GB2519906B - Methods for detecting target nucleic acids in sample lysate droplets - Google Patents

Methods for detecting target nucleic acids in sample lysate droplets

Info

Publication number
GB2519906B
GB2519906B GB1503594.2A GB201503594A GB2519906B GB 2519906 B GB2519906 B GB 2519906B GB 201503594 A GB201503594 A GB 201503594A GB 2519906 B GB2519906 B GB 2519906B
Authority
GB
United Kingdom
Prior art keywords
methods
nucleic acids
target nucleic
detecting target
sample lysate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1503594.2A
Other languages
English (en)
Other versions
GB201503594D0 (en
GB2519906A (en
Inventor
R Abate Adam
Jay Eastburn Dennis
R Sciambi Adam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Priority to GB1616219.0A priority Critical patent/GB2539836B/en
Publication of GB201503594D0 publication Critical patent/GB201503594D0/en
Publication of GB2519906A publication Critical patent/GB2519906A/en
Application granted granted Critical
Publication of GB2519906B publication Critical patent/GB2519906B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4335—Mixers with a converging-diverging cross-section
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B13/00—Pumps specially modified to deliver fixed or variable measured quantities
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
GB1503594.2A 2012-08-13 2013-08-12 Methods for detecting target nucleic acids in sample lysate droplets Active GB2519906B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1616219.0A GB2539836B (en) 2012-08-13 2013-08-12 Methods for detecting target nucleic acids in sample lysate droplets

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261682707P 2012-08-13 2012-08-13
US201361784754P 2013-03-14 2013-03-14
PCT/US2013/054517 WO2014028378A2 (en) 2012-08-13 2013-08-12 Methods and systems for detecting biological components

Publications (3)

Publication Number Publication Date
GB201503594D0 GB201503594D0 (en) 2015-04-15
GB2519906A GB2519906A (en) 2015-05-06
GB2519906B true GB2519906B (en) 2017-02-08

Family

ID=50101583

Family Applications (2)

Application Number Title Priority Date Filing Date
GB1503594.2A Active GB2519906B (en) 2012-08-13 2013-08-12 Methods for detecting target nucleic acids in sample lysate droplets
GB1616219.0A Active GB2539836B (en) 2012-08-13 2013-08-12 Methods for detecting target nucleic acids in sample lysate droplets

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB1616219.0A Active GB2539836B (en) 2012-08-13 2013-08-12 Methods for detecting target nucleic acids in sample lysate droplets

Country Status (8)

