AU2012220825B2 - Microfluidic device-based nucleic acid purification method - Google Patents
Microfluidic device-based nucleic acid purification method Download PDFInfo
- Publication number
- AU2012220825B2 AU2012220825B2 AU2012220825A AU2012220825A AU2012220825B2 AU 2012220825 B2 AU2012220825 B2 AU 2012220825B2 AU 2012220825 A AU2012220825 A AU 2012220825A AU 2012220825 A AU2012220825 A AU 2012220825A AU 2012220825 B2 AU2012220825 B2 AU 2012220825B2
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- solution
- purification
- beads
- microfluidic device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161444952P | 2011-02-21 | 2011-02-21 | |
| US61/444,952 | 2011-02-21 | ||
| PCT/US2012/025861 WO2012115926A2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012220825A1 AU2012220825A1 (en) | 2013-08-08 |
| AU2012220825B2 true AU2012220825B2 (en) | 2015-12-17 |
Family
ID=46653044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012220825A Ceased AU2012220825B2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8722329B2 (https=) |
| EP (1) | EP2678429B1 (https=) |
| JP (1) | JP6113083B2 (https=) |
| AU (1) | AU2012220825B2 (https=) |
| WO (1) | WO2012115926A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2678429B1 (en) * | 2011-02-21 | 2018-04-18 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| US11427815B2 (en) | 2015-12-28 | 2022-08-30 | Koninklijke Philips N.V. | Nucleic acid purification system using a single wash and elution buffer solution |
| DE102017206155A1 (de) | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| EP3611508B1 (en) * | 2017-05-16 | 2022-08-03 | SK Telecom Co., Ltd. | Nucleic acid analysis apparatus using catridge |
| GB2591406B (en) * | 2018-09-05 | 2023-03-01 | Ihsan Dogramaci Bilkent Univ | Enrichment of samples inside the microchannels by using magnetic particles |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN115820412B (zh) * | 2022-11-17 | 2025-12-02 | 北京昌平实验室 | 一种从复杂样本中特异检测核酸的微流控卡盒及方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090253181A1 (en) * | 2008-01-22 | 2009-10-08 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3788082T2 (de) * | 1986-04-11 | 1994-06-09 | Applied Biosystems | Automatisches Gerät zum Extrahieren von Nukleinsäuren. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US6037465A (en) | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| WO1999040098A1 (de) | 1998-02-04 | 1999-08-12 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| WO2003057910A2 (en) | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Use of silica material in an amplification reaction |
| EP1388588B1 (en) * | 2002-07-29 | 2008-09-17 | JSR Corporation | Nucleic acid-separating method and nucleic acid-extracting reagent |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| EP2261650A3 (en) * | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| JP4980349B2 (ja) | 2005-06-23 | 2012-07-18 | シーメンス メディカル ソリューションズ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 閉鎖された超薄シリカ層を有する磁性粒子、その製造方法および使用 |
| US7976795B2 (en) * | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| WO2008116182A1 (en) | 2007-03-21 | 2008-09-25 | Ibis Biosciences, Inc. | Reagents for nucleic acid purification |
| JP5523327B2 (ja) * | 2007-10-12 | 2014-06-18 | レオニックス，インコーポレイテッド | 統合型マイクロ流体デバイスおよび方法 |
| EP2394152B1 (en) * | 2009-02-03 | 2019-05-01 | ANDE Corporation | Nucleic acid purification |
| US20120164036A1 (en) * | 2009-07-21 | 2012-06-28 | Seth Stern | Microfluidic devices and uses thereof |
| EP2678429B1 (en) * | 2011-02-21 | 2018-04-18 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
-
2012
- 2012-02-21 EP EP12749025.8A patent/EP2678429B1/en active Active
- 2012-02-21 AU AU2012220825A patent/AU2012220825B2/en not_active Ceased
- 2012-02-21 US US13/400,857 patent/US8722329B2/en active Active
- 2012-02-21 JP JP2013554672A patent/JP6113083B2/ja not_active Expired - Fee Related
- 2012-02-21 WO PCT/US2012/025861 patent/WO2012115926A2/en not_active Ceased
-
2014
- 2014-03-26 US US14/226,204 patent/US9169479B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090253181A1 (en) * | 2008-01-22 | 2009-10-08 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2678429A2 (en) | 2014-01-01 |
| US9169479B2 (en) | 2015-10-27 |
| WO2012115926A3 (en) | 2013-03-21 |
| US20120214168A1 (en) | 2012-08-23 |
| JP6113083B2 (ja) | 2017-04-19 |
| US8722329B2 (en) | 2014-05-13 |
| EP2678429A4 (en) | 2014-10-29 |
| EP2678429B1 (en) | 2018-04-18 |
| JP2014507155A (ja) | 2014-03-27 |
| WO2012115926A2 (en) | 2012-08-30 |
| US20140206009A1 (en) | 2014-07-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |