CN102264902B - 核酸纯化方法 - Google Patents

核酸纯化方法 Download PDF

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Publication number
CN102264902B
CN102264902B CN200980152958.XA CN200980152958A CN102264902B CN 102264902 B CN102264902 B CN 102264902B CN 200980152958 A CN200980152958 A CN 200980152958A CN 102264902 B CN102264902 B CN 102264902B
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nucleic acid
support material
groups
diethylamino
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Chinese (zh)
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CN102264902A (zh
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C·埃尔巴赫
R·法比斯
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN200980152958.XA 2008-12-23 2009-12-23 核酸纯化方法 Active CN102264902B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008063001.2 2008-12-23
DE102008063001A DE102008063001A1 (de) 2008-12-23 2008-12-23 Nukleinsäureaufreinigungsverfahren
PCT/EP2009/067909 WO2010072834A1 (de) 2008-12-23 2009-12-23 Nukleinsäureaufreinigungsverfahren

Publications (2)

Publication Number Publication Date
CN102264902A CN102264902A (zh) 2011-11-30
CN102264902B true CN102264902B (zh) 2018-06-01

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CN200980152958.XA Active CN102264902B (zh) 2008-12-23 2009-12-23 核酸纯化方法

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US (1) US9102935B2 (enExample)
EP (1) EP2382315B1 (enExample)
JP (2) JP2012513386A (enExample)
CN (1) CN102264902B (enExample)
AU (1) AU2009332900B2 (enExample)
DE (1) DE102008063001A1 (enExample)
WO (1) WO2010072834A1 (enExample)

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DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5899731B2 (ja) * 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
US20140243216A1 (en) 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
CN113388605A (zh) 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
EP2771462A4 (en) * 2011-10-27 2015-04-08 Ge Healthcare Bio Sciences Ab PURIFICATION OF NUCLEIC ACIDS
CN104470606B (zh) * 2012-05-31 2017-03-22 新加坡科技研究局 用带负电荷颗粒对多核苷酸的色谱纯化
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
JP6255034B2 (ja) 2012-12-11 2017-12-27 キアゲン ゲーエムベーハー シリカ粒子の調製
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
US10792640B2 (en) * 2014-09-10 2020-10-06 Quantumdx Group Limited Sorbent material for separating bio-macromolecules
US10319502B2 (en) 2014-10-23 2019-06-11 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
US11118173B2 (en) 2015-03-20 2021-09-14 Toray Industries, Inc. Method of collecting a nucleic acid(s)
EP3885438A1 (en) 2015-06-10 2021-09-29 QIAGEN GmbH Method for isolating extracellular nucleic acids using anion exchange particles
WO2017162518A1 (en) 2016-03-19 2017-09-28 Qiagen Gmbh Stabilization of rna
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
KR102689348B1 (ko) 2017-12-27 2024-07-30 도레이 카부시키가이샤 핵산의 회수 방법
CN108102881A (zh) * 2018-02-05 2018-06-01 中国科学院苏州纳米技术与纳米仿生研究所 具有微结构阵列的微流控芯片及核酸固相纯化提取方法
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
CN108795929A (zh) * 2018-07-06 2018-11-13 大连元和健路医学检验实验室有限公司 一种dna分子筛柱子的制备方法
WO2020202952A1 (ja) * 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
WO2021122846A1 (en) 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
EP4355873A1 (en) 2021-06-17 2024-04-24 QIAGEN GmbH Method for isolating non-vesicular mirna
CN118339287A (zh) 2021-11-12 2024-07-12 舒万诺知识产权公司 加工多核酸的方法
CN115537416A (zh) * 2022-09-29 2022-12-30 杭州联川生物技术股份有限公司 一种纯化寡核苷酸的方法和试剂盒

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules

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US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
WO1995027718A2 (en) * 1994-04-08 1995-10-19 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
JP3689896B2 (ja) * 1995-12-04 2005-08-31 Ｊｓｒ株式会社 核酸吸着剤
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
ES2177093T3 (es) 1997-12-06 2002-12-01 Dna Res Innovations Ltd Aislamiento de acidos nucleicos.
JP2003104996A (ja) * 2001-09-28 2003-04-09 Hitachi Maxell Ltd 核酸結合用磁性担体およびその製造方法
KR100714988B1 (ko) * 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069872A2 (en) * 1999-05-14 2000-11-23 Promega Corporation pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS
WO2008097342A2 (en) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions and methods for isolation of biological molecules
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method

Also Published As

Publication number Publication date
US9102935B2 (en) 2015-08-11
EP2382315A1 (de) 2011-11-02
US20110319506A1 (en) 2011-12-29
AU2009332900A1 (en) 2010-07-01
EP2382315B1 (de) 2015-09-23
JP2016135784A (ja) 2016-07-28
JP2012513386A (ja) 2012-06-14
DE102008063001A1 (de) 2010-06-24
AU2009332900B2 (en) 2014-05-29
WO2010072834A1 (de) 2010-07-01
JP6072945B2 (ja) 2017-02-01
CN102264902A (zh) 2011-11-30

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