JP5808323B2 - 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング - Google Patents

化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング Download PDF

Info

Publication number
JP5808323B2
JP5808323B2 JP2012517634A JP2012517634A JP5808323B2 JP 5808323 B2 JP5808323 B2 JP 5808323B2 JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012517634 A JP2012517634 A JP 2012517634A JP 5808323 B2 JP5808323 B2 JP 5808323B2
Authority
JP
Japan
Prior art keywords
protein
thiol
refolding
buffer
contrast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012517634A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012530782A5 (https=
JP2012530782A (ja
Inventor
シユルツ，ジヨゼフ・エドワード
ハート，ロジヤー
キーナー・ザ・サード，ロナルド・ニクソン
Original Assignee
アムジエン・インコーポレーテツド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42710673&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP5808323(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by アムジエン・インコーポレーテツド filed Critical アムジエン・インコーポレーテツド
Publication of JP2012530782A publication Critical patent/JP2012530782A/ja
Publication of JP2012530782A5 publication Critical patent/JP2012530782A5/ja
Application granted granted Critical
Publication of JP5808323B2 publication Critical patent/JP5808323B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2012517634A 2009-06-22 2010-06-21 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング Active JP5808323B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21925709P 2009-06-22 2009-06-22
US61/219,257 2009-06-22
PCT/US2010/039390 WO2011005488A1 (en) 2009-06-22 2010-06-21 Refolding proteins using a chemically controlled redox state

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015175422A Division JP6026608B2 (ja) 2009-06-22 2015-09-07 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング

Publications (3)

Publication Number Publication Date
JP2012530782A JP2012530782A (ja) 2012-12-06
JP2012530782A5 JP2012530782A5 (https=) 2013-07-18
JP5808323B2 true JP5808323B2 (ja) 2015-11-10

Family

ID=42710673

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2012517634A Active JP5808323B2 (ja) 2009-06-22 2010-06-21 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング
JP2015175422A Active JP6026608B2 (ja) 2009-06-22 2015-09-07 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015175422A Active JP6026608B2 (ja) 2009-06-22 2015-09-07 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング

Country Status (15)

Country Link
US (5) US8952138B2 (https=)
EP (2) EP3366692A1 (https=)
JP (2) JP5808323B2 (https=)
KR (1) KR101741859B1 (https=)
CN (1) CN102482321B (https=)
AU (1) AU2010270986B2 (https=)
BR (1) BRPI1011940B8 (https=)
CA (1) CA2765881C (https=)
CL (1) CL2011003278A1 (https=)
EA (1) EA020621B1 (https=)
IL (1) IL216954A (https=)
MX (1) MX2011013898A (https=)
SG (1) SG176963A1 (https=)
WO (1) WO2011005488A1 (https=)
ZA (1) ZA201200512B (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3366692A1 (en) 2009-06-22 2018-08-29 Amgen, Inc Refolding proteins using a chemically controlled redox state
AU2010266093B2 (en) 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
EP2417982A1 (en) * 2010-07-30 2012-02-15 Ferring B.V. Stabilization of gonadotropins
US20150376228A1 (en) * 2013-02-22 2015-12-31 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2017019776A1 (en) * 2015-07-27 2017-02-02 Purdue Research Foundation Tandem folding methods to improve protein folding yield
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
KR102579850B1 (ko) * 2016-07-22 2023-09-18 암젠 인크 Fc-함유 단백질의 정제 방법
CN108276470A (zh) * 2017-01-06 2018-07-13 深圳市新产业生物医学工程股份有限公司 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒
CN110494120B (zh) * 2017-04-10 2023-07-18 花王株式会社 去除角栓的方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP4083058A3 (en) * 2018-11-07 2023-01-11 Applied Molecular Transport Inc. Delivery constructs for transcytosis and related methods
ES3040791T3 (en) 2020-12-18 2025-11-05 Richter Gedeon Nyrt Methods for the purification of refolded fc-peptide fusion protein
CN115894604B (zh) * 2022-12-16 2024-01-23 康日百奥生物科技(苏州)有限公司 重组蛋白澄清纯化方法

