DE69739121D1 - Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren - Google Patents

Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren

Info

Publication number
DE69739121D1
DE69739121D1 DE69739121T DE69739121T DE69739121D1 DE 69739121 D1 DE69739121 D1 DE 69739121D1 DE 69739121 T DE69739121 T DE 69739121T DE 69739121 T DE69739121 T DE 69739121T DE 69739121 D1 DE69739121 D1 DE 69739121D1
Authority
DE
Germany
Prior art keywords
promotive
libraries
promoters
artificial
selected organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739121T
Other languages
German (de)
English (en)
Inventor
Peter Ruhdal Jensen
Karin Hammer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69739121D1 publication Critical patent/DE69739121D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69739121T 1996-08-23 1997-08-25 Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren Expired - Lifetime DE69739121D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK88696 1996-08-23
PCT/DK1997/000342 WO1998007846A1 (en) 1996-08-23 1997-08-25 Artificial promoter libraries for selected organisms and promoters derived from such libraries

Publications (1)

Publication Number Publication Date
DE69739121D1 true DE69739121D1 (de) 2009-01-02

Family

ID=8098645

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69739121T Expired - Lifetime DE69739121D1 (de) 1996-08-23 1997-08-25 Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren

Country Status (8)

Country Link
US (1) US7199233B1 (https=)
EP (2) EP2034021A1 (https=)
JP (2) JP2001503249A (https=)
CN (1) CN1243827C (https=)
AU (1) AU3938397A (https=)
DE (1) DE69739121D1 (https=)
DK (1) DK0934406T3 (https=)
WO (1) WO1998007846A1 (https=)

Families Citing this family (48)

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EP1003911A1 (en) 1997-07-14 2000-05-31 Valentis Inc. Method for the identification of synthetic cell- or tissue-specific transcriptional regulatory regions
US6475183B1 (en) 1998-06-03 2002-11-05 Baxter International Inc. Direct dual filling device for sealing agents
WO2000018935A1 (en) 1998-09-25 2000-04-06 Ajinomoto Co.,Inc. Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium
US6555674B2 (en) 2000-08-09 2003-04-29 Nsgene A/S JeT promoter
EP1349946B1 (en) 2000-08-25 2011-01-26 BASF Plant Science GmbH Plant polynucleotides encoding prenyl proteases
US20040166566A1 (en) * 2001-06-06 2004-08-26 Takashi Ito Novel promoter
US20050176097A1 (en) 2002-04-10 2005-08-11 Andersen Jens T. Bacillus host cell
ATE399869T1 (de) 2002-04-10 2008-07-15 Novozymes As Verbesserte bacillus-wirtszelle
US6644365B1 (en) 2002-04-19 2003-11-11 Baxter International, Inc. Tilting direct dual filling device
KR20040104581A (ko) * 2002-04-22 2004-12-10 이 아이 듀폰 디 네모아 앤드 캄파니 유전공학용 프로모터 및 플라스미드 시스템
CA2830864C (en) * 2002-04-22 2017-03-21 Genencor International, Inc. Methods of creating modified promoters resulting in varying levels of gene expression
DK1501947T3 (da) * 2002-04-22 2008-11-17 Genencor Int Fremgangsmåde til at skabe et bibliotek af bakterielle kloner med varierende niveauer af genekspression
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
DK1546304T3 (da) 2002-10-04 2013-08-05 Danisco Us Inc Forbedret produktion af bakteriestammer
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
US20060166206A1 (en) * 2002-11-15 2006-07-27 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
JP4821606B2 (ja) 2003-03-12 2011-11-24 味の素株式会社 効率的にＬ−グルタミン酸を生産するためのプロモーター変異による細菌のｓｕｃＡＢ発現の最適化
WO2006116400A2 (en) 2005-04-27 2006-11-02 Massachusetts Institute Of Technology Promoter engineering and genetic control
WO2007146975A2 (en) * 2006-06-13 2007-12-21 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
CN101600800B (zh) 2006-11-29 2013-08-28 埃塞尼克斯公司 改良的grg23 epsp合酶：组合物和使用方法
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
JP5659143B2 (ja) 2008-03-28 2015-01-28 ダニスコ・ユーエス・インク 細菌性細胞中の遺伝子座を増幅する方法
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2311961A1 (en) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Model-guided random assembly PCR for the synthesis of eukaryotic promoters
US20120315670A1 (en) 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
CN102286517B (zh) * 2011-06-10 2016-08-03 中国科学院天津工业生物技术研究所 利用人工调控元件及其文库调控微生物染色体上基因表达强度的方法
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
EP2573172A1 (en) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Means and methods for rhamnolipid production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN111876409A (zh) 2012-04-24 2020-11-03 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
EP2970869A4 (en) 2013-03-14 2016-08-03 Codexis Inc REPRESSIBLE PROMOTER WITH LOW PHOSPHATE CONTENT
BR112018000257A2 (pt) 2015-07-08 2018-09-04 Rheinisch Westfaelische Technische Hochschule Aachen Rwth célula hospedeira, método para produzir um haa, uso de uma célula hospedeira, método para produzir uma célula hospedeira, célula, preparação de haa, método para produzir uma composição de ácido graxo e composição de ácido graxo
CN105296486B (zh) * 2015-11-18 2019-04-26 湖北大学 一种启动子文库构建方法和强启动子
US9988624B2 (en) * 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) * 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
CN105603537A (zh) * 2016-03-11 2016-05-25 南京工业大学 一种启动子文库的构建方法及其应用
CA3064777A1 (en) * 2017-06-07 2018-12-13 Zach Serber Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CN108118058B (zh) 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
WO2020096959A1 (en) 2018-11-05 2020-05-14 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
EP4077650A4 (en) * 2019-12-16 2024-01-17 Ginkgo Bioworks, Inc. ENHANCED PRODUCTION OF HISTIDINE, PURINE PATHWAY METABOLITES, AND PLASMID DNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215550D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
WO1994025609A1 (en) * 1993-04-28 1994-11-10 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking

Also Published As

Publication number Publication date
JP2001503249A (ja) 2001-03-13
EP2034021A1 (en) 2009-03-11
DK0934406T3 (da) 2009-01-26
EP0934406A2 (en) 1999-08-11
US7199233B1 (en) 2007-04-03
EP0934406B1 (en) 2008-11-19
CN1243827C (zh) 2006-03-01
JP4469005B2 (ja) 2010-05-26
AU3938397A (en) 1998-03-06
WO1998007846A1 (en) 1998-02-26
CN1233287A (zh) 1999-10-27
JP2009022293A (ja) 2009-02-05

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