WO2020050809A2 - Enrichment of samples inside the microchannels by using magnetic particles - Google Patents
Enrichment of samples inside the microchannels by using magnetic particles Download PDFInfo
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- WO2020050809A2 WO2020050809A2 PCT/TR2019/050728 TR2019050728W WO2020050809A2 WO 2020050809 A2 WO2020050809 A2 WO 2020050809A2 TR 2019050728 W TR2019050728 W TR 2019050728W WO 2020050809 A2 WO2020050809 A2 WO 2020050809A2
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- biological
- purification
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- enrichment
- magnetic particles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Definitions
- This invention relates to purification method for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, wherein it is particularly microfluidic based biological and/or chemical sample purification method by customized magnetic particles.
- Purification/enrichment methods by using magnetic particles are generally used to isolate a target material such as nucleic acid and protein from a biological sample.
- biochemical substances in suspended form are rapidly bound to magnetic particles having a large surface area and magnetic area is applied to collect the magnetic particles to which the target material is bound.
- various equipment and methods have been developed.
- This technique comprises the use of paramagnetic particles and US application with publication number US5973138 discloses the details of said technique.
- the paramagnetic particles are added in an acidic solution together with biological samples in said developed method. Particularly when cell samples are dissolved to release nucleic acids, the nucleic acids are bound to the paramagnetic particles in a manner to be reversible. Then the magnetic particles can be separated from the remaining of the solution by known techniques such as centrifuge, filtering or magnetic force.
- the magnetic particle to which the nucleic acids are bound can be removed from the solution and can be added in a suitable buffer solution causing the nucleic acids to be separated from the magnetic particles. Then the magnetic particles can be separated from nucleic acids by any of the prior art methods.
- the magnetic purification is performed generally in two ways when it is for biological samples. The first one them is that the samples to be purified exhibit magnetic moment per se and that the magnetic purification is carried out without any modification. However, these samples having magnetic characteristic are only blood cells and magnetotactic cells. In the purification process intended for all biological samples other than those, magnetically labeled tail is attached to one or more non-magnetic components in a solution.
- purification/enrichment methods by using magnetic particles can be used for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, they can also be used in purification/enrichment of chemical and mineral ores.
- Enrichment in mining industry are the studies conducted for purifying mineral substrates (ores) to obtain valuable minerals.
- Studies carried out in this field include the separation of minerals desired or defined as valuable from the other substrates that may be contained in the mineral substrate.
- Some mineral decomposition processes include the use of magnetic reactive and strong magnetic fields.
- US Patent Application with publication No US4643822 discloses a method for separation of constituent minerals of a mixture of minerals comprising mixed fine particles of magnetic materials such as finely ground magnetic particles having the mixture of minerals in the presence of a surfactant.
- One of the problems encountered is the fact that the systems used in the studies carried out by means of the interaction with magnetic particles have macro-scale and/or complex structures. Furthermore, in said macro systems, solutions of large amount are needed for the systems designed for purification/enrichment of samples. Particularly, in purification process of some biological samples, the purification process cannot be performed since the amount of samples cannot be high.
- the suspension which is desired to be separated in the agglomeration is passed through a channel, the magnetic particles are generally packed inside the channels by the effect of the magnetic force acting on it.
- the magnetic particles are separated from nonmagnetic contaminants by being packed in said channels.
- Structures such as glass beads are used for packaging process inside the channel.
- the interaction of the magnetic particles and the solutions used herein cannot at the desired level because of the structures such as glass beads located inside the channel and ensuring the packaging.
- the interaction surface areas of magnetic particles expected to pass around these spherical structures are inadequate because of the structures of spherical glass beads and their closely arrangement inside the channel.
- US Patent Applications Publication No. US5702950 and US 6231814 relate to controlling of magnetic field towards intended direction of capillary channel by connection and removing a magnet, which is for attraction and releasing of magnetic substance and for controlling them, to end of a capillary channel.
- a channel device having a liquid suction line at a liquid inlet end and magnet bodies attached detachably at the outer surface of the liquid suction line of the channel device for receiving the liquid containing the magnetic material from a container and discharging the liquid through the liquid inlet end.
