JP2007500008A5 - - Google Patents

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JP2007500008A5
JP2007500008A5 JP2006521947A JP2006521947A JP2007500008A5 JP 2007500008 A5 JP2007500008 A5 JP 2007500008A5 JP 2006521947 A JP2006521947 A JP 2006521947A JP 2006521947 A JP2006521947 A JP 2006521947A JP 2007500008 A5 JP2007500008 A5 JP 2007500008A5
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solution
lysate
small rna
alcohol
rna molecules
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JP2006521947A
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JP2007500008A (ja
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Priority claimed from US10/667,126 external-priority patent/US20050059024A1/en
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JP2006521947A 2003-07-25 2004-07-26 小型ｒｎａ分子を単離するための方法および組成物 Pending JP2007500008A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US49032503P 2003-07-25 2003-07-25
US10/667,126 US20050059024A1 (en) 2003-07-25 2003-09-19 Methods and compositions for isolating small RNA molecules
PCT/US2004/023850 WO2005012523A1 (en) 2003-07-25 2004-07-26 Methods and compositions for isolating small rna molecules

Publications (2)

Publication Number Publication Date
JP2007500008A JP2007500008A (ja) 2007-01-11
JP2007500008A5 true JP2007500008A5 (https=) 2007-08-23

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JP2006521947A Pending JP2007500008A (ja) 2003-07-25 2004-07-26 小型ｒｎａ分子を単離するための方法および組成物

