ECSP034577A - Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado - Google Patents

Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado

Info

Publication number
ECSP034577A
ECSP034577A EC2003004577A ECSP034577A ECSP034577A EC SP034577 A ECSP034577 A EC SP034577A EC 2003004577 A EC2003004577 A EC 2003004577A EC SP034577 A ECSP034577 A EC SP034577A EC SP034577 A ECSP034577 A EC SP034577A
Authority
EC
Ecuador
Prior art keywords
typification
microorganisms
reagent
quick
agent
Prior art date
Application number
EC2003004577A
Other languages
English (en)
Inventor
Canovas Lilia Lopez
Clarke David Higgison
Rojas Ana Maria Riveron
Alonso Axel Sanchez
Orozco Esther Orozco
Diaz Oscar Arencibia
Acuna Maria Ariosa
Donderiz Hilda Teresa Clark
Perez Regnar Gigato
Original Assignee
Centro Nacional De Investigaciones Cientificas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Nacional De Investigaciones Cientificas filed Critical Centro Nacional De Investigaciones Cientificas
Publication of ECSP034577A publication Critical patent/ECSP034577A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La invención se refiere a que se provee un proceso para tipificar rápidamente levaduras, parásitos y bacterias. Comprende los siguientes pasos: a) Preparar, en 6 a 50 minutos, ADN intacto e inmovilizado mediante un método que emplea un juego de reactivos que solo contiene solución tampón, un detergente, un agente delante y un agente que rompe los puentes de hidrógeno. b) Separar moléculas de ADN intactas o sus fragmentos de restricción usando mini equipos de electroforesis de campos pulsantes de los sistemas CHEF y Tafe.
EC2003004577A 2000-11-07 2003-05-07 Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado ECSP034577A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Publications (1)

Publication Number Publication Date
ECSP034577A true ECSP034577A (es) 2003-08-29

Family

ID=40134841

Family Applications (1)

Application Number Title Priority Date Filing Date
EC2003004577A ECSP034577A (es) 2000-11-07 2003-05-07 Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado

Country Status (19)

Country Link
US (1) US7731828B2 (es)
EP (1) EP1350852B1 (es)
JP (1) JP3955817B2 (es)
CN (1) CN100532569C (es)
AT (1) ATE350490T1 (es)
AU (1) AU2002214939A1 (es)
BR (1) BR0115172B1 (es)
CA (1) CA2428313C (es)
CU (1) CU23095A1 (es)
CY (1) CY1106369T1 (es)
DE (1) DE60125832T2 (es)
DK (1) DK1350852T3 (es)
EC (1) ECSP034577A (es)
ES (1) ES2276843T3 (es)
HK (1) HK1063822A1 (es)
MX (1) MXPA03004006A (es)
PT (1) PT1350852E (es)
WO (1) WO2002038802A1 (es)
ZA (1) ZA200303472B (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040158447A1 (en) * 2003-02-07 2004-08-12 Dupont Canada Inc. Method for simulating and modeling the presence and growth of microbes, including pathogens and spoilage organisms, through a food supply chain
US20080079726A1 (en) * 2006-07-03 2008-04-03 Wolfgang Geiger Visual display of process sequences
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US20090286694A1 (en) * 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20080044822A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20100056388A1 (en) * 2006-08-21 2010-03-04 Cnvgenes, Inc. Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
WO2012170560A2 (en) * 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
EP2721140B1 (en) 2011-06-19 2016-11-23 Abogen, Inc. Devices, solutions and methods for sample collection
CN103196980B (zh) * 2013-03-27 2014-10-08 中国水产科学研究院淡水渔业研究中心 鱼源链球菌脉冲场凝胶电泳快速分型方法
US9999856B2 (en) 2013-07-26 2018-06-19 General Electric Company Methods for electroelution of biomolecules
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
PT3114225T (pt) * 2014-03-07 2021-07-26 Dna Genotek Inc Composição e método para estabilização de ácidos nucleicos em amostras biológicas
CN105372318B (zh) * 2014-08-28 2018-01-05 山东省肿瘤医院 将细胞原位电泳与流式分析术结合的细胞固定方法
CN104561326B (zh) * 2015-01-16 2017-02-22 天津医科大学 一种基于菌毛多样性的尿路感染大肠杆菌的分型方法
US11383240B2 (en) 2016-05-22 2022-07-12 Cornell University Single cell whole genome amplification via micropillar arrays under flow conditions
WO2018051374A1 (en) 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
KR102388753B1 (ko) * 2020-07-20 2022-04-20 광주과학기술원 유전영동 힘을 이용한 미생물 검출 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707237A (en) * 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US4861448A (en) * 1982-11-18 1989-08-29 The Trustees Of Columbia University In The City Of New York Electrophoretic methods employing gel inserts
US4473452A (en) 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
CA2052037A1 (en) * 1990-10-02 1992-04-03 Eric Lai Automatic control system for improved electrophoresis
FR2693209B1 (fr) * 1992-07-03 1994-09-30 Inst Nat Sante Rech Med Procédé de détermination de la taille en nucléotides de fragments d'ADN.
WO1995014768A2 (en) * 1993-11-29 1995-06-01 Gen-Probe Incorporated Method for extracting nucleic acids from a wide range of organisms
ATE294386T1 (de) * 1998-02-16 2005-05-15 Mallinckrodt Inc Behälter für ein elektroforetisches gel und zugehöriges verwendungsverfahren

Also Published As

Publication number Publication date
CY1106369T1 (el) 2011-10-12
ES2276843T3 (es) 2007-07-01
PT1350852E (pt) 2007-03-30
CA2428313C (en) 2010-09-14
DK1350852T3 (da) 2007-05-07
HK1063822A1 (en) 2005-01-14
US7731828B2 (en) 2010-06-08
ZA200303472B (en) 2003-11-11
ATE350490T1 (de) 2007-01-15
JP3955817B2 (ja) 2007-08-08
WO2002038802A1 (es) 2002-05-16
CU23095A1 (es) 2005-11-18
BR0115172B1 (pt) 2014-10-21
DE60125832D1 (de) 2007-02-15
CN1479790A (zh) 2004-03-03
EP1350852B1 (en) 2007-01-03
JP2004513375A (ja) 2004-04-30
US20040050700A1 (en) 2004-03-18
CA2428313A1 (en) 2002-05-16
AU2002214939A1 (en) 2002-05-21
BR0115172A (pt) 2004-02-17
EP1350852A1 (en) 2003-10-08
CN100532569C (zh) 2009-08-26
MXPA03004006A (es) 2004-04-02
DE60125832T2 (de) 2007-10-11

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