MX2011013898A - Proteínas replegadas que usan un estado de oxido-reduccion quimicamente controlado. - Google Patents

Proteínas replegadas que usan un estado de oxido-reduccion quimicamente controlado.

Info

Publication number
MX2011013898A
MX2011013898A MX2011013898A MX2011013898A MX2011013898A MX 2011013898 A MX2011013898 A MX 2011013898A MX 2011013898 A MX2011013898 A MX 2011013898A MX 2011013898 A MX2011013898 A MX 2011013898A MX 2011013898 A MX2011013898 A MX 2011013898A
Authority
MX
Mexico
Prior art keywords
refolding proteins
redox state
chemically controlled
concentrations
controlled redox
Prior art date
Application number
MX2011013898A
Other languages
English (en)
Inventor
Joseph Edward Shultz
Roger Hart
Ronald Nixon Keener Iii
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42710673&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2011013898(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MX2011013898A publication Critical patent/MX2011013898A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se describe un método para replegar proteínas expresadas en células no mamíferas presentes en concentraciones de 2.0 g/L o mayores. El método comprende identificar la relación de pares de tioles y la concentración de la solución amortiguadora de óxido-reducción para alcanzar condiciones bajo las cuales se alcanza el plegado eficiente a concentraciones de 2.0 g/L o mayores y se puede emplear sobre un intervalo de volúmenes, incluyendo la escala comercial.
MX2011013898A 2009-06-22 2010-06-21 Proteínas replegadas que usan un estado de oxido-reduccion quimicamente controlado. MX2011013898A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21925709P 2009-06-22 2009-06-22
PCT/US2010/039390 WO2011005488A1 (en) 2009-06-22 2010-06-21 Refolding proteins using a chemically controlled redox state

Publications (1)

Publication Number Publication Date
MX2011013898A true MX2011013898A (es) 2012-05-22

Family

ID=42710673

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011013898A MX2011013898A (es) 2009-06-22 2010-06-21 Proteínas replegadas que usan un estado de oxido-reduccion quimicamente controlado.

Country Status (15)

Country Link
US (5) US8952138B2 (es)
EP (2) EP3366692A1 (es)
JP (2) JP5808323B2 (es)
KR (1) KR101741859B1 (es)
CN (1) CN102482321B (es)
AU (1) AU2010270986B2 (es)
BR (1) BRPI1011940B8 (es)
CA (1) CA2765881C (es)
CL (1) CL2011003278A1 (es)
EA (1) EA020621B1 (es)
IL (1) IL216954A (es)
MX (1) MX2011013898A (es)
SG (1) SG176963A1 (es)
WO (1) WO2011005488A1 (es)
ZA (1) ZA201200512B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG176963A1 (en) * 2009-06-22 2012-02-28 Amgen Inc Refolding proteins using a chemically controlled redox state
MX2011013417A (es) 2009-06-25 2012-03-29 Amgen Inc Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero.
EP2417982A1 (en) * 2010-07-30 2012-02-15 Ferring B.V. Stabilization of gonadotropins
US20150376228A1 (en) * 2013-02-22 2015-12-31 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2017019776A1 (en) * 2015-07-27 2017-02-02 Purdue Research Foundation Tandem folding methods to improve protein folding yield
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
AU2017298984B2 (en) * 2016-07-22 2023-08-31 Amgen Inc. Methods of purifying Fc-containing proteins
CN108276470A (zh) * 2017-01-06 2018-07-13 深圳市新产业生物医学工程股份有限公司 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒
EP3610849A4 (en) 2017-04-10 2021-01-13 Kao Corporation PROCEDURE FOR REMOVING KERATIN PLUGS
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
SG11202104721RA (en) * 2018-11-07 2021-06-29 Applied Molecular Transport Inc Delivery constructs for transcytosis and related methods
CA3200462A1 (en) 2020-12-18 2022-06-23 Sebastian Damerow Methods for the purification of refolded fc-peptide fusion protein
CN115894604B (zh) * 2022-12-16 2024-01-23 康日百奥生物科技(苏州)有限公司 重组蛋白澄清纯化方法

