BR0115172A - Processo para a tipificação rápida de microorganismos, e, conjunto de reativos - Google Patents

Processo para a tipificação rápida de microorganismos, e, conjunto de reativos

Info

Publication number
BR0115172A
BR0115172A BR0115172-0A BR0115172A BR0115172A BR 0115172 A BR0115172 A BR 0115172A BR 0115172 A BR0115172 A BR 0115172A BR 0115172 A BR0115172 A BR 0115172A
Authority
BR
Brazil
Prior art keywords
microorganisms
employing
intact
field electrophoresis
minichef
Prior art date
Application number
BR0115172-0A
Other languages
English (en)
Other versions
BR0115172B1 (pt
Inventor
Lilia Lopez Canovas
Ana Maria Riveron Rojas
David Higgison Clarke
Axel Sanchez Alonso
Esther Orozco Orozco
Oscar Arencibia Diaz
Concepcion Ariosa Acu A Maria
Hilda Teresa Clark Donderiz
Regnar Gigato Perez
Original Assignee
Cnic Ct Nac Investigaciones
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cnic Ct Nac Investigaciones filed Critical Cnic Ct Nac Investigaciones
Publication of BR0115172A publication Critical patent/BR0115172A/pt
Publication of BR0115172B1 publication Critical patent/BR0115172B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

"PROCESSO PARA A TIPIFICAçãO RáPIDA DE MICROORGANISMOS, E, CONJUNTO DE REATIVOS". Proporciona-se um processo para tipificar rapidamente leveduras, parasitas e bactérias. Compreende as seguintes etapas: a) Preparar, em de 5 a 60 minutos, DNA intacto e imobilizado mediante um método que emprega um conjunto de reativos que apenas contém solução tampão, um detergente, um agente quelante e um agente que rompe as pontes de hidrogênio. b) Separar moléculas de DNA intactas ou seus fragmentos de restrição utilizando-se n-úni-equipamentos de eletroforese de campos pulsantes dos sistemas CHEF (Contour Clamped Homogeneous Electric Field) e TAFE (Transversal Altemating Field Electrophoresis) em tempos compreendidos entre 2,5 e 7 horas. c) Selecionar as condições ótimas que serão aplicadas no miniCHEF empregando-se um método que simula a priori os padrões eletroforéticos que se obteria nos referidos géis. d) Proporcionar os tempos de reorientação, velocidades de migração e tamanhos das moléculas sem empregar marcadores de tamanhos, porém valendo-se de um método que analisa distâncias migradas.
BRPI0115172-0A 2000-11-07 2001-11-02 Processo para a tipificação bacteriana rápida por meio de eletroforese de campo pulsado em gel BR0115172B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CU2000/0247 2000-11-07
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
PCT/CU2001/000008 WO2002038802A1 (es) 2000-11-07 2001-11-02 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Publications (2)

Publication Number Publication Date
BR0115172A true BR0115172A (pt) 2004-02-17
BR0115172B1 BR0115172B1 (pt) 2014-10-21

Family

ID=40134841

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0115172-0A BR0115172B1 (pt) 2000-11-07 2001-11-02 Processo para a tipificação bacteriana rápida por meio de eletroforese de campo pulsado em gel

Country Status (19)