- German Patent Application with the publication No. DE19500660A1 discloses a manipulator for microscopic particles using a novel ultra-microelectrode system that generates high frequency fields and a strong local field.
- microcentrifuge tube has been used for purification and the yield and quality of DNA depend greatly on the quality of the starting material, the number of cells per sample and the genome size of the sample source.
- the object of the invention is that purification/enrichment process, which enables use of sample at microscale and low amounts thereby enabling control of microparticles as desired manner, is rapidly performed in order to eliminate the drawbacks encountered in magnetic purification/enrichment methods which are micro scaled having complex structures and are macro scaled requiring high content of sample usage.
- Another object of the invention is to allow for working particularly with biological samples exhibiting rapid degradation by performing purification/enrichment process in a quick manner.
- Another object of the invention is to increase the interaction surface of the particles with the buffer solution and to enable the particles to retain biological and/or chemical samples more effectively by means of a system working with developed microfluidic based customized magnetic particles.
- Another object of the invention is that the particles capture biological and/or chemical samples in the solution by different interaction mechanisms and provide the necessary purification/enrichment.
- the present invention provides faster purification technique and it can be carried out with fewer sample amounts by comparison with the purification techniques used in prior art.
- the invention relates to a method for manipulating magnetic particles in a liquid sample for efficiently collecting biological and/or chemical samples which were bound to the particles inside a solution.
- the invention particularly relates to a method for the purification/enrichment of biological and/or chemical samples by microfluidic based, customized magnetic particles.
- Magnetic particles are grains that can be directed under magnetic field.
- the magnetic particles located within the microchannels are manipulated by any controllable magnetic field.
- a solution of the samples intended to be purified/enriched and to be passed through the microchannel is bound to magnetic particles customized according to said samples (which may interact with the sample to be purified by coating, bonding, etc.) and located inside the microchannel during its passage through the microchannel.
- the solution of the samples passed through the microchannel interacts with the magnetic particles located inside the microchannel and customized according to the sample within this channel.
- Said interaction occurs when the magnetic particles inside the channel under the influence of the magnetic field are in the same direction as the flow direction of the solution of sample, or in the reverse direction of the flow direction of the solution of sample or suspended in the solution of sample (free oscillation in the solution of sample).
- the microchannels through which the sample solution passes, and in which the magnetic particles are located can be circular/spiral, flat or in different geometric shapes according to their area of usage.
- Said microchannels may optionally be formed on a magnetic stand by micro/nano patterning.
- the sample solution entering through the channel encounters here the magnetic particles.
- the magnetic particles exhibit a behavior towards the direction of flow of the sample solution inside the channel and in terms of the forces exerted on it by the effect of the magnetic field. While the magnetic particles flow through the microchannel together with the sample solution passing through the microchannel on the one hand, they can also be guided by the adjustable magnetic field with the contribution of factors such as magnetic sensitivity, size, channel form, flow rate, etc. on the other hand. Retention/purification process of biological and/or chemical samples inside the sample solution is implemented such that the volume of sample solution entering through the microchannel will be maximum 10pl.
- the magnetic particles to be positioned inside the subject matter microchannels may be comprised of paramagnetic or diamagnetic particles or mixture thereof.
- paramagnetic particles having positive substance magnetization degree, magnetic dipole moment at unit volume and the magnetic field intensity have the same direction, namely they have orientation to magnetic field effect from which they are influenced, and which are applied to them.
- the diamagnetic particles has negative substance magnetization degree, and its magnetic dipole moment at unit volume and its magnetic field intensity have the opposite direction.
- the samples which are bound to the magnetic particles are subjected to a magnetic field to separate them from the solution and from unbound contaminants.
- the direction of the magnetic field is determined by turning the platform, on which the magnets are fixed, by means of a motor.
- clockwise or counter-clockwise rotations of the particles can be controlled with the selected rotation of the motor.
- the particles can be moved in the same or reverse direction as the flow direction according to the flow rate and magnitude of magnetic field applied or can be suspended inside the fluid (when drag force due to flow is in different direction with the magnetic force and equal in size with it) by selecting the flow direction according to movement direction of particles inside the magnetic field.
