JP2012531428A5 - - Google Patents
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- JP2012531428A5 JP2012531428A5 JP2012517737A JP2012517737A JP2012531428A5 JP 2012531428 A5 JP2012531428 A5 JP 2012531428A5 JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012531428 A5 JP2012531428 A5 JP 2012531428A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- separation matrix
- solution
- refolding
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 27
- 108090000623 proteins and genes Proteins 0.000 claims 19
- 102000004169 proteins and genes Human genes 0.000 claims 19
- 235000018102 proteins Nutrition 0.000 claims 18
- 239000011159 matrix material Substances 0.000 claims 14
- 238000000926 separation method Methods 0.000 claims 13
- 239000000243 solution Substances 0.000 claims 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 6
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 6
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 5
- 239000011347 resin Substances 0.000 claims 5
- 229920005989 resin Polymers 0.000 claims 5
- 238000005406 washing Methods 0.000 claims 5
- 239000003638 chemical reducing agent Substances 0.000 claims 4
- 230000008929 regeneration Effects 0.000 claims 4
- 238000011069 regeneration method Methods 0.000 claims 4
- 108010024636 Glutathione Proteins 0.000 claims 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims 3
- 239000007983 Tris buffer Substances 0.000 claims 3
- 239000004202 carbamide Substances 0.000 claims 3
- 239000013592 cell lysate Substances 0.000 claims 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 3
- 235000018417 cysteine Nutrition 0.000 claims 3
- 239000003398 denaturant Substances 0.000 claims 3
- 239000004475 Arginine Substances 0.000 claims 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 2
- RYECOJGRJDOGPP-UHFFFAOYSA-N Ethylurea Chemical compound CCNC(N)=O RYECOJGRJDOGPP-UHFFFAOYSA-N 0.000 claims 2
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 2
- MGJKQDOBUOMPEZ-UHFFFAOYSA-N N,N'-dimethylurea Chemical compound CNC(=O)NC MGJKQDOBUOMPEZ-UHFFFAOYSA-N 0.000 claims 2
- XGEGHDBEHXKFPX-UHFFFAOYSA-N N-methylthiourea Natural products CNC(N)=O XGEGHDBEHXKFPX-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 230000002776 aggregation Effects 0.000 claims 2
- 238000004220 aggregation Methods 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 229960003180 glutathione Drugs 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 239000002563 ionic surfactant Substances 0.000 claims 2
- 230000002934 lysing effect Effects 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- XGEGHDBEHXKFPX-NJFSPNSNSA-N methylurea Chemical compound [14CH3]NC(N)=O XGEGHDBEHXKFPX-NJFSPNSNSA-N 0.000 claims 2
- 239000002736 nonionic surfactant Substances 0.000 claims 2
- 230000003204 osmotic effect Effects 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 2
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 2
- 235000011151 potassium sulphates Nutrition 0.000 claims 2
- 229940124272 protein stabilizer Drugs 0.000 claims 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 2
- 235000011152 sodium sulphate Nutrition 0.000 claims 2
- 238000005063 solubilization Methods 0.000 claims 2
- 230000007928 solubilization Effects 0.000 claims 2
- 230000003381 solubilizing effect Effects 0.000 claims 2
- 239000000600 sorbitol Substances 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 150000005846 sugar alcohols Polymers 0.000 claims 2
- 239000004094 surface-active agent Substances 0.000 claims 2
- 210000005253 yeast cell Anatomy 0.000 claims 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 1
- 108010053070 Glutathione Disulfide Proteins 0.000 claims 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- 101710120037 Toxin CcdB Proteins 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- -1 antibodies Proteins 0.000 claims 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 claims 1
- 229940099500 cystamine Drugs 0.000 claims 1
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 claims 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 claims 1
- 230000002209 hydrophobic effect Effects 0.000 claims 1
