ES2862580T5 - Procesos de purificación por captura para proteínas expresadas en un sistema no de mamífero - Google Patents

Procesos de purificación por captura para proteínas expresadas en un sistema no de mamífero Download PDF

Info

Publication number
ES2862580T5
ES2862580T5 ES10729997T ES10729997T ES2862580T5 ES 2862580 T5 ES2862580 T5 ES 2862580T5 ES 10729997 T ES10729997 T ES 10729997T ES 10729997 T ES10729997 T ES 10729997T ES 2862580 T5 ES2862580 T5 ES 2862580T5
Authority
ES
Spain
Prior art keywords
protein
separation matrix
solution
refolding
native
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10729997T
Other languages
English (en)
Spanish (es)
Other versions
ES2862580T3 (es
Inventor
Joseph Shultz
Roger Hart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43242573&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2862580(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of ES2862580T3 publication Critical patent/ES2862580T3/es
Application granted granted Critical
Publication of ES2862580T5 publication Critical patent/ES2862580T5/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
ES10729997T 2009-06-25 2010-06-24 Procesos de purificación por captura para proteínas expresadas en un sistema no de mamífero Active ES2862580T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22047709P 2009-06-25 2009-06-25
PCT/US2010/039854 WO2010151688A2 (en) 2009-06-25 2010-06-24 Capture purification processes for proteins expressed in a non-mammalian system

Publications (2)

Publication Number Publication Date
ES2862580T3 ES2862580T3 (es) 2021-10-07
ES2862580T5 true ES2862580T5 (es) 2024-06-13

Family

ID=43242573

Family Applications (2)

Application Number Title Priority Date Filing Date
ES10729997T Active ES2862580T5 (es) 2009-06-25 2010-06-24 Procesos de purificación por captura para proteínas expresadas en un sistema no de mamífero
ES19209054T Active ES3056593T3 (en) 2009-06-25 2010-06-24 Capture purification processes for proteins expressed in a non-mammalian system

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES19209054T Active ES3056593T3 (en) 2009-06-25 2010-06-24 Capture purification processes for proteins expressed in a non-mammalian system

Country Status (12)

Country Link
US (5) US8940878B2 (https=)
EP (2) EP2445924B2 (https=)
JP (1) JP2012531428A (https=)
AU (1) AU2010266093B2 (https=)
CA (1) CA2765154A1 (https=)
DK (1) DK3660032T3 (https=)
ES (2) ES2862580T5 (https=)
FI (1) FI3660032T3 (https=)
LT (1) LT3660032T (https=)
MX (1) MX2011013417A (https=)
SI (1) SI3660032T1 (https=)
WO (1) WO2010151688A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3366692A1 (en) 2009-06-22 2018-08-29 Amgen, Inc Refolding proteins using a chemically controlled redox state
AU2010266093B2 (en) 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
EP2542574B1 (en) 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
GB201506870D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
KR102579850B1 (ko) * 2016-07-22 2023-09-18 암젠 인크 Fc-함유 단백질의 정제 방법
MA49962A (fr) 2017-08-22 2020-07-01 Biogen Ma Inc Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé
US11051532B2 (en) * 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
SG11202102713TA (en) * 2018-09-21 2021-04-29 Teneobio Inc Methods for purifying heterodimeric, multispecific antibodies
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
ES3040791T3 (en) 2020-12-18 2025-11-05 Richter Gedeon Nyrt Methods for the purification of refolded fc-peptide fusion protein

