JP2012530782A5 - - Google Patents
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- Publication number
- JP2012530782A5 JP2012530782A5 JP2012517634A JP2012517634A JP2012530782A5 JP 2012530782 A5 JP2012530782 A5 JP 2012530782A5 JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012530782 A5 JP2012530782 A5 JP 2012530782A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- group
- thiol
- refolding
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- 235000018102 proteins Nutrition 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 150000003573 thiols Chemical class 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 239000011159 matrix material Substances 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 3
- 238000002955 isolation Methods 0.000 claims 3
- 239000004475 Arginine Substances 0.000 claims 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 239000007983 Tris buffer Substances 0.000 claims 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 2
- 238000004220 aggregation Methods 0.000 claims 2
- 230000002776 aggregation Effects 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 239000002563 ionic surfactant Substances 0.000 claims 2
- 239000002736 nonionic surfactant Substances 0.000 claims 2
- 230000000065 osmolyte Effects 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 2
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 2
- 235000011151 potassium sulphates Nutrition 0.000 claims 2
- 229940124272 protein stabilizer Drugs 0.000 claims 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 2
- 235000011152 sodium sulphate Nutrition 0.000 claims 2
- 239000000600 sorbitol Substances 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 150000005846 sugar alcohols Polymers 0.000 claims 2
- RYECOJGRJDOGPP-UHFFFAOYSA-N Ethylurea Chemical compound CCNC(N)=O RYECOJGRJDOGPP-UHFFFAOYSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 108010053070 Glutathione Disulfide Proteins 0.000 claims 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- MGJKQDOBUOMPEZ-UHFFFAOYSA-N N,N'-dimethylurea Chemical compound CNC(=O)NC MGJKQDOBUOMPEZ-UHFFFAOYSA-N 0.000 claims 1
- XGEGHDBEHXKFPX-UHFFFAOYSA-N N-methylthiourea Natural products CNC(N)=O XGEGHDBEHXKFPX-UHFFFAOYSA-N 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 claims 1
- 229940099500 cystamine Drugs 0.000 claims 1
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- 238000011118 depth filtration Methods 0.000 claims 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 claims 1
- 210000003000 inclusion body Anatomy 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000005342 ion exchange Methods 0.000 claims 1
- 229960003151 mercaptamine Drugs 0.000 claims 1
- XGEGHDBEHXKFPX-NJFSPNSNSA-N methylurea Chemical compound [14CH3]NC(N)=O XGEGHDBEHXKFPX-NJFSPNSNSA-N 0.000 claims 1
- YPZRWBKMTBYPTK-UHFFFAOYSA-N oxidized gamma-L-glutamyl-L-cysteinylglycine Natural products OC(=O)C(N)CCC(=O)NC(C(=O)NCC(O)=O)CSSCC(C(=O)NCC(O)=O)NC(=O)CCC(N)C(O)=O YPZRWBKMTBYPTK-UHFFFAOYSA-N 0.000 claims 1
- 239000012562 protein A resin Substances 0.000 claims 1
- 239000006176 redox buffer Substances 0.000 claims 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21925709P | 2009-06-22 | 2009-06-22 | |
| US61/219,257 | 2009-06-22 | ||
