IE60485B1 - Process for producing biological active plasminogen activator - Google Patents
Process for producing biological active plasminogen activatorInfo
- Publication number
- IE60485B1 IE60485B1 IE150487A IE150487A IE60485B1 IE 60485 B1 IE60485 B1 IE 60485B1 IE 150487 A IE150487 A IE 150487A IE 150487 A IE150487 A IE 150487A IE 60485 B1 IE60485 B1 IE 60485B1
- Authority
- IE
- Ireland
- Prior art keywords
- plasminogen activator
- medium
- type plasminogen
- tissue
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 33
- 102000001938 Plasminogen Activators Human genes 0.000 title abstract description 8
- 108010001014 Plasminogen Activators Proteins 0.000 title abstract description 8
- 229940127126 plasminogen activator Drugs 0.000 title abstract description 8
- 101000801481 Homo sapiens Tissue-type plasminogen activator Proteins 0.000 claims abstract description 31
- 230000001627 detrimental effect Effects 0.000 claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 13
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- 238000012258 culturing Methods 0.000 claims abstract description 5
- 238000011084 recovery Methods 0.000 claims abstract description 5
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- 239000012533 medium component Substances 0.000 claims abstract 2
- 210000004027 cell Anatomy 0.000 claims description 58
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 claims description 19
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 claims description 19
- 230000012010 growth Effects 0.000 claims description 17
- 239000000725 suspension Substances 0.000 claims description 14
- 238000009295 crossflow filtration Methods 0.000 claims description 12
- 239000006285 cell suspension Substances 0.000 claims description 10
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- 239000000047 product Substances 0.000 claims description 5
- 230000008021 deposition Effects 0.000 claims description 4
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- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
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- 108010022394 Threonine synthase Proteins 0.000 claims description 2
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- 238000004519 manufacturing process Methods 0.000 abstract description 22
- 230000007717 exclusion Effects 0.000 abstract 1
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- 239000002609 medium Substances 0.000 description 38
- 239000012190 activator Substances 0.000 description 14
- 108050006955 Tissue-type plasminogen activator Proteins 0.000 description 13
- 102100033571 Tissue-type plasminogen activator Human genes 0.000 description 13
- 210000002966 serum Anatomy 0.000 description 11
- 239000012510 hollow fiber Substances 0.000 description 9
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- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
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- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 3
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- 102000013566 Plasminogen Human genes 0.000 description 3
- 108010051456 Plasminogen Proteins 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
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- -1 huaaan fc-ΡΑ Proteins 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
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- 238000010561 standard procedure Methods 0.000 description 2
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- DSGKWFGEUBCEIE-UHFFFAOYSA-N (2-carbonochloridoylphenyl) acetate Chemical compound CC(=O)OC1=CC=CC=C1C(Cl)=O DSGKWFGEUBCEIE-UHFFFAOYSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