Country Link
US (7) US10161007B2 (cg-RX-API-DMAC7.html)
EP (2) EP4001426A1 (cg-RX-API-DMAC7.html)
JP (1) JP6514105B2 (cg-RX-API-DMAC7.html)
CN (2) CN104736725A (cg-RX-API-DMAC7.html)
AU (3) AU2013302867A1 (cg-RX-API-DMAC7.html)
CA (1) CA2881783A1 (cg-RX-API-DMAC7.html)
GB (2) GB2519906B (cg-RX-API-DMAC7.html)
WO (1) WO2014028378A2 (cg-RX-API-DMAC7.html)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4001426A1 (en) 2012-08-13 2022-05-25 The Regents of The University of California Methods and systems for detecting biological components
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9731293B2 (en) * 2012-10-03 2017-08-15 The United States Of America, As Represented By The Secretary Of The Navy Paired laser and electrokinetic separation, manipulation, and analysis device
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
NL2012483B1 (en) * 2014-03-20 2016-01-18 Stichting Incas3 Method and system for mapping a three-dimensional structure using motes.
DE102014205531B4 (de) * 2014-03-25 2025-01-09 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zum Analysieren einer Probe biologischen Materials
CN106460066A (zh) * 2014-06-11 2017-02-22 赛普有限责任公司 通过液滴分选的核苷酸序列排除富集(needls)
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
US20170292153A1 (en) * 2014-10-14 2017-10-12 Bgi Shenzhen Co., Limited Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent
EP3206791B1 (fr) * 2014-10-17 2020-12-09 Ecole Polytechnique Procédé de manipulation de microgouttes incluant des échantillons
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
CN106103742B (zh) 2015-01-06 2019-12-10 深圳市海普洛斯生物科技有限公司 一种富集循环肿瘤dna的方法和试剂
MX367432B (es) 2015-01-12 2019-08-08 10X Genomics Inc Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos.
SG10201906653YA (en) * 2015-01-21 2019-09-27 Agency Science Tech & Res Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level
WO2016126865A1 (en) * 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
EP3253479B1 (en) 2015-02-04 2022-09-21 The Regents of The University of California Sequencing of nucleic acids via barcoding in discrete entities
US11634707B2 (en) * 2015-02-10 2023-04-25 Illumina, Inc. Methods and compositions for analyzing cellular components
CN105936930A (zh) * 2015-03-04 2016-09-14 松下知识产权经营株式会社 Dna检测方法和dna检测装置
CN106032551B (zh) * 2015-03-17 2020-07-21 东源生物医药科技（上海）有限公司 检测病毒样品中回复突变的方法和用于该方法的试剂盒
WO2016149661A1 (en) * 2015-03-18 2016-09-22 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
ES2954456T3 (es) * 2015-03-25 2023-11-22 Massachusetts Gen Hospital Análisis digital de células tumorales circulantes en muestras de sangre
WO2016197103A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
WO2016197106A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
US11123740B2 (en) 2015-06-29 2021-09-21 Arizona Board Of Regents On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
EP3361231B1 (en) * 2015-10-07 2021-02-17 AFI Corporation Inspection device, inspection system, and inspection method
AU2016335375A1 (en) * 2015-10-09 2018-04-26 Sysmex Corporation Analyte treatment chip, analyte treatment device, and analyte treatment method
CN208140648U (zh) * 2015-10-14 2018-11-23 阿尔卑斯电气株式会社 流路结构体以及测定对象液体的测定装置
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP2017118859A (ja) * 2015-12-31 2017-07-06 ローラス株式会社 滅菌型液体成分検査装置及び滅菌型液体成分検査装置の使用方法
CN105665049B (zh) * 2016-01-28 2017-07-04 清华大学深圳研究生院 一种疏液微阀式微量液体提取装置和提取方法
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
JP6765826B2 (ja) * 2016-03-10 2020-10-07 シスメックス株式会社 検体処理方法、検体処理チップおよび検体処理装置
JP6654951B2 (ja) 2016-03-31 2020-02-26 株式会社エンプラス 流体取扱装置
AU2017297416A1 (en) * 2016-07-12 2019-01-31 Northeastern University Single cell fluorescence in situ hybridization in microfluidic droplets
WO2018017794A1 (en) * 2016-07-21 2018-01-25 President And Fellows Of Harvard College Microfluidic sequencing techniques
CN110088290A (zh) * 2016-08-10 2019-08-02 加利福尼亚大学董事会 在乳液微滴中结合多重置换扩增和pcr
CN109964126B (zh) 2016-09-12 2022-12-27 伊索普莱克西斯公司 用于细胞治疗法和其他免疫治疗法的多重分析的系统和方法
CN106442443B (zh) * 2016-09-12 2018-12-07 北京天健惠康生物科技有限公司 一种微液滴荧光检测系统