| PCT/US2010/039390 WO2011005488A1 (en) | 2009-06-22 | 2010-06-21 | Refolding proteins using a chemically controlled redox state |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015175422A Division JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012530782A JP2012530782A (ja) | 2012-12-06 |
| JP2012530782A5 true JP2012530782A5 (https=) | 2013-07-18 |
| JP5808323B2 JP5808323B2 (ja) | 2015-11-10 |
Family
ID=42710673
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012517634A Active JP5808323B2 (ja) | 2009-06-22 | 2010-06-21 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
| JP2015175422A Active JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015175422A Active JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Country Status (15)
| Country | Link |
|---|---|
| US (5) | US8952138B2 (https=) |
| EP (2) | EP3366692A1 (https=) |
| JP (2) | JP5808323B2 (https=) |
| KR (1) | KR101741859B1 (https=) |
| CN (1) | CN102482321B (https=) |
| AU (1) | AU2010270986B2 (https=) |
| BR (1) | BRPI1011940B8 (https=) |
| CA (1) | CA2765881C (https=) |
| CL (1) | CL2011003278A1 (https=) |
| EA (1) | EA020621B1 (https=) |
| IL (1) | IL216954A (https=) |
| MX (1) | MX2011013898A (https=) |
| SG (1) | SG176963A1 (https=) |
| WO (1) | WO2011005488A1 (https=) |
| ZA (1) | ZA201200512B (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3366692A1 (en) | 2009-06-22 | 2018-08-29 | Amgen, Inc | Refolding proteins using a chemically controlled redox state |
| AU2010266093B2 (en) | 2009-06-25 | 2013-06-27 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| EP2417982A1 (en) * | 2010-07-30 | 2012-02-15 | Ferring B.V. | Stabilization of gonadotropins |
| US20150376228A1 (en) * | 2013-02-22 | 2015-12-31 | Biogenomics Limited | Process for high efficiency refolding of recombinant proteins |
| WO2017019776A1 (en) * | 2015-07-27 | 2017-02-02 | Purdue Research Foundation | Tandem folding methods to improve protein folding yield |
| EP3344651B1 (en) | 2015-09-02 | 2022-03-02 | Merck Sharp & Dohme Corp. | A process for obtaining insulin with correctly formed disulfide bonds |
| KR102579850B1 (ko) * | 2016-07-22 | 2023-09-18 | 암젠 인크 | Fc-함유 단백질의 정제 방법 |
| CN108276470A (zh) * | 2017-01-06 | 2018-07-13 | 深圳市新产业生物医学工程股份有限公司 | 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒 |
| CN110494120B (zh) * | 2017-04-10 | 2023-07-18 | 花王株式会社 | 去除角栓的方法 |
| WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
| EP4083058A3 (en) * | 2018-11-07 | 2023-01-11 | Applied Molecular Transport Inc. | Delivery constructs for transcytosis and related methods |
| ES3040791T3 (en) | 2020-12-18 | 2025-11-05 | Richter Gedeon Nyrt | Methods for the purification of refolded fc-peptide fusion protein |
| CN115894604B (zh) * | 2022-12-16 | 2024-01-23 | 康日百奥生物科技(苏州)有限公司 | 重组蛋白澄清纯化方法 |
Family Cites Families (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4468464A (en) | 1974-11-04 | 1984-08-28 | The Board Of Trustees Of The Leland Stanford Junior University | Biologically functional molecular chimeras |
| US4237224A (en) | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
| EP0122080B1 (en) | 1983-03-25 | 1989-09-13 | Celltech Limited | A process for the production of a protein |
| US4468454A (en) | 1983-06-10 | 1984-08-28 | E. I. Du Pont De Nemours And Company | Antifoggant process |
| US4572798A (en) * | 1984-12-06 | 1986-02-25 | Cetus Corporation | Method for promoting disulfide bond formation in recombinant proteins |
