IE60485B1 - Process for producing biological active plasminogen activator - Google Patents
Process for producing biological active plasminogen activatorInfo
- Publication number
- IE60485B1 IE60485B1 IE150487A IE150487A IE60485B1 IE 60485 B1 IE60485 B1 IE 60485B1 IE 150487 A IE150487 A IE 150487A IE 150487 A IE150487 A IE 150487A IE 60485 B1 IE60485 B1 IE 60485B1
- Authority
- IE
- Ireland
- Prior art keywords
- plasminogen activator
- medium
- type plasminogen
- tissue
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 33
- 102000001938 Plasminogen Activators Human genes 0.000 title abstract description 8
- 108010001014 Plasminogen Activators Proteins 0.000 title abstract description 8
- 229940127126 plasminogen activator Drugs 0.000 title abstract description 8
- 101000801481 Homo sapiens Tissue-type plasminogen activator Proteins 0.000 claims abstract description 31
- 230000001627 detrimental effect Effects 0.000 claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 13
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- 108090000373 Tissue Plasminogen Activator Proteins 0.000 claims description 19
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- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 claims description 3
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
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- -1 huaaan fc-ΡΑ Proteins 0.000 description 2
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
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- DSGKWFGEUBCEIE-UHFFFAOYSA-N (2-carbonochloridoylphenyl) acetate Chemical compound CC(=O)OC1=CC=CC=C1C(Cl)=O DSGKWFGEUBCEIE-UHFFFAOYSA-N 0.000 description 1
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- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
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- 241000763212 Lype Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000801924 Sena Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
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- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
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- 238000007865 diluting Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
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- 229940012952 fibrinogen Drugs 0.000 description 1
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- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
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- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 235000004252 protein component Nutrition 0.000 description 1
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- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Diabetes (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87164286A | 1986-06-06 | 1986-06-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE871504L IE871504L (en) | 1987-12-06 |
| IE60485B1 true IE60485B1 (en) | 1994-07-27 |
Family
ID=25357832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE150487A IE60485B1 (en) | 1986-06-06 | 1987-06-05 | Process for producing biological active plasminogen activator |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5053334A (enExample) |
| EP (1) | EP0248675B1 (enExample) |
| JP (1) | JPS6332484A (enExample) |
| KR (1) | KR930002888B1 (enExample) |
| AT (1) | ATE75775T1 (enExample) |
| AU (1) | AU601984B2 (enExample) |
| DD (1) | DD257087A5 (enExample) |
| DE (2) | DE3718939C2 (enExample) |
| DK (1) | DK175366B1 (enExample) |
| ES (1) | ES2032444T3 (enExample) |
| FI (1) | FI91886C (enExample) |
| FR (1) | FR2599625B1 (enExample) |