EP3985127A1 (en) 2016-10-27 2022-04-20 The General Hospital Corporation Digital analysis of blood samples to determine efficacy of cancer therapies for specific cancers
EP3321352A1 (en) * 2016-11-09 2018-05-16 Biomillenia SAS Auxotrophic selection system
EP3538891B1 (en) * 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
EP3544737B1 (en) 2016-11-28 2025-04-23 Arizona Board of Regents on behalf of Arizona State University Methods related to continuous flow droplet reaction
CN108148744A (zh) * 2016-12-06 2018-06-12 中山百慧生物科技有限公司 一种液滴数字pcr芯片及相应检测方法和检测系统
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN106644900B (zh) * 2017-02-27 2023-10-03 大连海事大学 一种基于非均匀电场的阻抗脉冲颗粒计数装置及其计数方法
JP2018162990A (ja) * 2017-03-24 2018-10-18 株式会社エンプラス 液体取扱装置、液体取扱方法および液体取扱システム
US11213824B2 (en) 2017-03-29 2022-01-04 The Research Foundation For The State University Of New York Microfluidic device and methods
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
US11131673B2 (en) 2017-04-27 2021-09-28 Northeastern University Live single-cell bioassay in microdroplets
CN107219194B (zh) * 2017-05-03 2019-07-02 哈尔滨工业大学 具备应激响应的图案化微纳米结构的聚电解质复合物的制备方法及应用
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US20210208045A9 (en) * 2017-06-12 2021-07-08 Biomillenia Sas Method Of Measuring Viscosity In A Microfluidic System
EP3421131A1 (en) * 2017-06-30 2019-01-02 Blink AG A sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets
CN109201128A (zh) * 2017-07-05 2019-01-15 京东方科技集团股份有限公司 微流控芯片、化学发光免疫分析系统和分析方法
KR101897250B1 (ko) * 2017-07-11 2018-09-10 광주과학기술원 항암제 조합에 대한 단일 세포 분석 칩
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
WO2019022753A1 (en) * 2017-07-28 2019-01-31 Hewlett-Packard Development Company, L.P. INTERROGATION AND DETECTION OF IONIC SPECIES
WO2019036505A1 (en) 2017-08-14 2019-02-21 Mission Bio, Inc. METHODS AND COMPOSITIONS FOR EVALUATING THE UNIFORMITY OF AN EMULSION
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
ES2998086T3 (en) 2017-09-29 2025-02-19 Univ California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019079640A1 (en) * 2017-10-18 2019-04-25 Mission Bio, Inc. METHOD, SYSTEMS, AND DEVICE FOR HIGH-EFFICIENCY UNICELLULAR DNA SEQUENCING OF DROPLET MICROFLUIDS
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
CN109746062B (zh) * 2017-11-06 2024-04-12 北京新羿生物科技有限公司 微液滴生成装置
CN109746058B (zh) * 2017-11-06 2024-05-24 北京新羿生物科技有限公司 微液滴检测芯片
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US11707736B2 (en) * 2017-12-08 2023-07-25 Enuvio Inc. Microfluidic chip and method for making the same
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN107868752B (zh) * 2017-12-19 2024-01-05 广东顺德墨赛生物科技有限公司 夹具
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
CN110064451B (zh) * 2018-01-24 2023-08-15 思纳福(苏州)生命科技有限公司 流体驱动机构及流体驱动方法
US11052407B1 (en) * 2018-01-29 2021-07-06 The Texas A&M University System Dielectrophoretic in-droplet material concentrator
US20200360929A1 (en) * 2018-01-29 2020-11-19 The Texas A&M University System Integrated Modular On-Chip Droplet Microfluidic Screening Platform
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
JP2019170363A (ja) * 2018-03-29 2019-10-10 東ソー株式会社 標的分子の反応装置及び反応方法
SG11202009478PA (en) 2018-04-02 2020-10-29 Dropworks Inc Systems and methods for serial flow emulsion processes
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
CN112469504B (zh) 2018-05-23 2024-08-16 米罗库鲁斯公司 对数字微流控中的蒸发的控制
WO2019227126A1 (en) * 2018-05-28 2019-12-05 AI Fluidics Pty Ltd Method and appratus for controlling and manipulation of multi-phase flow in microfluidics using artificial intelligence
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN110579616B (zh) * 2018-06-07 2023-12-01 洛阳华清天木生物科技有限公司 一种微液滴处理装置及其使用方法
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
CN109145713B (zh) * 2018-07-02 2021-09-28 南京师范大学 一种结合目标检测的小目标语义分割方法
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
EP3837377A4 (en) 2018-08-17 2022-05-18 The Regents of University of California DROPLET FORMATION TRIGGERED BY MONODISPERSE PARTICLES FROM STEADY JETS
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
AU2019344001B2 (en) 2018-09-20 2023-11-16 Cepheid System, device and methods of sample processing using semiconductor detection chips