| GB8508340D0 (en) | 1985-03-29 | 1985-05-09 | Creighton T E | Production of protein |
| US4810643A (en) | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
| IL86090A (en) | 1987-04-16 | 1993-03-15 | Cetus Oncology Corp | Production of purified, biologically active, bacterially produced recombinant human csf-1 |
| CA1339757C (en) | 1987-04-16 | 1998-03-17 | Robert F. Halenbeck | Production of purified biologically active, bacterially produced recombinant human csf-1 |
| CA1329119C (en) | 1988-03-29 | 1994-05-03 | Milton David Goldenberg | Cytotoxic therapy |
| GB8807673D0 (en) | 1988-03-31 | 1988-05-05 | Lingner & Fischer Gmbh | Novel article |
| KR0133561B1 (ko) | 1988-05-13 | 1998-04-21 | 스티븐 엠. 오드레 | 과립구 군체 자극인자의 정제방법 |
| GB8927546D0 (en) * | 1989-12-06 | 1990-02-07 | Ciba Geigy | Process for the production of biologically active tgf-beta |
| US5986070A (en) | 1990-04-06 | 1999-11-16 | Amgen Inc. | Production of biologically active NGF proteins |
| AU664021B2 (en) * | 1990-09-05 | 1995-11-02 | Natinco Nv | Solubilization of proteins in active forms |
| WO1993018136A1 (en) | 1992-03-05 | 1993-09-16 | Cytomed, Inc. | Process for supporting hematopoietic progenitor cells |
| US5663304A (en) | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
| EP0657466B1 (en) | 1993-11-10 | 1997-01-15 | Pfizer Inc. | A process for refolding of (pro-)chymosin, comprising recycling of urea |
| US5466377A (en) | 1994-01-19 | 1995-11-14 | Grandics; Peter | Chromatography media and their uses |
| IL113497A (en) | 1994-04-28 | 2001-01-11 | Amgen Inc | Method for controlling metallophosphate precipitation in high cell density fermentations |
| AU2603995A (en) * | 1994-05-25 | 1995-12-18 | University Of Nebraska Board Of Regents | Biologically active glycoprotein hormones produced in procaryotic cells |
| AU6028396A (en) | 1995-06-07 | 1996-12-30 | Amgen, Inc. | Ob protein compositions and method |
| AU729540B2 (en) | 1996-01-23 | 2001-02-01 | Ortho-Mcneil Pharmaceutical, Inc. | Methods for purification and use of erythropoietin binding protein |
| US5935824A (en) | 1996-01-31 | 1999-08-10 | Technologene, Inc. | Protein expression system |
| US20020052026A1 (en) | 1997-10-08 | 2002-05-02 | Steven M. Vicik | Methods of refolding proteins |
| US6180391B1 (en) | 1998-01-28 | 2001-01-30 | Amgen Inc. | Highly efficient controlled expression of exogenous genes in e. coli |
| US6653098B1 (en) * | 1998-02-23 | 2003-11-25 | G. D. Searle & Co. | Method of producing mouse and human endostatin |
| WO1999060120A2 (en) | 1998-05-19 | 1999-11-25 | Avidex Limited | Soluble t cell receptor |
| US6660843B1 (en) | 1998-10-23 | 2003-12-09 | Amgen Inc. | Modified peptides as therapeutic agents |
| ATE313561T1 (de) | 1999-07-21 | 2006-01-15 | Amgen Inc | Vgf polypeptide und verfahren zur behandlung von vgf-erkrankungen |
| US6808902B1 (en) | 1999-11-12 | 2004-10-26 | Amgen Inc. | Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules |
| IL152804A0 (en) | 2000-05-16 | 2003-06-24 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
| CA2421588C (en) | 2000-09-05 | 2010-01-26 | Amgen Inc. | Tnf receptor-like molecules and uses thereof |
| IL157446A0 (en) | 2001-02-23 | 2004-03-28 | Immunex Corp Immunex Corp | Increased recovery of active proteins |
| US6972327B1 (en) | 2001-05-08 | 2005-12-06 | Immunex Corporation | Regeneration of chromatography material |
