IL158000A0 - Evolving new molecular function - Google Patents

Evolving new molecular function

Info

Publication number
IL158000A0
IL158000A0 IL15800002A IL15800002A IL158000A0 IL 158000 A0 IL158000 A0 IL 158000A0 IL 15800002 A IL15800002 A IL 15800002A IL 15800002 A IL15800002 A IL 15800002A IL 158000 A0 IL158000 A0 IL 158000A0
Authority
IL
Israel
Prior art keywords
molecules
amplification
natural
nucleic acid
selection
Prior art date
Application number
IL15800002A
Other languages
English (en)
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27493169&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL158000(A0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Publication of IL158000A0 publication Critical patent/IL158000A0/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Steroid Compounds (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
IL15800002A 2001-03-19 2002-03-19 Evolving new molecular function IL158000A0 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27708101P 2001-03-19 2001-03-19
US27709401P 2001-03-19 2001-03-19
US30669101P 2001-07-20 2001-07-20
US10/101,030 US7070928B2 (en) 2001-03-19 2002-03-19 Evolving new molecular function
PCT/US2002/008546 WO2002074929A2 (en) 2001-03-19 2002-03-19 Evolving new molecular function

Publications (1)

Publication Number Publication Date
IL158000A0 true IL158000A0 (en) 2004-03-28

Family

ID=27493169

Family Applications (4)

Application Number Title Priority Date Filing Date
IL15800002A IL158000A0 (en) 2001-03-19 2002-03-19 Evolving new molecular function
IL158000A IL158000A (en) 2001-03-19 2003-09-18 Methods for preparation of nucleic acid in synthesis in molds
IL206585A IL206585A (en) 2001-03-19 2010-06-23 Methods for preparation of nucleic acid in synthesis in molds
IL206586A IL206586A (en) 2001-03-19 2010-06-23 A method for the formation of small molecules by patterned synthesis of nucleic acid

Family Applications After (3)

Application Number Title Priority Date Filing Date
IL158000A IL158000A (en) 2001-03-19 2003-09-18 Methods for preparation of nucleic acid in synthesis in molds
IL206585A IL206585A (en) 2001-03-19 2010-06-23 Methods for preparation of nucleic acid in synthesis in molds
IL206586A IL206586A (en) 2001-03-19 2010-06-23 A method for the formation of small molecules by patterned synthesis of nucleic acid

Country Status (12)