Country Link
US (1) US7731828B2 (pt)
EP (1) EP1350852B1 (pt)
JP (1) JP3955817B2 (pt)
CN (1) CN100532569C (pt)
AT (1) ATE350490T1 (pt)
AU (1) AU2002214939A1 (pt)
BR (1) BR0115172B1 (pt)
CA (1) CA2428313C (pt)
CU (1) CU23095A1 (pt)
CY (1) CY1106369T1 (pt)
DE (1) DE60125832T2 (pt)
DK (1) DK1350852T3 (pt)
EC (1) ECSP034577A (pt)
ES (1) ES2276843T3 (pt)
HK (1) HK1063822A1 (pt)
MX (1) MXPA03004006A (pt)
PT (1) PT1350852E (pt)
WO (1) WO2002038802A1 (pt)
ZA (1) ZA200303472B (pt)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040158447A1 (en) * 2003-02-07 2004-08-12 Dupont Canada Inc. Method for simulating and modeling the presence and growth of microbes, including pathogens and spoilage organisms, through a food supply chain
US20080079726A1 (en) * 2006-07-03 2008-04-03 Wolfgang Geiger Visual display of process sequences
US20090286694A1 (en) * 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US20080044822A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20100056388A1 (en) * 2006-08-21 2010-03-04 Cnvgenes, Inc. Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
WO2012170560A2 (en) * 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
CN106442039B (zh) 2011-06-19 2020-08-07 阿博根公司 用于样品采集的装置、溶液和方法
CN103196980B (zh) * 2013-03-27 2014-10-08 中国水产科学研究院淡水渔业研究中心 鱼源链球菌脉冲场凝胶电泳快速分型方法
US9999856B2 (en) 2013-07-26 2018-06-19 General Electric Company Methods for electroelution of biomolecules
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
JP6670765B2 (ja) * 2014-03-07 2020-03-25 ディーエヌエー ジェノテック インク 生物試料中の核酸を安定化する組成物および方法
CN105372318B (zh) * 2014-08-28 2018-01-05 山东省肿瘤医院 将细胞原位电泳与流式分析术结合的细胞固定方法
CN104561326B (zh) * 2015-01-16 2017-02-22 天津医科大学 一种基于菌毛多样性的尿路感染大肠杆菌的分型方法
WO2017205304A1 (en) 2016-05-22 2017-11-30 Cornell University Single cell whole genome amplification via micropillar arrays under flow conditions
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
KR102388753B1 (ko) * 2020-07-20 2022-04-20 광주과학기술원 유전영동 힘을 이용한 미생물 검출 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707237A (en) * 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US4473452A (en) * 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
US4861448A (en) * 1982-11-18 1989-08-29 The Trustees Of Columbia University In The City Of New York Electrophoretic methods employing gel inserts
CA2052037A1 (en) * 1990-10-02 1992-04-03 Eric Lai Automatic control system for improved electrophoresis
FR2693209B1 (fr) * 1992-07-03 1994-09-30 Inst Nat Sante Rech Med Procédé de détermination de la taille en nucléotides de fragments d'ADN.
CA2176496C (en) 1993-11-29 1999-09-28 Kathleen A. Clark Method for extracting nucleic acids from a wide range of organisms
EP0936464B1 (en) * 1998-02-16 2005-04-27 Mallinckrodt Inc. Electrophoresis gel container apparatus and method of use thereof

Also Published As

Publication number Publication date
ES2276843T3 (es) 2007-07-01
MXPA03004006A (es) 2004-04-02
EP1350852A1 (en) 2003-10-08
DK1350852T3 (da) 2007-05-07
ZA200303472B (en) 2003-11-11
US7731828B2 (en) 2010-06-08
HK1063822A1 (en) 2005-01-14
CN1479790A (zh) 2004-03-03
DE60125832D1 (de) 2007-02-15
WO2002038802A1 (es) 2002-05-16
ATE350490T1 (de) 2007-01-15
US20040050700A1 (en) 2004-03-18
CY1106369T1 (el) 2011-10-12
CN100532569C (zh) 2009-08-26
CA2428313A1 (en) 2002-05-16
PT1350852E (pt) 2007-03-30
CA2428313C (en) 2010-09-14
DE60125832T2 (de) 2007-10-11
EP1350852B1 (en) 2007-01-03
JP2004513375A (ja) 2004-04-30
CU23095A1 (es) 2005-11-18
ECSP034577A (es) 2003-08-29
AU2002214939A1 (en) 2002-05-21
BR0115172B1 (pt) 2014-10-21
JP3955817B2 (ja) 2007-08-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]

Free format text: NOTIFICACAO DE DEVOLUCAO DO PEDIDO POR NAO SE ENQUADRAR NO ART. 229-C DA LPI.

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 21/10/2014, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 15A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2385 DE 20-09-2016 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.