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US (5) US20050059024A1 (https=)
EP (4) EP2298891B2 (https=)
JP (1) JP2007500008A (https=)
ES (2) ES2721631T3 (https=)
WO (1) WO2005012523A1 (https=)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP2216415B2 (en) * 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
JP5697297B2 (ja) 2004-05-14 2015-04-08 ロゼッタ ジノミクス リミテッド マイクロｎａｓおよびその使用
US7795419B2 (en) 2004-05-26 2010-09-14 Rosetta Genomics Ltd. Viral and viral associated miRNAs and uses thereof
EP2290073A3 (en) * 2004-05-28 2011-08-31 Asuragen, Inc. Methods and compositions involving microRNA
EP2302055B1 (en) 2004-11-12 2014-08-27 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
CN101268198A (zh) * 2005-01-21 2008-09-17 纽约血液中心有限公司 用于核酸提取和鉴定的方法
JP2006292367A (ja) * 2005-04-05 2006-10-26 Mitsubishi Rayon Co Ltd ｍｉＲＮＡ検出用マイクロアレイ
WO2006130632A2 (en) 2005-05-31 2006-12-07 Invitrogen Corporation Separation and purification of nucleic acid from paraffin-containing samples
AU2006270063A1 (en) * 2005-07-15 2007-01-25 Applied Biosystems, Llc. Methods for RNA profiling
EP1924708A4 (en) * 2005-09-12 2010-03-10 Applera Corp PREPARATION METHOD FOR PURE MIRNA SAMPLE
CA2626977A1 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
KR100658605B1 (ko) 2006-01-31 2006-12-19 한국생명공학연구원 시아노박테리아로부터 고효율로 ｒｎａ를 추출하는 방법
US20070202511A1 (en) * 2006-02-28 2007-08-30 Sigma-Aldrich Co. Methods and compositions for the rapid isolation of small RNA molecules
US20070207483A1 (en) * 2006-03-02 2007-09-06 Bio-Rad Laboratories, Inc. BUFFERS FOR DETECTION OF mRNA SEPARATED IN A MICROFLUIDIC DEVICE
WO2007103485A2 (en) * 2006-03-08 2007-09-13 Promega Corporation Small rna purification
EP3617321B1 (en) * 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
WO2008036776A2 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
US20080081330A1 (en) * 2006-09-28 2008-04-03 Helicos Biosciences Corporation Method and devices for analyzing small RNA molecules
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
WO2008073915A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Micrornas differentially expressed in leukemia and uses thereof
AU2007333109A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro RNAs
AU2007333107A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-21 regulated genes and pathways as targets for therapeutic intervention
EP2104734A2 (en) * 2006-12-08 2009-09-30 Asuragen, INC. Mir-20 regulated genes and pathways as targets for therapeutic intervention
EP1932913B1 (en) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nucleic acid isolation using polidocanol and derivatives
CN102776173B (zh) * 2006-12-11 2014-10-29 霍夫曼-拉罗奇有限公司 使用聚多卡醇和衍生物的核酸分离
WO2008071384A1 (en) 2006-12-13 2008-06-19 Roche Diagnostics Gmbh Use of tde for the isolation of nucleic acids
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
AU2008261951A1 (en) * 2007-06-08 2008-12-18 Asuragen, Inc. miR-34 regulated genes and pathways as targets for therapeutic intervention
MY157651A (en) * 2007-07-23 2016-07-15 Yiap Beow Chin A method, kit and apparatus for extracting biological materials
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
WO2009036332A1 (en) 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
WO2009052386A1 (en) * 2007-10-18 2009-04-23 Asuragen, Inc. Micrornas differentially expressed in lung diseases and uses thereof
KR101432034B1 (ko) * 2007-11-16 2014-08-21 삼성전자주식회사 코스모트로픽 염을 이용한 고체 지지체에서의 작은 ｒｎａ분리 방법
CN101878304B (zh) * 2007-11-30 2015-01-07 通用电气医疗集团生物科学公司 用于从单个样品中分离基因组dna、rna和蛋白质的方法
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
EP2260110B1 (en) * 2008-02-08 2014-11-12 Asuragen, INC. miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
EP2271757A2 (en) * 2008-03-26 2011-01-12 Asuragen, INC. Compositions and methods related to mir-16 and therapy of prostate cancer
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
WO2009137807A2 (en) * 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US10316352B2 (en) 2008-05-13 2019-06-11 Gen-Probe Incorporated Methods of capturing a target nucleic acid for amplification and detection using an inactivatable target capture oligomer
EP2128169A1 (de) * 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
EP2853601B1 (en) 2008-07-18 2016-09-21 TrovaGene, Inc. Methods for PCR-based detection of "ultra short" nucleic acid sequences
DE102008045705A1 (de) 2008-09-04 2010-04-22 Macherey, Nagel Gmbh & Co. Kg Handelsgesellschaft Verfahren zur Gewinnung von kurzer RNA sowie Kit hierfür
JP2012502632A (ja) * 2008-09-17 2012-02-02 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション スモールｒｎａの単離法
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
WO2010056337A2 (en) 2008-11-12 2010-05-20 Caris Mpi, Inc. Methods and systems of using exosomes for determining phenotypes
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
WO2010120814A1 (en) 2009-04-14 2010-10-21 Prometheus Laboratories Inc. Inflammatory bowel disease prognostics
EP2264168B1 (de) * 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
WO2011060098A1 (en) 2009-11-10 2011-05-19 Prometheus Laboratories Inc. Methods for predicting post-surgery risk associated with ileal pouch-anal anastomosis
CA2782284A1 (en) 2009-11-30 2011-06-03 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Methods and systems for isolating, storing, and analyzing vesicles
