JP2012513386A - 核酸の精製方法 - Google Patents

核酸の精製方法 Download PDF

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JP2012513386A
JP2012513386A JP2011541520A JP2011541520A JP2012513386A JP 2012513386 A JP2012513386 A JP 2012513386A JP 2011541520 A JP2011541520 A JP 2011541520A JP 2011541520 A JP2011541520 A JP 2011541520A JP 2012513386 A JP2012513386 A JP 2012513386A
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nucleic acid
support
binding
propyl
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クリストフ エルバッハー，
ローラント ファビス，
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キアゲン ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
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Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008063001.2 2008-12-23
DE102008063001A DE102008063001A1 (de) 2008-12-23 2008-12-23 Nukleinsäureaufreinigungsverfahren
PCT/EP2009/067909 WO2010072834A1 (de) 2008-12-23 2009-12-23 Nukleinsäureaufreinigungsverfahren

Related Child Applications (1)

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JP2016012103A Division JP6072945B2 (ja) 2008-12-23 2016-01-26 核酸の精製方法

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JP2012513386A true JP2012513386A (ja) 2012-06-14

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JP2016012103A Active JP6072945B2 (ja) 2008-12-23 2016-01-26 核酸の精製方法

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US (1) US9102935B2 (enExample)
EP (1) EP2382315B1 (enExample)
JP (2) JP2012513386A (enExample)
CN (1) CN102264902B (enExample)
AU (1) AU2009332900B2 (enExample)
DE (1) DE102008063001A1 (enExample)
WO (1) WO2010072834A1 (enExample)

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WO2019131760A1 (ja) * 2017-12-27 2019-07-04 東レ株式会社 核酸の回収方法

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DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
WO2013045434A1 (en) 2011-09-26 2013-04-04 Qiagen Gmbh Methods for separating nucleic acids by size
CN113388605A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
US9340828B2 (en) * 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
SG11201407808XA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Chromatographic Purification Of Polynucleotides With Negatively Charged Particles
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
JP6255034B2 (ja) 2012-12-11 2017-12-27 キアゲン ゲーエムベーハー シリカ粒子の調製
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
EP3578663A1 (en) 2013-03-15 2019-12-11 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
BR112017004469A2 (pt) * 2014-09-10 2017-12-05 Quantumdx Group Ltd material sorvente para separar bio-macromoléculas
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
US11118173B2 (en) 2015-03-20 2021-09-14 Toray Industries, Inc. Method of collecting a nucleic acid(s)
EP3307886B1 (en) 2015-06-10 2021-03-24 Qiagen GmbH Method for isolating extracellular nucleic acids using anion exchange particles
WO2017162518A1 (en) 2016-03-19 2017-09-28 Qiagen Gmbh Stabilization of rna
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
CN108102881A (zh) * 2018-02-05 2018-06-01 中国科学院苏州纳米技术与纳米仿生研究所 具有微结构阵列的微流控芯片及核酸固相纯化提取方法
CN108795929A (zh) * 2018-07-06 2018-11-13 大连元和健路医学检验实验室有限公司 一种dna分子筛柱子的制备方法
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
WO2020202952A1 (ja) 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
US20230028205A1 (en) 2019-12-16 2023-01-26 Qiagen Gmbh Enrichment method
US20240287498A1 (en) 2021-06-17 2024-08-29 Qiagen Gmbh Method for isolating non-vesicular mirna
US20250011845A1 (en) 2021-11-12 2025-01-09 Solventum Intellectual Properties Company Method of Processing Polynucleic Acids
CN115537416A (zh) * 2022-09-29 2022-12-30 杭州联川生物技术股份有限公司 一种纯化寡核苷酸的方法和试剂盒

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS646755A (en) * 1987-01-21 1989-01-11 Du Pont Chromatography support body for oligonucleotide separation
JPH09157282A (ja) * 1995-12-04 1997-06-17 Japan Synthetic Rubber Co Ltd 核酸吸着剤
JPH10502052A (ja) * 1994-04-08 1998-02-24 ハイブライドン インコーポレイテッド 陰イオン交換クロマトグラフィーを用いるオリゴデオキシヌクレオチド・ホスホロチエオエートの精製
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JP2002543979A (ja) * 1999-05-14 2002-12-24 プロメガ コーポレイション ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法
JP2004521881A (ja) * 2000-12-14 2004-07-22 ディーエヌエー リサーチ イノヴェイションズ リミテッド 核酸の分離

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US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
KR20010032806A (ko) 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
KR100714988B1 (ko) 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules
WO2008035991A2 (en) 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS646755A (en) * 1987-01-21 1989-01-11 Du Pont Chromatography support body for oligonucleotide separation
JPH10502052A (ja) * 1994-04-08 1998-02-24 ハイブライドン インコーポレイテッド 陰イオン交換クロマトグラフィーを用いるオリゴデオキシヌクレオチド・ホスホロチエオエートの精製
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JPH09157282A (ja) * 1995-12-04 1997-06-17 Japan Synthetic Rubber Co Ltd 核酸吸着剤
JP2002543979A (ja) * 1999-05-14 2002-12-24 プロメガ コーポレイション ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法
JP2004521881A (ja) * 2000-12-14 2004-07-22 ディーエヌエー リサーチ イノヴェイションズ リミテッド 核酸の分離
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019131760A1 (ja) * 2017-12-27 2019-07-04 東レ株式会社 核酸の回収方法
US11685915B2 (en) 2017-12-27 2023-06-27 Toray Industries, Inc. Method of collecting nucleic acid

Also Published As

Publication number Publication date
JP6072945B2 (ja) 2017-02-01
US20110319506A1 (en) 2011-12-29
CN102264902B (zh) 2018-06-01
CN102264902A (zh) 2011-11-30
AU2009332900A1 (en) 2010-07-01
WO2010072834A1 (de) 2010-07-01
DE102008063001A1 (de) 2010-06-24
AU2009332900B2 (en) 2014-05-29
EP2382315A1 (de) 2011-11-02
JP2016135784A (ja) 2016-07-28
US9102935B2 (en) 2015-08-11
EP2382315B1 (de) 2015-09-23

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