JP2013503877A5 - - Google Patents
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- Publication number
- JP2013503877A5 JP2013503877A5 JP2012527990A JP2012527990A JP2013503877A5 JP 2013503877 A5 JP2013503877 A5 JP 2013503877A5 JP 2012527990 A JP2012527990 A JP 2012527990A JP 2012527990 A JP2012527990 A JP 2012527990A JP 2013503877 A5 JP2013503877 A5 JP 2013503877A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- buffer
- low
- elution
- separated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 26
- 229920001184 polypeptide Polymers 0.000 claims 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims 20
- 239000006174 pH buffer Substances 0.000 claims 11
- 239000012149 elution buffer Substances 0.000 claims 6
- 239000012560 cell impurity Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 3
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims 3
- 239000000178 monomer Substances 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23886709P | 2009-09-01 | 2009-09-01 | |
| US61/238,867 | 2009-09-01 | ||
| US25343809P | 2009-10-20 | 2009-10-20 | |
| US61/253,438 | 2009-10-20 | ||
| PCT/US2010/047448 WO2011028753A1 (en) | 2009-09-01 | 2010-09-01 | Enhanced protein purification through a modified protein a elution |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013503877A JP2013503877A (ja) | 2013-02-04 |
| JP2013503877A5 true JP2013503877A5 (enExample) | 2013-10-10 |
| JP5787891B2 JP5787891B2 (ja) | 2015-09-30 |
Family
ID=43649614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012527990A Active JP5787891B2 (ja) | 2009-09-01 | 2010-09-01 | 改変されたプロテインａ溶離による向上したタンパク質精製 |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US9428548B2 (enExample) |
| EP (2) | EP2473617B1 (enExample) |
| JP (1) | JP5787891B2 (enExample) |
| KR (1) | KR101764449B1 (enExample) |
| CN (2) | CN102686738A (enExample) |
| BR (1) | BR112012004697B8 (enExample) |
| CA (1) | CA2772653C (enExample) |
| ES (1) | ES2787234T3 (enExample) |
| HR (1) | HRP20200581T1 (enExample) |
| MX (1) | MX2012002565A (enExample) |
| PL (1) | PL2473617T3 (enExample) |
| RU (1) | RU2571929C2 (enExample) |
| SI (1) | SI2473617T1 (enExample) |
| WO (1) | WO2011028753A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| CN103276033A (zh) | 2006-09-13 | 2013-09-04 | 雅培制药有限公司 | 细胞培养改良 |
| CN105111309A (zh) | 2008-10-20 | 2015-12-02 | Abbvie公司 | 使用a蛋白亲和层析分离和纯化抗体 |
| CN104974251A (zh) | 2008-10-20 | 2015-10-14 | Abbvie公司 | 在抗体纯化过程中的病毒灭活 |
| PT2462158T (pt) | 2009-08-06 | 2018-02-23 | H Hoffnabb La Roche Ag | Método para melhorar a remoção de vírus na purificação de proteínas |
| ES2526876T3 (es) * | 2009-09-10 | 2015-01-16 | Lonza Biologics Plc. | Método y sistema para purificación de polipéptidos |
| HRP20181846T1 (hr) * | 2011-12-22 | 2018-12-28 | Genentech, Inc. | Kromatografija membranom za ionsku izmjenu |
| WO2013147691A1 (en) * | 2012-03-28 | 2013-10-03 | Ge Healthcare Bio-Sciences Ab | Affinity chromatography matrix |
| SG11201406407TA (en) | 2012-06-29 | 2014-11-27 | Emd Millipore Corp | Methods for inactivating viruses during a protein purification process |
| US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| KR101838039B1 (ko) * | 2013-03-14 | 2018-03-14 | 이엠디 밀리포어 코포레이션 | 단백질 a 기반 크로마토그래피를 이용한 단백질 순도의 증가 방법 |
| CN105377873B (zh) * | 2013-07-12 | 2021-03-30 | 豪夫迈·罗氏有限公司 | 阐明离子交换层析输入优化 |
| MX380902B (es) * | 2013-11-15 | 2025-03-12 | Genentech Inc | Métodos para la inactivación viral usando detergentes ecológico. |
| EP3674310A1 (en) * | 2014-03-10 | 2020-07-01 | Richter Gedeon Nyrt. | Immunoglobulin purification using pre-cleaning steps |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| TWI848953B (zh) | 2018-06-09 | 2024-07-21 | 德商百靈佳殷格翰國際股份有限公司 | 針對癌症治療之多特異性結合蛋白 |
| CN112566930A (zh) * | 2018-06-19 | 2021-03-26 | 百时美施贵宝公司 | 使用色谱纯化蛋白质的方法 |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CA3167669A1 (en) * | 2020-01-17 | 2021-07-22 | Regeneron Pharmaceuticals, Inc. | Hydrophobic interaction chromatography for viral clearance |
| KR20250011797A (ko) | 2023-07-13 | 2025-01-22 | 주식회사 녹십자 | 알부민이 융합된 단백질의 정제방법 |
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-
2010
- 2010-09-01 CN CN2010800389895A patent/CN102686738A/zh active Pending
- 2010-09-01 EP EP10814394.2A patent/EP2473617B1/en not_active Revoked
- 2010-09-01 PL PL10814394T patent/PL2473617T3/pl unknown
- 2010-09-01 JP JP2012527990A patent/JP5787891B2/ja active Active
- 2010-09-01 CA CA2772653A patent/CA2772653C/en active Active
- 2010-09-01 KR KR1020127008252A patent/KR101764449B1/ko active Active
- 2010-09-01 EP EP20159179.9A patent/EP3736338A1/en not_active Withdrawn
- 2010-09-01 WO PCT/US2010/047448 patent/WO2011028753A1/en not_active Ceased
- 2010-09-01 US US13/393,525 patent/US9428548B2/en active Active
- 2010-09-01 HR HRP20200581TT patent/HRP20200581T1/hr unknown
- 2010-09-01 SI SI201032003T patent/SI2473617T1/sl unknown
- 2010-09-01 ES ES10814394T patent/ES2787234T3/es active Active
- 2010-09-01 BR BR112012004697A patent/BR112012004697B8/pt active IP Right Grant
- 2010-09-01 CN CN201810100462.9A patent/CN108409829A/zh active Pending
- 2010-09-01 MX MX2012002565A patent/MX2012002565A/es active IP Right Grant
- 2010-09-01 RU RU2012112536/10A patent/RU2571929C2/ru active
-
2021
- 2021-05-27 US US17/332,907 patent/US20220081466A1/en not_active Abandoned
- 2021-12-21 US US17/558,406 patent/US20220324905A1/en not_active Abandoned
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