ES2240753T3 - Metodo de deteccion de acido nucleico. - Google Patents
Metodo de deteccion de acido nucleico.Info
- Publication number
- ES2240753T3 ES2240753T3 ES02738330T ES02738330T ES2240753T3 ES 2240753 T3 ES2240753 T3 ES 2240753T3 ES 02738330 T ES02738330 T ES 02738330T ES 02738330 T ES02738330 T ES 02738330T ES 2240753 T3 ES2240753 T3 ES 2240753T3
- Authority
- ES
- Spain
- Prior art keywords
- probe
- multiplication
- sequence
- brand
- acceptor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 110
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 44
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 27
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title description 14
- 239000000523 sample Substances 0.000 claims abstract description 211
- 108020004414 DNA Proteins 0.000 claims abstract description 51
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 34
- 230000000295 complement effect Effects 0.000 claims abstract description 26
- 239000011230 binding agent Substances 0.000 claims abstract description 23
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 18
- 125000005647 linker group Chemical group 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims description 66
- 230000004568 DNA-binding Effects 0.000 claims description 24
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 13
- 238000009396 hybridization Methods 0.000 claims description 13
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 239000000975 dye Substances 0.000 claims description 10
- 239000000155 melt Substances 0.000 claims description 7
- 108091033319 polynucleotide Proteins 0.000 claims description 7
- 102000040430 polynucleotide Human genes 0.000 claims description 7
- 239000002157 polynucleotide Substances 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- BDJDTKYGKHEMFF-UHFFFAOYSA-M QSY7 succinimidyl ester Chemical compound [Cl-].C=1C=C2C(C=3C(=CC=CC=3)S(=O)(=O)N3CCC(CC3)C(=O)ON3C(CCC3=O)=O)=C3C=C\C(=[N+](\C)C=4C=CC=CC=4)C=C3OC2=CC=1N(C)C1=CC=CC=C1 BDJDTKYGKHEMFF-UHFFFAOYSA-M 0.000 claims description 3
- 239000007767 bonding agent Substances 0.000 claims description 3
- 230000001687 destabilization Effects 0.000 claims description 3
- 238000009830 intercalation Methods 0.000 claims description 3
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 claims description 3
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 claims description 2
- 238000013461 design Methods 0.000 claims description 2
- 239000000138 intercalating agent Substances 0.000 claims description 2
- 239000001022 rhodamine dye Substances 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- BCVRWZODRSBEQZ-UHFFFAOYSA-N LDS 751(1+) Chemical compound C1=CC2=CC(N(C)C)=CC=C2[N+](CC)=C1C=CC=CC1=CC=C(N(C)C)C=C1 BCVRWZODRSBEQZ-UHFFFAOYSA-N 0.000 claims 1
- 230000002687 intercalation Effects 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000000370 acceptor Substances 0.000 description 54
- 239000000047 product Substances 0.000 description 50
- 239000013615 primer Substances 0.000 description 37
- 108090000623 proteins and genes Proteins 0.000 description 22
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 17
- 108091093088 Amplicon Proteins 0.000 description 13
- 230000004044 response Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 238000006460 hydrolysis reaction Methods 0.000 description 9
- 102000007469 Actins Human genes 0.000 description 8
- 108010085238 Actins Proteins 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 230000007062 hydrolysis Effects 0.000 description 8
- 238000011002 quantification Methods 0.000 description 8
- 201000009906 Meningitis Diseases 0.000 description 7
- 230000001419 dependent effect Effects 0.000 description 7
- 239000002987 primer (paints) Substances 0.000 description 7
- 238000002844 melting Methods 0.000 description 6
- 230000008018 melting Effects 0.000 description 6