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
EP0122080B1 (en) 1983-03-25 1989-09-13 Celltech Limited A process for the production of a protein
US4468454A (en) 1983-06-10 1984-08-28 E. I. Du Pont De Nemours And Company Antifoggant process
US4572798A (en) 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
GB8508340D0 (en) 1985-03-29 1985-05-09 Creighton T E Production of protein
US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
IL86090A (en) 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
CA1329119C (en) 1988-03-29 1994-05-03 Milton David Goldenberg Cytotoxic therapy
KR0133561B1 (ko) 1988-05-13 1998-04-21 스티븐 엠. 오드레 과립구 군체 자극인자의 정제방법
GB8927546D0 (en) 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
US5986070A (en) 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
AU664021B2 (en) 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
WO1993018136A1 (en) 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
US5663304A (en) 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
EP0657466B1 (en) 1993-11-10 1997-01-15 Pfizer Inc. A process for refolding of (pro-)chymosin, comprising recycling of urea
US5466377A (en) * 1994-01-19 1995-11-14 Grandics; Peter Chromatography media and their uses
IL113497A (en) 1994-04-28 2001-01-11 Amgen Inc Method for controlling metallophosphate precipitation in high cell density fermentations
AU2603995A (en) 1994-05-25 1995-12-18 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
AU6028396A (en) 1995-06-07 1996-12-30 Amgen, Inc. Ob protein compositions and method
AU729540B2 (en) * 1996-01-23 2001-02-01 Ortho-Mcneil Pharmaceutical, Inc. Methods for purification and use of erythropoietin binding protein
US5935824A (en) 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US20020052026A1 (en) 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6180391B1 (en) 1998-01-28 2001-01-30 Amgen Inc. Highly efficient controlled expression of exogenous genes in e. coli
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
WO1999060120A2 (en) 1998-05-19 1999-11-25 Avidex Limited Soluble t cell receptor
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
ATE313561T1 (de) * 1999-07-21 2006-01-15 Amgen Inc Vgf polypeptide und verfahren zur behandlung von vgf-erkrankungen
US6808902B1 (en) 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
CA2421588C (en) * 2000-09-05 2010-01-26 Amgen Inc. Tnf receptor-like molecules and uses thereof
US20070111944A1 (en) * 2001-02-16 2007-05-17 Scrofani Sergio D B Purification of vascular endothelial growth factor-B
IL157446A0 (en) 2001-02-23 2004-03-28 Immunex Corp Immunex Corp Increased recovery of active proteins
US6972327B1 (en) * 2001-05-08 2005-12-06 Immunex Corporation Regeneration of chromatography material
US7138370B2 (en) 2001-10-11 2006-11-21 Amgen Inc. Specific binding agents of human angiopoietin-2
ITMI20012345A1 (it) 2001-11-08 2003-05-08 Internat Ct For Genetic En Gin Procedimento per la produzione di interferone alfa di grado terapeutico
SI21102A (sl) 2001-12-19 2003-06-30 LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
US7067279B1 (en) 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
BR0317538A (pt) 2002-12-20 2005-11-29 Amgen Inc Agente ligante, sequência de polinucleotìdeo, vetor de expressão, célula hospedeira, composição farmacêutica, e, métodos de inibir a atividade de miostatina, de aumentar a massa muscular magra e a razão de massa muscular magra para gordura, de tratar uma doença de emaciação muscular e um distúrbio metabólico relacionado com miostatina em um indivìduo, de detectar e medir miostatina em uma amostra, e, de diagnosticar um distúrbio relacionado com miostatina em um indivìduo
US7083948B1 (en) 2002-12-24 2006-08-01 Immunex Corporation Polypeptide purification reagents and methods for their use
EP1449848A1 (en) 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Method for the production of cystine-knot proteins
GB0304068D0 (en) 2003-02-22 2003-03-26 Avidex Ltd Substances
US7427659B2 (en) 2003-10-24 2008-09-23 Amgen Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
EP1711512B1 (en) 2004-01-30 2012-01-11 Amgen Inc. Process for purifying proteins
AU2005222782B2 (en) 2004-02-04 2010-06-17 Ratiopharm Gmbh Methods of refolding mammalian glycosyltransferases
JP4765934B2 (ja) 2004-03-10 2011-09-07 大日本印刷株式会社 血管細胞培養用パターニング基板
US20050209441A1 (en) 2004-03-22 2005-09-22 Lile Jackson D Process for promoting proper folding of human serum albumin using a human serum albumin ligand
CA2577690C (en) 2004-08-19 2013-08-06 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
FR2874318B1 (fr) 2004-08-19 2006-11-24 Oreal Utilisation en cosmetique de composes polysaccharidiques amphoteres a chaine(s) polymerique(s) cationique(s)
DE102004041639A1 (de) * 2004-08-27 2006-03-02 Bioceuticals Arzneimittel Ag Verfahren zur Gewinnung von biologisch aktivem humanen G-CSF aus Inclusion Bodies
WO2006036834A2 (en) 2004-09-24 2006-04-06 Amgen Inc. MODIFIED Fc MOLECULES
US7435804B2 (en) 2004-10-19 2008-10-14 Phage Biotechnology, Inc. Method for obtaining single chain antibodies to human interferon α2b
AU2005299716B2 (en) 2004-10-22 2012-09-06 Amgen Inc. Methods for refolding of recombinant antibodies
AU2005314438B2 (en) 2004-12-10 2012-03-29 Zymogenetics, Inc. FGF18 production in prokaryotic hosts
JP2008528039A (ja) 2005-01-28 2008-07-31 ザイモジェネティクス，インコーポレイティド Ｉｌ−３１の均質調製物
WO2006097944A2 (en) 2005-03-17 2006-09-21 Zenotech Laboratories Limited Process for the purification of recombinant granulocyte-colony stimulating factor
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
US8008453B2 (en) 2005-08-12 2011-08-30 Amgen Inc. Modified Fc molecules
US7735525B2 (en) 2005-11-14 2010-06-15 Amgen Inc. Thermally insulated apparatus for liquid chromatographic analysis
WO2007075283A2 (en) 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
BRPI0621124A2 (pt) 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
US7790862B2 (en) 2006-06-13 2010-09-07 Zymogenetics, Inc. IL-17 and IL-23 antagonists and methods of using the same
EP1845103B1 (en) 2006-04-10 2015-02-25 Boehringer Ingelheim RCV GmbH & Co KG Method for refolding a protein
AR062069A1 (es) 2006-07-14 2008-10-15 Genentech Inc Replegado de proteinas recombinantes
EP2049821A4 (en) 2006-07-21 2013-09-04 Amgen Inc Rupture valve
WO2008097829A2 (en) 2007-02-02 2008-08-14 Neose Technologies, Inc. Large scale production of eukaryotic n-acetylglucosaminyltransferase i in bacteria
TW201307390A (zh) 2007-02-02 2013-02-16 Amgen Inc 海帕西啶（ｈｅｐｃｉｄｉｎ）、海帕西啶拮抗劑及使用方法
WO2008096370A2 (en) 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
WO2008100578A2 (en) 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation
JP2010536341A (ja) * 2007-08-15 2010-12-02 アムニクス， インコーポレイテッド 生物学的に活性なポリペプチドの特性を改変するための組成物および方法
CN102015770A (zh) 2008-02-29 2011-04-13 特拉维夫大学拉莫特有限公司 免疫球蛋白组合物和生产其的方法
BRPI0914251B1 (pt) 2008-06-25 2022-07-19 Novartis Ag Imunoligante recombinante, seu fragmento de ligação ao antígeno, uso dos mesmos, e composição
EP3366692A1 (en) 2009-06-22 2018-08-29 Amgen, Inc Refolding proteins using a chemically controlled redox state
AU2010266093B2 (en) 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
TWI582408B (zh) 2011-08-29 2017-05-11 安美基公司 用於非破壞性檢測－流體中未溶解粒子之方法及裝置
US8945872B2 (en) 2013-01-25 2015-02-03 Warsaw Orthopedic, Inc. Methods of purifying human recombinant growth and differentiation factor-5 (rhGDF-5) protein
US20160038588A1 (en) 2013-03-15 2016-02-11 Amgen Inc. Myostatin Antagonism in Human Subjects
US9632095B2 (en) 2014-12-01 2017-04-25 University Of Delaware Device and method for determining reaction kinetics
US9704239B1 (en) 2016-09-02 2017-07-11 Amgen Inc. Video trigger synchronization for improved particle detection in a vessel

