FI70424B - Foerfarande foer framstaellning av rekombinant-dna som innehaoller en amylaskodande gen - Google Patents

Foerfarande foer framstaellning av rekombinant-dna som innehaoller en amylaskodande gen Download PDF

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Publication number
FI70424B
FI70424B FI810425A FI810425A FI70424B FI 70424 B FI70424 B FI 70424B FI 810425 A FI810425 A FI 810425A FI 810425 A FI810425 A FI 810425A FI 70424 B FI70424 B FI 70424B
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Finland
Prior art keywords
dna
lambda
amylase
phage
ncib
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FI810425A
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English (en)
Finnish (fi)
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FI810425L (fi
FI70424C (fi
Inventor
Charles Antoine Colson
Pierre Emile Cornelis
Colette Simone Digneffe
Raoul G P Walon
Corinne Walon
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Cpc International Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26274508&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI70424(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cpc International Inc filed Critical Cpc International Inc
Publication of FI810425L publication Critical patent/FI810425L/fi
Priority to FI821678A priority Critical patent/FI75367C/sv
Publication of FI70424B publication Critical patent/FI70424B/fi
Application granted granted Critical
Publication of FI70424C publication Critical patent/FI70424C/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (10)

1. Förfarande för framställning av rekombinant-DNA, som innehäller en amylaskodande gen, i vilket förfarande
5 DNA frän en amylasproducerande donormikroorganism av bakteriellt ursprung samt vektor-DNA spjälks och kombineras, de introduceras i en lämplig värd och de sälunda bildade klonerna sovras och väljs pä basis av närvaror av en amylaskodande gen, kanne-t e c k n a t därav, att vektorn är en plasmid eller DNA:n 10 av ett lambda-fagderivat.
2. Förfarande enligt patentkravet 1, kanne- t e c k n a t därav, att det omfattar ett ytterligare steg, i vilket den valda klonens DNA extraheras och spjälks, subklonas i en annan vektor och utväljs igen pä basis av 15 närvaror av en amylaskodande gen, varvid den andra vektorn är en plasmid eller DNA:n av ett annat lambda-fagderivat; och varvid denna subkloning eventuellt följs av en eller flera senare subkloningar.
3. Förfarande enligt patentkravet 1 eller 2, 20 kännetecknat därav, att valet utförs genom odling av klonerna i ett odlingsmedium innehällande stärkelse och genom färgning av mediet med jod genom att bringa det i kontakt med jodänga.
4. Förfarande enligt patentkravet 1 eller 2, 25 kännetecknat därav, att den amylaskodande genen är en pullulanaskodande gen och valet utförs genom odling av klonerna i ett BBL-tryptikasmedium innehällande pullulan, varvid positiva kloner identifieras medelst ogenomskinliga ringar, som omger dem. 30 5. Förfarande enligt patentkravet 1 eller 2, känne tecknat därav, att donormikroorganismen är en stam av släktet Bacillus.
6. Förfarande enligt patentkravet 1 eller 2, kännetecknat därav, att donormikroorganismen är Bacillus 35 megaterium, Bacillus coagulans, Bacillus cereus eller Klebsiella pneumoniae. il
FI810425A 1980-02-15 1981-02-12 Foerfarande foer framstaellning av rekombinant-dna som innehaoller en amylaskodande gen FI70424C (fi)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI821678A FI75367C (sv) 1980-02-15 1982-05-12 Förfarande för framställning av en genetiskt manipulerad mikroorganism innehållande en för amylas kodande rekombinant-DNA, och ett förfarand e för framställning av amylas

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB8005184 1980-02-15
GB8005184 1980-02-15
GB8011842 1980-04-10
GB8011842 1980-04-10

Publications (3)

Publication Number Publication Date
FI810425L FI810425L (fi) 1981-08-16
FI70424B true FI70424B (fi) 1986-03-27
FI70424C FI70424C (fi) 1986-09-19

Family

ID=26274508

Family Applications (1)

Application Number Title Priority Date Filing Date
FI810425A FI70424C (fi) 1980-02-15 1981-02-12 Foerfarande foer framstaellning av rekombinant-dna som innehaoller en amylaskodande gen

Country Status (16)