Country Link
US (7) US7070928B2 (da)
EP (3) EP2332896A3 (da)
JP (4) JP4128453B2 (da)
AT (1) ATE335754T1 (da)
AU (2) AU2002306777C1 (da)
CA (1) CA2441820C (da)
DE (1) DE60213826T3 (da)
DK (1) DK1423400T4 (da)
ES (1) ES2271306T5 (da)
IL (4) IL158000A0 (da)
PT (1) PT1423400E (da)
WO (1) WO2002074929A2 (da)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
DK1423400T4 (da) * 2001-03-19 2013-09-02 Harvard College Evolution af hidtil ukendt molekylfunktion
CN1539014A (zh) * 2001-06-20 2004-10-20 ŦΤ¬ɭ��˾ 模控分子和使用这种分子的方法
WO2002102820A1 (en) * 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) * 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
DE60231436D1 (de) 2002-03-08 2009-04-16 Eidgenoess Tech Hochschule Kodierte, selbst-assemblierende chemische bibliotheken (esachel)
US20050176948A1 (en) * 2002-03-15 2005-08-11 Gouliaev Alex H. Building block capable of functional entity transfer to nucleophil
AU2003218629A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
IL163822A0 (en) * 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
US20050221318A1 (en) * 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
US10730906B2 (en) 2002-08-01 2020-08-04 Nuevolutions A/S Multi-step synthesis of templated molecules
WO2004016767A2 (en) * 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
WO2004024929A2 (en) * 2002-09-12 2004-03-25 Nuevolution A/S Proximity-aided synthesis of templated molecules
AU2003266932B2 (en) * 2002-09-27 2009-04-09 Mpm-Holding Aps Spatially encoded polymer matrix
AU2011226815B2 (en) * 2002-10-30 2014-09-25 Nuevolution A/S Enzymatic encoding
PT1558744E (pt) 2002-10-30 2011-09-22 Nuevolution As Codificação enzimática
CA2508726A1 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
ATE450609T1 (de) * 2002-12-19 2009-12-15 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
US20060269920A1 (en) * 2003-02-21 2006-11-30 Nuevolution A/S Method for obtaining structural information about an encoded molecule
ATE424455T1 (de) 2003-03-20 2009-03-15 Nuevolution As Ligationsvermittelnde codierung von kleinen molekülen
US8017323B2 (en) * 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US20050010029A1 (en) * 2003-05-08 2005-01-13 Brown Dennis M. Recombined molecules and preparation thereof
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
WO2004110964A2 (en) * 2003-06-16 2004-12-23 Nuevolution A/S Encoded molecules by translation (emt)
WO2005003778A2 (en) * 2003-07-02 2005-01-13 Nuevolution A/S A method for identifying a synthetic molecule having affinity towards a target
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122103A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
ATE447626T1 (de) 2003-09-18 2009-11-15 Nuevolution As Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen
CA2549386C (en) 2003-12-17 2014-07-29 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
US20090239211A1 (en) * 2004-02-17 2009-09-24 Nuevolution A/S Method For Enrichment Involving Elimination By Mismatch Hybridisation
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
EP1730277B1 (en) 2004-03-22 2009-10-28 Nuevolution A/S Ligational encoding using building block oligonucleotides
ES2641832T3 (es) 2004-05-24 2017-11-14 Ibis Biosciences, Inc. Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
WO2005123959A2 (en) 2004-06-10 2005-12-29 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
KR101411361B1 (ko) 2004-11-08 2014-06-25 비퍼겐 에이피에스 구조적 핵산에 의해 가이드되는 화학적 합성
WO2006053571A2 (en) 2004-11-22 2006-05-26 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US20060205040A1 (en) 2005-03-03 2006-09-14 Rangarajan Sampath Compositions for use in identification of adventitious viruses
CA2605021A1 (en) * 2005-04-13 2007-08-02 Isis Pharmaceuticals, Inc. Compositions for use in identification of adenoviruses
US7968289B2 (en) * 2005-05-03 2011-06-28 Ensemble Therapeutics Corporation Turn over probes and use thereof for nucleic acid detection
KR20080028886A (ko) * 2005-05-26 2008-04-02 앙상블 디스커버리 코포레이션 핵산-템플레이팅된 화학 반응에 의한 생검출
WO2006135654A2 (en) * 2005-06-07 2006-12-21 President And Fellows Of Harvard College Polymer evolution via templated synthesis related applications
SI1910538T1 (sl) 2005-06-09 2011-09-30 Praecis Pharm Inc POSTOPKI ZA SINTEZO KODIRANIH KNJIĹľNIC
WO2006138560A2 (en) * 2005-06-17 2006-12-28 President And Fellows Of Harvard College Nucleic acid-templated chemistry in organic solvents
DE602006013134D1 (de) * 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
