DE3177286T2 - Genetisch modifizierte mikroorganismen fuer die massenherstellung amylolytischer enzyme und verfahren zu deren herstellung. - Google Patents

Genetisch modifizierte mikroorganismen fuer die massenherstellung amylolytischer enzyme und verfahren zu deren herstellung.

Info

Publication number
DE3177286T2
DE3177286T2 DE8181300578T DE3177286T DE3177286T2 DE 3177286 T2 DE3177286 T2 DE 3177286T2 DE 8181300578 T DE8181300578 T DE 8181300578T DE 3177286 T DE3177286 T DE 3177286T DE 3177286 T2 DE3177286 T2 DE 3177286T2
Authority
DE
Germany
Prior art keywords
production
enzyms
amylolytic
genetically modified
modified microorganisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8181300578T
Other languages
English (en)
Other versions
DE3177286D1 (de
Inventor
Charles Antoine Colson
Pierre Emile Cornelis
Colette Simone Digneffe
Raoul G P Walon
Corrine Walon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Application granted granted Critical
Publication of DE3177286D1 publication Critical patent/DE3177286D1/de
Publication of DE3177286T2 publication Critical patent/DE3177286T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE8181300578T 1980-02-15 1981-02-12 Genetisch modifizierte mikroorganismen fuer die massenherstellung amylolytischer enzyme und verfahren zu deren herstellung. Expired - Fee Related DE3177286T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8005184 1980-02-15
GB8011842 1980-04-10

Publications (2)

Publication Number Publication Date
DE3177286D1 DE3177286D1 (de) 1992-09-17
DE3177286T2 true DE3177286T2 (de) 1992-12-10

Family

ID=26274508

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8181300578T Expired - Fee Related DE3177286T2 (de) 1980-02-15 1981-02-12 Genetisch modifizierte mikroorganismen fuer die massenherstellung amylolytischer enzyme und verfahren zu deren herstellung.

Country Status (16)

