DE3778758D1 - Verfahren zur herstellung von biologisch aktivem plasminogenaktivator. - Google Patents

Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.

Info

Publication number
DE3778758D1
DE3778758D1 DE8787304995T DE3778758T DE3778758D1 DE 3778758 D1 DE3778758 D1 DE 3778758D1 DE 8787304995 T DE8787304995 T DE 8787304995T DE 3778758 T DE3778758 T DE 3778758T DE 3778758 D1 DE3778758 D1 DE 3778758D1
Authority
DE
Germany
Prior art keywords
plasminogen activator
biologically active
medium
culture
producing biologically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8787304995T
Other languages
German (de)
English (en)
Inventor
William R Arathoon
Stuart E Builder
Anthony S Lubiniecki
Reis Robert D Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DE3778758D1 publication Critical patent/DE3778758D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE8787304995T 1986-06-06 1987-06-05 Verfahren zur herstellung von biologisch aktivem plasminogenaktivator. Expired - Lifetime DE3778758D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US87164286A 1986-06-06 1986-06-06

Publications (1)

Publication Number Publication Date
DE3778758D1 true DE3778758D1 (de) 1992-06-11

Family

ID=25357832

Family Applications (2)

Application Number Title Priority Date Filing Date
DE8787304995T Expired - Lifetime DE3778758D1 (de) 1986-06-06 1987-06-05 Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.
DE3718939A Expired - Lifetime DE3718939C2 (de) 1986-06-06 1987-06-05 Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3718939A Expired - Lifetime DE3718939C2 (de) 1986-06-06 1987-06-05 Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator

Country Status (21)

Country Link
US (1) US5053334A (enExample)
EP (1) EP0248675B1 (enExample)
JP (1) JPS6332484A (enExample)
KR (1) KR930002888B1 (enExample)
AT (1) ATE75775T1 (enExample)
AU (1) AU601984B2 (enExample)
DD (1) DD257087A5 (enExample)
DE (2) DE3778758D1 (enExample)
DK (1) DK175366B1 (enExample)
ES (1) ES2032444T3 (enExample)
FI (1) FI91886C (enExample)
FR (1) FR2599625B1 (enExample)
GB (1) GB2191493B (enExample)
GR (1) GR3005245T3 (enExample)
HU (1) HU205171B (enExample)
IE (1) IE60485B1 (enExample)
IL (1) IL82746A (enExample)
NO (1) NO170595C (enExample)
NZ (1) NZ220547A (enExample)
PT (1) PT84991B (enExample)
ZA (1) ZA874054B (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT84991B (pt) * 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo
JP2576200B2 (ja) * 1988-03-09 1997-01-29 味の素株式会社 生理活性タンパク質の製造法
EP0338716A3 (en) * 1988-04-21 1990-04-11 Berlex Laboratories, Inc. Method and system for the production of non-degraded proteins from mammalian cells
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
EP0391080A3 (de) * 1989-03-07 1990-11-07 Roche Diagnostics GmbH Verfahren zur Herstellung von zweikettigem t-PA
GB2249099B (en) * 1990-09-26 1995-05-03 Squibb & Sons Inc Squalene synthetase
US5677184A (en) * 1994-04-19 1997-10-14 Takeda Chemical Industries, Ltd. CHO cells that express human LH-RH receptor
US6077940A (en) 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
US8492140B2 (en) * 2002-04-08 2013-07-23 Octane Biotech Inc. Automated tissue engineering system
PT2634243T (pt) 2006-07-14 2017-12-18 Patheon Holdings I B V Método para melhorar a cultura de células
EP2188371B1 (en) * 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
WO2011091248A1 (en) * 2010-01-22 2011-07-28 Lonza Walkersville, Inc. High yield method and apparatus for volume reduction and washing of therapeutic cells using tangential flow filtration
US10934519B2 (en) 2011-07-29 2021-03-02 Global Life Sciences Solutions Usa Llc Systems, methods and control laws for cell harvesting
EP2885392A1 (en) * 2012-08-20 2015-06-24 Terumo BCT, Inc. Concentrating components of fluid circulated through a cell growth chamber
JP2020528284A (ja) 2017-09-01 2020-09-24 ロンザ ウォーカーズヴィル，インコーポレーテッド エンドツーエンド細胞療法の自動化
CN112752847B (zh) 2018-09-28 2025-03-28 奥克泰生物科技股份有限公司 磁性分离
CA3123314A1 (en) 2018-12-21 2020-06-25 Octane Biotech Inc. Carousel for modular biologic production units
EP3898994A4 (en) 2018-12-21 2022-12-14 Lonza Walkersville, Inc. AUTOMATED VIRAL VECTOR PRODUCTION
CN119685160A (zh) 2018-12-28 2025-03-25 奥克泰生物科技股份有限公司 具有受控环境区的细胞培养和组织工程系统
CN113498432A (zh) 2019-02-08 2021-10-12 隆萨沃克斯维尔股份有限公司 用于自动化生物反应器的细胞浓缩方法和装置
WO2021081170A1 (en) 2019-10-24 2021-04-29 Octane Biotech Inc. Cell culture chamber with improved cell-contacting surfaces
KR20220097457A (ko) 2019-11-11 2022-07-07 론자 워커스빌 아이엔씨. 자동화된 세포 처리를 위한 품질 관리 방법
CA3224150A1 (en) 2021-07-02 2023-01-05 Combangio, Inc. Processes for making and using a cellular fibronectin composition