FI3856404T3 (fi) 2018-09-28 2024-10-29 Fluent Biosciences Inc Kohteen sieppaus monodispergoituihin pisaroihin
CN110193387A (zh) * 2018-10-16 2019-09-03 长春技特生物技术有限公司 一种全封闭微流控芯片和乳液微滴制备系统
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN109632437B (zh) * 2018-12-14 2020-07-24 北京化工大学 一种基于离子迁移谱的现场检测的样品前处理方法
AU2019398452A1 (en) * 2018-12-14 2021-07-08 Cepheid Diagnostic detection chip devices and methods of manufacture and assembly
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20220062899A1 (en) * 2019-01-18 2022-03-03 Mission Bio, Inc. Method, Apparatus and System to Detect Sub-Particle Flowrate in a Closed System
CA3126435A1 (en) * 2019-01-31 2020-08-06 Miroculus Inc. Non fouling compositions and methods for manipulating and processing encapsulated droplets
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CN114206499B (zh) 2019-04-08 2024-10-08 米罗库鲁斯公司 多盒式数字微流控装置和使用方法
WO2020223675A1 (en) * 2019-05-02 2020-11-05 The Regents Of The University Of California Laser-induced confocal microscope and microfluidic device for dielectrophoretic fluorescence-activated droplet sorting
AU2020275792B2 (en) 2019-05-14 2025-06-19 Scribe Biosciences, Inc. Platform for the deterministic assembly of microfluidic droplets
WO2020237222A1 (en) 2019-05-22 2020-11-26 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
AU2020325026A1 (en) 2019-08-05 2022-03-17 Mission Bio, Inc. Method and apparatus for single-cell analysis for determining a cell trajectory
WO2021030447A1 (en) * 2019-08-12 2021-02-18 Mission Bio, Inc. Method, system and apparatus for multi-omic simultaneous detection of protein expression, single nucleotide variations, and copy number variations in the same single cells
CN112439467B (zh) * 2019-08-29 2025-05-30 上海欣戈赛生物科技有限公司 用于制备乳化液滴的芯片及装置
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
GB201914266D0 (en) * 2019-10-03 2019-11-20 Univ Oxford Innovation Ltd Method of amplifying nucleic acids
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
WO2021112841A1 (en) 2019-12-04 2021-06-10 Hewlett-Packard Development Company, L.P. Sorting a droplet including a biologic sample
EP4073573A4 (en) 2019-12-10 2024-04-10 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
WO2021126969A1 (en) 2019-12-17 2021-06-24 The Regents Of The University Of California Selective and high-resolution printing of single cells
US20210215591A1 (en) * 2020-01-13 2021-07-15 Fluent Biosciences Inc. Devices for generating monodisperse droplets from a bulk liquid
LT6852B (lt) * 2020-01-27 2021-09-27 Innovation Fort Ltd Didelio lygiagretumo spartusis ląstelių skaitytuvas ir rūšiuoklis
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021168768A1 (zh) * 2020-02-28 2021-09-02 京东方科技集团股份有限公司 一种微流控芯片和微流控系统
CN111378562A (zh) * 2020-03-20 2020-07-07 中国科学院长春光学精密机械与物理研究所 数字pcr检测定量系统
CA3172238A1 (en) 2020-03-20 2021-09-23 Dalia Dhingra Single cell workflow for whole genome amplification
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP3925702A1 (en) * 2020-06-15 2021-12-22 Biomillenia SAS Method and system of producing a library of microorganisms
EP4153744A4 (en) 2020-06-26 2024-05-29 The Regents of University of California SELECTIVE ADDITION OF REAGENTS TO DROPLETS
WO2022038376A1 (en) * 2020-08-21 2022-02-24 Fluidic Analytics Limited H-type filter device for analysing a component
WO2022072363A1 (en) * 2020-09-29 2022-04-07 The Regents Of The University Of California Method for rapid and large-scale generation of droplets and droplet libraries
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2022138736A1 (ja) * 2020-12-25 2022-06-30 株式会社 TL Genomics シングルセルごとの特定の核酸のコピー数の検出方法
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN117241878A (zh) 2021-03-05 2023-12-15 伊努梅里斯公司 用于生成微滴和执行数字分析的系统和方法
US20220371018A1 (en) * 2021-05-18 2022-11-24 Canon Medical Systems Corporation Droplet collection unit, and droplet collection apparatus and method
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
CN114113314B (zh) * 2021-11-25 2024-03-22 厦门大学 一种用于滨海核电站的海洋致灾生物水声监测系统及方法
CN118922556A (zh) 2021-12-20 2024-11-08 伊努梅里斯公司 多个靶的检测和数字定量