| GB (1) | GB2191493B (enExample) |
| GR (1) | GR3005245T3 (enExample) |
| HU (1) | HU205171B (enExample) |
| IE (1) | IE60485B1 (enExample) |
| IL (1) | IL82746A (enExample) |
| NO (1) | NO170595C (enExample) |
| NZ (1) | NZ220547A (enExample) |
| PT (1) | PT84991B (enExample) |
| ZA (1) | ZA874054B (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL82746A (en) * | 1986-06-06 | 1994-10-07 | Genentech Inc | Process for producing biologically active tissue-type plasminogen activator |
| JP2576200B2 (ja) * | 1988-03-09 | 1997-01-29 | 味の素株式会社 | 生理活性タンパク質の製造法 |
| EP0338716A3 (en) * | 1988-04-21 | 1990-04-11 | Berlex Laboratories, Inc. | Method and system for the production of non-degraded proteins from mammalian cells |
| DE3829244A1 (de) * | 1988-08-29 | 1990-03-01 | Boehringer Mannheim Gmbh | Verfahren zur herstellung von einkettigem t-pa |
| EP0391080A3 (de) * | 1989-03-07 | 1990-11-07 | Roche Diagnostics GmbH | Verfahren zur Herstellung von zweikettigem t-PA |
| GB2249099B (en) * | 1990-09-26 | 1995-05-03 | Squibb & Sons Inc | Squalene synthetase |
| US5677184A (en) * | 1994-04-19 | 1997-10-14 | Takeda Chemical Industries, Ltd. | CHO cells that express human LH-RH receptor |
| US6077940A (en) | 1997-12-24 | 2000-06-20 | Genentech, Inc. | Free solution ligand interaction molecular separation method |
| KR20050006147A (ko) | 2002-04-08 | 2005-01-15 | 밀레늄 바이올로직스 인코포레이티드 | 자동화된 조직 엔지니어링 시스템 |
| PT2634243T (pt) * | 2006-07-14 | 2017-12-18 | Patheon Holdings I B V | Método para melhorar a cultura de células |
| EP3327132A3 (en) * | 2007-08-09 | 2018-07-18 | Wyeth LLC | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| EP2525899A1 (en) * | 2010-01-22 | 2012-11-28 | Lonza Walkersville, Inc. | High yield method and apparatus for volume reduction and washing of therapeutic cells using tangential flow filtration |
| US10934519B2 (en) | 2011-07-29 | 2021-03-02 | Global Life Sciences Solutions Usa Llc | Systems, methods and control laws for cell harvesting |
| WO2014031690A1 (en) * | 2012-08-20 | 2014-02-27 | Terumo Bct, Inc. | Concentrating components of fluid circulated through a cell growth chamber |
| KR20250111214A (ko) | 2017-09-01 | 2025-07-22 | 론차 콜로그네 게엠베하 | 엔드-투-엔드 세포 요법의 자동화 |
| US12460174B2 (en) | 2018-09-28 | 2025-11-04 | Octane Biotech Inc. | Magnetic separation |
| CA3123314A1 (en) | 2018-12-21 | 2020-06-25 | Octane Biotech Inc. | Carousel for modular biologic production units |
| CA3123449A1 (en) | 2018-12-21 | 2020-06-25 | Lonza Walkersville, Inc. | Automated production of viral vectors |
| SG11202106652WA (en) | 2018-12-28 | 2021-07-29 | Octane Biotech Inc | Cell culture and tissue engineering systems with controlled environmental zones |
| CN113498432A (zh) | 2019-02-08 | 2021-10-12 | 隆萨沃克斯维尔股份有限公司 | 用于自动化生物反应器的细胞浓缩方法和装置 |
| IL292409A (en) | 2019-10-24 | 2022-06-01 | Octane Biotech Inc | A cell culture chamber with enhanced cell contact surfaces |
| KR20220097457A (ko) | 2019-11-11 | 2022-07-07 | 론자 워커스빌 아이엔씨. | 자동화된 세포 처리를 위한 품질 관리 방법 |
| JP2024525479A (ja) | 2021-07-02 | 2024-07-12 | コンバンジョ・インコーポレイテッド | 細胞性フィブロネクチン組成物を作製及び使用するためのプロセス |
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| US3904480A (en) * | 1972-10-24 | 1975-09-09 | Lilly Co Eli | Enhanced production of plasminogen activator |
| JPS5329985A (en) * | 1976-08-27 | 1978-03-20 | Kanegafuchi Chem Ind Co Ltd | High concentration culturing of yeast |
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| US4409331A (en) * | 1979-03-28 | 1983-10-11 | Damon Corporation | Preparation of substances with encapsulated cells |
