WO2006041849A3 - Methods and compositions for concentrating secreted recombinant protein - Google Patents

Methods and compositions for concentrating secreted recombinant protein Download PDF

Info

Publication number
WO2006041849A3
WO2006041849A3 PCT/US2005/035697 US2005035697W WO2006041849A3 WO 2006041849 A3 WO2006041849 A3 WO 2006041849A3 US 2005035697 W US2005035697 W US 2005035697W WO 2006041849 A3 WO2006041849 A3 WO 2006041849A3
Authority
WO
WIPO (PCT)
Prior art keywords
chitin
compositions
methods
recombinant protein
proteins
Prior art date
Application number
PCT/US2005/035697
Other languages
French (fr)
Other versions
WO2006041849A2 (en
Inventor
Christopher H Taron
Paul A Colussi
Original Assignee
New England Biolabs Inc
Christopher H Taron
Paul A Colussi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc, Christopher H Taron, Paul A Colussi filed Critical New England Biolabs Inc
Priority to CN2005800392100A priority Critical patent/CN101061225B/en
Priority to JP2007535756A priority patent/JP2008515427A/en
Priority to EP05810030A priority patent/EP1797187A2/en
Priority to AU2005294467A priority patent/AU2005294467B2/en
Priority to CA002582808A priority patent/CA2582808A1/en
Publication of WO2006041849A2 publication Critical patent/WO2006041849A2/en
Publication of WO2006041849A3 publication Critical patent/WO2006041849A3/en
Priority to IL182442A priority patent/IL182442A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand

Abstract

Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
PCT/US2005/035697 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant protein WO2006041849A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN2005800392100A CN101061225B (en) 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant protein
JP2007535756A JP2008515427A (en) 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant proteins
EP05810030A EP1797187A2 (en) 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant protein
AU2005294467A AU2005294467B2 (en) 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant protein
CA002582808A CA2582808A1 (en) 2004-10-06 2005-10-03 Methods and composition for concentrating secreted recombinant proteins
IL182442A IL182442A0 (en) 2004-10-06 2007-04-10 Methods and compositions for concentrating secreted recombinant protein

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61642004P 2004-10-06 2004-10-06
US60/616,420 2004-10-06
US69047005P 2005-06-14 2005-06-14
US60/690,470 2005-06-14

Publications (2)

Publication Number Publication Date
WO2006041849A2 WO2006041849A2 (en) 2006-04-20
WO2006041849A3 true WO2006041849A3 (en) 2006-12-07

Family

ID=35636770

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/035697 WO2006041849A2 (en) 2004-10-06 2005-10-03 Methods and compositions for concentrating secreted recombinant protein

Country Status (8)

Country Link
EP (1) EP1797187A2 (en)
JP (1) JP2008515427A (en)
KR (1) KR20070085295A (en)
CN (1) CN101061225B (en)
AU (1) AU2005294467B2 (en)
CA (1) CA2582808A1 (en)
IL (1) IL182442A0 (en)
WO (1) WO2006041849A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7732565B2 (en) 2005-09-20 2010-06-08 New England Biolabs, Inc. Compositions and methods relating to elutable carbohydrate-binding proteins
US8729233B2 (en) 2010-08-30 2014-05-20 Board Of Trustees Of Michigan State University Microbial nanowires and products related thereto
WO2013033456A2 (en) 2011-09-02 2013-03-07 Board Of Trustees Of Michigan State University Microbial nanowires and methods of making and using
KR101710885B1 (en) * 2015-01-22 2017-02-28 광운대학교 산학협력단 Protein preconcentration device using capillary and fabrication method thereof
CN107236681A (en) * 2017-06-20 2017-10-10 江西嘉博生物工程有限公司 A kind of Kluyveromyces lactis and expression for expressing pig lysozyme gene

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003074660A2 (en) * 2002-02-28 2003-09-12 New England Biolabs, Inc. Modified chitin binding domain and uses thereof
WO2005100586A2 (en) * 2004-04-08 2005-10-27 New England Biolabs, Inc. Method for construction and use of kluyveromyces lactis promoter variants in k.lactis that substantially lack e. coli transcriptional capability

