AU2009332900B2 - Nucleic acid purification method - Google Patents

Nucleic acid purification method Download PDF

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Publication number
AU2009332900B2
AU2009332900B2 AU2009332900A AU2009332900A AU2009332900B2 AU 2009332900 B2 AU2009332900 B2 AU 2009332900B2 AU 2009332900 A AU2009332900 A AU 2009332900A AU 2009332900 A AU2009332900 A AU 2009332900A AU 2009332900 B2 AU2009332900 B2 AU 2009332900B2
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Australia
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groups
binding
group
support material
dimethylamino
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AU2009332900A
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AU2009332900A1 (en
Inventor
Christoph Erbacher
Roland Fabis
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
AU2009332900A 2008-12-23 2009-12-23 Nucleic acid purification method Active AU2009332900B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008063001A DE102008063001A1 (de) 2008-12-23 2008-12-23 Nukleinsäureaufreinigungsverfahren
DE102008063001.2 2008-12-23
PCT/EP2009/067909 WO2010072834A1 (de) 2008-12-23 2009-12-23 Nukleinsäureaufreinigungsverfahren

Publications (2)

Publication Number Publication Date
AU2009332900A1 AU2009332900A1 (en) 2010-07-01
AU2009332900B2 true AU2009332900B2 (en) 2014-05-29

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ID=42078045

Family Applications (1)

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AU2009332900A Active AU2009332900B2 (en) 2008-12-23 2009-12-23 Nucleic acid purification method

Country Status (7)

Country Link
US (1) US9102935B2 (enExample)
EP (1) EP2382315B1 (enExample)
JP (2) JP2012513386A (enExample)
CN (1) CN102264902B (enExample)
AU (1) AU2009332900B2 (enExample)
DE (1) DE102008063001A1 (enExample)
WO (1) WO2010072834A1 (enExample)

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DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5899731B2 (ja) * 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
WO2013045434A1 (en) 2011-09-26 2013-04-04 Qiagen Gmbh Methods for separating nucleic acids by size
CN113355321A (zh) 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
US9340828B2 (en) 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
KR20150023331A (ko) * 2012-05-31 2015-03-05 에이전시 포 사이언스, 테크놀로지 앤드 리서치 음으로 대전된 입자들로의 폴리뉴클레오티드들의 크로마토그래피 정제
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
US9938520B2 (en) 2012-12-11 2018-04-10 Qiagen Gmbh Preparation of silica particles
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
EP2983804A4 (en) * 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
CN106604992B (zh) * 2014-09-10 2021-06-18 康特姆斯集团有限公司 用于分离生物大分子的吸附剂材料
US10319502B2 (en) 2014-10-23 2019-06-11 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
KR102488291B1 (ko) * 2015-03-20 2023-01-13 도레이 카부시키가이샤 핵산의 회수 방법
EP3307886B1 (en) 2015-06-10 2021-03-24 Qiagen GmbH Method for isolating extracellular nucleic acids using anion exchange particles
JP7163186B2 (ja) 2016-03-19 2022-10-31 キアゲン ゲーエムベーハー Ｒｎａの安定化
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
CN111542605A (zh) 2017-12-27 2020-08-14 东丽株式会社 核酸的回收方法
CN108102881A (zh) * 2018-02-05 2018-06-01 中国科学院苏州纳米技术与纳米仿生研究所 具有微结构阵列的微流控芯片及核酸固相纯化提取方法
CN108795929A (zh) * 2018-07-06 2018-11-13 大连元和健路医学检验实验室有限公司 一种dna分子筛柱子的制备方法
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
WO2020202952A1 (ja) * 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
EP4077659A1 (en) 2019-12-16 2022-10-26 QIAGEN GmbH Enrichment method
WO2022263500A1 (en) 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna
CN118339287A (zh) 2021-11-12 2024-07-12 舒万诺知识产权公司 加工多核酸的方法
CN115537416A (zh) * 2022-09-29 2022-12-30 杭州联川生物技术股份有限公司 一种纯化寡核苷酸的方法和试剂盒

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2002048164A2 (en) * 2000-12-14 2002-06-20 Dna Research Innovations Limited Isolation of nucleic acids
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules

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US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
CN1150431A (zh) * 1994-04-08 1997-05-21 海布里顿公司 用阴离子交换层析法纯化寡脱氧核苷酸硫代磷酸酯
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
JP3689896B2 (ja) * 1995-12-04 2005-08-31 Ｊｓｒ株式会社 核酸吸着剤
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
EP1036082B1 (en) 1997-12-06 2002-05-29 DNA Research Innovations Limited Isolation of nucleic acids
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法
KR100714988B1 (ko) * 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2002048164A2 (en) * 2000-12-14 2002-06-20 Dna Research Innovations Limited Isolation of nucleic acids
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules

Also Published As

Publication number Publication date
US20110319506A1 (en) 2011-12-29
JP2012513386A (ja) 2012-06-14
WO2010072834A1 (de) 2010-07-01
JP6072945B2 (ja) 2017-02-01
CN102264902B (zh) 2018-06-01
DE102008063001A1 (de) 2010-06-24
CN102264902A (zh) 2011-11-30
AU2009332900A1 (en) 2010-07-01
EP2382315B1 (de) 2015-09-23
JP2016135784A (ja) 2016-07-28
EP2382315A1 (de) 2011-11-02
US9102935B2 (en) 2015-08-11

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