JP2001503249A - 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター - Google Patents

選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター

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Publication number
JP2001503249A
JP2001503249A JP51028798A JP51028798A JP2001503249A JP 2001503249 A JP2001503249 A JP 2001503249A JP 51028798 A JP51028798 A JP 51028798A JP 51028798 A JP51028798 A JP 51028798A JP 2001503249 A JP2001503249 A JP 2001503249A
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JP
Japan
Prior art keywords
promoter
sequence
group
artificial promoter
stranded dna
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP51028798A
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English (en)
Japanese (ja)
Other versions
JP2001503249A5 (https=
Inventor
ハンメル，カリン
ルーダル イェンセン，ペテル
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Individual
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Individual
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Application filed by Individual filed Critical Individual
Publication of JP2001503249A publication Critical patent/JP2001503249A/ja
Publication of JP2001503249A5 publication Critical patent/JP2001503249A5/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP51028798A 1996-08-23 1997-08-25 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター Withdrawn JP2001503249A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK88696 1996-08-23
DK0886/96 1996-08-23
PCT/DK1997/000342 WO1998007846A1 (en) 1996-08-23 1997-08-25 Artificial promoter libraries for selected organisms and promoters derived from such libraries

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2008232486A Division JP4469005B2 (ja) 1996-08-23 2008-09-10 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター

Publications (2)

Publication Number Publication Date
JP2001503249A true JP2001503249A (ja) 2001-03-13
JP2001503249A5 JP2001503249A5 (https=) 2005-04-07

Family

ID=8098645

Family Applications (2)

Application Number Title Priority Date Filing Date
JP51028798A Withdrawn JP2001503249A (ja) 1996-08-23 1997-08-25 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター
JP2008232486A Expired - Lifetime JP4469005B2 (ja) 1996-08-23 2008-09-10 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2008232486A Expired - Lifetime JP4469005B2 (ja) 1996-08-23 2008-09-10 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター

Country Status (8)

Country Link
US (1) US7199233B1 (https=)
EP (2) EP2034021A1 (https=)
JP (2) JP2001503249A (https=)
CN (1) CN1243827C (https=)
AU (1) AU3938397A (https=)
DE (1) DE69739121D1 (https=)
DK (1) DK0934406T3 (https=)
WO (1) WO1998007846A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009279007A (ja) * 2002-04-22 2009-12-03 E I Du Pont De Nemours & Co 遺伝子工学のためのプロモーターおよびプラスミドシステム
JP2022506156A (ja) * 2018-11-05 2022-01-17 ジェネンテック， インコーポレイテッド 原核宿主細胞における２鎖タンパク質の産生方法

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8574898A (en) 1997-07-14 1999-02-08 Valentis, Inc. Method for the identification of synthetic cell- or tissue-specific transcriptional regulatory regions
US6475183B1 (en) 1998-06-03 2002-11-05 Baxter International Inc. Direct dual filling device for sealing agents
SK7702000A3 (en) 1998-09-25 2000-10-09 Ajinomoto Kk Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity
US6555674B2 (en) 2000-08-09 2003-04-29 Nsgene A/S JeT promoter
DE60143959D1 (de) 2000-08-25 2011-03-10 Basf Plant Science Gmbh En kodieren
EP1400593A4 (en) * 2001-06-06 2005-04-06 Takeda Pharmaceutical NEW PROMOTER
EP1995319B1 (en) 2002-04-10 2011-08-31 Novozymes A/S Bacillus licheniformis mutant host cell
EP1696035A3 (en) 2002-04-10 2008-04-02 Novozymes A/S Improved bacillus host cell
US6644365B1 (en) 2002-04-19 2003-11-11 Baxter International, Inc. Tilting direct dual filling device
DK1501947T3 (da) * 2002-04-22 2008-11-17 Genencor Int Fremgangsmåde til at skabe et bibliotek af bakterielle kloner med varierende niveauer af genekspression
JP2006513692A (ja) * 2002-04-22 2006-04-27 ジェネンコー・インターナショナル・インク 様々なレベルの遺伝子発現を生じる修飾プロモーターを作成する方法
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
US20060121581A1 (en) 2002-10-04 2006-06-08 Cervin Marguerite A Production of bacterial strains cross reference to related applications
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
BRPI0407966A (pt) 2003-03-12 2006-03-07 Ajinomoto Kk método para obter uma bactéria, bactéria produzindo l-aminoácido, e, método para produzir um l-aminoácido
US8110672B2 (en) 2005-04-27 2012-02-07 Massachusetts Institute Of Technology Promoter engineering and genetic control
US20070294785A1 (en) * 2006-06-13 2007-12-20 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008100353A2 (en) 2006-11-29 2008-08-21 Athenix Corporation Improved grg23 epsp synthases: compositions and methods of use
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2311961A1 (en) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Model-guided random assembly PCR for the synthesis of eukaryotic promoters
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN102286517B (zh) * 2011-06-10 2016-08-03 中国科学院天津工业生物技术研究所 利用人工调控元件及其文库调控微生物染色体上基因表达强度的方法
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
EP2573172A1 (en) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Means and methods for rhamnolipid production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
WO2014159850A2 (en) 2013-03-14 2014-10-02 Codexis, Inc. Low-phosphate repressible promoter
EP3320088A1 (en) 2015-07-08 2018-05-16 Rheinisch-Westfälische Technische Hochschule Aachen (RWTH) Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria
CN105296486B (zh) * 2015-11-18 2019-04-26 湖北大学 一种启动子文库构建方法和强启动子
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) * 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN105603537A (zh) * 2016-03-11 2016-05-25 南京工业大学 一种启动子文库的构建方法及其应用
KR20200026881A (ko) * 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
CN108118058B (zh) 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
WO2021126961A1 (en) * 2019-12-16 2021-06-24 Ginkgo Bioworks, Inc. Enhanced production of histidine, purine pathway metabolites, and plasmid dna

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215550D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
AU6776194A (en) * 1993-04-28 1994-11-21 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009279007A (ja) * 2002-04-22 2009-12-03 E I Du Pont De Nemours & Co 遺伝子工学のためのプロモーターおよびプラスミドシステム
JP2022506156A (ja) * 2018-11-05 2022-01-17 ジェネンテック， インコーポレイテッド 原核宿主細胞における２鎖タンパク質の産生方法
JP7620546B2 (ja) 2018-11-05 2025-01-23 ジェネンテック， インコーポレイテッド 原核宿主細胞における２鎖タンパク質の産生方法
US12291712B2 (en) 2018-11-05 2025-05-06 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells

Also Published As

Publication number Publication date
JP4469005B2 (ja) 2010-05-26
JP2009022293A (ja) 2009-02-05
EP0934406A2 (en) 1999-08-11
DE69739121D1 (de) 2009-01-02
WO1998007846A1 (en) 1998-02-26
AU3938397A (en) 1998-03-06
EP2034021A1 (en) 2009-03-11
EP0934406B1 (en) 2008-11-19
CN1233287A (zh) 1999-10-27
US7199233B1 (en) 2007-04-03
DK0934406T3 (da) 2009-01-26
CN1243827C (zh) 2006-03-01

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