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
JPH06102034B2 (ja) 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
US4468454A (en) 1983-06-10 1984-08-28 E. I. Du Pont De Nemours And Company Antifoggant process
US4572798A (en) * 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
IL86090A (en) 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
GB8807673D0 (en) 1988-03-31 1988-05-05 Lingner & Fischer Gmbh Novel article
JP2796388B2 (ja) 1988-05-13 1998-09-10 アムジエン・インコーポレーテツド Ｇ−ｃｓｆの精製方法
GB8927546D0 (en) 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
ES2119779T3 (es) 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd Solubilizacion de proteinas en formas activas.
WO1993018136A1 (en) 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
US5663304A (en) 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
EP0657466B1 (en) 1993-11-10 1997-01-15 Pfizer Inc. A process for refolding of (pro-)chymosin, comprising recycling of urea
US5466377A (en) 1994-01-19 1995-11-14 Grandics; Peter Chromatography media and their uses
WO1995032216A1 (en) 1994-05-25 1995-11-30 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
WO1996040912A1 (en) 1995-06-07 1996-12-19 Amgen Inc. Ob protein compositions and method
US5935824A (en) 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
ES2255502T3 (es) 1999-07-21 2006-07-01 Amgen Inc. Polipeptidos vgf y metodos para tratar transtornos relacionados con vgf.
US6808902B1 (en) 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
WO2002020762A2 (en) 2000-09-05 2002-03-14 Amgen Inc. Tnf receptor-like molecules and uses thereof
MXPA03007563A (es) 2001-02-23 2003-12-11 Immunex Corp Recuperacion incrementada de proteinas activas.
US6972327B1 (en) 2001-05-08 2005-12-06 Immunex Corporation Regeneration of chromatography material
US7138370B2 (en) 2001-10-11 2006-11-21 Amgen Inc. Specific binding agents of human angiopoietin-2
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
KR101304718B1 (ko) 2002-12-20 2013-09-05 암겐 인코포레이티드 미오스타틴을 저해하는 결합제
EP1449848A1 (en) 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Method for the production of cystine-knot proteins
WO2005092914A1 (en) 2004-03-22 2005-10-06 Blue Mountain Technology Development Lab Process for promoting proper folding of human serum albumin using a human serum albumin ligand
FR2874318B1 (fr) 2004-08-19 2006-11-24 Oreal Utilisation en cosmetique de composes polysaccharidiques amphoteres a chaine(s) polymerique(s) cationique(s)
BRPI0516011A (pt) 2004-09-24 2008-08-19 Amgen Inc moléculas fc modificadas
US7435804B2 (en) 2004-10-19 2008-10-14 Phage Biotechnology, Inc. Method for obtaining single chain antibodies to human interferon α2b
AU2005299716B2 (en) 2004-10-22 2012-09-06 Amgen Inc. Methods for refolding of recombinant antibodies
ES2579758T3 (es) 2005-01-28 2016-08-16 Zymogenetics, Inc. Preparaciones homogéneas de IL-31
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
US20080032343A1 (en) 2005-12-22 2008-02-07 Genentech, Inc. Recombinant Production of Heparin Binding Proteins
US7612273B2 (en) * 2006-03-20 2009-11-03 Roland Corporation Electronic percussion instrument
EP1845103B1 (en) * 2006-04-10 2015-02-25 Boehringer Ingelheim RCV GmbH & Co KG Method for refolding a protein
RU2439076C2 (ru) 2006-07-14 2012-01-10 Дженентек, Инк. Способ выделения повторно свернутого рекомбинантного белка из культуры прокариотических клеток (варианты)
WO2008096370A2 (en) 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
US20080214795A1 (en) 2007-02-14 2008-09-04 Amgen Inc. Method of isolating antibodies by precipitation
JP2010536341A (ja) 2007-08-15 2010-12-02 アムニクス， インコーポレイテッド 生物学的に活性なポリペプチドの特性を改変するための組成物および方法
PL3216803T3 (pl) 2008-06-25 2020-10-19 Novartis Ag Stabilne i rozpuszczalne przeciwciała hamujące vegf
SG176963A1 (en) 2009-06-22 2012-02-28 Amgen Inc Refolding proteins using a chemically controlled redox state
MX2011013417A (es) * 2009-06-25 2012-03-29 Amgen Inc Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero.
MX2015011430A (es) 2013-03-15 2016-04-20 Amgen Inc Antagonismo de la miostatina en seres humanos.

Also Published As

Publication number Publication date
BRPI1011940A2 (pt) 2016-11-22
JP2012530782A (ja) 2012-12-06
US20100324269A1 (en) 2010-12-23
CN102482321A (zh) 2012-05-30
EA201270015A1 (ru) 2012-05-30
EA020621B1 (ru) 2014-12-30
US20190055281A1 (en) 2019-02-21
US20170145049A1 (en) 2017-05-25
CA2765881C (en) 2016-08-02
EP2445923B1 (en) 2018-09-05
BRPI1011940B1 (pt) 2021-07-20
EP2445923A1 (en) 2012-05-02
CA2765881A1 (en) 2011-01-13
US8952138B2 (en) 2015-02-10
IL216954A (en) 2016-09-29
KR101741859B1 (ko) 2017-06-15
JP2016028065A (ja) 2016-02-25
BRPI1011940B8 (pt) 2021-08-03
JP6026608B2 (ja) 2016-11-16
US9856287B2 (en) 2018-01-02
CN102482321B (zh) 2015-06-17
CL2011003278A1 (es) 2012-11-09
AU2010270986B2 (en) 2014-05-22
JP5808323B2 (ja) 2015-11-10
IL216954A0 (en) 2012-02-29
WO2011005488A1 (en) 2011-01-13
EP3366692A1 (en) 2018-08-29
KR20120052939A (ko) 2012-05-24
ZA201200512B (en) 2012-11-28
US20150315232A1 (en) 2015-11-05
AU2010270986A1 (en) 2012-01-19
SG176963A1 (en) 2012-02-28
US20150329586A1 (en) 2015-11-19

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