Country Link
US (1) US4469791A (de)
EP (1) EP0034470B1 (de)
CA (1) CA1183090A (de)
DD (3) DD158917A5 (de)
DE (1) DE3177286T2 (de)
DK (1) DK162776B (de)
ES (1) ES499391A0 (de)
FI (1) FI70424C (de)
GB (1) GB2069503B (de)
GR (1) GR73678B (de)
HU (1) HU195533B (de)
IE (1) IE50919B1 (de)
IL (1) IL61982A (de)
KE (1) KE3310A (de)
RU (1) RU1838410C (de)
YU (2) YU43634B (de)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
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AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
ZA82133B (en) * 1981-01-15 1983-02-23 Cpc International Inc A process for cloning the gene coding for a thermostabic alpha amylase into escherichia coli and bacillus subtilis
US4493893A (en) * 1981-01-15 1985-01-15 Cpc International Inc. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis
US5525484A (en) * 1981-01-16 1996-06-11 Genome Therapeutics Corp. Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin
EP0076037B1 (de) * 1981-09-02 1988-12-07 Biogen, Inc. Verstärkte Expression von DNS-Sequenzen
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
EP0108301B2 (de) * 1982-11-01 1993-09-01 SOLVAY ENZYMES, INC. (a Delaware corporation) Verfahren zur heterologen Klonierung eines Gens in einem Bacillus-Mikroorganismus
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
JPS59196092A (ja) * 1983-04-25 1984-11-07 Sanraku Inc 組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
GR82200B (de) * 1983-07-06 1984-12-13 Gist Brocades Nv
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
US4578352A (en) * 1983-07-13 1986-03-25 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
FR2556008B1 (fr) * 1983-12-06 1987-06-26 Centre Nat Rech Scient Vecteurs plasmidiques de clonage et d'expression d'une proteine dans un micro-organisme, comportant au moins le promoteur d'expression de la b-glucosidase dans les levures; micro-organismes les contenant; procede de fermentation et enzymes obtenues
GB8333797D0 (en) * 1983-12-19 1984-01-25 Searle & Co Starch utilization
PH23920A (en) * 1983-12-20 1990-01-23 Cetus Corp Glucoamylase cdna
GB8414272D0 (en) * 1984-06-05 1984-07-11 Cpc International Inc Enzymatic hydrolysis
GB8414271D0 (en) * 1984-06-05 1984-07-11 Cpc International Inc Recombinant dna
JPH062061B2 (ja) * 1984-06-14 1994-01-12 協和醗酵工業株式会社 Ｎ−アセチルノイラミン酸リア−ゼの製法
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
JPH0630586B2 (ja) * 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
JPS61162183A (ja) * 1985-01-07 1986-07-22 Agency Of Ind Science & Technol プルラナ−ゼ様酵素の製造法
EP0221923A4 (de) * 1985-03-29 1990-03-22 Bioteknika International Sekretionsvektor.
ATE93541T1 (de) * 1985-07-03 1993-09-15 Genencor Int Hybride polypeptide und verfahren zu deren herstellung.
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
SE8505027D0 (sv) * 1985-10-24 1985-10-24 Kabigen Ab A lytic virulent phage, a hostcell containing same and a process for protein production
US5017477A (en) * 1985-10-25 1991-05-21 Biotechnica International, Inc. Enhancing DNA sequencies derived from the sacQ gene
FR2598431B1 (fr) * 1986-05-09 1990-02-09 Transgene Sa Souches de saccharomyces produisant de l'alpha-amylase
US4962026A (en) * 1986-09-15 1990-10-09 Enzyme Bio-Systems, Ltd. Process for the production of panosyl derivatives
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
JPH01218589A (ja) * 1988-02-26 1989-08-31 Juzo Udaka 耐熱性β−アミラーゼ遺伝子
US5171673A (en) * 1988-07-18 1992-12-15 Biotechnica International, Inc. Expression of heterologous dna using the bacillus coagulans amylase gene
US5209938A (en) * 1989-09-13 1993-05-11 Cpc International Inc. Method for retarding staling of baked goods
US5583039A (en) * 1990-06-07 1996-12-10 Sunkyong Industries Ltd. Escherichia coli containing a gene encoding a maltogenic amylase BLMA of Bacillus licheniformis and gene product produced by same
EP0541676A1 (de) * 1990-08-01 1993-05-19 Novo Nordisk A/S Hitzebeständige pullulanasen
DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
DK0670367T3 (da) * 1993-05-19 2003-11-24 Kao Corp Flydendegørende alkalisk alfa-amylase, fremgangsmåde til fremstilling af denne og detergentsammensætning indeholdende denne
CA2173453C (en) * 1993-10-05 2001-02-13 Patricia A. Bower Cloned pullulanase
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
US7220542B2 (en) * 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi

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Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
JPS5811998B2 (ja) * 1975-12-19 1983-03-05 マルオ ブンジ シンキナアミラ−ゼノセイゾウホウホウ
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
IL58308A (en) * 1978-10-23 1983-10-31 Upjohn Co Process for preparing glucose isomerase using a recombinant plasmid

Also Published As

Publication number Publication date
EP0034470A3 (en) 1982-02-10
YU43634B (en) 1989-10-31
FI70424C (fi) 1986-09-19
IE810297L (en) 1981-08-15
IE50919B1 (en) 1986-08-20
CA1183090A (en) 1985-02-26
ES8202362A1 (es) 1982-01-01
US4469791A (en) 1984-09-04
FI70424B (fi) 1986-03-27
YU44739B (en) 1991-02-28
DD207553A5 (de) 1984-03-07
DK162776B (da) 1991-12-09
YU86783A (en) 1985-10-31
DD207221A5 (de) 1984-02-22
IL61982A0 (en) 1981-02-27
IL61982A (en) 1984-01-31
HU195533B (en) 1988-05-30
DK62881A (da) 1981-08-16
GB2069503B (en) 1983-04-20
RU1838410C (ru) 1993-08-30
ES499391A0 (es) 1982-01-01
DE3177286D1 (de) 1992-09-17
FI810425L (fi) 1981-08-16
GR73678B (de) 1984-03-30
GB2069503A (en) 1981-08-26
KE3310A (en) 1983-08-26
YU37781A (en) 1985-03-20
EP0034470B1 (de) 1992-08-12
DD158917A5 (de) 1983-02-09
EP0034470A2 (de) 1981-08-26

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