WO2007075873A3 - Protein engineering strategies to optimize activity of surface attached proteins - Google Patents

Protein engineering strategies to optimize activity of surface attached proteins Download PDF

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Publication number
WO2007075873A3
WO2007075873A3 PCT/US2006/048764 US2006048764W WO2007075873A3 WO 2007075873 A3 WO2007075873 A3 WO 2007075873A3 US 2006048764 W US2006048764 W US 2006048764W WO 2007075873 A3 WO2007075873 A3 WO 2007075873A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein engineering
surface attached
engineering strategies
optimize activity
attached proteins
Prior art date
Application number
PCT/US2006/048764
Other languages
French (fr)
Other versions
WO2007075873A2 (en
Inventor
David Hanzel
Jonas Korlach
Paul Peluso
Geoff Otto
Thang Pham
David Rank
Stephen Turner
Original Assignee
Pacific Biosciences California
David Hanzel
Jonas Korlach
Paul Peluso
Geoff Otto
Thang Pham
David Rank
Stephen Turner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences California, David Hanzel, Jonas Korlach, Paul Peluso, Geoff Otto, Thang Pham, David Rank, Stephen Turner filed Critical Pacific Biosciences California
Priority to EP06848977A priority Critical patent/EP1963501A4/en
Priority to CA 2633520 priority patent/CA2633520A1/en
Priority to AU2006331677A priority patent/AU2006331677A1/en
Publication of WO2007075873A2 publication Critical patent/WO2007075873A2/en
Publication of WO2007075873A3 publication Critical patent/WO2007075873A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2993—Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2993—Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
    • Y10T428/2996—Glass particles or spheres
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31652—Of asbestos
    • Y10T428/31663—As siloxane, silicone or silane
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31725—Of polyamide
    • Y10T428/31768—Natural source-type polyamide [e.g., casein, gelatin, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.
PCT/US2006/048764 2005-12-22 2006-12-21 Protein engineering strategies to optimize activity of surface attached proteins WO2007075873A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP06848977A EP1963501A4 (en) 2005-12-22 2006-12-21 Protein engineering strategies to optimize activity of surface attached proteins
CA 2633520 CA2633520A1 (en) 2005-12-22 2006-12-21 Protein engineering strategies to optimize activity of surface attached proteins
AU2006331677A AU2006331677A1 (en) 2005-12-22 2006-12-21 Protein engineering strategies to optimize activity of surface attached proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75344605P 2005-12-22 2005-12-22
US60/753,446 2005-12-22

Publications (2)

Publication Number Publication Date
WO2007075873A2 WO2007075873A2 (en) 2007-07-05
WO2007075873A3 true WO2007075873A3 (en) 2007-11-29

Family

ID=38218602

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/048764 WO2007075873A2 (en) 2005-12-22 2006-12-21 Protein engineering strategies to optimize activity of surface attached proteins

Country Status (5)

Country Link
US (1) US20100260465A1 (en)
EP (1) EP1963501A4 (en)
AU (1) AU2006331677A1 (en)
CA (1) CA2633520A1 (en)
WO (1) WO2007075873A2 (en)

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US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
EP1963536B1 (en) 2005-12-22 2016-05-04 Pacific Biosciences of California, Inc. Polymerases for nucleotide analogue incorporation
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
EP2203566A4 (en) 2007-09-28 2011-02-09 Pacific Biosciences California Error-free amplification of dna for clonal sequencing
EP2217928B1 (en) * 2007-12-04 2017-08-09 Pacific Biosciences of California, Inc. Alternate labeling strategies for single molecule sequencing
CA2718404C (en) 2008-03-13 2018-04-10 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
EP2274449B1 (en) 2008-03-28 2015-12-23 Pacific Biosciences of California, Inc. Methods for nucleic acid sequencing
CA2720247C (en) 2008-03-31 2020-07-14 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
US9637380B2 (en) 2008-03-31 2017-05-02 Pacific Biosciences Of California, Inc. Nanoscale apertures having islands of functionality
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US8367159B2 (en) 2009-09-11 2013-02-05 Pacific Biosciences Of California, Inc. Methods for producing ZMWs with islands of functionality
US8906670B2 (en) 2009-09-11 2014-12-09 Pacific Bioscience Of California, Inc. Zero-mode waveguides with non-reflecting walls
WO2012027618A2 (en) 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Functionalized cyanine dyes
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
FR2971080B1 (en) 2011-02-02 2013-03-01 Alstom Grid Sas VACUUM BULB DEVICE COMPRISING A LOCKING MEANS
US9868945B2 (en) 2011-02-08 2018-01-16 Life Technologies Corporation Linking methods, compositions, systems, kits and apparatuses
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US8906660B2 (en) 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
WO2013131888A1 (en) 2012-03-06 2013-09-12 Rudolf Rigler Cyclic single molecule sequencing process
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
WO2013173844A1 (en) 2012-05-18 2013-11-21 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
WO2016028887A1 (en) 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
WO2017024049A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Single-molecule nanofet sequencing systems and methods
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
EP3376997A4 (en) 2015-11-20 2019-04-24 Pacific Biosciences Of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
CN117024498A (en) 2015-11-20 2023-11-10 加利福尼亚太平洋生物科学股份有限公司 Protected dye-labeled reagent
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors

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Also Published As

Publication number Publication date
US20100260465A1 (en) 2010-10-14
WO2007075873A2 (en) 2007-07-05
EP1963501A4 (en) 2009-04-22
AU2006331677A1 (en) 2007-07-05
EP1963501A2 (en) 2008-09-03
CA2633520A1 (en) 2007-07-05

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