WO2007011722A2 (en) 2005-07-15 2007-01-25 President And Fellows Of Harvard College Reaction discovery system
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
DE602005003374T2 (de) * 2005-10-17 2008-09-11 Pls-Design Gmbh Verfahren zur Bestimmung einer unbekannten PNS Seqenz und seine Verwendungen
EP2368868A1 (en) 2005-10-28 2011-09-28 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
DK3305900T3 (da) 2005-12-01 2021-10-25 Nuevolution As Enzymatic encoding methods for efficient synthesis of large libraries
EP2021470A1 (en) 2006-05-03 2009-02-11 Vipergen APS A method for preparing compounds by nucleic acid directed synthesis
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
EP2064348B1 (en) * 2006-09-18 2012-02-08 Ensemble Therapeutics Corporation Receptor family profiling
AU2007314516A1 (en) * 2006-09-28 2008-05-08 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
EP2191268A2 (en) * 2007-07-27 2010-06-02 Ensemble Discovery Corporation Detection assays and use thereof
WO2009134470A2 (en) * 2008-01-18 2009-11-05 The Johns Hopkins University Methods for identifying eubacteria
EP2350031A2 (en) * 2008-08-20 2011-08-03 Ensemble Therapeutics Corporation Macrocyclic compounds for inhibition of tumor necrosis factor alpha
JP2012502911A (ja) * 2008-09-14 2012-02-02 インサイター インコーポレイテッド ナノスケール分子合成
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
WO2010093943A1 (en) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ionization probe assemblies
ES2638324T3 (es) * 2009-02-13 2017-10-19 X-Chem, Inc. Métodos de creación y examen de bibliotecas codificadas por ADN
DK2446033T3 (da) 2009-06-22 2014-01-27 Univ Strasbourg Fremgangsmåde til fremstilling af et addukt
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
EP2454000A4 (en) 2009-07-17 2016-08-10 Ibis Biosciences Inc SYSTEMS FOR IDENTIFYING BIOLOGICAL SUBSTANCES
EP2488656B1 (en) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Multiple displacement amplification
LT2558577T (lt) 2010-04-16 2019-03-12 Nuevolution A/S Bifunkciniai kompleksai ir tokių kompleksų gamybos ir naudojimo būdai
EP2622073B1 (en) 2010-09-27 2018-04-18 Vipergen A method for making an enriched library
CA2835942C (en) * 2011-05-19 2019-01-22 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
AU2012304387B2 (en) 2011-09-07 2017-03-16 X-Chem, Inc. Methods for tagging DNA-encoded libraries
NZ739931A (en) 2012-07-13 2019-08-30 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
WO2014143954A2 (en) * 2013-03-15 2014-09-18 Arizona Board Of Regents On Behalf Of Arizona State University Biosensor microarray compositions and methods
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
GB201322692D0 (en) 2013-12-20 2014-02-05 Philochem Ag Production of encoded chemical libraries
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
EP3218479A1 (en) 2014-11-11 2017-09-20 Nanocore ApS Method for identification of molecules with desired characteristics
WO2016094845A2 (en) * 2014-12-12 2016-06-16 Woolf Tod M Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides
CN114540470A (zh) 2015-04-24 2022-05-27 基纳生物技术有限公司 用于次要等位基因和多态性的鉴定和定量的多重化方法
IL294014B2 (en) 2015-10-23 2024-07-01 Harvard College Nucleobase editors and their uses
CN115820806A (zh) 2016-06-16 2023-03-21 哈斯达克科学公司 寡核苷酸指导和记录的编码探针分子的组合合成
CA3032699A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018081534A1 (en) 2016-10-28 2018-05-03 President And Fellows Of Harvard College Assay for exo-site binding molecules
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation MOLECULES FOR THE VERIFICATION OF OLIGONUCLEOTIDE DIRECTED COMBINATIONAL SYNTHESIS AND METHOD OF MANUFACTURING AND USING THEREOF
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
EP3681637A4 (en) * 2017-09-11 2021-06-02 Synthego Corporation SYSTEM AND PROCEDURE FOR BIOPOLYMER SYNTHESIS
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
US11674136B2 (en) 2018-02-09 2023-06-13 President And Fellows Of Harvard College DNA-templated macrocycle library
EP3899521A4 (en) 2018-12-20 2022-08-24 Sergey N. Krylov BINDER SELECTION USING CAPILLARY ELECTROPHORESIS
SG11202107913VA (en) 2019-01-22 2021-08-30 Vipergen Aps A method for screening of an in vitro display library within a cell
DE112020001342T5 (de) 2019-03-19 2022-01-13 President and Fellows of Harvard College Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
WO2023164224A1 (en) * 2022-02-28 2023-08-31 The Board Of Trustees Of The Leland Stanford Junior University Functionalized nanoclusters and their use in treating bacterial infections

