KR101764449B1 - 변형된 단백질 ａ 용리를 통한 증진된 단백질 정제 - Google Patents

변형된 단백질 ａ 용리를 통한 증진된 단백질 정제 Download PDF

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KR101764449B1
KR101764449B1 KR1020127008252A KR20127008252A KR101764449B1 KR 101764449 B1 KR101764449 B1 KR 101764449B1 KR 1020127008252 A KR1020127008252 A KR 1020127008252A KR 20127008252 A KR20127008252 A KR 20127008252A KR 101764449 B1 KR101764449 B1 KR 101764449B1
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polypeptide
protein
delete delete
antibody
buffer
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KR20120106719A (ko
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아릭 브라운
크리스토퍼 제이. 다우드
아샤 난디니 라다모한
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제넨테크, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020127008252A 2009-09-01 2010-09-01 변형된 단백질 ａ 용리를 통한 증진된 단백질 정제 Active KR101764449B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US23886709P 2009-09-01 2009-09-01
US61/238,867 2009-09-01
US25343809P 2009-10-20 2009-10-20
US61/253,438 2009-10-20
PCT/US2010/047448 WO2011028753A1 (en) 2009-09-01 2010-09-01 Enhanced protein purification through a modified protein a elution

Publications (2)

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KR20120106719A KR20120106719A (ko) 2012-09-26
KR101764449B1 true KR101764449B1 (ko) 2017-08-02

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US (3) US9428548B2 (enExample)
EP (2) EP2473617B1 (enExample)
JP (1) JP5787891B2 (enExample)
KR (1) KR101764449B1 (enExample)
CN (2) CN102686738A (enExample)
BR (1) BR112012004697B8 (enExample)
CA (1) CA2772653C (enExample)
ES (1) ES2787234T3 (enExample)
HR (1) HRP20200581T1 (enExample)
MX (1) MX2012002565A (enExample)
PL (1) PL2473617T3 (enExample)
RU (1) RU2571929C2 (enExample)
SI (1) SI2473617T1 (enExample)
WO (1) WO2011028753A1 (enExample)

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KR20250011797A (ko) 2023-07-13 2025-01-22 주식회사 녹십자 알부민이 융합된 단백질의 정제방법

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NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
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KR20120065383A (ko) * 2009-09-10 2012-06-20 론자 바이올로직스 피엘씨 폴리펩티드 정제를 위한 방법 및 시스템
HRP20181846T1 (hr) * 2011-12-22 2018-12-28 Genentech, Inc. Kromatografija membranom za ionsku izmjenu
JP6420756B2 (ja) * 2012-03-28 2018-11-07 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ アフィニティークロマトグラフィーマトリックス
JP6182210B2 (ja) 2012-06-29 2017-08-16 イー・エム・デイー・ミリポア・コーポレイシヨン タンパク質精製プロセス中のウイルスの不活性化方法
US8921113B2 (en) 2012-12-21 2014-12-30 Dionex Corporation Buffer kit and method of generating a linear pH gradient
ES2735354T3 (es) 2013-03-14 2019-12-18 Emd Millipore Corp Métodos para aumentar la pureza de proteínas utilizando la cromatografía basada en proteína A
RU2016104542A (ru) * 2013-07-12 2017-08-17 Дженентек, Инк. Осуществление оптимизации входных условий ионообменной хроматографии
PL3068417T3 (pl) * 2013-11-15 2024-05-13 F.Hoffmann-La Roche Ag Sposoby inaktywacji wirusów przy użyciu ekologicznych detergentów
DK3116891T3 (da) * 2014-03-10 2020-05-04 Richter Gedeon Nyrt Immunglobulinrensning ved hjælp af forrensningstrin
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
TWI848953B (zh) 2018-06-09 2024-07-21 德商百靈佳殷格翰國際股份有限公司 針對癌症治療之多特異性結合蛋白
CN112566930A (zh) * 2018-06-19 2021-03-26 百时美施贵宝公司 使用色谱纯化蛋白质的方法
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250011797A (ko) 2023-07-13 2025-01-22 주식회사 녹십자 알부민이 융합된 단백질의 정제방법

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US20220324905A1 (en) 2022-10-13
EP2473617A4 (en) 2014-04-09
ES2787234T3 (es) 2020-10-15
JP5787891B2 (ja) 2015-09-30
CN108409829A (zh) 2018-08-17
CN102686738A (zh) 2012-09-19
US20130041139A1 (en) 2013-02-14
BR112012004697B1 (pt) 2021-02-09
BR112012004697A2 (pt) 2015-09-08
CA2772653A1 (en) 2011-03-10
MX2012002565A (es) 2012-05-29
RU2012112536A (ru) 2013-10-10
RU2571929C2 (ru) 2015-12-27
EP2473617A1 (en) 2012-07-11
CA2772653C (en) 2019-06-25
JP2013503877A (ja) 2013-02-04
SI2473617T1 (sl) 2020-07-31
PL2473617T3 (pl) 2020-06-29
HRP20200581T1 (hr) 2020-07-10
EP2473617B1 (en) 2020-02-26
WO2011028753A1 (en) 2011-03-10
US20220081466A1 (en) 2022-03-17
US9428548B2 (en) 2016-08-30
EP3736338A1 (en) 2020-11-11
BR112012004697B8 (pt) 2021-05-25
KR20120106719A (ko) 2012-09-26

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