- 210000003000 inclusion body Anatomy 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 239000003456 ion exchange resin Substances 0.000 claims 1
- 229920003303 ion-exchange polymer Polymers 0.000 claims 1
- 229960003151 mercaptamine Drugs 0.000 claims 1
- YPZRWBKMTBYPTK-UHFFFAOYSA-N oxidized gamma-L-glutamyl-L-cysteinylglycine Natural products OC(=O)C(N)CCC(=O)NC(C(=O)NCC(O)=O)CSSCC(C(=O)NCC(O)=O)NC(=O)CCC(N)C(O)=O YPZRWBKMTBYPTK-UHFFFAOYSA-N 0.000 claims 1
- 229940016590 sarkosyl Drugs 0.000 claims 1
- 108700004121 sarkosyl Proteins 0.000 claims 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22047709P | 2009-06-25 | 2009-06-25 | |
| US61/220,477 | 2009-06-25 | ||
| PCT/US2010/039854 WO2010151688A2 (en) | 2009-06-25 | 2010-06-24 | Capture purification processes for proteins expressed in a non-mammalian system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012531428A JP2012531428A (ja) | 2012-12-10 |
| JP2012531428A5 true JP2012531428A5 (https=) | 2013-08-08 |
Family
ID=43242573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012517737A Pending JP2012531428A (ja) | 2009-06-25 | 2010-06-24 | 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US8940878B2 (https=) |
| EP (2) | EP2445924B2 (https=) |
| JP (1) | JP2012531428A (https=) |
| AU (1) | AU2010266093B2 (https=) |
| CA (1) | CA2765154A1 (https=) |
| DK (1) | DK3660032T3 (https=) |
| ES (2) | ES2862580T5 (https=) |
| FI (1) | FI3660032T3 (https=) |
| LT (1) | LT3660032T (https=) |
| MX (1) | MX2011013417A (https=) |
| SI (1) | SI3660032T1 (https=) |
| WO (1) | WO2010151688A2 (https=) |
Families Citing this family (11)
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| EP3366692A1 (en) | 2009-06-22 | 2018-08-29 | Amgen, Inc | Refolding proteins using a chemically controlled redox state |
| AU2010266093B2 (en) | 2009-06-25 | 2013-06-27 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| EP2542574B1 (en) | 2010-03-04 | 2017-08-09 | Pfenex Inc. | Method for producing soluble recombinant interferon protein without denaturing |
| GB201506870D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
| US20170101435A1 (en) * | 2015-10-13 | 2017-04-13 | Therapeutic Proteins International, LLC | Harvesting and perfusion apparatus |
| KR102579850B1 (ko) * | 2016-07-22 | 2023-09-18 | 암젠 인크 | Fc-함유 단백질의 정제 방법 |
| MA49962A (fr) | 2017-08-22 | 2020-07-01 | Biogen Ma Inc | Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé |
| US11051532B2 (en) * | 2017-09-22 | 2021-07-06 | Impossible Foods Inc. | Methods for purifying protein |
| SG11202102713TA (en) * | 2018-09-21 | 2021-04-29 | Teneobio Inc | Methods for purifying heterodimeric, multispecific antibodies |
| US12011016B2 (en) | 2020-09-14 | 2024-06-18 | Impossible Foods Inc. | Protein methods and compositions |
| ES3040791T3 (en) | 2020-12-18 | 2025-11-05 | Richter Gedeon Nyrt | Methods for the purification of refolded fc-peptide fusion protein |
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-
2010
- 2010-06-24 AU AU2010266093A patent/AU2010266093B2/en not_active Ceased
- 2010-06-24 WO PCT/US2010/039854 patent/WO2010151688A2/en not_active Ceased
- 2010-06-24 LT LTEP19209054.6T patent/LT3660032T/lt unknown
- 2010-06-24 US US12/822,990 patent/US8940878B2/en active Active
- 2010-06-24 SI SI201032164T patent/SI3660032T1/sl unknown
- 2010-06-24 MX MX2011013417A patent/MX2011013417A/es active IP Right Grant
- 2010-06-24 EP EP10729997.6A patent/EP2445924B2/en active Active
- 2010-06-24 CA CA2765154A patent/CA2765154A1/en not_active Abandoned
- 2010-06-24 FI FIEP19209054.6T patent/FI3660032T3/fi active
- 2010-06-24 EP EP19209054.6A patent/EP3660032B1/en active Active
- 2010-06-24 ES ES10729997T patent/ES2862580T5/es active Active
- 2010-06-24 JP JP2012517737A patent/JP2012531428A/ja active Pending
- 2010-06-24 DK DK19209054.6T patent/DK3660032T3/da active
- 2010-06-24 ES ES19209054T patent/ES3056593T3/es active Active
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2015
- 2015-01-16 US US14/599,336 patent/US9643997B2/en active Active
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2019
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2022
- 2022-07-27 US US17/874,846 patent/US12312381B2/en active Active
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