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
JPS5329985A (en) * 1976-08-27 1978-03-20 Kanegafuchi Chem Ind Co Ltd High concentration culturing of yeast
JPS5571496A (en) * 1978-11-24 1980-05-29 Asahi Chem Ind Co Ltd Preparation of living substance
US4409331A (en) * 1979-03-28 1983-10-11 Damon Corporation Preparation of substances with encapsulated cells
US4232124A (en) * 1979-04-23 1980-11-04 Mann George F Method of producing plasminogen activator
DE3015699C2 (de) * 1979-04-26 1982-07-15 Asahi Kasei Kogyo K.K., Osaka Herstellung eines Plasminogen-Aktivators
JPS5642584A (en) * 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
US4420398A (en) * 1981-08-13 1983-12-13 American National Red Cross Filteration method for cell produced antiviral substances
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
US4537860A (en) * 1982-12-08 1985-08-27 Monsanto Company Static cell culture maintenance system
NZ206699A (en) * 1982-12-30 1989-08-29 Bio Response Inc Process for the production of serum independent cell lines
IE56716B1 (en) * 1983-01-19 1991-11-20 Genentech Inc Method for producing human tpa and expression vectors therefor
JPS59121172U (ja) * 1983-02-04 1984-08-15 ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− ケーブル電線被覆の剥離部分のカバー装置
US4546083A (en) * 1983-04-22 1985-10-08 Stolle Research & Development Corporation Method and device for cell culture growth
EP0143081B1 (en) * 1983-11-21 1989-08-23 Ciba-Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4888115A (en) * 1983-12-29 1989-12-19 Cuno, Incorporated Cross-flow filtration
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
JPH0740942B2 (ja) * 1985-03-22 1995-05-10 カイロン コ−ポレイシヨン 哺乳動物細胞におけるｔＰＡの発現
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
AU606582B2 (en) * 1985-09-06 1991-02-14 Codon Methods for the recovery of tissue plasminogen activator
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置
PT84991B (pt) * 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo

Also Published As

Publication number Publication date
EP0248675B1 (en) 1992-05-06
NO872395L (no) 1987-12-07
NO170595C (no) 1992-11-04
FR2599625A1 (fr) 1987-12-11
ZA874054B (en) 1989-02-22
IL82746A (en) 1994-10-07
NO872395D0 (no) 1987-06-05
DK175366B1 (da) 2004-09-13
DE3718939C2 (de) 1998-09-17
FI91886B (fi) 1994-05-13
FI872526L (fi) 1987-12-07
KR930002888B1 (ko) 1993-04-12
GR3005245T3 (enExample) 1993-05-24
NZ220547A (en) 1990-01-29
IL82746A0 (en) 1987-12-20
US5053334A (en) 1991-10-01
AU601984B2 (en) 1990-09-27
IE871504L (en) 1987-12-06
GB2191493B (en) 1991-02-27
JPS6332484A (ja) 1988-02-12
DK288187D0 (da) 1987-06-04
DD257087A5 (de) 1988-06-01
HU205171B (en) 1992-03-30
ES2032444T3 (es) 1993-02-16
DE3718939A1 (de) 1987-12-17
KR880000590A (ko) 1988-03-28
AU7387287A (en) 1987-12-10
NO170595B (no) 1992-07-27
HUT43875A (en) 1987-12-28
FI872526A0 (fi) 1987-06-05
JPH0574346B2 (enExample) 1993-10-18
GB8713267D0 (en) 1987-07-08
EP0248675A1 (en) 1987-12-09
GB2191493A (en) 1987-12-16
DK288187A (da) 1987-12-07
PT84991B (pt) 1990-03-08
FR2599625B1 (fr) 1992-03-13
FI91886C (fi) 1994-08-25
IE60485B1 (en) 1994-07-27
ATE75775T1 (de) 1992-05-15
PT84991A (en) 1987-07-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Free format text: BARZ, P., DIPL.-CHEM. DR.RER.NAT., PAT.-ANW., 80803 MUENCHEN