ECSP034577A - Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado - Google Patents
Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleadoInfo
- Publication number
- ECSP034577A ECSP034577A EC2003004577A ECSP034577A ECSP034577A EC SP034577 A ECSP034577 A EC SP034577A EC 2003004577 A EC2003004577 A EC 2003004577A EC SP034577 A ECSP034577 A EC SP034577A EC SP034577 A ECSP034577 A EC SP034577A
- Authority
- EC
- Ecuador
- Prior art keywords
- typification
- microorganisms
- reagent
- quick
- agent
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 title 1
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 244000045947 parasite Species 0.000 abstract 1
- 238000001853 pulsed-field electrophoresis Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Electrochemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La invención se refiere a que se provee un proceso para tipificar rápidamente levaduras, parásitos y bacterias. Comprende los siguientes pasos: a) Preparar, en 6 a 50 minutos, ADN intacto e inmovilizado mediante un método que emplea un juego de reactivos que solo contiene solución tampón, un detergente, un agente delante y un agente que rompe los puentes de hidrógeno. b) Separar moléculas de ADN intactas o sus fragmentos de restricción usando mini equipos de electroforesis de campos pulsantes de los sistemas CHEF y Tafe.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU20000247A CU23095A1 (es) | 2000-11-07 | 2000-11-07 | Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado |
Publications (1)
Publication Number | Publication Date |
---|---|
ECSP034577A true ECSP034577A (es) | 2003-08-29 |
Family
ID=40134841
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EC2003004577A ECSP034577A (es) | 2000-11-07 | 2003-05-07 | Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado |
Country Status (19)
Country | Link |
---|---|
US (1) | US7731828B2 (es) |
EP (1) | EP1350852B1 (es) |
JP (1) | JP3955817B2 (es) |
CN (1) | CN100532569C (es) |
AT (1) | ATE350490T1 (es) |
AU (1) | AU2002214939A1 (es) |
BR (1) | BR0115172B1 (es) |
CA (1) | CA2428313C (es) |
CU (1) | CU23095A1 (es) |
CY (1) | CY1106369T1 (es) |
DE (1) | DE60125832T2 (es) |
DK (1) | DK1350852T3 (es) |
EC (1) | ECSP034577A (es) |
ES (1) | ES2276843T3 (es) |
HK (1) | HK1063822A1 (es) |
MX (1) | MXPA03004006A (es) |
PT (1) | PT1350852E (es) |
WO (1) | WO2002038802A1 (es) |
ZA (1) | ZA200303472B (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
US20040158447A1 (en) * | 2003-02-07 | 2004-08-12 | Dupont Canada Inc. | Method for simulating and modeling the presence and growth of microbes, including pathogens and spoilage organisms, through a food supply chain |
US20080079726A1 (en) * | 2006-07-03 | 2008-04-03 | Wolfgang Geiger | Visual display of process sequences |
US20080044822A1 (en) * | 2006-08-21 | 2008-02-21 | Gafur Zainiev | Nucleic acid array with releaseable nucleic acid probes |
US20080044821A1 (en) * | 2006-08-21 | 2008-02-21 | Gafur Zainiev | Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes |
US20100056388A1 (en) * | 2006-08-21 | 2010-03-04 | Cnvgenes, Inc. | Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes |
US20090286694A1 (en) * | 2006-08-21 | 2009-11-19 | Gafur Zainiev | Nucleic acid array with releaseable nucleic acid probes |
US9926552B2 (en) * | 2011-06-06 | 2018-03-27 | Cornell University | Microfluidic device for extracting, isolating, and analyzing DNA from cells |
RU2014101490A (ru) | 2011-06-19 | 2015-07-27 | Эйбоджен, Инк. | Устройства, растворы и способы для сбора образцов |
CN103196980B (zh) * | 2013-03-27 | 2014-10-08 | 中国水产科学研究院淡水渔业研究中心 | 鱼源链球菌脉冲场凝胶电泳快速分型方法 |
US9333463B2 (en) | 2013-07-26 | 2016-05-10 | General Electric Company | Devices and systems for elution of biomolecules |
US9999856B2 (en) | 2013-07-26 | 2018-06-19 | General Electric Company | Methods for electroelution of biomolecules |
PL3114225T3 (pl) * | 2014-03-07 | 2021-11-08 | Dna Genotek Inc. | Sposób i kompozycja do stabilizacji kwasów nukleinowych w próbkach biologicznych |
CN105372318B (zh) * | 2014-08-28 | 2018-01-05 | 山东省肿瘤医院 | 将细胞原位电泳与流式分析术结合的细胞固定方法 |
CN104561326B (zh) * | 2015-01-16 | 2017-02-22 | 天津医科大学 | 一种基于菌毛多样性的尿路感染大肠杆菌的分型方法 |
US11383240B2 (en) | 2016-05-22 | 2022-07-12 | Cornell University | Single cell whole genome amplification via micropillar arrays under flow conditions |
WO2018051374A1 (en) * | 2016-09-15 | 2018-03-22 | Dianax S.R.L. | Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample |
KR102388753B1 (ko) * | 2020-07-20 | 2022-04-20 | 광주과학기술원 | 유전영동 힘을 이용한 미생물 검출 장치 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4707237A (en) * | 1982-09-09 | 1987-11-17 | Ciba Corning Diagnostics Corp. | System for identification of cells by electrophoresis |
US4861448A (en) | 1982-11-18 | 1989-08-29 | The Trustees Of Columbia University In The City Of New York | Electrophoretic methods employing gel inserts |
US4473452A (en) * | 1982-11-18 | 1984-09-25 | The Trustees Of Columbia University In The City Of New York | Electrophoresis using alternating transverse electric fields |
CA2052037A1 (en) * | 1990-10-02 | 1992-04-03 | Eric Lai | Automatic control system for improved electrophoresis |
FR2693209B1 (fr) * | 1992-07-03 | 1994-09-30 | Inst Nat Sante Rech Med | Procédé de détermination de la taille en nucléotides de fragments d'ADN. |
CA2176496C (en) | 1993-11-29 | 1999-09-28 | Kathleen A. Clark | Method for extracting nucleic acids from a wide range of organisms |
DE69924900T2 (de) * | 1998-02-16 | 2006-02-23 | Mallinckrodt, Inc. | Behälter für ein elektroforetisches Gel und zugehöriges Verwendungsverfahren |
-
2000
- 2000-11-07 CU CU20000247A patent/CU23095A1/es unknown
-
2001
- 2001-11-02 BR BRPI0115172-0A patent/BR0115172B1/pt not_active IP Right Cessation
- 2001-11-02 AU AU2002214939A patent/AU2002214939A1/en not_active Abandoned
- 2001-11-02 PT PT01983428T patent/PT1350852E/pt unknown
- 2001-11-02 MX MXPA03004006A patent/MXPA03004006A/es active IP Right Grant
- 2001-11-02 DK DK01983428T patent/DK1350852T3/da active
- 2001-11-02 ES ES01983428T patent/ES2276843T3/es not_active Expired - Lifetime
- 2001-11-02 US US10/415,950 patent/US7731828B2/en not_active Expired - Fee Related
- 2001-11-02 AT AT01983428T patent/ATE350490T1/de active
- 2001-11-02 JP JP2002542115A patent/JP3955817B2/ja not_active Expired - Fee Related
- 2001-11-02 EP EP01983428A patent/EP1350852B1/en not_active Expired - Lifetime
- 2001-11-02 CN CNB018203035A patent/CN100532569C/zh not_active Expired - Fee Related
- 2001-11-02 DE DE60125832T patent/DE60125832T2/de not_active Expired - Lifetime
- 2001-11-02 CA CA2428313A patent/CA2428313C/en not_active Expired - Lifetime
- 2001-11-02 WO PCT/CU2001/000008 patent/WO2002038802A1/es active IP Right Grant
-
2003
- 2003-05-06 ZA ZA200303472A patent/ZA200303472B/en unknown
- 2003-05-07 EC EC2003004577A patent/ECSP034577A/es unknown
-
2004
- 2004-09-01 HK HK04106583.6A patent/HK1063822A1/xx not_active IP Right Cessation
-
2007
- 2007-03-12 CY CY20071100340T patent/CY1106369T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
CN1479790A (zh) | 2004-03-03 |
CA2428313A1 (en) | 2002-05-16 |
EP1350852B1 (en) | 2007-01-03 |
BR0115172B1 (pt) | 2014-10-21 |
ZA200303472B (en) | 2003-11-11 |
JP3955817B2 (ja) | 2007-08-08 |
JP2004513375A (ja) | 2004-04-30 |
DE60125832T2 (de) | 2007-10-11 |
PT1350852E (pt) | 2007-03-30 |
CN100532569C (zh) | 2009-08-26 |
MXPA03004006A (es) | 2004-04-02 |
CU23095A1 (es) | 2005-11-18 |
WO2002038802A1 (es) | 2002-05-16 |
BR0115172A (pt) | 2004-02-17 |
US20040050700A1 (en) | 2004-03-18 |
DK1350852T3 (da) | 2007-05-07 |
AU2002214939A1 (en) | 2002-05-21 |
DE60125832D1 (de) | 2007-02-15 |
CA2428313C (en) | 2010-09-14 |
EP1350852A1 (en) | 2003-10-08 |
ATE350490T1 (de) | 2007-01-15 |
US7731828B2 (en) | 2010-06-08 |
HK1063822A1 (en) | 2005-01-14 |
ES2276843T3 (es) | 2007-07-01 |
CY1106369T1 (el) | 2011-10-12 |
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