JP2014507155A5 - - Google Patents
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- Publication number
- JP2014507155A5 JP2014507155A5 JP2013554672A JP2013554672A JP2014507155A5 JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5 JP 2013554672 A JP2013554672 A JP 2013554672A JP 2013554672 A JP2013554672 A JP 2013554672A JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- solution
- beads
- washing
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 239000011324 bead Substances 0.000 claims 20
- 238000000034 method Methods 0.000 claims 16
- 238000000746 purification Methods 0.000 claims 16
- 238000005406 washing Methods 0.000 claims 14
- 239000011159 matrix material Substances 0.000 claims 8
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 4
- 239000003960 organic solvent Substances 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 238000011156 evaluation Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 239000012488 sample solution Substances 0.000 claims 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161444952P | 2011-02-21 | 2011-02-21 | |
| US61/444,952 | 2011-02-21 | ||
| PCT/US2012/025861 WO2012115926A2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014507155A JP2014507155A (ja) | 2014-03-27 |
| JP2014507155A5 true JP2014507155A5 (https=) | 2015-04-09 |
| JP6113083B2 JP6113083B2 (ja) | 2017-04-19 |
Family
ID=46653044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013554672A Expired - Fee Related JP6113083B2 (ja) | 2011-02-21 | 2012-02-21 | マイクロ流体素子を基礎とした核酸精製方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8722329B2 (https=) |
| EP (1) | EP2678429B1 (https=) |
| JP (1) | JP6113083B2 (https=) |
| AU (1) | AU2012220825B2 (https=) |
| WO (1) | WO2012115926A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2678429B1 (en) * | 2011-02-21 | 2018-04-18 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| US11427815B2 (en) | 2015-12-28 | 2022-08-30 | Koninklijke Philips N.V. | Nucleic acid purification system using a single wash and elution buffer solution |
| DE102017206155A1 (de) | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| EP3611508B1 (en) * | 2017-05-16 | 2022-08-03 | SK Telecom Co., Ltd. | Nucleic acid analysis apparatus using catridge |
| GB2591406B (en) * | 2018-09-05 | 2023-03-01 | Ihsan Dogramaci Bilkent Univ | Enrichment of samples inside the microchannels by using magnetic particles |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN115820412B (zh) * | 2022-11-17 | 2025-12-02 | 北京昌平实验室 | 一种从复杂样本中特异检测核酸的微流控卡盒及方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3788082T2 (de) * | 1986-04-11 | 1994-06-09 | Applied Biosystems | Automatisches Gerät zum Extrahieren von Nukleinsäuren. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US6037465A (en) | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| WO1999040098A1 (de) | 1998-02-04 | 1999-08-12 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| WO2003057910A2 (en) | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Use of silica material in an amplification reaction |
| EP1388588B1 (en) * | 2002-07-29 | 2008-09-17 | JSR Corporation | Nucleic acid-separating method and nucleic acid-extracting reagent |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| EP2261650A3 (en) * | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| JP4980349B2 (ja) | 2005-06-23 | 2012-07-18 | シーメンス メディカル ソリューションズ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 閉鎖された超薄シリカ層を有する磁性粒子、その製造方法および使用 |
| US7976795B2 (en) * | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| WO2008116182A1 (en) | 2007-03-21 | 2008-09-25 | Ibis Biosciences, Inc. | Reagents for nucleic acid purification |
| JP5523327B2 (ja) * | 2007-10-12 | 2014-06-18 | レオニックス，インコーポレイテッド | 統合型マイクロ流体デバイスおよび方法 |
| KR20110030415A (ko) * | 2008-01-22 | 2011-03-23 | 인터젠엑스 인크. | 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도 |
| EP2394152B1 (en) * | 2009-02-03 | 2019-05-01 | ANDE Corporation | Nucleic acid purification |
| US20120164036A1 (en) * | 2009-07-21 | 2012-06-28 | Seth Stern | Microfluidic devices and uses thereof |
| EP2678429B1 (en) * | 2011-02-21 | 2018-04-18 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
-
2012
- 2012-02-21 EP EP12749025.8A patent/EP2678429B1/en active Active
- 2012-02-21 AU AU2012220825A patent/AU2012220825B2/en not_active Ceased
- 2012-02-21 US US13/400,857 patent/US8722329B2/en active Active
- 2012-02-21 JP JP2013554672A patent/JP6113083B2/ja not_active Expired - Fee Related
- 2012-02-21 WO PCT/US2012/025861 patent/WO2012115926A2/en not_active Ceased
-
2014
- 2014-03-26 US US14/226,204 patent/US9169479B2/en active Active
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