US20140148348A1 (en) 2010-01-13 2014-05-29 Christine Kuslich Dectection of gastrointestinal disorders
US8846631B2 (en) 2010-01-14 2014-09-30 Regulus Therapeutics Inc. MicroRNA compositions and methods
WO2011104032A1 (en) 2010-02-26 2011-09-01 Qiagen Gmbh Method for isolating rna from a rna and dna containing sample
EP2542696B1 (en) 2010-03-01 2016-09-28 Caris Life Sciences Switzerland Holdings GmbH Biomarkers for theranostics
JP2013526852A (ja) 2010-04-06 2013-06-27 カリス ライフ サイエンシズ ルクセンブルク ホールディングス 疾患に対する循環バイオマーカー
US20120077198A1 (en) * 2010-07-30 2012-03-29 Ambergen, Inc Compositions And Methods For Cancer Testing
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna
BR112013012265A2 (pt) 2010-11-17 2016-08-02 Asuragen Inc mirnas como biomarcadores para distinguir neoplasmas de tireoide benignos de malignos
WO2012075133A1 (en) 2010-11-30 2012-06-07 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
US9051563B2 (en) * 2011-01-14 2015-06-09 Zymo Research Corporation Nucleic acid purification
WO2012125220A2 (en) * 2011-01-14 2012-09-20 Life Technologies Corporation Methods for isolation, identification, and quantification of mirnas
SG10201600836PA (en) 2011-02-03 2016-03-30 Mirna Therapeutics Inc Synthetic mimics of mir-34
WO2012174293A2 (en) 2011-06-14 2012-12-20 Nestec Sa Methods for identifying inflammatory bowel disease patients with dysplasia or cancer
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
GB2561425B (en) 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
US9206469B2 (en) 2012-07-18 2015-12-08 Zymo Research Corporation Nucleic acid purification
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
WO2014033326A1 (en) 2012-09-03 2014-03-06 Qiagen Gmbh Method for isolating rna including small rna with high yield
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法
US20140287408A1 (en) * 2013-03-13 2014-09-25 Abbott Molecular Inc. Target sequence enrichment
JP6586075B2 (ja) 2013-03-14 2019-10-02 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製方法
EP3828284A1 (en) * 2013-03-15 2021-06-02 Abbott Molecular Inc. One-step procedure for the purification of nucleic acids
US9828600B2 (en) 2013-09-20 2017-11-28 University Of Massachusetts Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs
US9850269B2 (en) 2014-04-25 2017-12-26 Translate Bio, Inc. Methods for purification of messenger RNA
US10954507B2 (en) 2014-07-17 2021-03-23 Qiagen Gmbh Method for isolating RNA with high yield
IL234246A0 (en) * 2014-08-21 2014-11-30 Omrix Biopharmaceuticals Ltd Stabilized thrombin
WO2016057891A1 (en) 2014-10-09 2016-04-14 Promega Corporation Method of isolating nucleic acid
EP3034619B1 (de) * 2014-12-16 2018-10-17 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
US10968443B2 (en) * 2014-12-31 2021-04-06 The Rockefeller University Method of RNA isolation from clinical samples
EP3901259A1 (en) 2015-10-28 2021-10-27 Mirnagreen S.r.l. Methods for extracting bioactive small rnas from plants and mushrooms
US10214739B2 (en) * 2015-10-30 2019-02-26 Axagarius Gmbh & Co. Kg RNA binding solution
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
US10287625B2 (en) * 2016-03-18 2019-05-14 Norgen Biotek Corp. Methods and kits for separating nucleic acids by size
CN107267497B (zh) * 2016-04-08 2018-06-22 北京爱普拜生物技术有限公司 一种通用型总rna提取试剂盒及方法
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
PL3568475T3 (pl) 2017-01-16 2023-06-19 Spectrum Solutions L.L.C. Roztwór do konserwacji kwasu nukleinowego i sposoby zastosowania
KR101885038B1 (ko) * 2017-09-27 2018-08-03 전남대학교산학협력단 바이러스 rna를 추출하는 방법
WO2019063071A1 (en) 2017-09-27 2019-04-04 Qiagen Gmbh METHOD FOR HIGH-PERFORMANCE RNA ISOLATION
US11434480B2 (en) 2017-09-27 2022-09-06 Industry Foundation Of Chonnam National University Viral RNA extracting composition and viral RNA extracting method
WO2019116234A1 (en) 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes
JP7238766B2 (ja) * 2017-12-27 2023-03-14 東レ株式会社 核酸の回収方法
KR102094079B1 (ko) * 2018-07-27 2020-03-27 전남대학교산학협력단 실리카 코팅 자성 비드를 이용한 바이러스 rna 추출용 조성물 및 이를 이용한 바이러스 rna 추출 방법
KR102950141B1 (ko) 2018-08-24 2026-04-07 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
CN109569023B (zh) * 2018-11-29 2021-03-02 杭州立昂科技有限公司 参杂去污剂的干燥水凝胶颗粒及大分子浓缩和比活性提升
SI3896159T1 (sl) * 2020-04-17 2025-08-29 Sartorius Bia Separations D.O.O. Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca
CN111254141B (zh) 2020-04-28 2020-08-04 博奥生物集团有限公司 核酸提取组合物及其应用，含有该核酸提取组合物的试剂、试剂盒
EP4179084A1 (en) 2020-07-07 2023-05-17 Bioecho Life Sciences GmbH Method for isolating nucleic acid
IL299826A (en) 2020-07-15 2023-03-01 Cerebral Therapeutics Inc A medical device with a non-filtered CSF withdrawal path
KR102421648B1 (ko) * 2020-10-08 2022-07-18 주식회사 인바이러스테크 Rna 분해효소가 풍부하게 함유된 시료로부터 핵산을 추출하는 방법
KR102415720B1 (ko) * 2020-10-08 2022-07-01 주식회사 인바이러스테크 폴리페놀 및/또는 다당류가 풍부하게 함유된 시료로부터 핵산을 추출하는 방법
CN112746055A (zh) * 2020-12-30 2021-05-04 华南农业大学 一种牛奶体细胞分离过程优化工艺
EP4134432A1 (en) * 2021-08-11 2023-02-15 Mirnagreen S.r.l. Improved method for small rna purification
US12605537B2 (en) 2022-02-15 2026-04-21 Biogen Ma Inc. Implantable medical device for use with or having recording electrode
CN114517196A (zh) * 2022-02-25 2022-05-20 杭州诺辉健康科技有限公司 一种血浆游离miRNA提取方法及其应用
CN114426970A (zh) * 2022-03-17 2022-05-03 黑龙江省科学院高技术研究院 一种适合杨树rna的提取方法