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003074660A2 (en) * 2002-02-28 2003-09-12 New England Biolabs, Inc. Modified chitin binding domain and uses thereof
WO2005100586A2 (en) * 2004-04-08 2005-10-27 New England Biolabs, Inc. Method for construction and use of kluyveromyces lactis promoter variants in k.lactis that substantially lack e. coli transcriptional capability

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DICKSON R C ET AL: "THE LACTOSE GALACTOSE REGULON KLUYVEROMYCES-LACTIS", BARR, P. J., A. J. BRAKE AND P. VALENZUELA (ED.). BIOTECHNOLOGY SERIES, 13. YEAST GENETIC ENGINEERING. XVIII+354P. BUTTERWORTHS: STONEHAM, MASSACHUSETTS, USA; LONDON, ENGLAND, UK. ILLUS SERIES : BIOTECHNOLOGY SERIES (ISSN 0740-7378), 1989, pages 19 - 40, XP008062660, ISSN: 0-409-90117-2 *
GIBBS M D ET AL: "A yeast intron as a translational terminator in a plasmid shuttle vector", FEMS YEAST RESEARCH, ELSEVIER SCIENCE, TOKYO, NL, vol. 4, no. 6, March 2004 (2004-03-01), pages 573 - 577, XP002992262, ISSN: 1567-1356 *
ROSE M ET AL: "STRUCTURE AND FUNCTION OF THE YEAST URA-3 GENE EXPRESSION IN ESCHERICHIA-COLI", GENE (AMSTERDAM), vol. 29, no. 1-2, 1984, pages 113 - 124, XP002376192, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
AU2005294467B2 (en) 2011-03-31
CN101061225B (en) 2013-03-27
IL182442A0 (en) 2007-07-24
JP2008515427A (en) 2008-05-15
CA2582808A1 (en) 2006-04-20
WO2006041849A2 (en) 2006-04-20
EP1797187A2 (en) 2007-06-20
KR20070085295A (en) 2007-08-27
CN101061225A (en) 2007-10-24
AU2005294467A1 (en) 2006-04-20

Similar Documents

Publication Publication Date Title
Ruiz-Ruiz et al. Aqueous two-phase affinity partitioning systems: current applications and trends
WO2006041849A3 (en) Methods and compositions for concentrating secreted recombinant protein
WO2008008975A3 (en) Refolding of recombinant proteins
WO2007112005A3 (en) Pc5 as a factor ix propeptide processing enzyme
MX2009010492A (en) Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells.
WO2007096192A3 (en) Production of biologically active proteins
WO2007086879A3 (en) Method of expressing proteins with disulfide bridges
WO2006136084A8 (en) Fusion proteins of recombinant sars coronavirus structural proteins, their production and uses
WO2007075873A3 (en) Protein engineering strategies to optimize activity of surface attached proteins
EP2172484A3 (en) Serum albumin binding proteins
WO2006069403A3 (en) Methods for producing soluble multi-membrane-spanning proteins
WO2012046061A3 (en) Clostridium difficile antigens
MX2019015304A (en) Cation exchange chromatography wash buffer.
WO2008104890A3 (en) Compositions and methods for producing apolipoprotein
MX342235B (en) Optimized method for antibody capturing by mixed mode chromatography.
WO2006060728A3 (en) Methods and compositions involving lcrv proteins
AU2010291066A8 (en) Protein glycosylation
Ribeiro et al. Enzyme-enhanced extraction of phenolic compounds and proteins from flaxseed meal
JP2012531428A5 (en)
WO2007130154A8 (en) Recombinant production of heparin binding proteins
WO2009015367A3 (en) Stabilizing proteins using ionic liquids
Margaritis et al. Xylanase, CM-cellulase and avicelase production by the thermophilic fungus Sporotrichum thermophile
Wang et al. A novel strategy for the preparation of codon-optimized truncated Ulp1 and its simplified application to cleavage the SUMO fusion protein
WO2011142529A3 (en) Soluble expression of bulky folded active proteins
WO2007096899A3 (en) Affinity polypeptide for purification of recombinant proteins

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV LY MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2005294467

Country of ref document: AU

Ref document number: 2005810030

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2582808

Country of ref document: CA

Ref document number: 488/MUMNP/2007

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 2007535756

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 182442

Country of ref document: IL

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2005294467

Country of ref document: AU

Date of ref document: 20051003

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2005294467

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1020077010251

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 200580039210.0

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 2005810030

Country of ref document: EP