Also Published As

Publication number Publication date
FI3660032T3 (fi) 2025-12-18
EP3660032A1 (en) 2020-06-03
LT3660032T (lt) 2026-02-25
EP2445924B1 (en) 2021-03-03
ES2862580T3 (es) 2021-10-07
US20230016430A1 (en) 2023-01-19
WO2010151688A3 (en) 2011-05-19
US20100331526A1 (en) 2010-12-30
US12312381B2 (en) 2025-05-27
AU2010266093B2 (en) 2013-06-27
US20150361130A1 (en) 2015-12-17
US8940878B2 (en) 2015-01-27
US20200361986A1 (en) 2020-11-19
US9643997B2 (en) 2017-05-09
DK3660032T3 (da) 2025-12-22
ES3056593T3 (en) 2026-02-23
EP2445924B2 (en) 2023-12-13
MX2011013417A (es) 2012-03-29
CA2765154A1 (en) 2010-12-29
WO2010151688A2 (en) 2010-12-29
EP3660032B1 (en) 2025-10-22
US11407784B2 (en) 2022-08-09
JP2012531428A (ja) 2012-12-10
SI3660032T1 (sl) 2026-02-27
AU2010266093A1 (en) 2012-01-19
US10577392B2 (en) 2020-03-03
EP2445924A2 (en) 2012-05-02
US20180037603A1 (en) 2018-02-08

Similar Documents

Publication Publication Date Title
ES2862580T5 (es) Procesos de purificación por captura para proteínas expresadas en un sistema no de mamífero
JP6385374B2 (ja) タンパク質製剤からエンドトキシンを除去するための物質および方法
US20100168395A1 (en) Novel polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin
CA2751000A1 (en) Methods of purifying small modular immunopharmaceutical proteins
ES2319844B1 (es) Procedimiento de separacion de proteinas recombinantes en sistemas acuosos de dos fases.
EP2773439A1 (en) Chromatography process for resolving heterogeneous antibody aggregates
Clemmitt et al. Immobilised metal affinity chromatography of β-galactosidase from unclarified Escherichia coli homogenates using expanded bed adsorption
TW201617360A (zh) 用於純化蛋白質之方法及試劑
CA2467048C (en) Method of protein purification
ES2343023T3 (es) Procedimientos para purificar proteinas altamente anionicas.
Barredo et al. Protocol for bevacizumab purification using Ac-PHQGQHIGVSK-agarose