ES2721631T3 - Método para aislar pequeñas moléculas de ARN - Google Patents

Método para aislar pequeñas moléculas de ARN Download PDF

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Publication number
ES2721631T3
ES2721631T3 ES14183292T ES14183292T ES2721631T3 ES 2721631 T3 ES2721631 T3 ES 2721631T3 ES 14183292 T ES14183292 T ES 14183292T ES 14183292 T ES14183292 T ES 14183292T ES 2721631 T3 ES2721631 T3 ES 2721631T3
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ES
Spain
Prior art keywords
lysate
ethanol
solid support
rna molecules
small rna
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Expired - Lifetime
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ES14183292T
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English (en)
Spanish (es)
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Richard C Conrad
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Life Technologies Corp
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Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES14183292T 2003-07-25 2004-07-26 Método para aislar pequeñas moléculas de ARN Expired - Lifetime ES2721631T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49032503P 2003-07-25 2003-07-25
US10/667,126 US20050059024A1 (en) 2003-07-25 2003-09-19 Methods and compositions for isolating small RNA molecules

Publications (1)

Publication Number Publication Date
ES2721631T3 true ES2721631T3 (es) 2019-08-02

Family

ID=34118826

Family Applications (2)

Application Number Title Priority Date Filing Date
ES14183292T Expired - Lifetime ES2721631T3 (es) 2003-07-25 2004-07-26 Método para aislar pequeñas moléculas de ARN
ES18166823T Expired - Lifetime ES2793249T3 (es) 2003-07-25 2004-07-26 Método para aislar moléculas de ARN pequeño

Family Applications After (1)

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ES18166823T Expired - Lifetime ES2793249T3 (es) 2003-07-25 2004-07-26 Método para aislar moléculas de ARN pequeño

Country Status (5)

Country Link
US (5) US20050059024A1 (https=)
EP (4) EP2298891B2 (https=)
JP (1) JP2007500008A (https=)
ES (2) ES2721631T3 (https=)
WO (1) WO2005012523A1 (https=)

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