- the magnetic field used in the invention and being controllable can be generated by means of current as well as by permanent magnets such as electromagnetic field/electromagnet.
- the operation of the invention has been demonstrated by preliminary proof studies with fish sperm DNA using the new platform related to the invention.
- magnetic microparticles were charged in the microchannel and the microfluidic chip was placed on the magnetic platform.
- the adsorption liquid with pH 6.5 was passed through the channel for 2 minutes and the surface of the magnetic microparticles was brought to suitable conditions for DNA adsorption.
- 10pl of approximately 200-300 base pair fish sperm DNA was passed through the microchannel and sample collected at the outlet.
- the amount of DNA retained by the magnetic particles in the microchannel was calculated from this collected sample.
- DNA retained on the magnetic particles by using the new magnetic platform was recovered by desorption liquid with pH 8.5 and analyzed. All treatments lasted an average of 20 minutes in total, and 10-15 pg of DNA was retrieved from the system with new platform per 1 mg particle.
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Abstract
This invention relates to purification method for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, wherein it is particularly microfluidic based biological and/or chemical sample purification method by customized magnetic particles.
Description
ENRICHMENT OF SAMPLES INSIDE THE MICROCHANNELS BY USING
MAGNETIC PARTICLES
Related Technical Field
This invention relates to purification method for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, wherein it is particularly microfluidic based biological and/or chemical sample purification method by customized magnetic particles.
State of the art
Purification/enrichment methods by using magnetic particles are generally used to isolate a target material such as nucleic acid and protein from a biological sample. In those methods, biochemical substances in suspended form are rapidly bound to magnetic particles having a large surface area and magnetic area is applied to collect the magnetic particles to which the target material is bound. For these methods in question, various equipment and methods have been developed.
Recently, a technique which allows nucleic acids to be separated from the remaining constituents of cells more efficiently has been developed. This technique comprises the use of paramagnetic particles and US application with publication number US5973138 discloses the details of said technique. The paramagnetic particles are added in an acidic solution together with biological samples in said developed method. Particularly when cell samples are dissolved to release nucleic acids, the nucleic acids are bound to the paramagnetic particles in a manner to be reversible. Then the magnetic particles can be separated from the remaining of the solution by known techniques such as centrifuge, filtering or magnetic force. Subsequently, the magnetic particle to which the nucleic acids are bound can be removed from the solution and can be added in a suitable buffer solution causing the nucleic acids to be separated from the magnetic particles. Then the magnetic particles can be separated from nucleic acids by any of the prior art methods.
The magnetic purification is performed generally in two ways when it is for biological samples. The first one them is that the samples to be purified exhibit magnetic moment per se and that the magnetic purification is carried out without any modification. However, these samples having magnetic characteristic are only blood cells and magnetotactic cells. In the purification process intended for all biological samples other than those, magnetically labeled tail is attached to one or more non-magnetic components in a solution. By means of these solutions customized according to type of the sample desired to be purified, magnetic sensitivity to be between the sample and the environment is ensured. This method is generally used to purify biological samples in the state of the art, and inside of a channel having one two inlets and one outlet, magnetic particles are passed through one side of inlet section, and tail attached samples and particles having no magnetic sensitivity which are desired to be purified are passed through the other inlet section. Tail attached samples and magnetic particles which come across at the joint of channel encounter and interact here, and while the magnetic particles and tail attached samples held thereon are attracted by a magnet retained outside the channel, those not having magnetic sensitivity continue moving at flow direction on the side of the channel distant from the magnet since the flow from the contaminants are laminar. It is ensured at the end of the flow that the particles and contaminants approaching to the channel wall where the magnet is positioned are removed separately. Thus, it is ensured that the sample to be purified and the undesired particles are separated from each other.
As purification/enrichment methods by using magnetic particles can be used for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, they can also be used in purification/enrichment of chemical and mineral ores.
Enrichment in mining industry are the studies conducted for purifying mineral substrates (ores) to obtain valuable minerals. Studies carried out in this field include the separation of minerals desired or defined as valuable from the other substrates that may be contained in the mineral substrate.
