WO2009015367A3 - Stabilizing proteins using ionic liquids - Google Patents

Stabilizing proteins using ionic liquids Download PDF

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Publication number
WO2009015367A3
WO2009015367A3 PCT/US2008/071254 US2008071254W WO2009015367A3 WO 2009015367 A3 WO2009015367 A3 WO 2009015367A3 US 2008071254 W US2008071254 W US 2008071254W WO 2009015367 A3 WO2009015367 A3 WO 2009015367A3
Authority
WO
WIPO (PCT)
Prior art keywords
ionic liquids
stabilizing proteins
stabilizing
compositions
protein
Prior art date
Application number
PCT/US2008/071254
Other languages
French (fr)
Other versions
WO2009015367A2 (en
Inventor
C Austen Angell
Nolene Byrne
Jean-Philippe Belieres
Limin Wang
Original Assignee
Univ Arizona State
C Austen Angell
Nolene Byrne
Jean-Philippe Belieres
Limin Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arizona State, C Austen Angell, Nolene Byrne, Jean-Philippe Belieres, Limin Wang filed Critical Univ Arizona State
Publication of WO2009015367A2 publication Critical patent/WO2009015367A2/en
Publication of WO2009015367A3 publication Critical patent/WO2009015367A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Among other things, techniques and compositions for stabilizing proteins are disclosed. To stabilizing a protein, lyophilized protein is dissolved in a low-viscous solvent that includes water and ionic liquid. Compositions and techniques relate to reversible folding-unfolding, aggregation protection and multi-year stabilization in high protein concentration solutions of protic and/or aprotic ionic liquids.
PCT/US2008/071254 2007-07-25 2008-07-25 Stabilizing proteins using ionic liquids WO2009015367A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95188607P 2007-07-25 2007-07-25
US60/951,886 2007-07-25

Publications (2)

Publication Number Publication Date
WO2009015367A2 WO2009015367A2 (en) 2009-01-29
WO2009015367A3 true WO2009015367A3 (en) 2009-06-25

Family

ID=40282177

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/071254 WO2009015367A2 (en) 2007-07-25 2008-07-25 Stabilizing proteins using ionic liquids

Country Status (1)

Country Link
WO (1) WO2009015367A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US9289381B2 (en) 2009-12-03 2016-03-22 The University Of North Carolina At Charlotte Stabilization and storage of biological pharmaceutical compositions
US10497970B2 (en) 2013-03-14 2019-12-03 Arizona Board Of Regents On Behalf Of Arizona State University Alkali ion conducting plastic crystals
RU2675824C2 (en) 2013-09-11 2018-12-25 Игл Байолоджикс, Инк. Liquid protein formulations containing ionic liquids
KR102497368B1 (en) 2014-10-01 2023-02-10 이글 바이올로직스 인코포레이티드 Polysaccharide and nucleic acid formulations containing viscosity-lowering agents
CN116286771B (en) * 2023-02-03 2024-02-23 上海碧云天生物技术股份有限公司 Broad-spectrum protein stable preservation solution, preparation method and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGELL C. ET AL.: "Parallel Developments in Aprotic and Protic Ionic Liquids: Physical Chemistry and Applications", ACC. CHEM. RES., vol. 40, no. 11, 2007, pages 1228 - 1236 *
BYRNE N. ET AL.: "Protein Unfolding, and the ''Tuning In'' of Reversible Intermediate States, in Protic Ionic Liquid Media", JOURNAL OF MOLECULAR BIOLOGY., vol. 378, no. 3, May 2008 (2008-05-01), pages 707 - 714, XP022762546, DOI: doi:10.1016/j.jmb.2008.02.050 *
BYRNE N. ET AL.: "Reversible Folding-unfolding, Aggregation Protection, and Multi-year Stabilization, in High Concentration Protein Solutions, Using Ionic Liquids", CHEMICAL COMMUNICATIONS, vol. 26, 19 April 2007 (2007-04-19), pages 2714 - 2716 *
TAMAR L. ET AL.: "Many Protic Ionic Liquids Mediate Hydrocarbon-Solvent Interactions and Promote Amphiphile Self-Assembly", LANGMUIR., vol. 23, no. 2, 2007, pages 402 - 404 *

Also Published As

Publication number Publication date
WO2009015367A2 (en) 2009-01-29

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