US11857961B2 (en) 2022-01-12 2024-01-02 Miroculus Inc. Sequencing by synthesis using mechanical compression
KR102682799B1 (ko) * 2022-03-30 2024-07-09 광운대학교 산학협력단 3차원 전극 기반 미세유체 측정장치 및 그 제조방법
CN114921532A (zh) * 2022-04-29 2022-08-19 深圳大学 一种以非疾病诊断为目的的细胞定量及基因分型方法
EP4331724A1 (en) * 2022-08-29 2024-03-06 Institut Curie Method of extracting a bead from a droplet
EP4494762A1 (en) * 2023-07-21 2025-01-22 Blink AG Methods and devices for processing microparticles
CN117925391B (zh) * 2024-03-06 2025-12-30 东北林业大学 一种基于三内核双乳液滴可控融合的单细胞pcr装置及运行方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009054870A2 (en) * 2007-07-13 2009-04-30 Handylab, Inc. Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20110059556A1 (en) * 2009-09-04 2011-03-10 The Research Foundation Of State University Of New York Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices
WO2012083225A2 (en) * 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08503854A (ja) 1992-11-30 1996-04-30 ザ・ウエルカム・ファウンデーション・リミテッド 抗体のオリゴヌクレオチド複合体およびキレート化された放射性ヌクレオチドの相補的オリゴヌクレオチド複合体を用いる腫瘍部位の逐次ターゲッティング
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6319507B1 (en) 1997-05-02 2001-11-20 Kobo Products, Inc. Agar gel bead composition and method
EP1019496B1 (en) 1997-07-07 2004-09-29 Medical Research Council In vitro sorting method
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7041208B2 (en) 2001-12-11 2006-05-09 Sau Lan Tang Staats Microfluidic devices and methods for two-dimensional separations
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
CN102212614B (zh) 2003-01-29 2013-12-25 454生命科学公司 核酸扩增和测序的方法
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DE10339452A1 (de) 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
AU2003903295A0 (en) 2003-06-30 2003-07-10 Raustech Pty Ltd Substrate for combinatorial chemistry
EP2662136A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
CA2540087A1 (en) 2003-09-26 2005-04-07 Circleamp, Inc. Amplification of polynucleotides by rolling circle amplification
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
KR100647282B1 (ko) 2003-12-24 2006-11-17 삼성전자주식회사 ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법
US20060201868A1 (en) 2005-03-11 2006-09-14 Simmons Blake A Methods and devices for high-throughput dielectrophoretic concentration
US9285297B2 (en) 2005-08-22 2016-03-15 Applied Biosystems, Llc Device, system, and method for depositing processed immiscible-fluid-discrete-volumes
US8383059B2 (en) 2005-09-30 2013-02-26 University Of Utah Research Foundation Microfluidic interface for highly parallel addressing of sensing arrays
US9183349B2 (en) * 2005-12-16 2015-11-10 Nextbio Sequence-centric scientific information management
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US20080124726A1 (en) 2006-05-26 2008-05-29 Althea Technologies, Inc. Biochemical analysis of partitioned cells
WO2007141626A1 (en) 2006-06-02 2007-12-13 Pfizer Products Inc. Circulating tumor cell assay
PT2415873E (pt) * 2006-12-14 2015-03-31 Sangamo Biosciences Inc Proteínas com dedos de zinco não canónicas optimizadas
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB0720202D0 (en) 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
WO2009092106A1 (en) 2008-01-18 2009-07-23 The Regents Of The University Of California Accurate and rapid micromixer for integrated microfluidic devices
WO2009111014A2 (en) 2008-03-04 2009-09-11 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
WO2009137415A2 (en) * 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9194861B2 (en) * 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US20140179544A1 (en) 2012-09-07 2014-06-26 Bio-Rad Laboratories, Inc. Compositions, systems and methods for droplet formation, spacing and detection
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
AU2010266010B2 (en) 2009-06-26 2015-08-20 President And Fellows Of Harvard College Fluid injection
US8746285B2 (en) 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US9376713B2 (en) 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
AU2013203624B2 (en) 2010-02-09 2015-06-25 General Electric Company Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