Some mineral decomposition processes include the use of magnetic reactive and strong magnetic fields. US Patent Application with publication No US4643822 discloses a method for separation of constituent minerals of a mixture of minerals comprising mixed fine particles of magnetic materials such as finely ground magnetic particles having the mixture of minerals in the presence of a surfactant. One of the problems encountered is the fact that the systems used in the studies carried out by means of the interaction with magnetic particles have macro-scale and/or complex structures. Furthermore, in said macro systems, solutions of large amount are needed for the systems designed for purification/enrichment of samples. Particularly, in purification process of some biological samples, the purification process cannot be performed since the amount of samples cannot be high.
While magnetic separation has different area of usage in the references, as result of this it is classified in different manners. It is generally examined in two ways as agglomeration and continuous separation depending on the flow rather than the particle used. When the suspension which is desired to be separated in the agglomeration is passed through a channel, the magnetic particles are generally packed inside the channels by the effect of the magnetic force acting on it. The magnetic particles are separated from nonmagnetic contaminants by being packed in said channels. Structures such as glass beads are used for packaging process inside the channel. The interaction of the magnetic particles and the solutions used herein cannot at the desired level because of the structures such as glass beads located inside the channel and ensuring the packaging. In technical solutions wherein structures such as glass beads are used as disclosed in Japanese Patent Application Publication No. JP2004333401 , the interaction surface areas of magnetic particles expected to pass around these spherical structures are inadequate because of the structures of spherical glass beads and their closely arrangement inside the channel.
Concerning the purification of biological samples, US Patent Applications Publication No. US5702950 and US 6231814 relate to controlling of magnetic field towards intended direction of capillary channel by connection and removing a magnet, which is for attraction and releasing of magnetic substance and for controlling them, to end of a capillary channel. In the controlling method of attracting and releasing of this magnetic material, there is a channel device having a liquid suction line at a liquid inlet end and magnet bodies attached detachably at the outer surface of the liquid suction
line of the channel device for receiving the liquid containing the magnetic material from a container and discharging the liquid through the liquid inlet end.
German Patent Application with the publication No. DE19500660A1 discloses a manipulator for microscopic particles using a novel ultra-microelectrode system that generates high frequency fields and a strong local field.
In the research of Konova I. et al., lipogenesis in 2 initial and 4 mutant strains of Penicillium chrysogenum has been investigated. In this research, Eppendorf type centrifugation was used for purification and the yield depends on quantity and source of sample. For example, it yields 4-6 pg DNA from 200 pi of blood, 10-15 pg from mouse heart and 15-20 pg from 2x106 HeLa cells according to this research.
In the research studied by Shibata Y. et al, solution and buffers technique has been used for purification and the genomic DNA can be isolated from 50 mg of tissue or 1 x 107 to 3 x 107 cells with one milliliter of reagent used in that technique.
In the research of Agrawal N. et al, microcentrifuge tube has been used for purification and the yield and quality of DNA depend greatly on the quality of the starting material, the number of cells per sample and the genome size of the sample source.
Advantages Provided by the Invention
The object of the invention is that purification/enrichment process, which enables use of sample at microscale and low amounts thereby enabling control of microparticles as desired manner, is rapidly performed in order to eliminate the drawbacks encountered in magnetic purification/enrichment methods which are micro scaled having complex structures and are macro scaled requiring high content of sample usage.
Another object of the invention is to allow for working particularly with biological samples exhibiting rapid degradation by performing purification/enrichment process in a quick manner.
Another object of the invention is to increase the interaction surface of the particles with the buffer solution and to enable the particles to retain biological and/or chemical samples more effectively by means of a system working with developed microfluidic based customized magnetic particles.
Another object of the invention is that the particles capture biological and/or chemical samples in the solution by different interaction mechanisms and provide the necessary purification/enrichment.
The present invention provides faster purification technique and it can be carried out with fewer sample amounts by comparison with the purification techniques used in prior art.
Description of the Invention
The invention relates to a method for manipulating magnetic particles in a liquid sample for efficiently collecting biological and/or chemical samples which were bound to the particles inside a solution.
The invention particularly relates to a method for the purification/enrichment of biological and/or chemical samples by microfluidic based, customized magnetic particles.
Magnetic particles are grains that can be directed under magnetic field. In the subject matter method set forth, the magnetic particles located within the microchannels are manipulated by any controllable magnetic field. A solution of the samples intended to be purified/enriched and to be passed through the microchannel is bound to magnetic particles customized according to said samples (which may interact with the sample to be purified by coating, bonding, etc.) and located inside the microchannel during its passage through the microchannel. The solution of the samples passed through the microchannel interacts with the magnetic particles located inside the microchannel and customized according to the sample within this channel. Said interaction occurs when the magnetic particles inside the channel under the influence of the magnetic field are in the same direction as the flow direction of the solution of sample, or in the reverse direction of the flow direction of the solution of sample or suspended in the solution of sample (free oscillation in the solution of sample).
In the retention/purification process of the subject matter biological and/or chemical samples, the microchannels through which the sample solution passes, and in which the magnetic particles are located can be circular/spiral, flat or in different geometric
shapes according to their area of usage. Said microchannels may optionally be formed on a magnetic stand by micro/nano patterning.
The sample solution entering through the channel encounters here the magnetic particles. The magnetic particles exhibit a behavior towards the direction of flow of the sample solution inside the channel and in terms of the forces exerted on it by the effect of the magnetic field. While the magnetic particles flow through the microchannel together with the sample solution passing through the microchannel on the one hand, they can also be guided by the adjustable magnetic field with the contribution of factors such as magnetic sensitivity, size, channel form, flow rate, etc. on the other hand. Retention/purification process of biological and/or chemical samples inside the sample solution is implemented such that the volume of sample solution entering through the microchannel will be maximum 10pl.
The magnetic particles to be positioned inside the subject matter microchannels may be comprised of paramagnetic or diamagnetic particles or mixture thereof.
In paramagnetic particles, having positive substance magnetization degree, magnetic dipole moment at unit volume and the magnetic field intensity have the same direction, namely they have orientation to magnetic field effect from which they are influenced, and which are applied to them. The diamagnetic particles has negative substance magnetization degree, and its magnetic dipole moment at unit volume and its magnetic field intensity have the opposite direction.
The samples which are bound to the magnetic particles are subjected to a magnetic field to separate them from the solution and from unbound contaminants. The direction of the magnetic field is determined by turning the platform, on which the magnets are fixed, by means of a motor. Depending on whether the particles are paramagnetic or diamagnetic, clockwise or counter-clockwise rotations of the particles can be controlled with the selected rotation of the motor. The particles can be moved in the same or reverse direction as the flow direction according to the flow rate and magnitude of magnetic field applied or can be suspended inside the fluid (when drag force due to flow is in different direction with the magnetic force and equal in size with it) by selecting the flow direction according to movement direction of particles inside the magnetic field.
The magnetic field used in the invention and being controllable can be generated by means of current as well as by permanent magnets such as electromagnetic field/electromagnet.
The operation of the invention has been demonstrated by preliminary proof studies with fish sperm DNA using the new platform related to the invention. In the purification step, magnetic microparticles were charged in the microchannel and the microfluidic chip was placed on the magnetic platform. The adsorption liquid with pH 6.5 was passed through the channel for 2 minutes and the surface of the magnetic microparticles was brought to suitable conditions for DNA adsorption. Then, 10pl of approximately 200-300 base pair fish sperm DNA was passed through the microchannel and sample collected at the outlet. The amount of DNA retained by the magnetic particles in the microchannel was calculated from this collected sample. DNA retained on the magnetic particles by using the new magnetic platform was recovered by desorption liquid with pH 8.5 and analyzed. All treatments lasted an average of 20 minutes in total, and 10-15 pg of DNA was retrieved from the system with new platform per 1 mg particle.
Claims
1. A method for retention/purification/enrichment of biological and/or chemical samples, characterized by retention/purification/enrichment of biological and/or chemical samples by increasing interaction surface of sample solution by means of the fact that magnetic particles located inside microchannels and being under the influence of magnetic field are in the same or reverse direction as flow direction of sample solution passed through said microchannels, or are suspended during flow.
2. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , characterized in that the magnetic particles flow through the microchannel together with the sample solution passing through the microchannel, they can also be guided by the adjustable magnetic field with the contribution of magnetic sensitivity, size, channel form, flow rate factors on the other hand.
3. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , wherein magnetic particles located inside microchannels and customized according to the sample solution.
4. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 3, wherein the customized magnetic particles are paramagnetic or diamagnetic particles or mixture thereof.
5. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , characterized in that the magnetic field is formed by using electric current or permanent magnets such as electromagnetic field/electromagnet.
6. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , wherein the microchannels which have circular/spiral, flat or different geometrical shapes.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2104157.9A GB2591406B (en) | 2018-09-05 | 2019-09-05 | Enrichment of samples inside the microchannels by using magnetic particles |
| DE112019004459.1T DE112019004459T5 (en) | 2018-09-05 | 2019-09-05 | ENRICHMENT OF SAMPLES BY MEANS OF MAGNETIC PARTICLES IN MICROCHANNELS |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2018/12647 | 2018-09-05 | ||
| TR201812647 | 2018-09-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2020050809A2 true WO2020050809A2 (en) | 2020-03-12 |
| WO2020050809A3 WO2020050809A3 (en) | 2020-04-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| PCT/TR2019/050728 WO2020050809A2 (en) | 2018-09-05 | 2019-09-05 | Enrichment of samples inside the microchannels by using magnetic particles |
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| Country | Link |
|---|---|
| DE (1) | DE112019004459T5 (en) |
| GB (1) | GB2591406B (en) |
| WO (1) | WO2020050809A2 (en) |
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| DE102022210704A1 (en) | 2022-10-11 | 2024-04-11 | Robert Bosch Gesellschaft mit beschränkter Haftung | Cleaning cartridge for a microfluidic device, microfluidic device and method for cleaning the microfluidic device |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4643822A (en) | 1985-02-28 | 1987-02-17 | The Secretary Of State For Trade And Industry In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Method of separation of material from material mixtures |
| JP3115501B2 (en) | 1994-06-15 | 2000-12-11 | プレシジョン・システム・サイエンス株式会社 | Method for controlling desorption of magnetic material using dispenser and various devices processed by this method |
| DE19500660B4 (en) | 1994-12-10 | 2007-12-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Device and method for manipulating microscopic particles and their use |
| US5973138A (en) | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
| JP3711988B2 (en) | 2003-05-12 | 2005-11-02 | 株式会社日立製作所 | Fine particle array analysis system, fine particle array kit, and chemical analysis method |
| US8162149B1 (en) * | 2009-01-21 | 2012-04-24 | Sandia Corporation | Particle sorter comprising a fluid displacer in a closed-loop fluid circuit |
| EP2678429B1 (en) * | 2011-02-21 | 2018-04-18 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US10130949B2 (en) * | 2011-09-19 | 2018-11-20 | Sorbonne Universite, Centre National De La Recherche Scientifique | Microfluidic system |
| US9709469B2 (en) * | 2011-11-11 | 2017-07-18 | The Regents Of The University Of California | Valveless microfluidic device |
| CN103820304B (en) * | 2014-02-25 | 2015-09-16 | 苏州天隆生物科技有限公司 | Microfluid 3 D electromagnetic for nucleic acid purification excites evenly mixing device |
| WO2017136751A1 (en) * | 2016-02-05 | 2017-08-10 | The Broad Institute Inc. | Multi-stage, multiplexed target isolation and processing from heterogeneous populations |
-
2019
- 2019-09-05 DE DE112019004459.1T patent/DE112019004459T5/en active Pending
- 2019-09-05 WO PCT/TR2019/050728 patent/WO2020050809A2/en active Application Filing
- 2019-09-05 GB GB2104157.9A patent/GB2591406B/en active Active
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| Publication number | Publication date |
|---|---|
| GB202104157D0 (en) | 2021-05-05 |
| GB2591406A (en) | 2021-07-28 |
| WO2020050809A3 (en) | 2020-04-30 |
| GB2591406B (en) | 2023-03-01 |
| DE112019004459T5 (en) | 2021-07-15 |
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