- 241000606161 Chlamydia Species 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- IIRDTKBZINWQAW-UHFFFAOYSA-N hexaethylene glycol Chemical compound OCCOCCOCCOCCOCCOCCO IIRDTKBZINWQAW-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000005382 thermal cycling Methods 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 108700024394 Exon Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 108010076504 Protein Sorting Signals Proteins 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- FGBAVQUHSKYMTC-UHFFFAOYSA-M LDS 751 dye Chemical compound [O-]Cl(=O)(=O)=O.C1=CC2=CC(N(C)C)=CC=C2[N+](CC)=C1C=CC=CC1=CC=C(N(C)C)C=C1 FGBAVQUHSKYMTC-UHFFFAOYSA-M 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 101150093941 PORA gene Proteins 0.000 description 2
- 241000239226 Scorpiones Species 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- -1 for example Chemical compound 0.000 description 2
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 230000004001 molecular interaction Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000606153 Chlamydia trachomatis Species 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 101150111720 EPSPS gene Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 235000000421 Lepidium meyenii Nutrition 0.000 description 1
- 240000000759 Lepidium meyenii Species 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
- 241000588650 Neisseria meningitidis Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229940038705 chlamydia trachomatis Drugs 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 235000012902 lepidium meyenii Nutrition 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 238000002165 resonance energy transfer Methods 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Geophysics And Detection Of Objects (AREA)
- Eye Examination Apparatus (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0112868.5A GB0112868D0 (en) | 2001-05-25 | 2001-05-25 | Detection system |
| GB0112868 | 2001-05-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2240753T3 true ES2240753T3 (es) | 2005-10-16 |
Family
ID=9915364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02738330T Expired - Lifetime ES2240753T3 (es) | 2001-05-25 | 2002-05-24 | Metodo de deteccion de acido nucleico. |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7700275B2 (enExample) |
| EP (1) | EP1390542B1 (enExample) |
| JP (1) | JP2004532044A (enExample) |
| KR (1) | KR20040024556A (enExample) |
| CN (1) | CN1304596C (enExample) |
| AT (1) | ATE298804T1 (enExample) |
| AU (1) | AU2002311414B2 (enExample) |
| CA (1) | CA2448211C (enExample) |
| DE (1) | DE60204874T2 (enExample) |
| DK (1) | DK1390542T3 (enExample) |
| ES (1) | ES2240753T3 (enExample) |
| GB (1) | GB0112868D0 (enExample) |
| PT (1) | PT1390542E (enExample) |
| WO (1) | WO2002097132A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| GB0223563D0 (en) * | 2002-10-10 | 2002-11-20 | Secr Defence | Detection system |
| ES2529204T3 (es) * | 2004-10-18 | 2015-02-17 | Brandeis University | Métodos para la amplificación de ácido nucleico |
| WO2006121277A1 (en) * | 2005-05-09 | 2006-11-16 | Korea Research Institute Of Standards And Science | Fret probes for fluorescent detection of the epsps gene |
| KR100764559B1 (ko) * | 2005-05-09 | 2007-10-08 | 한국표준과학연구원 | Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브 |
| CN100420943C (zh) * | 2005-06-23 | 2008-09-24 | 李卫 | Dna分子内杂交-聚丙烯酰胺凝胶电泳方法 |
| US8968700B2 (en) * | 2005-08-11 | 2015-03-03 | The Board Of Trustees Of The Leland Stanford Junior University | Imaging of protease activity in live cells using activity based probes |
| US7785786B2 (en) | 2006-01-23 | 2010-08-31 | Quest Diagnostics Investments Incorporated | Methods for detecting nucleic acids using multiple signals |
| CN100526461C (zh) * | 2006-07-10 | 2009-08-12 | 中国农业科学院棉花研究所 | 获得植物内含子序列扩增多态性的方法及其专用引物 |
| JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
| US8658366B2 (en) | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| US20100129796A1 (en) * | 2008-11-24 | 2010-05-27 | Micah Halpern | Dye probe fluorescence resonance energy transfer genotyping |
| GB201004339D0 (en) | 2010-03-16 | 2010-04-28 | Enigma Diagnostics Ltd | Sequence detection assay |
| GB201007868D0 (en) | 2010-05-11 | 2010-06-23 | Enigma Diagnostics Ltd | Sequence detection assay |
| WO2012118715A2 (en) | 2011-02-28 | 2012-09-07 | The Board Of Trustees Of Leland Stanford Junior University | Non-peptidic quenched fluorescent imaging probes |
| DE102011117520A1 (de) * | 2011-11-03 | 2013-05-08 | Frank Schleifenbaum | Sensorsystem für nicht intrusive Druckmessungen |
| WO2014145257A2 (en) | 2013-03-15 | 2014-09-18 | The Board Of Trustees Of The Leland Stanford Junior University | Activity-based probe compounds, compositions, and methods of use |
| EP3347714B1 (en) * | 2015-09-10 | 2021-03-10 | InSilixa Inc. | Methods for multiplex quantitative nucleic acid amplification |
| EP3510380B1 (en) | 2016-12-23 | 2023-11-08 | The Board of Trustees of the Leland Stanford Junior University | Activity-based probe compounds, compositions, and methods of use |
| EP3601261A4 (en) | 2017-03-30 | 2021-07-14 | The Board of Trustees of the Leland Stanford Junior University | CONTRAST AGENTS ACTIVATED BY A PROTEASE FOR IN VIVO IMAGING |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1533151A (fr) * | 1962-05-18 | 1968-07-19 | Rhone Poulenc Sa | Nouvel antibiotique et sa préparation |
| US4012284A (en) * | 1962-11-16 | 1977-03-15 | Societa' Farmaceutici Italia, S.p.A. | Process of preparation of antibiotic F.I. 1762 derivatives |
| US3183157A (en) * | 1963-02-01 | 1965-05-11 | Upjohn Co | Antibiotic nogalamycin and method of producing |
| US4197249A (en) * | 1977-08-15 | 1980-04-08 | American Cyanamid Company | 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| CA1219824A (en) | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| HU201626B (en) | 1989-09-05 | 1990-11-28 | Magyar Kabel Muevek | Device for making surface layer on work pieces moving longitudinally particularly by use of dusty integumentary material in cable industry |
| GB8923075D0 (en) * | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5849486A (en) | 1993-11-01 | 1998-12-15 | Nanogen, Inc. | Methods for hybridization analysis utilizing electrically controlled hybridization |
| US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| ES2082256T3 (es) | 1992-03-23 | 1996-03-16 | Hoffmann La Roche | Metodo de deteccion del adn. |
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| AU7662794A (en) | 1993-09-23 | 1995-04-10 | Zeneca Limited | Nucleic acid detection with energy transfer |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5491063A (en) * | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| DE69527125T2 (de) * | 1994-11-09 | 2003-02-27 | Hitachi, Ltd. | Verfahren und Vorrichtung zur DNS Analyse |
| US5858397A (en) * | 1995-10-11 | 1999-01-12 | University Of British Columbia | Liposomal formulations of mitoxantrone |
| EP2369007B1 (en) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9716052D0 (en) | 1996-12-06 | 1997-10-01 | Secr Defence | Reaction vessels |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| ATE273381T1 (de) * | 1997-02-12 | 2004-08-15 | Eugene Y Chan | Verfahren zur analyse von polymeren |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| GB9803382D0 (en) * | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| US6323337B1 (en) | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| KR100386606B1 (ko) * | 2001-02-03 | 2003-06-02 | 엘지전자 주식회사 | Ｄｎａ 검출 방법 및 그 장치 |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| KR100459394B1 (ko) * | 2001-10-30 | 2004-12-03 | 엘지전자 주식회사 | 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법 |
| AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| CA2500129C (en) * | 2002-09-30 | 2011-03-29 | F. Hoffmann-La Roche Ag | Oligonucleotides for genotyping thymidylate synthase gene |
| GB0223563D0 (en) | 2002-10-10 | 2002-11-20 | Secr Defence | Detection system |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| EP1694869A2 (en) * | 2003-11-10 | 2006-08-30 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2001
