CN102264902B - 核酸纯化方法 - Google Patents

核酸纯化方法 Download PDF

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Publication number
CN102264902B
CN102264902B CN200980152958.XA CN200980152958A CN102264902B CN 102264902 B CN102264902 B CN 102264902B CN 200980152958 A CN200980152958 A CN 200980152958A CN 102264902 B CN102264902 B CN 102264902B
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nucleic acid
support material
groups
diethylamino
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Chinese (zh)
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CN102264902A (zh
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C·埃尔巴赫
R·法比斯
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN200980152958.XA 2008-12-23 2009-12-23 核酸纯化方法 Active CN102264902B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008063001.2 2008-12-23
DE102008063001A DE102008063001A1 (de) 2008-12-23 2008-12-23 Nukleinsäureaufreinigungsverfahren
PCT/EP2009/067909 WO2010072834A1 (de) 2008-12-23 2009-12-23 Nukleinsäureaufreinigungsverfahren

Publications (2)

Publication Number Publication Date
CN102264902A CN102264902A (zh) 2011-11-30
CN102264902B true CN102264902B (zh) 2018-06-01

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CN200980152958.XA Active CN102264902B (zh) 2008-12-23 2009-12-23 核酸纯化方法

Country Status (7)

Country Link
US (1) US9102935B2 (enExample)
EP (1) EP2382315B1 (enExample)
JP (2) JP2012513386A (enExample)
CN (1) CN102264902B (enExample)
AU (1) AU2009332900B2 (enExample)
DE (1) DE102008063001A1 (enExample)
WO (1) WO2010072834A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
WO2013045434A1 (en) 2011-09-26 2013-04-04 Qiagen Gmbh Methods for separating nucleic acids by size
CN113388605A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
US9340828B2 (en) * 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
SG11201407808XA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Chromatographic Purification Of Polynucleotides With Negatively Charged Particles
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
JP6255034B2 (ja) 2012-12-11 2017-12-27 キアゲン ゲーエムベーハー シリカ粒子の調製
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
EP3578663A1 (en) 2013-03-15 2019-12-11 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
BR112017004469A2 (pt) * 2014-09-10 2017-12-05 Quantumdx Group Ltd material sorvente para separar bio-macromoléculas
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
US11118173B2 (en) 2015-03-20 2021-09-14 Toray Industries, Inc. Method of collecting a nucleic acid(s)
EP3307886B1 (en) 2015-06-10 2021-03-24 Qiagen GmbH Method for isolating extracellular nucleic acids using anion exchange particles
WO2017162518A1 (en) 2016-03-19 2017-09-28 Qiagen Gmbh Stabilization of rna
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
CA3086996A1 (en) * 2017-12-27 2019-07-04 Toray Industries, Inc. Method for collecting nucleic acid
CN108102881A (zh) * 2018-02-05 2018-06-01 中国科学院苏州纳米技术与纳米仿生研究所 具有微结构阵列的微流控芯片及核酸固相纯化提取方法
CN108795929A (zh) * 2018-07-06 2018-11-13 大连元和健路医学检验实验室有限公司 一种dna分子筛柱子的制备方法
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
WO2020202952A1 (ja) 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
US20230028205A1 (en) 2019-12-16 2023-01-26 Qiagen Gmbh Enrichment method
US20240287498A1 (en) 2021-06-17 2024-08-29 Qiagen Gmbh Method for isolating non-vesicular mirna
US20250011845A1 (en) 2021-11-12 2025-01-09 Solventum Intellectual Properties Company Method of Processing Polynucleic Acids
CN115537416A (zh) * 2022-09-29 2022-12-30 杭州联川生物技术股份有限公司 一种纯化寡核苷酸的方法和试剂盒

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
JPH10502052A (ja) * 1994-04-08 1998-02-24 ハイブライドン インコーポレイテッド 陰イオン交換クロマトグラフィーを用いるオリゴデオキシヌクレオチド・ホスホロチエオエートの精製
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
JP3689896B2 (ja) * 1995-12-04 2005-08-31 Ｊｓｒ株式会社 核酸吸着剤
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
KR20010032806A (ko) 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法
KR100714988B1 (ko) 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method

Also Published As

Publication number Publication date
JP6072945B2 (ja) 2017-02-01
US20110319506A1 (en) 2011-12-29
CN102264902A (zh) 2011-11-30
AU2009332900A1 (en) 2010-07-01
WO2010072834A1 (de) 2010-07-01
DE102008063001A1 (de) 2010-06-24
AU2009332900B2 (en) 2014-05-29
JP2012513386A (ja) 2012-06-14
EP2382315A1 (de) 2011-11-02
JP2016135784A (ja) 2016-07-28
US9102935B2 (en) 2015-08-11
EP2382315B1 (de) 2015-09-23

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