CN1243827C - 选定生物体的人工启动子文库和得自这种文库的启动子 - Google Patents

选定生物体的人工启动子文库和得自这种文库的启动子 Download PDF

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CN1243827C
CN1243827C CNB971987033A CN97198703A CN1243827C CN 1243827 C CN1243827 C CN 1243827C CN B971987033 A CNB971987033 A CN B971987033A CN 97198703 A CN97198703 A CN 97198703A CN 1243827 C CN1243827 C CN 1243827C
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sequence
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library
promotor
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CN1233287A (zh
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彼得·鲁戴尔·简森
卡林·哈默
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB971987033A 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子 Expired - Lifetime CN1243827C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK88696 1996-08-23
DK0886/96 1996-08-23

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Publication Number Publication Date
CN1233287A CN1233287A (zh) 1999-10-27
CN1243827C true CN1243827C (zh) 2006-03-01

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ID=8098645

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CNB971987033A Expired - Lifetime CN1243827C (zh) 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子

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Country Link
US (1) US7199233B1 (https=)
EP (2) EP2034021A1 (https=)
JP (2) JP2001503249A (https=)
CN (1) CN1243827C (https=)
AU (1) AU3938397A (https=)
DE (1) DE69739121D1 (https=)
DK (1) DK0934406T3 (https=)
WO (1) WO1998007846A1 (https=)

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EP1995319B1 (en) 2002-04-10 2011-08-31 Novozymes A/S Bacillus licheniformis mutant host cell
EP1696035A3 (en) 2002-04-10 2008-04-02 Novozymes A/S Improved bacillus host cell
US6644365B1 (en) 2002-04-19 2003-11-11 Baxter International, Inc. Tilting direct dual filling device
DK1501947T3 (da) * 2002-04-22 2008-11-17 Genencor Int Fremgangsmåde til at skabe et bibliotek af bakterielle kloner med varierende niveauer af genekspression
JP2006513692A (ja) * 2002-04-22 2006-04-27 ジェネンコー・インターナショナル・インク 様々なレベルの遺伝子発現を生じる修飾プロモーターを作成する方法
EP2374879A3 (en) * 2002-04-22 2012-01-11 E. I. du Pont de Nemours and Company Promoter and plasmid system for genetic engineering
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
US20060121581A1 (en) 2002-10-04 2006-06-08 Cervin Marguerite A Production of bacterial strains cross reference to related applications
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
BRPI0407966A (pt) 2003-03-12 2006-03-07 Ajinomoto Kk método para obter uma bactéria, bactéria produzindo l-aminoácido, e, método para produzir um l-aminoácido
US8110672B2 (en) 2005-04-27 2012-02-07 Massachusetts Institute Of Technology Promoter engineering and genetic control
US20070294785A1 (en) * 2006-06-13 2007-12-20 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008100353A2 (en) 2006-11-29 2008-08-21 Athenix Corporation Improved grg23 epsp synthases: compositions and methods of use
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2311961A1 (en) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Model-guided random assembly PCR for the synthesis of eukaryotic promoters
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN102286517B (zh) * 2011-06-10 2016-08-03 中国科学院天津工业生物技术研究所 利用人工调控元件及其文库调控微生物染色体上基因表达强度的方法
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
EP2573172A1 (en) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Means and methods for rhamnolipid production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
WO2014159850A2 (en) 2013-03-14 2014-10-02 Codexis, Inc. Low-phosphate repressible promoter
EP3320088A1 (en) 2015-07-08 2018-05-16 Rheinisch-Westfälische Technische Hochschule Aachen (RWTH) Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria
CN105296486B (zh) * 2015-11-18 2019-04-26 湖北大学 一种启动子文库构建方法和强启动子
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) * 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN105603537A (zh) * 2016-03-11 2016-05-25 南京工业大学 一种启动子文库的构建方法及其应用
KR20200026881A (ko) * 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
CN108118058B (zh) 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
EP3877407B1 (en) * 2018-11-05 2026-03-11 F. Hoffmann-La Roche AG Methods of producing two chain proteins in prokaryotic host cells
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
WO2021126961A1 (en) * 2019-12-16 2021-06-24 Ginkgo Bioworks, Inc. Enhanced production of histidine, purine pathway metabolites, and plasmid dna

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AU6776194A (en) * 1993-04-28 1994-11-21 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking

Also Published As

Publication number Publication date
JP4469005B2 (ja) 2010-05-26
JP2009022293A (ja) 2009-02-05
EP0934406A2 (en) 1999-08-11
DE69739121D1 (de) 2009-01-02
WO1998007846A1 (en) 1998-02-26
AU3938397A (en) 1998-03-06
EP2034021A1 (en) 2009-03-11
EP0934406B1 (en) 2008-11-19
JP2001503249A (ja) 2001-03-13
CN1233287A (zh) 1999-10-27
US7199233B1 (en) 2007-04-03
DK0934406T3 (da) 2009-01-26

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