BR112014024421A2 - métodos de preparação de amostras para amplificação de ácido nucleico - Google Patents

métodos de preparação de amostras para amplificação de ácido nucleico

Info

Publication number
BR112014024421A2
BR112014024421A2 BR112014024421A BR112014024421A BR112014024421A2 BR 112014024421 A2 BR112014024421 A2 BR 112014024421A2 BR 112014024421 A BR112014024421 A BR 112014024421A BR 112014024421 A BR112014024421 A BR 112014024421A BR 112014024421 A2 BR112014024421 A2 BR 112014024421A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
sample preparation
acid amplification
preparation methods
amplification
Prior art date
Application number
BR112014024421A
Other languages
English (en)
Inventor
Joseph Mcelgunn Cathal
pereira Clint
Carlo Tisi Laurence
Original Assignee
Lumora Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46160103&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112014024421(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lumora Ltd filed Critical Lumora Ltd
Publication of BR112014024421A2 publication Critical patent/BR112014024421A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/161—Temperature conditioning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus
    • B01D15/3876—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus modifying the temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
BR112014024421A 2012-03-30 2013-03-28 métodos de preparação de amostras para amplificação de ácido nucleico BR112014024421A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1205769.1A GB201205769D0 (en) 2012-03-30 2012-03-30 Methods for preparing samples for nucleic acid amplification
PCT/GB2013/050846 WO2013144654A1 (en) 2012-03-30 2013-03-28 Methods for preparing samples for nucleic acid amplification

Publications (1)

Publication Number Publication Date
BR112014024421A2 true BR112014024421A2 (pt) 2017-07-25

Family

ID=46160103

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014024421A BR112014024421A2 (pt) 2012-03-30 2013-03-28 métodos de preparação de amostras para amplificação de ácido nucleico

Country Status (12)

Country Link
US (1) US10246735B2 (pt)
EP (1) EP2831230B1 (pt)
JP (1) JP6325517B2 (pt)
CN (1) CN104487573B (pt)
AU (1) AU2013239408B2 (pt)
BR (1) BR112014024421A2 (pt)
CA (1) CA2868927A1 (pt)
ES (1) ES2622352T3 (pt)
GB (1) GB201205769D0 (pt)
IN (1) IN2014DN08573A (pt)
WO (1) WO2013144654A1 (pt)
ZA (1) ZA201407220B (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3059312A1 (en) * 2015-02-20 2016-08-24 QIAGEN GmbH Nucleic acid extraction method
GB201519565D0 (en) * 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
KR102498010B1 (ko) * 2016-09-28 2023-02-10 가부시키가이샤 후지미인코퍼레이티드 표면 처리 조성물
US11883831B2 (en) * 2017-04-06 2024-01-30 Shimadzu Corporation Magnetic particle operation device
KR102071058B1 (ko) * 2017-10-27 2020-03-02 주식회사 창 헬스케어 유전자 증폭을 위한 핵산의 추출 및 전달 자동화 장치 및 방법
CN107586774A (zh) * 2017-11-01 2018-01-16 上海涛济医药科技有限公司 一种常温下长时间口腔拭子dna保存和提取方法
BR112021000177A2 (pt) * 2018-07-11 2021-04-06 Monsanto Technology Llc Extração de polinucleotídeos
EP3663619B1 (en) * 2018-12-07 2022-03-16 Danfoss Power Solutions Aps Thermal relief device
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
JP2022058259A (ja) * 2020-09-30 2022-04-11 富佳生技股▲ふん▼有限公司 核酸検出キット及び核酸検出装置
CL2020002629A1 (es) * 2020-10-12 2021-01-15 Taag Genetics S A Nuevo dispositivo de biología molecular para extracción y purificación de ácidos nucleicos desde diferentes tipos de muestras biológicas que comprende resinas de adsorción
CN113637723B (zh) * 2021-07-09 2024-03-29 江苏康为世纪生物科技股份有限公司 一种粪便样本核酸保存液及其制备方法
EP4163369A1 (en) * 2021-10-08 2023-04-12 AXAGARIUS GmbH & Co. KG Use of mixtures of polyvinylpyrrolidone and ammonium sulfate in the isolation of nucleic acids
WO2023131534A1 (en) * 2022-01-10 2023-07-13 Certest Biotec, S.L. Method for processing nucleic-acid containing samples
CN117025379B (zh) * 2023-10-09 2023-12-15 迪飞医学科技(南京)有限公司 一种rapid恒温扩增核酸检测装置以及检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US555500A (en) 1896-03-03 Josef denk
US1994323A (en) * 1930-08-27 1935-03-12 Inez H Pelrce Coffee extracting device
JPH07509653A (ja) 1992-08-07 1995-10-26 アクゾ　ノーベル　ナムローゼ　フェンノートシャップ 混合物から残留物を除くための方法及び装置
GB2337261B (en) 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
WO2003033740A2 (en) * 2001-07-10 2003-04-24 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid
JP2006512102A (ja) 2002-04-11 2006-04-13 メディミューン・ヴァクシンズ・インコーポレーテッド 噴霧乾燥による生物活性材料の防腐
US20040126783A1 (en) * 2002-07-10 2004-07-01 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
CA2521999A1 (en) * 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
JP2005095003A (ja) 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
JP4399304B2 (ja) 2004-03-29 2010-01-13 富士フイルム株式会社 自動核酸分離精製方法およびその装置
JP2007306867A (ja) 2006-05-19 2007-11-29 Hitachi High-Technologies Corp 核酸抽出装置及び核酸抽出方法
US20080153096A1 (en) * 2006-11-02 2008-06-26 Vectrant Technologies Inc. Cartridge for conducting diagnostic assays
JP5011012B2 (ja) 2007-07-18 2012-08-29 株式会社日立ハイテクノロジーズ 核酸抽出装置
US9163228B2 (en) 2010-04-08 2015-10-20 Qiagen Gmbh Method for isolating and purifying nucleic acids

Also Published As

Publication number Publication date
CN104487573A (zh) 2015-04-01
EP2831230A1 (en) 2015-02-04
ES2622352T3 (es) 2017-07-06
WO2013144654A1 (en) 2013-10-03
AU2013239408B2 (en) 2018-03-08
JP6325517B2 (ja) 2018-05-16
US20150080562A1 (en) 2015-03-19
AU2013239408A1 (en) 2014-10-23
JP2015511497A (ja) 2015-04-20
GB201205769D0 (en) 2012-05-16
US10246735B2 (en) 2019-04-02
CN104487573B (zh) 2017-10-27
IN2014DN08573A (pt) 2015-05-15
EP2831230B1 (en) 2017-02-01
CA2868927A1 (en) 2013-10-03
ZA201407220B (en) 2017-01-25

Similar Documents

Publication Publication Date Title
BR112014025695A2 (pt) Preparação de amostra de ácido nucleico
BR112014024421A2 (pt) métodos de preparação de amostras para amplificação de ácido nucleico
BR112014024897A2 (pt) Método para amplificar ácidos nucleicos
IL245935A0 (en) Methods for RNA analysis
HK1217971A1 (zh) 用於核酸分析的方法、組合物和試劑盒
EP2718465A4 (en) PATTERNED RIVER CELLS FOR NUCLEIC ACID ANALYSIS
DK3623481T3 (da) Sammensætninger til nukleinsyresekventering
BR112014009066A2 (pt) dna triciclo-fosforotioato
DK2699698T4 (da) Oscillerende amplifikations-reaktion for nukleinsyrer
EP2702175A4 (en) METHOD AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS
GB2494239B (en) Nucleic acid amplification
BR112015023398A2 (pt) métodos para amplificar ácidos nucléicos
EP2764112A4 (en) DIRECT NUCLEIC ACID ANALYSIS
SG11201406717RA (en) Nucleic acid amplification
DK3152316T3 (da) Prøveklargøring til nukleinsyreamplifikation
GB201419367D0 (en) Nucleic acid probe
CL2015000943A1 (es) Muestreo embrionario para análisis molecular.
DK2890814T3 (da) Flerfaset nukleinsyreamplificering
EP2891709A4 (en) NUCLEIC ACID ANALYSIS DEVICE
EP2740804A4 (en) PROCESS FOR NUCLEIC ACID ANALYSIS
BR112014032072A2 (pt) método para detectar ácido nucleico e kit de detecção de ácido nucleico.
BR112016011692A2 (pt) amplificação de ácido nucléico
EP2872652A4 (en) DEVICES AND METHODS FOR PREPARING AND ANALYZING NUCLEIC ACIDS
EP2831260A4 (en) NUCLEIC ACIDS FOR NUCLEIC ACID AMPLIFICATION
DK3427723T3 (da) Rna-formulering til immunterapi

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time