| US7138370B2 (en) | 2001-10-11 | 2006-11-21 | Amgen Inc. | Specific binding agents of human angiopoietin-2 |
| ITMI20012345A1 (it) | 2001-11-08 | 2003-05-08 | Internat Ct For Genetic En Gin | Procedimento per la produzione di interferone alfa di grado terapeutico |
| SI21102A (sl) | 2001-12-19 | 2003-06-30 | LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. | Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika |
| AU2002345829A1 (en) | 2002-06-24 | 2004-01-06 | Dr. Reddy's Laboratories Ltd. | Process for preparing g-csf |
| US7067279B1 (en) | 2002-08-23 | 2006-06-27 | Immunex Corporation | Cell culture performance with betaine |
| BR0317538A (pt) | 2002-12-20 | 2005-11-29 | Amgen Inc | Agente ligante, sequência de polinucleotìdeo, vetor de expressão, célula hospedeira, composição farmacêutica, e, métodos de inibir a atividade de miostatina, de aumentar a massa muscular magra e a razão de massa muscular magra para gordura, de tratar uma doença de emaciação muscular e um distúrbio metabólico relacionado com miostatina em um indivìduo, de detectar e medir miostatina em uma amostra, e, de diagnosticar um distúrbio relacionado com miostatina em um indivìduo |
| US7083948B1 (en) | 2002-12-24 | 2006-08-01 | Immunex Corporation | Polypeptide purification reagents and methods for their use |
| EP1449848A1 (en) | 2003-02-20 | 2004-08-25 | GBF German Research Centre for Biotechnology | Method for the production of cystine-knot proteins |
| GB0304068D0 (en) | 2003-02-22 | 2003-03-26 | Avidex Ltd | Substances |
| US7427659B2 (en) | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| EP1711512B1 (en) | 2004-01-30 | 2012-01-11 | Amgen Inc. | Process for purifying proteins |
| AU2005222782B2 (en) | 2004-02-04 | 2010-06-17 | Ratiopharm Gmbh | Methods of refolding mammalian glycosyltransferases |
| US20050209441A1 (en) | 2004-03-22 | 2005-09-22 | Lile Jackson D | Process for promoting proper folding of human serum albumin using a human serum albumin ligand |
| CA2577690C (en) | 2004-08-19 | 2013-08-06 | Biogen Idec Ma Inc. | Refolding transforming growth factor beta family proteins |
| FR2874318B1 (fr) | 2004-08-19 | 2006-11-24 | Oreal | Utilisation en cosmetique de composes polysaccharidiques amphoteres a chaine(s) polymerique(s) cationique(s) |
| DE102004041639A1 (de) | 2004-08-27 | 2006-03-02 | Bioceuticals Arzneimittel Ag | Verfahren zur Gewinnung von biologisch aktivem humanen G-CSF aus Inclusion Bodies |
| WO2006036834A2 (en) | 2004-09-24 | 2006-04-06 | Amgen Inc. | MODIFIED Fc MOLECULES |
| US7435804B2 (en) | 2004-10-19 | 2008-10-14 | Phage Biotechnology, Inc. | Method for obtaining single chain antibodies to human interferon α2b |
| AU2005299716B2 (en) | 2004-10-22 | 2012-09-06 | Amgen Inc. | Methods for refolding of recombinant antibodies |
| AU2005314438B2 (en) | 2004-12-10 | 2012-03-29 | Zymogenetics, Inc. | FGF18 production in prokaryotic hosts |
| JP2008528039A (ja) | 2005-01-28 | 2008-07-31 | ザイモジェネティクス，インコーポレイティド | Ｉｌ−３１の均質調製物 |
| WO2006097944A2 (en) | 2005-03-17 | 2006-09-21 | Zenotech Laboratories Limited | Process for the purification of recombinant granulocyte-colony stimulating factor |
| US7344962B2 (en) | 2005-06-21 | 2008-03-18 | International Business Machines Corporation | Method of manufacturing dual orientation wafers |
| DE102005033250A1 (de) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