- 2001-05-25 GB GBGB0112868.5A patent/GB0112868D0/en not_active Ceased
-
2002
- 2002-05-24 DK DK02738330T patent/DK1390542T3/da active
- 2002-05-24 CA CA2448211A patent/CA2448211C/en not_active Expired - Fee Related
- 2002-05-24 EP EP02738330A patent/EP1390542B1/en not_active Expired - Lifetime
- 2002-05-24 AU AU2002311414A patent/AU2002311414B2/en not_active Ceased
- 2002-05-24 CN CNB028141334A patent/CN1304596C/zh not_active Expired - Fee Related
- 2002-05-24 ES ES02738330T patent/ES2240753T3/es not_active Expired - Lifetime
- 2002-05-24 AT AT02738330T patent/ATE298804T1/de not_active IP Right Cessation
- 2002-05-24 DE DE60204874T patent/DE60204874T2/de not_active Expired - Lifetime
- 2002-05-24 WO PCT/GB2002/002443 patent/WO2002097132A2/en not_active Ceased
- 2002-05-24 PT PT02738330T patent/PT1390542E/pt unknown
- 2002-05-24 KR KR10-2003-7015413A patent/KR20040024556A/ko not_active Withdrawn
- 2002-05-24 JP JP2003500296A patent/JP2004532044A/ja active Pending
- 2002-05-24 US US10/478,788 patent/US7700275B2/en not_active Expired - Fee Related
-
2010
- 2010-02-18 US US12/708,128 patent/US20100227326A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN1304596C (zh) | 2007-03-14 |
| DK1390542T3 (da) | 2005-08-08 |
| DE60204874T2 (de) | 2006-04-27 |
| HK1068651A1 (en) | 2005-04-29 |
| JP2004532044A (ja) | 2004-10-21 |
| US20040241679A1 (en) | 2004-12-02 |
| AU2002311414B2 (en) | 2006-11-09 |
| GB0112868D0 (en) | 2001-07-18 |
| ATE298804T1 (de) | 2005-07-15 |
| PT1390542E (pt) | 2005-11-30 |
| US20100227326A1 (en) | 2010-09-09 |
| EP1390542B1 (en) | 2005-06-29 |
| CA2448211C (en) | 2010-12-07 |
| CA2448211A1 (en) | 2002-12-05 |
| WO2002097132A3 (en) | 2003-09-12 |
| WO2002097132A2 (en) | 2002-12-05 |
| KR20040024556A (ko) | 2004-03-20 |
| EP1390542A2 (en) | 2004-02-25 |
| US7700275B2 (en) | 2010-04-20 |
| CN1527885A (zh) | 2004-09-08 |
| DE60204874D1 (de) | 2005-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2240753T3 (es) | Metodo de deteccion de acido nucleico. | |
| ES2232972T3 (es) | Sistema fluorimetrico de deteccion de un acido nucleico. | |
| ES2359322T3 (es) | Procedimiento para la amplificación de ácidos nucleicos. | |
| ES2183256T5 (es) | Dispositivo para la deteccion de reacciones de amplificacion de acidos nucleicos. | |
| ES2205572T3 (es) | Construcciones de cebador pcr para su utilizacion en un sistema integrado de señalizacion. | |
| CA2321185C (en) | Method for detection of target nucleic acids using pcr | |
| US7667024B2 (en) | Oligonucleotides labeled with a plurality of fluorophores | |
| ES2414286T3 (es) | Detección de ácidos nucleicos | |
| ES2549777T3 (es) | Métodos para analizar secuencias de ácido nucleico monocatenario | |
| PT1278889E (pt) | Farol de hibridização e método para a detecção e discriminação rápida de sequência | |
| JP2010046063A (ja) | Ｐｃｒ中のハイブリダイゼーションのモニタリング | |
| US20020197611A1 (en) | Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers | |
| AU2002311414A1 (en) | Nucleic acid detection method | |
| US20140274779A1 (en) | Multiplex allele detection | |
| JP2004532044A5 (enExample) | ||
| ES2281880T3 (es) | Procedimiento de deteccion de acidos nucleicos que usa multiples pares de fluoroforos donadores y moleculas inhibidoras de la fluorescencia en la misma sonda. | |
| ES2245315T3 (es) | Metodo de amplificacion para la deteccion de acidos nucleicos diana que implica transferencia de energia fluorescente. | |
| CN108531549A (zh) | 核酸分子检测方法及检测试剂盒 | |
| CN109477139A (zh) | 使用长ssDNA多核苷酸作为PCR测定中的引物的方法 | |
| JP6440997B2 (ja) | Ｐｃｒ法およびｐｃｒキット | |
| EP1606389B1 (en) | Methods for polynucleotide sequence detection | |
| KR101414714B1 (ko) | 경쟁적 증폭 반응을 이용하여 표적 핵산을 검출하는 방법 | |
| ES2360299T3 (es) | Baliza y método de hbridación para detección y discriminación rápida de secuencias. | |
| CN118272502A (zh) | 一种检测并分辨低频突变类型的方法 | |
| JP5568935B2 (ja) | 標的塩基配列の識別方法 |