Country Link
US (1) US4469791A (sv)
EP (1) EP0034470B1 (sv)
CA (1) CA1183090A (sv)
DD (3) DD207553A5 (sv)
DE (1) DE3177286T2 (sv)
DK (1) DK162776B (sv)
ES (1) ES499391A0 (sv)
FI (1) FI70424C (sv)
GB (1) GB2069503B (sv)
GR (1) GR73678B (sv)
HU (1) HU195533B (sv)
IE (1) IE50919B1 (sv)
IL (1) IL61982A (sv)
KE (1) KE3310A (sv)
RU (1) RU1838410C (sv)
YU (2) YU43634B (sv)

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AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4493893A (en) * 1981-01-15 1985-01-15 Cpc International Inc. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis
US5525484A (en) * 1981-01-16 1996-06-11 Genome Therapeutics Corp. Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin
EP0076037B1 (en) * 1981-09-02 1988-12-07 Biogen, Inc. Amplified expression of dna sequences
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
ATE48156T1 (de) * 1982-11-01 1989-12-15 Miles Inc Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus.
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
JPS59196092A (ja) * 1983-04-25 1984-11-07 Sanraku Inc 組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
JP2669457B2 (ja) * 1983-07-06 1997-10-27 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 工業微生物種中の分子クローニングおよび発現
US4578352A (en) * 1983-07-13 1986-03-25 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
FR2556008B1 (fr) * 1983-12-06 1987-06-26 Centre Nat Rech Scient Vecteurs plasmidiques de clonage et d'expression d'une proteine dans un micro-organisme, comportant au moins le promoteur d'expression de la b-glucosidase dans les levures; micro-organismes les contenant; procede de fermentation et enzymes obtenues
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JPH062061B2 (ja) * 1984-06-14 1994-01-12 協和醗酵工業株式会社 Ｎ−アセチルノイラミン酸リア−ゼの製法
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
JPH0630586B2 (ja) * 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
JPS61162183A (ja) * 1985-01-07 1986-07-22 Agency Of Ind Science & Technol プルラナ−ゼ様酵素の製造法
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DE3688920D1 (de) * 1985-07-03 1993-09-30 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
SE8505027D0 (sv) * 1985-10-24 1985-10-24 Kabigen Ab A lytic virulent phage, a hostcell containing same and a process for protein production
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JPH01218589A (ja) * 1988-02-26 1989-08-31 Juzo Udaka 耐熱性β−アミラーゼ遺伝子
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US5583039A (en) * 1990-06-07 1996-12-10 Sunkyong Industries Ltd. Escherichia coli containing a gene encoding a maltogenic amylase BLMA of Bacillus licheniformis and gene product produced by same
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DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
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US5635468A (en) * 1993-05-19 1997-06-03 Kao Corporation Liquefying alkaline α-amylase, process for producing the same, and detergent composition containing the same
CA2173453C (en) * 1993-10-05 2001-02-13 Patricia A. Bower Cloned pullulanase
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
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JPS5811998B2 (ja) * 1975-12-19 1983-03-05 マルオ ブンジ シンキナアミラ−ゼノセイゾウホウホウ
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
IL58308A (en) * 1978-10-23 1983-10-31 Upjohn Co Process for preparing glucose isomerase using a recombinant plasmid

Also Published As

Publication number Publication date
GB2069503A (en) 1981-08-26
US4469791A (en) 1984-09-04
FI810425L (fi) 1981-08-16
EP0034470A2 (en) 1981-08-26
ES8202362A1 (es) 1982-01-01
YU44739B (en) 1991-02-28
DD158917A5 (de) 1983-02-09
HU195533B (en) 1988-05-30
IL61982A0 (en) 1981-02-27
IE810297L (en) 1981-08-15
DE3177286D1 (de) 1992-09-17
YU37781A (en) 1985-03-20
KE3310A (en) 1983-08-26
DK62881A (da) 1981-08-16
ES499391A0 (es) 1982-01-01
GR73678B (sv) 1984-03-30
DK162776B (da) 1991-12-09
RU1838410C (ru) 1993-08-30
CA1183090A (en) 1985-02-26
EP0034470B1 (en) 1992-08-12
EP0034470A3 (en) 1982-02-10
DD207221A5 (de) 1984-02-22
FI70424C (fi) 1986-09-19
YU86783A (en) 1985-10-31
YU43634B (en) 1989-10-31
DE3177286T2 (de) 1992-12-10
IE50919B1 (en) 1986-08-20
DD207553A5 (de) 1984-03-07
IL61982A (en) 1984-01-31
GB2069503B (en) 1983-04-20

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