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- 241000699800 Cricetinae Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241000763212 Lype Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000801924 Sena Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000022811 deglycosylation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000010339 dilation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
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- 238000001962 electrophoresis Methods 0.000 description 1
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- 238000000605 extraction Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012090 serum-supplement Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011269 tar Substances 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Diabetes (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87164286A | 1986-06-06 | 1986-06-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE871504L IE871504L (en) | 1987-12-06 |
| IE60485B1 true IE60485B1 (en) | 1994-07-27 |
Family
ID=25357832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE150487A IE60485B1 (en) | 1986-06-06 | 1987-06-05 | Process for producing biological active plasminogen activator |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5053334A (https=) |
| EP (1) | EP0248675B1 (https=) |
| JP (1) | JPS6332484A (https=) |
| KR (1) | KR930002888B1 (https=) |
| AT (1) | ATE75775T1 (https=) |
| AU (1) | AU601984B2 (https=) |
| DD (1) | DD257087A5 (https=) |
| DE (2) | DE3778758D1 (https=) |
| DK (1) | DK175366B1 (https=) |
| ES (1) | ES2032444T3 (https=) |
| FI (1) | FI91886C (https=) |
| FR (1) | FR2599625B1 (https=) |
| GB (1) | GB2191493B (https=) |
| GR (1) | GR3005245T3 (https=) |
| HU (1) | HU205171B (https=) |
| IE (1) | IE60485B1 (https=) |
| IL (1) | IL82746A (https=) |
| NO (1) | NO170595C (https=) |
| NZ (1) | NZ220547A (https=) |
| PT (1) | PT84991B (https=) |
| ZA (1) | ZA874054B (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT84991B (pt) * | 1986-06-06 | 1990-03-08 | Genentech Inc | Processo para a producao de actividador do plasminogenio biologicamente activo |
| JP2576200B2 (ja) * | 1988-03-09 | 1997-01-29 | 味の素株式会社 | 生理活性タンパク質の製造法 |
| EP0338716A3 (en) * | 1988-04-21 | 1990-04-11 | Berlex Laboratories, Inc. | Method and system for the production of non-degraded proteins from mammalian cells |
| DE3829244A1 (de) * | 1988-08-29 | 1990-03-01 | Boehringer Mannheim Gmbh | Verfahren zur herstellung von einkettigem t-pa |
| CA2011548A1 (en) * | 1989-03-07 | 1990-09-07 | Hubertus Stockinger | Process for the production of two-chain t-pa |
| GB2249099B (en) * | 1990-09-26 | 1995-05-03 | Squibb & Sons Inc | Squalene synthetase |
| US5677184A (en) * | 1994-04-19 | 1997-10-14 | Takeda Chemical Industries, Ltd. | CHO cells that express human LH-RH receptor |
| US6077940A (en) * | 1997-12-24 | 2000-06-20 | Genentech, Inc. | Free solution ligand interaction molecular separation method |
| WO2003087292A2 (en) | 2002-04-08 | 2003-10-23 | Millenium Biologix Inc. | Automated tissue engineering system |
| SI2041259T1 (sl) | 2006-07-14 | 2016-04-29 | Dpx Holdings B.V. | Izboljšan postopek kultiviranja celic |
| WO2009023562A2 (en) * | 2007-08-09 | 2009-02-19 | Wyeth | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| JP2013517771A (ja) * | 2010-01-22 | 2013-05-20 | ロンザ ウォーカーズヴィル，インコーポレーテッド | タンジェンシャルフローフィルトレーションを用いた治療用細胞の容量減少および洗浄のための高収率法および装置 |
| US10934519B2 (en) | 2011-07-29 | 2021-03-02 | Global Life Sciences Solutions Usa Llc | Systems, methods and control laws for cell harvesting |
| CN104583386A (zh) * | 2012-08-20 | 2015-04-29 | 泰尔茂比司特公司 | 浓缩穿过细胞生长腔室循环的流体的组分 |
| CA3248436A1 (en) | 2017-09-01 | 2025-02-21 | Lonza Walkersville, Inc. | END-TO-END AUTOMATION OF CELL THERAPY |
| JP7396777B2 (ja) | 2018-09-28 | 2023-12-12 | オクタン バイオテック インコーポレーテッド | 磁気分離 |
| KR20210108406A (ko) | 2018-12-21 | 2021-09-02 | 론자 워커스빌 아이엔씨. | 바이러스 벡터의 자동화된 생산 |