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US277081A (en) 1883-05-08 Scalding napped hats and sizing or felting hat and other fabrics
US4194550A (en) * 1976-01-26 1980-03-25 Insulating Shade (Limited Partnership) Apparatus for insulating against conductive, convective and radiant heat transmission
US4863857A (en) * 1985-03-01 1989-09-05 Board Of Regents, The University Of Texas System Polypeptide complementary to peptides or proteins having an amino acid sequence or nucleotide coding sequence at least partially known
US5506337A (en) * 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4703034A (en) * 1986-04-28 1987-10-27 Merck & Co., Inc. Novel cyclic tetrapeptide
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5162218A (en) * 1988-11-18 1992-11-10 The Regents Of The University Of California Conjugated polypeptides and methods for their preparation
US6680192B1 (en) * 1989-05-16 2004-01-20 Scripps Research Institute Method for producing polymers having a preselected activity
US6291160B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
ES2118066T3 (es) 1989-10-05 1998-09-16 Optein Inc Sintesis y aislamiento, exentos de celulas, de nuevos genes y polipeptidos.
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5723289A (en) * 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US5637682A (en) 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. High-affinity oligonucleotide ligands to the tachykinin substance P
AU8498091A (en) 1990-08-02 1992-03-02 Regents Of The University Of Colorado, The Systematic polypeptide evolution by reverse translation
US20020038000A1 (en) 1990-08-02 2002-03-28 Larry Gold Systematic polypeptide evolution by reverse translation
US5843701A (en) * 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US6140496A (en) * 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5641625A (en) * 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
US5719262A (en) * 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
ATE163308T1 (de) * 1991-08-28 1998-03-15 Boehringer Mannheim Gmbh Primer für matrizenabhängige enzymatische nukleinsäuresynthesen
AU669489B2 (en) * 1991-09-18 1996-06-13 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
ATE293011T1 (de) 1991-11-22 2005-04-15 Affymetrix Inc A Delaware Corp Kombinatorische strategien für die polymersynthese
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) * 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ATE267877T1 (de) 1993-01-07 2004-06-15 Sequenom Inc Dns - sequenzierung durch massenspektronomie
JPH08507926A (ja) 1993-03-19 1996-08-27 シーケノム・インコーポレーテツド エキソヌクレアーゼ分解を介した質量分析法によるｄｎａ配列決定
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5840485A (en) * 1993-05-27 1998-11-24 Selectide Corporation Topologically segregated, encoded solid phase libraries
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5922545A (en) * 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US6127154A (en) * 1994-02-10 2000-10-03 Mosbach; Klaus Methods for direct synthesis of compounds having complementary structure to a desired molecular entity and use thereof
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20030099945A1 (en) * 1994-09-20 2003-05-29 Invenux, Inc. Parallel selex
US6048698A (en) * 1994-09-20 2000-04-11 Nexstar Pharmaceuticals, Inc. Parallel SELEX™
US5597697A (en) * 1994-09-30 1997-01-28 Diamond; Paul Screening assay for inhibitors and activators of RNA and DNA-dependent nucleic acid polymerases
GB9524630D0 (en) * 1994-12-24 1996-01-31 Zeneca Ltd Chemical compounds
US5559000A (en) * 1995-01-18 1996-09-24 The Scripps Research Institute Encoded reaction cassette
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5846839A (en) * 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US5888971A (en) * 1996-02-20 1999-03-30 Ortho Pharmaceutical Corporation, Inc. Macrocyclic peptides useful in the treatment of thrombin related disorders
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5998140A (en) * 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6080826A (en) * 1997-01-06 2000-06-27 California Institute Of Technology Template-directed ring-closing metathesis and ring-opening metathesis polymerization of functionalized dienes
EP2256133B1 (en) * 1997-01-08 2016-12-14 Sigma-Aldrich Co. LLC Bioconjugation of macromolecules
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
US6969584B2 (en) 1997-06-12 2005-11-29 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
DE1051515T1 (de) 1998-01-27 2001-04-19 Cytocell Ltd., Banbury Modifizierte nuklein-säure sonden und dessen verwendungen
US5945325A (en) 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
AU1318400A (en) * 1998-10-19 2000-05-08 Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
EP1208219A1 (en) 1999-04-08 2002-05-29 Pavel Sergeev Synthesis of biologically active compounds in cells
AU7057500A (en) * 1999-08-12 2001-03-13 Maxygen, Inc. Evolution and use of enzymes for combinatorial and medicinal chemistry
EP1212452B1 (en) * 1999-08-27 2013-07-17 Bristol-Myers Squibb Company Methods for encoding and sorting in vitro translated proteins
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
DK1423400T4 (da) * 2001-03-19 2013-09-02 Harvard College Evolution af hidtil ukendt molekylfunktion
CN1539014A (zh) 2001-06-20 2004-10-20 ŦΤ¬ɭ��˾ 模控分子和使用这种分子的方法
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20030143561A1 (en) * 2001-06-20 2003-07-31 Nuevolution A/S Nucleoside derivatives for library preparation
WO2003078445A2 (en) 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c=c double bond upon reaction
DE60231436D1 (de) 2002-03-08 2009-04-16 Eidgenoess Tech Hochschule Kodierte, selbst-assemblierende chemische bibliotheken (esachel)
IL163822A0 (en) 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
US20050221318A1 (en) 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
US20050176948A1 (en) 2002-03-15 2005-08-11 Gouliaev Alex H. Building block capable of functional entity transfer to nucleophil
AU2003218629A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
WO2003082901A2 (en) 2002-03-22 2003-10-09 Emory University Template-driven processes for synthesizing polymers and components related to such processes
EP1520040A2 (en) 2002-06-20 2005-04-06 Nuevolution A/S Microarrays displaying encoded molecules
WO2004016767A2 (en) * 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
US8017323B2 (en) 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
ATE461279T1 (de) * 2005-06-07 2010-04-15 Harvard College Geordnete mehrschrittsynthese mittels nukleinsäurevermittelter chemie
DE602006013134D1 (de) 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
WO2006138560A2 (en) * 2005-06-17 2006-12-28 President And Fellows Of Harvard College Nucleic acid-templated chemistry in organic solvents

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