DE3586145T2 - Rekombinante dns, diese enthaltende mikroorganismen und deren verwendung zur herstellung von amylasen. - Google Patents
Rekombinante dns, diese enthaltende mikroorganismen und deren verwendung zur herstellung von amylasen.Info
- Publication number
- DE3586145T2 DE3586145T2 DE8585303947T DE3586145T DE3586145T2 DE 3586145 T2 DE3586145 T2 DE 3586145T2 DE 8585303947 T DE8585303947 T DE 8585303947T DE 3586145 T DE3586145 T DE 3586145T DE 3586145 T2 DE3586145 T2 DE 3586145T2
- Authority
- DE
- Germany
- Prior art keywords
- recombinant dna
- amylase
- microorganism
- derived
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 244000005700 microbiome Species 0.000 title abstract 5
- 108020004511 Recombinant DNA Proteins 0.000 title abstract 4
- 102000013142 Amylases Human genes 0.000 title 1
- 108010065511 Amylases Proteins 0.000 title 1
- 229940025131 amylases Drugs 0.000 title 1
- 239000013612 plasmid Substances 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 241000194107 Bacillus megaterium Species 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 102000003832 Nucleotidyltransferases Human genes 0.000 abstract 1
- 108090000119 Nucleotidyltransferases Proteins 0.000 abstract 1
- 108090000637 alpha-Amylases Proteins 0.000 abstract 1
- 102000004139 alpha-Amylases Human genes 0.000 abstract 1
- 229940024171 alpha-amylase Drugs 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 229960000318 kanamycin Drugs 0.000 abstract 1
- 229930027917 kanamycin Natural products 0.000 abstract 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 abstract 1
- 229930182823 kanamycin A Natural products 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
- Y10S435/839—Bacillus subtilis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB848414271A GB8414271D0 (en) | 1984-06-05 | 1984-06-05 | Recombinant dna |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3586145D1 DE3586145D1 (de) | 1992-07-09 |
| DE3586145T2 true DE3586145T2 (de) | 1992-12-03 |
Family
ID=10561932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8585303947T Expired - Fee Related DE3586145T2 (de) | 1984-06-05 | 1985-06-04 | Rekombinante dns, diese enthaltende mikroorganismen und deren verwendung zur herstellung von amylasen. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US4806426A (de) |
| EP (1) | EP0165002B1 (de) |
| JP (1) | JPH0779683B2 (de) |
| AT (1) | ATE76902T1 (de) |
| AU (1) | AU4313285A (de) |
| BR (1) | BR8502681A (de) |
| CA (1) | CA1279590C (de) |
| DE (1) | DE3586145T2 (de) |
| DK (1) | DK170735B1 (de) |
| ES (2) | ES8702947A1 (de) |
| FI (1) | FI91885C (de) |
| GB (1) | GB8414271D0 (de) |
| IE (1) | IE58259B1 (de) |
| MX (1) | MX7725E (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4962026A (en) * | 1986-09-15 | 1990-10-09 | Enzyme Bio-Systems, Ltd. | Process for the production of panosyl derivatives |
| US5209938A (en) * | 1989-09-13 | 1993-05-11 | Cpc International Inc. | Method for retarding staling of baked goods |
| US6300115B1 (en) | 1998-05-18 | 2001-10-09 | Enzyme Bio-Systems Ltd. | Pullulanase expression constructs containing α-amylase promoter and leader sequences |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2759053A1 (de) * | 1977-12-30 | 1979-07-12 | Uhlin | Verfahren zum herstellen von genprodukten von plasmid-dns |
| ZA81424B (en) * | 1980-02-15 | 1982-02-24 | Cpc International Inc | Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
| IT1156317B (it) * | 1982-09-08 | 1987-02-04 | Anic Spa | Vettori plasmidici ad espressione in bacillus subtilis e procedimento per la loro preparazione |
| DK135983D0 (da) * | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
-
1984
- 1984-06-05 GB GB848414271A patent/GB8414271D0/en active Pending
-
1985
- 1985-05-22 IE IE128785A patent/IE58259B1/en not_active IP Right Cessation
- 1985-05-23 US US06/737,311 patent/US4806426A/en not_active Expired - Lifetime
- 1985-05-24 FI FI852084A patent/FI91885C/fi not_active IP Right Cessation
- 1985-05-30 AU AU43132/85A patent/AU4313285A/en not_active Abandoned
- 1985-06-03 MX MX85956U patent/MX7725E/es unknown
- 1985-06-04 DE DE8585303947T patent/DE3586145T2/de not_active Expired - Fee Related
- 1985-06-04 ES ES543860A patent/ES8702947A1/es not_active Expired
- 1985-06-04 DK DK251485A patent/DK170735B1/da not_active IP Right Cessation
- 1985-06-04 BR BR8502681A patent/BR8502681A/pt not_active IP Right Cessation
- 1985-06-04 EP EP85303947A patent/EP0165002B1/de not_active Expired - Lifetime
- 1985-06-04 AT AT85303947T patent/ATE76902T1/de not_active IP Right Cessation
- 1985-06-05 JP JP60120735A patent/JPH0779683B2/ja not_active Expired - Lifetime
- 1985-06-05 CA CA000483212A patent/CA1279590C/en not_active Expired - Fee Related
-
1986
- 1986-01-28 ES ES551307A patent/ES8802536A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0165002A2 (de) | 1985-12-18 |
| DK251485A (da) | 1985-12-06 |
| FI91885C (fi) | 1994-08-25 |
| DK251485D0 (da) | 1985-06-04 |
| AU4313285A (en) | 1985-12-12 |
| CA1279590C (en) | 1991-01-29 |
| ES8702947A1 (es) | 1987-01-16 |
| MX7725E (es) | 1991-01-30 |
| ES551307A0 (es) | 1988-07-16 |
| JPH0779683B2 (ja) | 1995-08-30 |
| ES8802536A1 (es) | 1988-07-16 |
| FI852084A0 (fi) | 1985-05-24 |
| DE3586145D1 (de) | 1992-07-09 |
| EP0165002A3 (en) | 1987-09-02 |
| GB8414271D0 (en) | 1984-07-11 |
| FI91885B (fi) | 1994-05-13 |
| ATE76902T1 (de) | 1992-06-15 |
| ES543860A0 (es) | 1987-01-16 |
| JPS6119491A (ja) | 1986-01-28 |
| EP0165002B1 (de) | 1992-06-03 |
| IE851287L (en) | 1985-12-05 |
| US4806426A (en) | 1989-02-21 |
| IE58259B1 (en) | 1993-08-25 |
| DK170735B1 (da) | 1995-12-27 |
| FI852084L (fi) | 1985-12-06 |
| BR8502681A (pt) | 1986-02-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |