GB2591406A - Enrichment of samples inside the microchannels by using magnetic particles - Google Patents

Enrichment of samples inside the microchannels by using magnetic particles Download PDF

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Publication number
GB2591406A
GB2591406A GB2104157.9A GB202104157A GB2591406A GB 2591406 A GB2591406 A GB 2591406A GB 202104157 A GB202104157 A GB 202104157A GB 2591406 A GB2591406 A GB 2591406A
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GB
United Kingdom
Prior art keywords
enrichment
biological
retention
purification
chemical samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB2104157.9A
Other versions
GB202104157D0 (en
GB2591406B (en
Inventor
Hatipoglu Utku
Cetin Barbaros
Kibar Gunes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adana Bilim Ve Teknoloji Univ
Ihsan Dogramaci Bilkent Universitesity
Original Assignee
Adana Bilim Ve Teknoloji Univ
Ihsan Dogramaci Bilkent Universitesity
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Adana Bilim Ve Teknoloji Univ, Ihsan Dogramaci Bilkent Universitesity filed Critical Adana Bilim Ve Teknoloji Univ
Publication of GB202104157D0 publication Critical patent/GB202104157D0/en
Publication of GB2591406A publication Critical patent/GB2591406A/en
Application granted granted Critical
Publication of GB2591406B publication Critical patent/GB2591406B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

This invention relates to purification method for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, wherein it is particularly microfluidic based biological and/or chemical sample purification method by customized magnetic particles.

Claims (6)

1. A method for retention/purification/enrichment of biological and/or chemical samples, characterized by retention/purification/enrichment of biological and/or chemical samples by increasing interaction surface of sample solution by means of the fact that magnetic particles located inside microchannels and being under the influence of magnetic field are in the same or reverse direction as flow direction of sample solution passed through said microchannels, or are suspended during flow.
2. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , characterized in that the magnetic particles flow through the microchannel together with the sample solution passing through the microchannel, they can also be guided by the adjustable magnetic field with the contribution of magnetic sensitivity, size, channel form, flow rate factors on the other hand.
3. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , wherein magnetic particles located inside microchannels and customized according to the sample solution.
4. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 3, wherein the customized magnetic particles are paramagnetic or diamagnetic particles or mixture thereof.
5. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , characterized in that the magnetic field is formed by using electric current or permanent magnets such as electromagnetic field/electromagnet.
6. A method for retention/purification/enrichment of biological and/or chemical samples according to Claim 1 , wherein the microchannels which have circular/spiral, flat or different geometrical shapes.
GB2104157.9A 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles Active GB2591406B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR201812647 2018-09-05
PCT/TR2019/050728 WO2020050809A2 (en) 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles

Publications (3)

Publication Number Publication Date
GB202104157D0 GB202104157D0 (en) 2021-05-05
GB2591406A true GB2591406A (en) 2021-07-28
GB2591406B GB2591406B (en) 2023-03-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
GB2104157.9A Active GB2591406B (en) 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles

Country Status (3)

Country Link
DE (1) DE112019004459T5 (en)
GB (1) GB2591406B (en)
WO (1) WO2020050809A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022210704A1 (en) 2022-10-11 2024-04-11 Robert Bosch Gesellschaft mit beschränkter Haftung Cleaning cartridge for a microfluidic device, microfluidic device and method for cleaning the microfluidic device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8162149B1 (en) * 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
WO2013041983A1 (en) * 2011-09-19 2013-03-28 Centre National De La Recherche Scientifique Microfluidic system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4643822A (en) 1985-02-28 1987-02-17 The Secretary Of State For Trade And Industry In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method of separation of material from material mixtures
JP3115501B2 (en) 1994-06-15 2000-12-11 プレシジョン・システム・サイエンス株式会社 Method for controlling desorption of magnetic material using dispenser and various devices processed by this method
DE19500660B4 (en) 1994-12-10 2007-12-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for manipulating microscopic particles and their use
US5973138A (en) 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
JP3711988B2 (en) 2003-05-12 2005-11-02 株式会社日立製作所 Fine particle array analysis system, fine particle array kit, and chemical analysis method
US8722329B2 (en) * 2011-02-21 2014-05-13 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
US9709469B2 (en) * 2011-11-11 2017-07-18 The Regents Of The University Of California Valveless microfluidic device
CN103820304B (en) * 2014-02-25 2015-09-16 苏州天隆生物科技有限公司 Microfluid 3 D electromagnetic for nucleic acid purification excites evenly mixing device
US10962527B2 (en) * 2016-02-05 2021-03-30 The Broad Institute, Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8162149B1 (en) * 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
WO2013041983A1 (en) * 2011-09-19 2013-03-28 Centre National De La Recherche Scientifique Microfluidic system

Also Published As

Publication number Publication date
GB202104157D0 (en) 2021-05-05
DE112019004459T5 (en) 2021-07-15
WO2020050809A3 (en) 2020-04-30
WO2020050809A2 (en) 2020-03-12
GB2591406B (en) 2023-03-01

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