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
EP0122080B1 (en) 1983-03-25 1989-09-13 Celltech Limited A process for the production of a protein
US4468454A (en) 1983-06-10 1984-08-28 E. I. Du Pont De Nemours And Company Antifoggant process
US4572798A (en) * 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
GB8508340D0 (en) 1985-03-29 1985-05-09 Creighton T E Production of protein
US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
IL86090A (en) 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
CA1329119C (en) 1988-03-29 1994-05-03 Milton David Goldenberg Cytotoxic therapy
GB8807673D0 (en) 1988-03-31 1988-05-05 Lingner & Fischer Gmbh Novel article
KR0133561B1 (ko) 1988-05-13 1998-04-21 스티븐 엠. 오드레 과립구 군체 자극인자의 정제방법
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
US5986070A (en) 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
WO1993018136A1 (en) 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
US5663304A (en) 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
EP0657466B1 (en) 1993-11-10 1997-01-15 Pfizer Inc. A process for refolding of (pro-)chymosin, comprising recycling of urea
US5466377A (en) 1994-01-19 1995-11-14 Grandics; Peter Chromatography media and their uses
IL113497A (en) 1994-04-28 2001-01-11 Amgen Inc Method for controlling metallophosphate precipitation in high cell density fermentations
AU2603995A (en) * 1994-05-25 1995-12-18 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
AU6028396A (en) 1995-06-07 1996-12-30 Amgen, Inc. Ob protein compositions and method
AU729540B2 (en) 1996-01-23 2001-02-01 Ortho-Mcneil Pharmaceutical, Inc. Methods for purification and use of erythropoietin binding protein
US5935824A (en) 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US20020052026A1 (en) 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6180391B1 (en) 1998-01-28 2001-01-30 Amgen Inc. Highly efficient controlled expression of exogenous genes in e. coli
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
WO1999060120A2 (en) 1998-05-19 1999-11-25 Avidex Limited Soluble t cell receptor
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
ATE313561T1 (de) 1999-07-21 2006-01-15 Amgen Inc Vgf polypeptide und verfahren zur behandlung von vgf-erkrankungen
US6808902B1 (en) 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
CA2421588C (en) 2000-09-05 2010-01-26 Amgen Inc. Tnf receptor-like molecules and uses thereof
IL157446A0 (en) 2001-02-23 2004-03-28 Immunex Corp Immunex Corp Increased recovery of active proteins
US6972327B1 (en) 2001-05-08 2005-12-06 Immunex Corporation Regeneration of chromatography material
US7138370B2 (en) 2001-10-11 2006-11-21 Amgen Inc. Specific binding agents of human angiopoietin-2
ITMI20012345A1 (it) 2001-11-08 2003-05-08 Internat Ct For Genetic En Gin Procedimento per la produzione di interferone alfa di grado terapeutico
SI21102A (sl) 2001-12-19 2003-06-30 LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
US7067279B1 (en) 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
BR0317538A (pt) 2002-12-20 2005-11-29 Amgen Inc Agente ligante, sequência de polinucleotìdeo, vetor de expressão, célula hospedeira, composição farmacêutica, e, métodos de inibir a atividade de miostatina, de aumentar a massa muscular magra e a razão de massa muscular magra para gordura, de tratar uma doença de emaciação muscular e um distúrbio metabólico relacionado com miostatina em um indivìduo, de detectar e medir miostatina em uma amostra, e, de diagnosticar um distúrbio relacionado com miostatina em um indivìduo
US7083948B1 (en) 2002-12-24 2006-08-01 Immunex Corporation Polypeptide purification reagents and methods for their use
EP1449848A1 (en) 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Method for the production of cystine-knot proteins
GB0304068D0 (en) 2003-02-22 2003-03-26 Avidex Ltd Substances
US7427659B2 (en) 2003-10-24 2008-09-23 Amgen Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
EP1711512B1 (en) 2004-01-30 2012-01-11 Amgen Inc. Process for purifying proteins
AU2005222782B2 (en) 2004-02-04 2010-06-17 Ratiopharm Gmbh Methods of refolding mammalian glycosyltransferases
US20050209441A1 (en) 2004-03-22 2005-09-22 Lile Jackson D Process for promoting proper folding of human serum albumin using a human serum albumin ligand
CA2577690C (en) 2004-08-19 2013-08-06 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
FR2874318B1 (fr) 2004-08-19 2006-11-24 Oreal Utilisation en cosmetique de composes polysaccharidiques amphoteres a chaine(s) polymerique(s) cationique(s)
DE102004041639A1 (de) 2004-08-27 2006-03-02 Bioceuticals Arzneimittel Ag Verfahren zur Gewinnung von biologisch aktivem humanen G-CSF aus Inclusion Bodies
WO2006036834A2 (en) 2004-09-24 2006-04-06 Amgen Inc. MODIFIED Fc MOLECULES
US7435804B2 (en) 2004-10-19 2008-10-14 Phage Biotechnology, Inc. Method for obtaining single chain antibodies to human interferon α2b
AU2005299716B2 (en) 2004-10-22 2012-09-06 Amgen Inc. Methods for refolding of recombinant antibodies
AU2005314438B2 (en) 2004-12-10 2012-03-29 Zymogenetics, Inc. FGF18 production in prokaryotic hosts
JP2008528039A (ja) 2005-01-28 2008-07-31 ザイモジェネティクス，インコーポレイティド Ｉｌ−３１の均質調製物
WO2006097944A2 (en) 2005-03-17 2006-09-21 Zenotech Laboratories Limited Process for the purification of recombinant granulocyte-colony stimulating factor
US7344962B2 (en) 2005-06-21 2008-03-18 International Business Machines Corporation Method of manufacturing dual orientation wafers
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
US8008453B2 (en) 2005-08-12 2011-08-30 Amgen Inc. Modified Fc molecules
US7735525B2 (en) 2005-11-14 2010-06-15 Amgen Inc. Thermally insulated apparatus for liquid chromatographic analysis
WO2007075283A2 (en) 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
BRPI0621124A2 (pt) 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
US7790862B2 (en) 2006-06-13 2010-09-07 Zymogenetics, Inc. IL-17 and IL-23 antagonists and methods of using the same
US7612273B2 (en) * 2006-03-20 2009-11-03 Roland Corporation Electronic percussion instrument
EP1845103B1 (en) * 2006-04-10 2015-02-25 Boehringer Ingelheim RCV GmbH & Co KG Method for refolding a protein
AR062069A1 (es) 2006-07-14 2008-10-15 Genentech Inc Replegado de proteinas recombinantes
EP2049821A4 (en) 2006-07-21 2013-09-04 Amgen Inc Rupture valve
TW201307390A (zh) 2007-02-02 2013-02-16 Amgen Inc 海帕西啶（ｈｅｐｃｉｄｉｎ）、海帕西啶拮抗劑及使用方法
WO2008097829A2 (en) 2007-02-02 2008-08-14 Neose Technologies, Inc. Large scale production of eukaryotic n-acetylglucosaminyltransferase i in bacteria
WO2008096370A2 (en) 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
WO2008100578A2 (en) 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation
JP2010536341A (ja) 2007-08-15 2010-12-02 アムニクス， インコーポレイテッド 生物学的に活性なポリペプチドの特性を改変するための組成物および方法
CN102015770A (zh) 2008-02-29 2011-04-13 特拉维夫大学拉莫特有限公司 免疫球蛋白组合物和生产其的方法
BRPI0914251B1 (pt) 2008-06-25 2022-07-19 Novartis Ag Imunoligante recombinante, seu fragmento de ligação ao antígeno, uso dos mesmos, e composição
EP3366692A1 (en) 2009-06-22 2018-08-29 Amgen, Inc Refolding proteins using a chemically controlled redox state
AU2010266093B2 (en) * 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
TWI582408B (zh) 2011-08-29 2017-05-11 安美基公司 用於非破壞性檢測－流體中未溶解粒子之方法及裝置
US8945872B2 (en) 2013-01-25 2015-02-03 Warsaw Orthopedic, Inc. Methods of purifying human recombinant growth and differentiation factor-5 (rhGDF-5) protein
US20160038588A1 (en) 2013-03-15 2016-02-11 Amgen Inc. Myostatin Antagonism in Human Subjects
US9632095B2 (en) 2014-12-01 2017-04-25 University Of Delaware Device and method for determining reaction kinetics
US9704239B1 (en) 2016-09-02 2017-07-11 Amgen Inc. Video trigger synchronization for improved particle detection in a vessel

