ES8802536A1 - Un procedimiento para producir adn recombinante que contiene un gen que codifica amilasa (como divisional de la solicitud de patente de invencion numero 543.860 presentada el 4 de junio de 1985) - Google Patents

Un procedimiento para producir adn recombinante que contiene un gen que codifica amilasa (como divisional de la solicitud de patente de invencion numero 543.860 presentada el 4 de junio de 1985)

Info

Publication number
ES8802536A1
ES8802536A1 ES551307A ES551307A ES8802536A1 ES 8802536 A1 ES8802536 A1 ES 8802536A1 ES 551307 A ES551307 A ES 551307A ES 551307 A ES551307 A ES 551307A ES 8802536 A1 ES8802536 A1 ES 8802536A1
Authority
ES
Spain
Prior art keywords
recombinant dna
amylases
production
containing same
microorganisms containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551307A
Other languages
English (en)
Other versions
ES551307A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Publication of ES551307A0 publication Critical patent/ES551307A0/es
Publication of ES8802536A1 publication Critical patent/ES8802536A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL INVENTO COMPRENDE LA PRODUCCION DE ADN RECOMBINANTE QUE CONTIENE UN GEN QUE CODIFICA LA AMILASA, PREPARADO POR UN PROCEDIMIENTO IN VITRO. EL PROCEDIMIENTO CONSISTE EN ESCINDIR EL ADN PROCEDENTE DE UN MICROORGANISMO CLORANTE Y DEL PLASMIDO PUBLLO MEDIANTE EL CONTACTO CON UNA ENDONUCLEASA DE RESTRICCION, Y COMBINAR LOS FRAGMENTOS DE ADN RESULTANTES QUE CONTIENEN EL GEN QUE CODIFICA LA AMILASA CON UN VECTOR ESCINDIDO DEL PLASMIDO. LOS MICROORGANISMOS DONANTES SON: BACILLUS MEGATERIUM, BACILLUS COAGULANS, BACILLUS CIRCULANS, BACILLUS CEREUS Y KLEBSIELLA PNEUMONIAE.
ES551307A 1984-06-05 1986-01-28 Un procedimiento para producir adn recombinante que contiene un gen que codifica amilasa (como divisional de la solicitud de patente de invencion numero 543.860 presentada el 4 de junio de 1985) Expired ES8802536A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB848414271A GB8414271D0 (en) 1984-06-05 1984-06-05 Recombinant dna

Publications (2)

Publication Number Publication Date
ES551307A0 ES551307A0 (es) 1988-07-16
ES8802536A1 true ES8802536A1 (es) 1988-07-16

Family

ID=10561932

Family Applications (2)

Application Number Title Priority Date Filing Date
ES543860A Expired ES8702947A1 (es) 1984-06-05 1985-06-04 Un procedimiento para modificar la constitucion genetica de un microorganismo a fin de producir amidasa.
ES551307A Expired ES8802536A1 (es) 1984-06-05 1986-01-28 Un procedimiento para producir adn recombinante que contiene un gen que codifica amilasa (como divisional de la solicitud de patente de invencion numero 543.860 presentada el 4 de junio de 1985)

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES543860A Expired ES8702947A1 (es) 1984-06-05 1985-06-04 Un procedimiento para modificar la constitucion genetica de un microorganismo a fin de producir amidasa.

Country Status (14)

Country Link
US (1) US4806426A (es)
EP (1) EP0165002B1 (es)
JP (1) JPH0779683B2 (es)
AT (1) ATE76902T1 (es)
AU (1) AU4313285A (es)
BR (1) BR8502681A (es)
CA (1) CA1279590C (es)
DE (1) DE3586145T2 (es)
DK (1) DK170735B1 (es)
ES (2) ES8702947A1 (es)
FI (1) FI91885C (es)
GB (1) GB8414271D0 (es)
IE (1) IE58259B1 (es)
MX (1) MX7725E (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962026A (en) * 1986-09-15 1990-10-09 Enzyme Bio-Systems, Ltd. Process for the production of panosyl derivatives
US5209938A (en) * 1989-09-13 1993-05-11 Cpc International Inc. Method for retarding staling of baked goods
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2759053A1 (de) * 1977-12-30 1979-07-12 Uhlin Verfahren zum herstellen von genprodukten von plasmid-dns
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
IT1156317B (it) * 1982-09-08 1987-02-04 Anic Spa Vettori plasmidici ad espressione in bacillus subtilis e procedimento per la loro preparazione
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse

Also Published As

Publication number Publication date
BR8502681A (pt) 1986-02-12
DE3586145D1 (de) 1992-07-09
EP0165002B1 (en) 1992-06-03
JPS6119491A (ja) 1986-01-28
DK251485A (da) 1985-12-06
ES551307A0 (es) 1988-07-16
GB8414271D0 (en) 1984-07-11
MX7725E (es) 1991-01-30
CA1279590C (en) 1991-01-29
IE58259B1 (en) 1993-08-25
ES543860A0 (es) 1987-01-16
ES8702947A1 (es) 1987-01-16
US4806426A (en) 1989-02-21
DE3586145T2 (de) 1992-12-03
EP0165002A3 (en) 1987-09-02
FI91885C (fi) 1994-08-25
FI852084A0 (fi) 1985-05-24
DK251485D0 (da) 1985-06-04
IE851287L (en) 1985-12-05
FI852084L (fi) 1985-12-06
ATE76902T1 (de) 1992-06-15
EP0165002A2 (en) 1985-12-18
FI91885B (fi) 1994-05-13
AU4313285A (en) 1985-12-12
JPH0779683B2 (ja) 1995-08-30
DK170735B1 (da) 1995-12-27

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970701