FI75367C - Förfarande för framställning av en genetiskt manipulerad mikroorganism innehållande en för amylas kodande rekombinant-DNA, och ett förfarand e för framställning av amylas - Google Patents
Förfarande för framställning av en genetiskt manipulerad mikroorganism innehållande en för amylas kodande rekombinant-DNA, och ett förfarand e för framställning av amylas Download PDFInfo
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- FI75367C FI75367C FI821678A FI821678A FI75367C FI 75367 C FI75367 C FI 75367C FI 821678 A FI821678 A FI 821678A FI 821678 A FI821678 A FI 821678A FI 75367 C FI75367 C FI 75367C
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- Prior art keywords
- lambda
- amylase
- ncib
- dna
- coli
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Landscapes
- Enzymes And Modification Thereof (AREA)
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Claims (18)
1. Förfarande för framställning av en genetiskt mani-pulerad mikroorganism, varvid man i en värdmikroorganism 5 introducerar rekombinant-DNA, som innehiller en amylasko- dande gen och som framställts medelst ett in vitro-förfaran-de genom att klyva DNA, härlett frän en donormikroorganism, och genom att förena de bildade DNA-fragmenten med en pä samma sätt klyvd vektor, kännetecknat därav, 10 att vektorn är en plasmid eller DNA:n av ett derivat av en lambdafag och den genetiskt manipulerade mikroorganismen är en bakterie, som under lämpliga odlingsförhällanden förmär producera amylas i en väsentligt renare form eller i en väsentligt större mängd än den ursprungliga donormikroorga-15 nimsen producerar.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att donorbakterien är en stam av släk-tet Bacillus.
3. Förfarande enligt patentkravet 1, k ä n n e - 20 tecknat därav, att donorbakterien är Bacillus mega-terium, Bacillus coagulans, Bacillus cereus eller Klebsiella pneumoniae.
4. Förfarande enligt patentkravet 1 eller 3, kännetecknat därav, att donorbakterien är Bacillus 25 megaterium NCIB nr 11568, Bacillus coagulans NCIB nr 11571, Bacillus cereus ATCC nr 31102 eller Klebsiella pneumoniae ATCC nr 15050.
5. Förfarande enligt nägot av patentkraven 1-4, kännetecknat därav, att vektorn är plasmiden 30 pBR 322, pACYC 184 eller pC 194.
6. Förfarande enligt nägot av patentkraven 1-4, kännetecknat därav, att vektorn är fagen lambda NM 590, lambda NM 781 eller lambda NM 989.
7. Förfarande enligt nägot av patentkraven 1-4 eller 35 6,kännetecknat därav, att rekombinant-DNA:n omfattar fagerna lambda NM 590 Amy 1, NCIB nr 11569; lambda 36 7 5 3 6 7 NM 781 alfa Amy 1, NCIB nr 11572; lambda NM 781 beta Amy 1, NCIB nr 11574; lambda NM 781 Pul 1, NCIB nr 11593; och lambda NM 781 Pui 2, NCIB nr 11604.
8. Förfarande enligt nägot av patentkraven 1-5, 5 kännetecknat därav, att den genetiskt manipu-lerade mikroorganismen är E.coli eller B. subtilis.
9. Förfarande enligt nägot av patentkraven 1-5, kännetecknat därav, att den genetiskt manipu-lerade mikroorganismen är E. coli Cl 7001 (pCP 1), NCIB nr 10 11570; E. coli CL 7002 (pCP 2), NCIB nr 11573; E. coli CL 7003 (lambda alfa Amy 1), NCIB nr 11586; E. coli CL 7004 (pCP 3), NCIB nr 11602; E. coli CL 7005 (lambda beta Amy 1), nr 11603; E. coli CL 7006 (pCP 4), NCIB nr 11605; E. coli CL 7007 (lambda Pui 2), NCIB nr 11606; eller B. subtilis 15 CL 8001 (pCH 1), NCIB nr 11629.
10. Förfarande för framställning av amylas, kännetecknat därav, att en genetiskt manipulerad mikroorganism, framställd enligt nägot av patentkraven 1-9, odlas under amylasproducerande förhällanden. 20
11. Förfarande enligt patentkravet 10, känne tecknat därav, att rekombinant-DNA:n är närvarande i form av en lytisk fag och odlingen genomförs i en infek-terad bakterievärd.
12. Förfarande enligt patentkravet 10, k ä n n e -25 tecknat därav, att rekombinant-DNA:n är närvarande i form av en fag i lysogen E. coli och odlingen utförs sä, att mängfaldigandet av fagen induceras genom en värmebehand-ling.
13. Förfarande enligt patentkravet 10, k ä n n e -30 tecknat därav, att rekombinant-DNA:n är närvarande i den genetiskt manipulerade mikroorganismen i form av en plasmid och odlingen utförs tili stationär fas.
14. Förfarande enligt patentkravet 13, kännetecknat därav, att plasmiden är ett derivat av pBR 35 322 eller pACYC 184 och att mängfaldigandet förstärks genom odling av mikroorganismen i närvaro av kloramfenikol innan odlingen av denna tili stationär fas.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8005184 | 1980-02-15 | ||
| GB8005184 | 1980-02-15 | ||
| GB8011842 | 1980-04-10 | ||
| GB8011842 | 1980-04-10 | ||
| FI810425A FI70424C (fi) | 1980-02-15 | 1981-02-12 | Foerfarande foer framstaellning av rekombinant-dna som innehaoller en amylaskodande gen |
| FI810425 | 1981-02-12 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI821678L FI821678L (fi) | 1982-05-12 |
| FI821678A0 FI821678A0 (fi) | 1982-05-12 |
| FI75367B FI75367B (fi) | 1988-02-29 |
| FI75367C true FI75367C (sv) | 1990-11-04 |
Family
ID=27241044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI821678A FI75367C (sv) | 1980-02-15 | 1982-05-12 | Förfarande för framställning av en genetiskt manipulerad mikroorganism innehållande en för amylas kodande rekombinant-DNA, och ett förfarand e för framställning av amylas |
Country Status (1)
| Country | Link |
|---|---|
| FI (1) | FI75367C (sv) |
-
1982
- 1982-05-12 FI FI821678A patent/FI75367C/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI75367B (fi) | 1988-02-29 |
| FI821678L (fi) | 1982-05-12 |
| FI821678A0 (fi) | 1982-05-12 |
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| Date | Code | Title | Description |
|---|---|---|---|
| MM | Patent lapsed | ||
| MM | Patent lapsed |
Owner name: CPC INTERNATIONAL INC. |