Also Published As

Publication number Publication date
US20100324269A1 (en) 2010-12-23
CA2765881C (en) 2016-08-02
KR20120052939A (ko) 2012-05-24
US20150329586A1 (en) 2015-11-19
EA201270015A1 (ru) 2012-05-30
BRPI1011940B8 (pt) 2021-08-03
ZA201200512B (en) 2012-11-28
IL216954A (en) 2016-09-29
WO2011005488A1 (en) 2011-01-13
AU2010270986A1 (en) 2012-01-19
US20170145049A1 (en) 2017-05-25
IL216954A0 (en) 2012-02-29
CA2765881A1 (en) 2011-01-13
JP6026608B2 (ja) 2016-11-16
CL2011003278A1 (es) 2012-11-09
US20190055281A1 (en) 2019-02-21
US12269843B2 (en) 2025-04-08
EP2445923B1 (en) 2018-09-05
BRPI1011940B1 (pt) 2021-07-20
US8952138B2 (en) 2015-02-10
SG176963A1 (en) 2012-02-28
KR101741859B1 (ko) 2017-06-15
JP2016028065A (ja) 2016-02-25
US9856287B2 (en) 2018-01-02
EP3366692A1 (en) 2018-08-29
CN102482321A (zh) 2012-05-30
EA020621B1 (ru) 2014-12-30
AU2010270986B2 (en) 2014-05-22
EP2445923A1 (en) 2012-05-02
BRPI1011940A2 (pt) 2016-11-22
JP2012530782A (ja) 2012-12-06
MX2011013898A (es) 2012-05-22
CN102482321B (zh) 2015-06-17
US20150315232A1 (en) 2015-11-05

Similar Documents

Publication Publication Date Title
JP6026608B2 (ja) 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング
Qoronfleh et al. Confronting high-throughput protein refolding using high pressure and solution screens
US11345722B2 (en) High pH protein refolding methods
US11053278B2 (en) Tangential flow filtration based protein refolding methods
EP3337820B1 (en) An improved refolding process for antibody's fragments
HK1259915A1 (en) Refolding proteins using a chemically controlled redox state
HK1170245B (en) Refolding proteins using a chemically controlled redox state
HK1170245A (en) Refolding proteins using a chemically controlled redox state

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130603

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130603

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20141104

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150129

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150501

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150818

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150908

R150 Certificate of patent or registration of utility model

Ref document number: 5808323

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250