ES2270467T3 - Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada. - Google Patents

Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada. Download PDF

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Publication number
ES2270467T3
ES2270467T3 ES97934169T ES97934169T ES2270467T3 ES 2270467 T3 ES2270467 T3 ES 2270467T3 ES 97934169 T ES97934169 T ES 97934169T ES 97934169 T ES97934169 T ES 97934169T ES 2270467 T3 ES2270467 T3 ES 2270467T3
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Prior art keywords
probe
oligonucleotide
target
hybridization
oligonucleotides
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Expired - Lifetime
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ES97934169T
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English (en)
Spanish (es)
Inventor
Michael Mcclellan Becker
Mehrdad Majlessi
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Gen Probe Inc
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Gen Probe Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ES97934169T 1996-07-16 1997-07-15 Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada. Expired - Lifetime ES2270467T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2181896P 1996-07-16 1996-07-16
US21818P 1996-07-16

Publications (1)

Publication Number Publication Date
ES2270467T3 true ES2270467T3 (es) 2007-04-01

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ES97934169T Expired - Lifetime ES2270467T3 (es) 1996-07-16 1997-07-15 Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada.

Country Status (11)

Country Link
US (3) US6130038A (2)
EP (1) EP0912767B1 (2)
JP (2) JP2000514307A (2)
KR (1) KR20000023814A (2)
AT (1) ATE339514T1 (2)
AU (1) AU726821B2 (2)
CA (1) CA2260749A1 (2)
DE (1) DE69736667T2 (2)
DK (1) DK0912767T3 (2)
ES (1) ES2270467T3 (2)
WO (1) WO1998002582A2 (2)

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AU726821B2 (en) 2000-11-23
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US6130038A (en) 2000-10-10
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US6903206B1 (en) 2005-06-07
DK0912767T3 (da) 2006-10-30
ATE339514T1 (de) 2006-10-15
DE69736667T2 (de) 2007-09-06
WO1998002582A3 (en) 1998-07-02
EP0912767B1 (en) 2006-09-13
JP2000514307A (ja) 2000-10-31
AU3728897A (en) 1998-02-09
KR20000023814A (ko) 2000-04-25
WO1998002582A2 (en) 1998-01-22
US20050106610A1 (en) 2005-05-19
JP4527789B2 (ja) 2010-08-18
CA2260749A1 (en) 1998-01-22

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