| IL284214B2 (en) | 2018-12-21 | 2025-03-01 | Octane Biotech Inc | Carousel for modular biologic production units |
| SG11202106652WA (en) | 2018-12-28 | 2021-07-29 | Octane Biotech Inc | Cell culture and tissue engineering systems with controlled environmental zones |
| IL285288B2 (en) | 2019-02-08 | 2026-04-01 | Lonza Walkersville Inc | Cell concentration methods and devices for use in automated bioreactors |
| CN114729292A (zh) | 2019-10-24 | 2022-07-08 | 奥克泰生物科技股份有限公司 | 具有经改进的细胞接触表面的细胞培养室 |
| EP4058563A4 (en) | 2019-11-11 | 2023-12-06 | Lonza Walkersville, Inc. | QUALITY CONTROL PROCEDURES FOR AUTOMATED CELL PROCESSING |
| US12187784B2 (en) | 2021-07-02 | 2025-01-07 | Combangio, Inc. | Processes for making a cellular fibronectin composition |
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| US3904480A (en) * | 1972-10-24 | 1975-09-09 | Lilly Co Eli | Enhanced production of plasminogen activator |
| JPS5329985A (en) * | 1976-08-27 | 1978-03-20 | Kanegafuchi Chem Ind Co Ltd | High concentration culturing of yeast |
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| US4232124A (en) * | 1979-04-23 | 1980-11-04 | Mann George F | Method of producing plasminogen activator |
| DE3015699C2 (de) * | 1979-04-26 | 1982-07-15 | Asahi Kasei Kogyo K.K., Osaka | Herstellung eines Plasminogen-Aktivators |
| JPS5642584A (en) * | 1979-09-18 | 1981-04-20 | Asahi Chem Ind Co Ltd | Cell cultivation method |
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| JPS5951220A (ja) * | 1982-08-02 | 1984-03-24 | Asahi Chem Ind Co Ltd | 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤 |
| US4537860A (en) * | 1982-12-08 | 1985-08-27 | Monsanto Company | Static cell culture maintenance system |
| NZ206699A (en) * | 1982-12-30 | 1989-08-29 | Bio Response Inc | Process for the production of serum independent cell lines |
| AU572108B2 (en) * | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
| JPS59121172U (ja) * | 1983-02-04 | 1984-08-15 | ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− | ケーブル電線被覆の剥離部分のカバー装置 |
| US4546083A (en) * | 1983-04-22 | 1985-10-08 | Stolle Research & Development Corporation | Method and device for cell culture growth |
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-
1987
- 1987-06-02 PT PT84991A patent/PT84991B/pt unknown
- 1987-06-02 IL IL8274687A patent/IL82746A/en not_active IP Right Cessation
- 1987-06-03 HU HU872536A patent/HU205171B/hu unknown
- 1987-06-03 NZ NZ220547A patent/NZ220547A/en unknown
- 1987-06-04 KR KR1019870005638A patent/KR930002888B1/ko not_active Expired - Lifetime
- 1987-06-04 DK DK198702881A patent/DK175366B1/da not_active IP Right Cessation
- 1987-06-05 AU AU73872/87A patent/AU601984B2/en not_active Expired
- 1987-06-05 ES ES198787304995T patent/ES2032444T3/es not_active Expired - Lifetime
- 1987-06-05 FR FR878707905A patent/FR2599625B1/fr not_active Expired - Lifetime
- 1987-06-05 DE DE8787304995T patent/DE3778758D1/de not_active Expired - Lifetime
- 1987-06-05 FI FI872526A patent/FI91886C/fi not_active IP Right Cessation
- 1987-06-05 NO NO872395A patent/NO170595C/no unknown
- 1987-06-05 DE DE3718939A patent/DE3718939C2/de not_active Expired - Lifetime
- 1987-06-05 EP EP87304995A patent/EP0248675B1/en not_active Expired - Lifetime
- 1987-06-05 JP JP62142121A patent/JPS6332484A/ja active Granted
- 1987-06-05 GB GB8713267A patent/GB2191493B/en not_active Expired - Lifetime
- 1987-06-05 AT AT87304995T patent/ATE75775T1/de not_active IP Right Cessation
- 1987-06-05 IE IE150487A patent/IE60485B1/en not_active IP Right Cessation
- 1987-06-05 DD DD30357587A patent/DD257087A5/de unknown
- 1987-06-05 ZA ZA874054A patent/ZA874054B/xx unknown
-
1990
- 1990-05-09 US US07/522,073 patent/US5053334A/en not_active Expired - Lifetime
-
1992
- 1992-07-21 GR GR920401579T patent/GR3005245T3/el unknown
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| MK9A | Patent expired |