2012-10-03
US9169499B2 (en) 2015-10-27
IN2012DN02396A (enExample) 2015-08-21
AU2010300722A1 (en) 2012-04-12
US20110136192A1 (en) 2011-06-09
WO2011041402A1 (en) 2011-04-07
EP2483400A1 (en) 2012-08-08
CA2776151A1 (en) 2011-04-07

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