| US4232124A (en) * | 1979-04-23 | 1980-11-04 | Mann George F | Method of producing plasminogen activator |
| DE3015699C2 (de) * | 1979-04-26 | 1982-07-15 | Asahi Kasei Kogyo K.K., Osaka | Herstellung eines Plasminogen-Aktivators |
| JPS5642584A (en) * | 1979-09-18 | 1981-04-20 | Asahi Chem Ind Co Ltd | Cell cultivation method |
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| IL68561A (en) * | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
| JPS5951220A (ja) * | 1982-08-02 | 1984-03-24 | Asahi Chem Ind Co Ltd | 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤 |
| US4537860A (en) * | 1982-12-08 | 1985-08-27 | Monsanto Company | Static cell culture maintenance system |
| NZ206699A (en) * | 1982-12-30 | 1989-08-29 | Bio Response Inc | Process for the production of serum independent cell lines |
| IE56716B1 (en) * | 1983-01-19 | 1991-11-20 | Genentech Inc | Method for producing human tpa and expression vectors therefor |
| JPS59121172U (ja) * | 1983-02-04 | 1984-08-15 | ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− | ケーブル電線被覆の剥離部分のカバー装置 |
| US4546083A (en) * | 1983-04-22 | 1985-10-08 | Stolle Research & Development Corporation | Method and device for cell culture growth |
| EP0143081B1 (en) * | 1983-11-21 | 1989-08-23 | Ciba-Geigy Ag | Synthesis of tissue plasminogen activator(tpa) by yeast |
| US4740461A (en) * | 1983-12-27 | 1988-04-26 | Genetics Institute, Inc. | Vectors and methods for transformation of eucaryotic cells |
| US4888115A (en) * | 1983-12-29 | 1989-12-19 | Cuno, Incorporated | Cross-flow filtration |
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| WO1986005514A1 (en) * | 1985-03-22 | 1986-09-25 | Chiron Corporation | EXPRESSION OF tPA IN MAMMALIAN CELLS |
| AU593264B2 (en) * | 1985-07-10 | 1990-02-08 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor |
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-
1987
- 1987-06-02 IL IL8274687A patent/IL82746A/en not_active IP Right Cessation
- 1987-06-02 PT PT84991A patent/PT84991B/pt unknown
- 1987-06-03 HU HU872536A patent/HU205171B/hu unknown
- 1987-06-03 NZ NZ220547A patent/NZ220547A/en unknown
- 1987-06-04 DK DK198702881A patent/DK175366B1/da not_active IP Right Cessation
- 1987-06-04 KR KR1019870005638A patent/KR930002888B1/ko not_active Expired - Lifetime
- 1987-06-05 FR FR878707905A patent/FR2599625B1/fr not_active Expired - Lifetime
- 1987-06-05 EP EP87304995A patent/EP0248675B1/en not_active Expired - Lifetime
- 1987-06-05 GB GB8713267A patent/GB2191493B/en not_active Revoked
- 1987-06-05 JP JP62142121A patent/JPS6332484A/ja active Granted
- 1987-06-05 ES ES198787304995T patent/ES2032444T3/es not_active Expired - Lifetime
- 1987-06-05 DE DE3718939A patent/DE3718939C2/de not_active Expired - Lifetime
- 1987-06-05 DD DD30357587A patent/DD257087A5/de unknown
- 1987-06-05 DE DE8787304995T patent/DE3778758D1/de not_active Expired - Lifetime
- 1987-06-05 AT AT87304995T patent/ATE75775T1/de not_active IP Right Cessation
- 1987-06-05 NO NO872395A patent/NO170595C/no unknown
- 1987-06-05 AU AU73872/87A patent/AU601984B2/en not_active Expired
- 1987-06-05 ZA ZA874054A patent/ZA874054B/xx unknown
- 1987-06-05 IE IE150487A patent/IE60485B1/en not_active IP Right Cessation
- 1987-06-05 FI FI872526A patent/FI91886C/fi not_active IP Right Cessation
-
1990
- 1990-05-09 US US07/522,073 patent/US5053334A/en not_active Expired - Lifetime
-
1992
- 1992-07-21 GR GR920401579T patent/GR3005245T3/el unknown
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| Date | Code | Title | Description |
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| MK9A | Patent expired |