| US8008453B2 (en) | 2005-08-12 | 2011-08-30 | Amgen Inc. | Modified Fc molecules |
| US7735525B2 (en) | 2005-11-14 | 2010-06-15 | Amgen Inc. | Thermally insulated apparatus for liquid chromatographic analysis |
| WO2007075283A2 (en) | 2005-12-06 | 2007-07-05 | Amgen Inc. | Polishing steps used in multi-step protein purification processes |
| BRPI0621124A2 (pt) | 2005-12-22 | 2011-11-29 | Genentech Inc | métodos para recuperação de uma proteìna de ligação à heparina |
| US7790862B2 (en) | 2006-06-13 | 2010-09-07 | Zymogenetics, Inc. | IL-17 and IL-23 antagonists and methods of using the same |
| US7612273B2 (en) * | 2006-03-20 | 2009-11-03 | Roland Corporation | Electronic percussion instrument |
| EP1845103B1 (en) * | 2006-04-10 | 2015-02-25 | Boehringer Ingelheim RCV GmbH & Co KG | Method for refolding a protein |
| AR062069A1 (es) | 2006-07-14 | 2008-10-15 | Genentech Inc | Replegado de proteinas recombinantes |
| EP2049821A4 (en) | 2006-07-21 | 2013-09-04 | Amgen Inc | Rupture valve |
| TW201307390A (zh) | 2007-02-02 | 2013-02-16 | Amgen Inc | 海帕西啶（ｈｅｐｃｉｄｉｎ）、海帕西啶拮抗劑及使用方法 |
| WO2008097829A2 (en) | 2007-02-02 | 2008-08-14 | Neose Technologies, Inc. | Large scale production of eukaryotic n-acetylglucosaminyltransferase i in bacteria |
| WO2008096370A2 (en) | 2007-02-05 | 2008-08-14 | Natco Pharma Limited | An efficient and novel purification method of recombinant hg-csf |
| WO2008100578A2 (en) | 2007-02-14 | 2008-08-21 | Amgen Inc. | Method of isolating antibodies by precipitation |
| JP2010536341A (ja) | 2007-08-15 | 2010-12-02 | アムニクス， インコーポレイテッド | 生物学的に活性なポリペプチドの特性を改変するための組成物および方法 |
| CN102015770A (zh) | 2008-02-29 | 2011-04-13 | 特拉维夫大学拉莫特有限公司 | 免疫球蛋白组合物和生产其的方法 |
| BRPI0914251B1 (pt) | 2008-06-25 | 2022-07-19 | Novartis Ag | Imunoligante recombinante, seu fragmento de ligação ao antígeno, uso dos mesmos, e composição |
| EP3366692A1 (en) | 2009-06-22 | 2018-08-29 | Amgen, Inc | Refolding proteins using a chemically controlled redox state |
| AU2010266093B2 (en) * | 2009-06-25 | 2013-06-27 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| TWI582408B (zh) | 2011-08-29 | 2017-05-11 | 安美基公司 | 用於非破壞性檢測－流體中未溶解粒子之方法及裝置 |
| US8945872B2 (en) | 2013-01-25 | 2015-02-03 | Warsaw Orthopedic, Inc. | Methods of purifying human recombinant growth and differentiation factor-5 (rhGDF-5) protein |
| US20160038588A1 (en) | 2013-03-15 | 2016-02-11 | Amgen Inc. | Myostatin Antagonism in Human Subjects |
| US9632095B2 (en) | 2014-12-01 | 2017-04-25 | University Of Delaware | Device and method for determining reaction kinetics |
| US9704239B1 (en) | 2016-09-02 | 2017-07-11 | Amgen Inc. | Video trigger synchronization for improved particle detection in a vessel |
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2010
- 2010-06-21 EP EP18167168.6A patent/EP3366692A1/en active Pending
- 2010-06-21 US US12/820,087 patent/US8952138B2/en active Active
- 2010-06-21 BR BRPI1011940A patent/BRPI1011940B8/pt active IP Right Grant
- 2010-06-21 KR KR1020127001716A patent/KR101741859B1/ko active Active
- 2010-06-21 AU AU2010270986A patent/AU2010270986B2/en active Active
- 2010-06-21 SG SG2011095411A patent/SG176963A1/en unknown
- 2010-06-21 CA CA2765881A patent/CA2765881C/en active Active
- 2010-06-21 WO PCT/US2010/039390 patent/WO2011005488A1/en not_active Ceased
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2017
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