Family Cites Families (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4283489A (en) * 1977-09-23 1981-08-11 The Regents Of The University Of California Purification of nucleotide sequences suitable for expression in bacteria
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DE3619781A1 (de) 1986-04-17 1987-10-22 Herbert Preuss Florfaserbuerste zur reinigung von textilgeweben
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US5115018A (en) * 1987-08-24 1992-05-19 Allied-Signal Inc. High-impact, styrenic polymer/thermoplastic polymer grafted blends
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
ATE92538T1 (de) 1988-01-21 1993-08-15 Genentech Inc Verstaerkung und nachweis von nukleinsaeuresequenzen.
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
EP0446260B1 (en) 1988-11-21 1994-03-02 Dynal A.S. Nucleic acid probes
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
EP0506574B1 (fr) * 1991-03-29 1995-11-29 Sanofi Protéine à activité de type cytokine, ADN recombinant codant pour cette protéine, cellules et microorganismes transformés
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5260048A (en) 1991-05-08 1993-11-09 Streck Laboratories, Inc. Tissue fixative solution and method
US5196182A (en) 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5480972A (en) 1992-10-30 1996-01-02 The University Of Melbourne Allergenic proteins from Johnson grass pollen
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
CA2170604C (en) 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US6180778B1 (en) * 1994-02-11 2001-01-30 Qiagen Gmbh Process for the separation of double-stranded/single-stranded nucleic acid structures
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
ATE220424T1 (de) 1994-05-28 2002-07-15 Tepnel Medical Ltd Herstellung von nukleinsaeurekopien
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
DE69528706T2 (de) 1994-08-19 2003-06-12 Pe Corp. (Ny), Foster City Gekoppeltes ampflikation- und ligationverfahren
US5935825A (en) 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
US6168918B1 (en) 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
CA2188816C (en) * 1996-04-23 2009-09-22 Takeda Chemical Industries, Ltd. Composition containing .alpha.-fodrin or .alpha.- fodrin fragment protein
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
DE69734263T2 (de) * 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
WO1998012354A1 (en) 1996-09-19 1998-03-26 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
EP0975807B1 (en) * 1997-05-02 2006-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
WO1998059076A1 (en) 1997-06-25 1998-12-30 Promega Corporation Method of isolating rna
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
US6111096A (en) * 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
EP1854890A1 (en) * 1997-12-22 2007-11-14 DSM IP Assets B.V. Expression cloning in filamentous fungi
US6387695B1 (en) 1997-12-23 2002-05-14 Merck & Co., Inc. DNA pharmaceutical formulations comprising citrate or triethanolamine and combinations thereof
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6324479B1 (en) 1998-05-08 2001-11-27 Rosetta Impharmatics, Inc. Methods of determining protein activity levels using gene expression profiles
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6316193B1 (en) 1998-10-06 2001-11-13 Origene Technologies, Inc. Rapid-screen cDNA library panels
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
AU6380001A (en) 2000-03-24 2001-10-03 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
JP3692395B2 (ja) * 2000-09-01 2005-09-07 独立行政法人農業・生物系特定産業技術研究機構 ベクターモノカリオンを用いた紫紋羽病菌に対するゲノムが２本鎖ｒｎａである糸状菌に寄生するウイルスを導入する新規方法
US20020086291A1 (en) 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
US20020139751A1 (en) * 2001-02-20 2002-10-03 Sheng Zhang Microchip electrospray device and column with affinity adsorbents and use of the same
EP1386004A4 (en) 2001-04-05 2005-02-16 Ribozyme Pharm Inc MODULATION OF GENE EXPRESSION ASSOCIATED WITH INFLAMMATORY PROLIFERATION AND GROWTH OF NEURITIES BY METHODS INVOLVING NUCLEIC ACID
US6509175B2 (en) 2001-04-11 2003-01-21 Incyte Genomics, Inc. cDNA libraries and methods for their production
JP2002345462A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 吸着バッファ及び核酸精製方法
US20020197611A1 (en) 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
WO2003046146A2 (en) * 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
AU2003239544A1 (en) * 2002-05-24 2003-12-12 Tularik Inc. Amplification and overexpression of oncogenes
EP1433858A1 (en) 2002-12-27 2004-06-30 Rengo Co., Ltd. Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution
KR100501526B1 (ko) 2003-03-28 2005-07-18 한국기초과학지원연구원 어피니티 칼럼과 디엔에이자임을 이용한 고효율 알엔에이 제조방법
WO2005054466A2 (en) * 2003-07-25 2005-06-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
EP2216415B2 (en) 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
AU2004288132A1 (en) * 2003-08-12 2005-05-19 Massachusetts Institute Of Technology Sample preparation methods and devices
US20060233671A1 (en) 2003-09-19 2006-10-19 Beard Nigel P High density plate filler
EP2290073A3 (en) * 2004-05-28 2011-08-31 Asuragen, Inc. Methods and compositions involving microRNA
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives
WO2014033326A1 (en) * 2012-09-03 2014-03-06 Qiagen Gmbh Method for isolating rna including small rna with high yield

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