ES2539047T3 (es) 2010-06-10 2015-06-25 Albert-Ludwigs-Universität Freiburg Aparato y método para dispensar células o partículas confinadas en una gota en vuelo libre
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
EP2595754B1 (en) 2010-07-22 2018-04-04 Gencell Biosystems Limited Composite liquid cells
US9695390B2 (en) 2010-08-23 2017-07-04 President And Fellows Of Harvard College Acoustic waves in microfluidics
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
EP3561159B1 (en) 2010-10-08 2023-09-20 President and Fellows of Harvard College High-throughput single cell barcoding
EP2643480A4 (en) 2010-11-22 2014-05-14 Solulink Inc METHOD AND / OR USE OF OLIGONUCLEOTIDE CONJUGATES FOR ASSAYS AND PROOF
WO2012102663A1 (en) 2011-01-24 2012-08-02 Ge Healthcare Bio-Sciences Ab Method of coupling binding agents to a substrate surface
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP2697397B1 (en) 2011-04-15 2017-04-05 The Johns Hopkins University Safe sequencing system
EP3789498B1 (en) 2011-04-25 2025-08-13 Bio-Rad Laboratories, Inc. Methods for nucleic acid analysis
WO2012162267A2 (en) 2011-05-20 2012-11-29 Fluidigm Corporation Nucleic acid encoding reactions
EP2714254B1 (en) 2011-05-23 2017-09-06 President and Fellows of Harvard College Control of emulsions, including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
GB2485635B (en) 2011-07-26 2012-11-28 Verinata Health Inc Method for determining the presence or absence of different aneuploidies in a sample
US20130032235A1 (en) 2011-08-02 2013-02-07 Teledyne Dalsa Semiconductor, Inc. Integrated microfluidic check valve and device including such a check valve
US9469874B2 (en) 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US9483374B2 (en) 2011-12-21 2016-11-01 Intel Corporation PSMI using at-speed scan capture
US9475013B2 (en) 2012-02-08 2016-10-25 President And Fellows Of Harvard College Droplet formation using fluid breakup
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
EP2850211B1 (en) 2012-05-14 2021-09-08 iRepertoire, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
EP4001426A1 (en) 2012-08-13 2022-05-25 The Regents of The University of California Methods and systems for detecting biological components
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
EP2898071A4 (en) 2012-09-21 2016-07-20 Broad Inst Inc COMPOSITIONS AND METHODS FOR LONG-TERM LABORATORIES AND PREPARED END OF NUCLEIC ACIDS IN EMULSION DROPS
AU2013350823B2 (en) 2012-11-27 2017-11-16 Gencell Biosystems Ltd. Handling liquid samples
EP2931919B1 (en) 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014108323A1 (en) 2013-01-10 2014-07-17 Eth Zurich Method and apparatus for depositing droplets onto a substrate
US20160011185A1 (en) 2013-03-04 2016-01-14 Gregory M. Lawrence Multi-analyte assay
US9752179B2 (en) 2013-03-14 2017-09-05 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014151658A1 (en) 2013-03-15 2014-09-25 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation
JP6545682B2 (ja) 2013-08-28 2019-07-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 大規模並列単一細胞分析
WO2015069798A1 (en) 2013-11-05 2015-05-14 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015179848A1 (en) 2014-05-23 2015-11-26 Firefly Bioworks, Inc. Substrate-mediated reactors for bioassays
CN106460066A (zh) 2014-06-11 2017-02-22 赛普有限责任公司 通过液滴分选的核苷酸序列排除富集(needls)
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
WO2016064755A2 (en) 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
EP3253479B1 (en) 2015-02-04 2022-09-21 The Regents of The University of California Sequencing of nucleic acids via barcoding in discrete entities
WO2016126865A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
WO2017031125A1 (en) 2015-08-17 2017-02-23 The Regents Of The University Of California Microdroplet-based multiple displacement amplification (mda) methods and related compositions
JP2019515674A (ja) 2016-04-19 2019-06-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法
CN110088290A (zh) 2016-08-10 2019-08-02 加利福尼亚大学董事会 在乳液微滴中结合多重置换扩增和pcr
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
CN110770352B (zh) 2017-11-17 2023-12-15 10X基因组学有限公司 用于关联生物颗粒的物理和遗传特性的方法和系统

