CL2011003278A1 - Método para replegar una proteína expresada en un sistema de expresión no mamífero y presente en un volumen a una concentración de 2 g/l o mayor, que comprende poner en contacto la proteína con una solución amortiguadora al menos 2 mm que comprende un componente de óxidoreducción, desnaturalizante, antiagregante y estabilizador. - Google Patents
Método para replegar una proteína expresada en un sistema de expresión no mamífero y presente en un volumen a una concentración de 2 g/l o mayor, que comprende poner en contacto la proteína con una solución amortiguadora al menos 2 mm que comprende un componente de óxidoreducción, desnaturalizante, antiagregante y estabilizador.Info
- Publication number
- CL2011003278A1 CL2011003278A1 CL2011003278A CL2011003278A CL2011003278A1 CL 2011003278 A1 CL2011003278 A1 CL 2011003278A1 CL 2011003278 A CL2011003278 A CL 2011003278A CL 2011003278 A CL2011003278 A CL 2011003278A CL 2011003278 A1 CL2011003278 A1 CL 2011003278A1
- Authority
- CL
- Chile
- Prior art keywords
- protein
- refolding
- denaturing
- stabilizer
- concentration
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21925709P | 2009-06-22 | 2009-06-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2011003278A1 true CL2011003278A1 (es) | 2012-11-09 |
Family
ID=42710673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2011003278A CL2011003278A1 (es) | 2009-06-22 | 2011-12-22 | Método para replegar una proteína expresada en un sistema de expresión no mamífero y presente en un volumen a una concentración de 2 g/l o mayor, que comprende poner en contacto la proteína con una solución amortiguadora al menos 2 mm que comprende un componente de óxidoreducción, desnaturalizante, antiagregante y estabilizador. |
Country Status (15)
| Country | Link |
|---|---|
| US (5) | US8952138B2 (https=) |
| EP (2) | EP3366692A1 (https=) |
| JP (2) | JP5808323B2 (https=) |
| KR (1) | KR101741859B1 (https=) |
| CN (1) | CN102482321B (https=) |
| AU (1) | AU2010270986B2 (https=) |
| BR (1) | BRPI1011940B8 (https=) |
| CA (1) | CA2765881C (https=) |
| CL (1) | CL2011003278A1 (https=) |
| EA (1) | EA020621B1 (https=) |
| IL (1) | IL216954A (https=) |
| MX (1) | MX2011013898A (https=) |
| SG (1) | SG176963A1 (https=) |
| WO (1) | WO2011005488A1 (https=) |
| ZA (1) | ZA201200512B (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA020621B1 (ru) | 2009-06-22 | 2014-12-30 | Амген Инк. | Рефолдинг белков с использованием химически контролируемого окислительно-восстановительного состояния |
| LT3660032T (lt) | 2009-06-25 | 2026-02-25 | Amgen Inc. | Ne žinduolių sistemoje ekspresuotų baltymų surišamojo gryninimo būdai |
| EP2417982A1 (en) * | 2010-07-30 | 2012-02-15 | Ferring B.V. | Stabilization of gonadotropins |
| WO2014128726A2 (en) * | 2013-02-22 | 2014-08-28 | Biogenomics Limited | Process for high efficiency refolding of recombinant proteins |
| WO2017019776A1 (en) * | 2015-07-27 | 2017-02-02 | Purdue Research Foundation | Tandem folding methods to improve protein folding yield |
| US10745457B2 (en) | 2015-09-02 | 2020-08-18 | Merck Sharp & Dohme Corp. | Process for obtaining insulin with correctly formed disulfide bonds |
| CA3218290A1 (en) * | 2016-07-22 | 2018-01-25 | Amgen Inc. | Methods of purifying fc-containing proteins |
| CN108276470A (zh) * | 2017-01-06 | 2018-07-13 | 深圳市新产业生物医学工程股份有限公司 | 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒 |
| WO2018190301A1 (ja) * | 2017-04-10 | 2018-10-18 | 花王株式会社 | 角栓を除去する方法 |
| CN111902720B (zh) | 2018-03-21 | 2025-02-07 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| KR20210110294A (ko) * | 2018-11-07 | 2021-09-07 | 어플라이드 몰레큘라 트랜스포트 인크. | 통과세포외배출용 전달 구조체 및 관련 방법 |
| PL4263568T3 (pl) | 2020-12-18 | 2025-11-24 | Richter Gedeon Nyrt. | Sposoby oczyszczania poddanego ponownemu fałdowaniu białka fuzyjnego peptydu Fc |
| CN115894604B (zh) * | 2022-12-16 | 2024-01-23 | 康日百奥生物科技(苏州)有限公司 | 重组蛋白澄清纯化方法 |
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-
2010
- 2010-06-21 EA EA201270015A patent/EA020621B1/ru not_active IP Right Cessation
- 2010-06-21 JP JP2012517634A patent/JP5808323B2/ja active Active
- 2010-06-21 US US12/820,087 patent/US8952138B2/en active Active
- 2010-06-21 AU AU2010270986A patent/AU2010270986B2/en active Active
- 2010-06-21 SG SG2011095411A patent/SG176963A1/en unknown
- 2010-06-21 MX MX2011013898A patent/MX2011013898A/es active IP Right Grant
- 2010-06-21 KR KR1020127001716A patent/KR101741859B1/ko active Active
- 2010-06-21 CN CN201080028756.7A patent/CN102482321B/zh active Active
- 2010-06-21 WO PCT/US2010/039390 patent/WO2011005488A1/en not_active Ceased
- 2010-06-21 EP EP18167168.6A patent/EP3366692A1/en active Pending
- 2010-06-21 EP EP10747092.4A patent/EP2445923B1/en active Active
- 2010-06-21 CA CA2765881A patent/CA2765881C/en active Active
- 2010-06-21 BR BRPI1011940A patent/BRPI1011940B8/pt active IP Right Grant
-
2011
- 2011-12-13 IL IL216954A patent/IL216954A/en active IP Right Grant
- 2011-12-22 CL CL2011003278A patent/CL2011003278A1/es unknown
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2012
- 2012-01-20 ZA ZA2012/00512A patent/ZA201200512B/en unknown
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2015
- 2015-01-30 US US14/611,037 patent/US20150315232A1/en not_active Abandoned
- 2015-07-07 US US14/793,590 patent/US20150329586A1/en not_active Abandoned
- 2015-09-07 JP JP2015175422A patent/JP6026608B2/ja active Active
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2017
- 2017-02-01 US US15/422,327 patent/US9856287B2/en active Active
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2018
- 2018-02-06 US US15/889,559 patent/US12269843B2/en active Active
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