ATE88216T1 - Erhoehung der expression von genen in mikroorganismen. - Google Patents

Erhoehung der expression von genen in mikroorganismen.

Info

Publication number
ATE88216T1
ATE88216T1 AT86308356T AT86308356T ATE88216T1 AT E88216 T1 ATE88216 T1 AT E88216T1 AT 86308356 T AT86308356 T AT 86308356T AT 86308356 T AT86308356 T AT 86308356T AT E88216 T1 ATE88216 T1 AT E88216T1
Authority
AT
Austria
Prior art keywords
enhancing
microorganisms
genes
expression
increase
Prior art date
Application number
AT86308356T
Other languages
English (en)
Inventor
Alan Sloma
Rosalind C Lee
Janice Pero
Original Assignee
Omnigene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Omnigene Inc filed Critical Omnigene Inc
Application granted granted Critical
Publication of ATE88216T1 publication Critical patent/ATE88216T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT86308356T 1985-10-25 1986-10-27 Erhoehung der expression von genen in mikroorganismen. ATE88216T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79135085A 1985-10-25 1985-10-25
US06/921,343 US5017477A (en) 1985-10-25 1986-10-20 Enhancing DNA sequencies derived from the sacQ gene
EP86308356A EP0227260B1 (de) 1985-10-25 1986-10-27 Erhöhung der Expression von Genen in Mikroorganismen

Publications (1)

Publication Number Publication Date
ATE88216T1 true ATE88216T1 (de) 1993-04-15

Family

ID=27121148

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86308356T ATE88216T1 (de) 1985-10-25 1986-10-27 Erhoehung der expression von genen in mikroorganismen.

Country Status (6)

Country Link
US (1) US5017477A (de)
EP (1) EP0227260B1 (de)
AT (1) ATE88216T1 (de)
CA (1) CA1313152C (de)
DE (1) DE3688282T2 (de)
DK (1) DK509886A (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2602793B1 (fr) * 1986-08-18 1989-04-07 Pasteur Institut Sequence d'adn exercant une fonction se manifestant par une surproduction de proteines exocellulaires par diverses souches de bacillus, et vecteurs contenant cette sequence
DE3711883A1 (de) * 1987-04-08 1988-10-27 Merck Patent Gmbh Genetische kontrolleinheit und klonierungs- und expressionssystem
FR2621597B1 (fr) * 1987-07-24 1992-07-31 Pasteur Institut Procede de clonage et d'expression de genes codant pour des proteines constituant des edifices fonctionnels organises
FR2618453A1 (fr) * 1987-07-24 1989-01-27 Pasteur Institut Procede de clonage et d'expression de genes codant pour des proteines constituant des edifices fonctionnels organises
DE3824827A1 (de) * 1988-07-21 1990-02-01 Henkel Kgaa Plasmide zur erzeugung von alpha-amylase in bacillus
WO1992003561A1 (en) * 1990-08-13 1992-03-05 Alko Ltd. HYBRID α-AMYLASE PROMOTERS
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
CN1080307C (zh) * 1993-02-26 2002-03-06 芬兰国家公众健康机构 促进革兰氏阳性细菌中产生商业重要的外蛋白的方法和系统
EP2000477A1 (de) * 2007-06-07 2008-12-10 DSM IP Assets B.V. Verbesserte Herstellung eines Zielprodukts durch mRNA-Stabilisierung
CN101679490B (zh) * 2007-06-07 2014-05-07 帝斯曼知识产权资产管理有限公司 通过mRNA的稳定化提高目标产物的生产
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
MX2023015455A (es) 2021-06-24 2024-02-23 Basf Se Huesped de produccion de bacillus mejorado.

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
WO1983000702A1 (en) * 1981-08-21 1983-03-03 University Patents Inc Enhancement of expression of protein synthesis in e. coli
EP0074553A3 (de) * 1981-09-11 1985-01-09 The University Of Rochester Verfahren zum Steigern der Ausbeute eines Produktes durch Modifizierung eines Mikroorganismus
ATE48156T1 (de) * 1982-11-01 1989-12-15 Miles Inc Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus.
US4554250A (en) * 1983-06-30 1985-11-19 E. R. Squibb & Sons, Inc. Method of increased production of penicillin acylase and plasmid employed therein
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
EP0146743B1 (de) * 1983-11-08 1992-07-29 Teijin Limited Genfragmente, abgeleitet vom menschlichen Immunoglobulin-Gen
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
JPH0789930B2 (ja) * 1984-03-08 1995-10-04 大正製薬株式会社 ウイルス性転写促進配列
CA1275954C (en) * 1984-08-31 1990-11-06 Hing Cheug Wong 3'-expression enhancing fragments and method
GB8521753D0 (en) * 1985-09-02 1985-10-09 Green M Oxide bronze materials

Also Published As

Publication number Publication date
US5017477A (en) 1991-05-21
DE3688282T2 (de) 1993-11-18
DK509886D0 (da) 1986-10-24
EP0227260B1 (de) 1993-04-14
CA1313152C (en) 1993-01-26
EP0227260A1 (de) 1987-07-01
DE3688282D1 (de) 1993-05-19
DK509886A (da) 1987-04-26

Similar Documents

Publication Publication Date Title
ATE88216T1 (de) Erhoehung der expression von genen in mikroorganismen.
ATE137265T1 (de) Bacillus-stämme
ATE242803T1 (de) Enzym mit proteaseaktivität
IL68366A (en) Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
HK99293A (en) Bacillus incapable of excreting subtilisin or neutral protease
NO172646C (no) Dna-sekvens, repliserbart ekspresjonsplasmid, samt fremstilling av insulin og human-insulin-forloepere
ATE65798T1 (de) Menschlicher granuloxcyt-koloniestimulierungsfaktor.
IL79291A (en) Gram-positive expression control sequences of the bacillus subtilis bacteria
MY102992A (en) Dna frangments containing alkaline cellulase gene, recombinant plasmids with said dna fragments inserted therein, and recombinant microorganisms.
AU682140B2 (en) Multidrug resistance gene
BR8205954A (pt) Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
DE3686399D1 (de) Herstellung von thrombin-inhibitoren.
BG45047A3 (bg) Метод за получаване на изопеницилин н синтетазна активност в гостоприемникова клетка
AU621810B2 (en) Amylolytic enzymes producing microorganisms, constructed by recombinant DNA technology and their use for fermentation processes
AU1682092A (en) Production of plants resistant to attacks of sclerotinia sclerotiorum by introducing a gene coding for an oxalate oxidase
EP0147178A3 (en) Expression plasmids for improved production of heterologous protein in bacteria
ATE111516T1 (de) Invasive mikroorganismen.
ATE275195T1 (de) Saccharose-stoffwechsel-mutanten
DE3683784D1 (de) Zugeordnete ribosomen und ihre verwendung zur herstellung von proteinen aus zellkulturen.
ATE129287T1 (de) Bakterielles kollagenase-gen.
ES8702947A1 (es) Un procedimiento para modificar la constitucion genetica de un microorganismo a fin de producir amidasa.
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
DE3685517D1 (de) Klonierung und expression von delta-endotoxin-genen von bacillus thuringiensis in bakterienzellen und daraus gewonnene produkte.
DE58908358D1 (de) Herstellung und Verwendung einer DNA zur Erhöhung der Ausbeute bei der Expression von rekombinanten Genen.
UA6228A1 (uk) Штамм hirschioporus laricinus m-81(karst)ryv -продуцент молокозвертаючого ферменту

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties