JP2012531428A - 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス - Google Patents

哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス Download PDF

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JP2012531428A
JP2012531428A JP2012517737A JP2012517737A JP2012531428A JP 2012531428 A JP2012531428 A JP 2012531428A JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012517737 A JP2012517737 A JP 2012517737A JP 2012531428 A JP2012531428 A JP 2012531428A
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protein
separation matrix
proteins
refolding
solution
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JP2012531428A5 (https=
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シユルツ，ジヨゼフ・エドワード
ハート，ロジヤー
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アムジエン・インコーポレーテツド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2012517737A 2009-06-25 2010-06-24 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス Pending JP2012531428A (ja)

Applications Claiming Priority (3)

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US22047709P 2009-06-25 2009-06-25
US61/220,477 2009-06-25
PCT/US2010/039854 WO2010151688A2 (en) 2009-06-25 2010-06-24 Capture purification processes for proteins expressed in a non-mammalian system

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JP2012531428A true JP2012531428A (ja) 2012-12-10
JP2012531428A5 JP2012531428A5 (https=) 2013-08-08

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US (5) US8940878B2 (https=)
EP (2) EP2445924B2 (https=)
JP (1) JP2012531428A (https=)
AU (1) AU2010266093B2 (https=)
CA (1) CA2765154A1 (https=)
DK (1) DK3660032T3 (https=)
ES (2) ES2862580T5 (https=)
FI (1) FI3660032T3 (https=)
LT (1) LT3660032T (https=)
MX (1) MX2011013417A (https=)
SI (1) SI3660032T1 (https=)
WO (1) WO2010151688A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190037247A (ko) * 2016-07-22 2019-04-05 암젠 인크 Fc-함유 단백질의 정제 방법
JP2022501357A (ja) * 2018-09-21 2022-01-06 テネオバイオ， インコーポレイテッド ヘテロ二量体多重特異性抗体を精製するための方法

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EP3366692A1 (en) 2009-06-22 2018-08-29 Amgen, Inc Refolding proteins using a chemically controlled redox state
AU2010266093B2 (en) 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
EP2542574B1 (en) 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
GB201506870D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
MA49962A (fr) 2017-08-22 2020-07-01 Biogen Ma Inc Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé
US11051532B2 (en) * 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
ES3040791T3 (en) 2020-12-18 2025-11-05 Richter Gedeon Nyrt Methods for the purification of refolded fc-peptide fusion protein

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Cited By (7)

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Publication number Priority date Publication date Assignee Title
KR20190037247A (ko) * 2016-07-22 2019-04-05 암젠 인크 Fc-함유 단백질의 정제 방법
JP2019528255A (ja) * 2016-07-22 2019-10-10 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
JP7092744B2 (ja) 2016-07-22 2022-06-28 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
KR102435801B1 (ko) * 2016-07-22 2022-08-25 암젠 인크 Fc-함유 단백질의 정제 방법
KR20220120716A (ko) * 2016-07-22 2022-08-30 암젠 인크 Fc-함유 단백질의 정제 방법
KR102579850B1 (ko) * 2016-07-22 2023-09-18 암젠 인크 Fc-함유 단백질의 정제 방법
JP2022501357A (ja) * 2018-09-21 2022-01-06 テネオバイオ， インコーポレイテッド ヘテロ二量体多重特異性抗体を精製するための方法

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FI3660032T3 (fi) 2025-12-18
EP3660032A1 (en) 2020-06-03
LT3660032T (lt) 2026-02-25
EP2445924B1 (en) 2021-03-03
ES2862580T3 (es) 2021-10-07
US20230016430A1 (en) 2023-01-19
WO2010151688A3 (en) 2011-05-19
US20100331526A1 (en) 2010-12-30
US12312381B2 (en) 2025-05-27
AU2010266093B2 (en) 2013-06-27
US20150361130A1 (en) 2015-12-17
US8940878B2 (en) 2015-01-27
US20200361986A1 (en) 2020-11-19
US9643997B2 (en) 2017-05-09
DK3660032T3 (da) 2025-12-22
ES3056593T3 (en) 2026-02-23
EP2445924B2 (en) 2023-12-13
ES2862580T5 (es) 2024-06-13
MX2011013417A (es) 2012-03-29
CA2765154A1 (en) 2010-12-29
WO2010151688A2 (en) 2010-12-29
EP3660032B1 (en) 2025-10-22
US11407784B2 (en) 2022-08-09
SI3660032T1 (sl) 2026-02-27
AU2010266093A1 (en) 2012-01-19
US10577392B2 (en) 2020-03-03
EP2445924A2 (en) 2012-05-02
US20180037603A1 (en) 2018-02-08

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