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009054870A2 (en) * 2007-07-13 2009-04-30 Handylab, Inc. Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20110059556A1 (en) * 2009-09-04 2011-03-10 The Research Foundation Of State University Of New York Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices
WO2012083225A2 (en) * 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
American Association of Cancer Research (AACR), 2010, M Medkova et al, "Analyzing Cancer at Single Cell Resolution with Droplet Technology", *
Analytical Chemistry, 2010, Vol. 82, Z Yong et al, "High-performance single cell genetic analysis using microfluidic emulsion generator arrays", 3183 - 3190, *
Angew. Chem. Int. Ed., Vol 50, 2011, R Novak et al, "Single-Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose emulsions", 390-395 *
J Bioscience & Bioengineering, Vol 109, 2010, M Okochi et al, "Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system", 193-197 *

Also Published As

Publication number Publication date
US20160177375A1 (en) 2016-06-23
US10161007B2 (en) 2018-12-25
JP6514105B2 (ja) 2019-05-15
US20190169700A1 (en) 2019-06-06
US20190241965A1 (en) 2019-08-08
EP2882872B1 (en) 2021-10-06
CA2881783A1 (en) 2014-02-20
US20190330701A1 (en) 2019-10-31
US11203787B2 (en) 2021-12-21
CN110643681A (zh) 2020-01-03
GB201503594D0 (en) 2015-04-15
US20150232942A1 (en) 2015-08-20
EP4001426A1 (en) 2022-05-25
EP2882872A2 (en) 2015-06-17
AU2022203791B2 (en) 2025-02-13
GB201616219D0 (en) 2016-11-09
AU2022203791A1 (en) 2022-06-23
US20210388446A1 (en) 2021-12-16
WO2014028378A3 (en) 2014-04-17
CN104736725A (zh) 2015-06-24
US10745762B2 (en) 2020-08-18
AU2019226236B2 (en) 2022-03-03
AU2019226236A1 (en) 2019-09-26
AU2013302867A1 (en) 2015-02-26
WO2014028378A2 (en) 2014-02-20
GB2519906A (en) 2015-05-06
GB2539836A (en) 2016-12-28
US20240175091A1 (en) 2024-05-30
US11001896B2 (en) 2021-05-11
GB2539836B (en) 2017-03-29
EP2882872A4 (en) 2016-03-09
JP2015533079A (ja) 2015-11-19
US11891666B2 (en) 2024-02-06

Similar Documents

Publication Publication Date Title
GB2519906B (en) Methods for detecting target nucleic acids in sample lysate droplets
IL235128A0 (en) Preparation of a sample of nucleic acids
PT2850211T (pt) Método para aumentar a precisão na deteção quantitativa de polinucleótidos
ZA201406410B (en) Detection of nucleic acids
IL237480B (en) Methods for identifying genetic changes
EP2833144A4 (en) METHOD OF DETECTING BIOLOGICAL SUBSTANCE
SG11201400635UA (en) Method for detecting target nucleic acid
EP2971159A4 (en) Methods for analyzing nucleic acids
BR112014024421A2 (pt) métodos de preparação de amostras para amplificação de ácido nucleico
EP2840381A4 (en) METHOD FOR DETECTION OF TARGET PARTICLES
IL239258A0 (en) Multiplexed nucleic acid detection methods
EP2890783A4 (en) WITNESSES FOR NUCLEIC ACID ASSAYS
EP2978862A4 (en) DOUBLE-SINGLE ANTISOND COMPOSITIONS FOR DNA AND RNA DETECTION
EP2722388A4 (en) NUCLEIC ACID PROBE FOR THE ASSAY OF NUCLEIC ACIDS
EP3066218A4 (en) Methods for detecting nucleic acids
EP2740804A4 (en) PROCESS FOR NUCLEIC ACID ANALYSIS
EP2865764A4 (en) METHOD OF DETECTING NUCLEIC ACID AND DETECTION KIT THEREFOR
EP2829614A4 (en) METHOD FOR DETECTING A TARGET NUCLEIC ACID MOLECULE
EP2818850A4 (en) PROCEDURE FOR DETECTING TARGET PARTICLES
GB201302900D0 (en) Nucleic acid marker molecules for identifying and detecting cross contaminationof nucleic acid samples
PL2555872T3 (pl) Urządzenie do detekcji kwasów nukleinowych
IL238514A0 (en) Method and kit for detection of wild and/or mutated target DNA sequence
GB201308629D0 (en) Detecting low-abundant analyte in microdroplets
EP2872652A4 (en) DEVICES AND METHODS FOR PREPARING AND ANALYZING NUCLEIC ACIDS
EP3074537A4 (en) Methods for detecting nucleic acid proximity