JP2004532044A5 - - Google Patents
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- Publication number
- JP2004532044A5 JP2004532044A5 JP2003500296A JP2003500296A JP2004532044A5 JP 2004532044 A5 JP2004532044 A5 JP 2004532044A5 JP 2003500296 A JP2003500296 A JP 2003500296A JP 2003500296 A JP2003500296 A JP 2003500296A JP 2004532044 A5 JP2004532044 A5 JP 2004532044A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- label
- amplification
- nucleic acid
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims description 154
- 230000003321 amplification Effects 0.000 claims description 68
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 68
- 238000000034 method Methods 0.000 claims description 45
- 108020004414 DNA Proteins 0.000 claims description 42
- 238000006243 chemical reaction Methods 0.000 claims description 36
- 150000007523 nucleic acids Chemical group 0.000 claims description 34
- 239000011230 binding agent Substances 0.000 claims description 30
- 230000000295 complement effect Effects 0.000 claims description 23
- 108020004707 nucleic acids Proteins 0.000 claims description 20
- 102000039446 nucleic acids Human genes 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- 125000005647 linker group Chemical group 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 12
- 238000012544 monitoring process Methods 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 238000003752 polymerase chain reaction Methods 0.000 claims description 8
- 238000009396 hybridization Methods 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 6
- 108091033319 polynucleotide Proteins 0.000 claims description 6
- 102000040430 polynucleotide Human genes 0.000 claims description 6
- 239000002157 polynucleotide Substances 0.000 claims description 6
- 239000000975 dye Substances 0.000 claims description 5
- 239000000138 intercalating agent Substances 0.000 claims description 5
- 239000013043 chemical agent Substances 0.000 claims description 4
- 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 claims description 4
- 239000000155 melt Substances 0.000 claims description 4
- 230000001687 destabilization Effects 0.000 claims description 3
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 claims description 2
- 102000053602 DNA Human genes 0.000 claims description 2
- BDJDTKYGKHEMFF-UHFFFAOYSA-M QSY7 succinimidyl ester Chemical compound [Cl-].C=1C=C2C(C=3C(=CC=CC=3)S(=O)(=O)N3CCC(CC3)C(=O)ON3C(CCC3=O)=O)=C3C=C\C(=[N+](\C)C=4C=CC=CC=4)C=C3OC2=CC=1N(C)C1=CC=CC=C1 BDJDTKYGKHEMFF-UHFFFAOYSA-M 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 2
- 239000001022 rhodamine dye Substances 0.000 claims description 2
- 230000001404 mediated effect Effects 0.000 claims 4
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 11
- 238000002844 melting Methods 0.000 description 9
- 230000008018 melting Effects 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- IIRDTKBZINWQAW-UHFFFAOYSA-N hexaethylene glycol Chemical compound OCCOCCOCCOCCOCCOCCO IIRDTKBZINWQAW-UHFFFAOYSA-N 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- IPEHINMNWSMACL-UHFFFAOYSA-M 1-ethylquinolin-1-ium;perchlorate Chemical compound [O-]Cl(=O)(=O)=O.C1=CC=C2[N+](CC)=CC=CC2=C1 IPEHINMNWSMACL-UHFFFAOYSA-M 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010093628 Heterometrus granulomanus lectin Proteins 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- -1 for example Chemical compound 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0112868.5A GB0112868D0 (en) | 2001-05-25 | 2001-05-25 | Detection system |
| PCT/GB2002/002443 WO2002097132A2 (en) | 2001-05-25 | 2002-05-24 | Nucleic acid detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004532044A JP2004532044A (ja) | 2004-10-21 |
| JP2004532044A5 true JP2004532044A5 (enExample) | 2009-01-08 |
Family
ID=9915364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003500296A Pending JP2004532044A (ja) | 2001-05-25 | 2002-05-24 | 検出システム |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7700275B2 (enExample) |
| EP (1) | EP1390542B1 (enExample) |
| JP (1) | JP2004532044A (enExample) |
| KR (1) | KR20040024556A (enExample) |
| CN (1) | CN1304596C (enExample) |
| AT (1) | ATE298804T1 (enExample) |
| AU (1) | AU2002311414B2 (enExample) |
| CA (1) | CA2448211C (enExample) |
| DE (1) | DE60204874T2 (enExample) |
| DK (1) | DK1390542T3 (enExample) |
| ES (1) | ES2240753T3 (enExample) |
| GB (1) | GB0112868D0 (enExample) |
| PT (1) | PT1390542E (enExample) |
| WO (1) | WO2002097132A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| GB0223563D0 (en) * | 2002-10-10 | 2002-11-20 | Secr Defence | Detection system |
| ES2529204T3 (es) * | 2004-10-18 | 2015-02-17 | Brandeis University | Métodos para la amplificación de ácido nucleico |
| WO2006121277A1 (en) * | 2005-05-09 | 2006-11-16 | Korea Research Institute Of Standards And Science | Fret probes for fluorescent detection of the epsps gene |
| KR100764559B1 (ko) * | 2005-05-09 | 2007-10-08 | 한국표준과학연구원 | Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브 |
| CN100420943C (zh) * | 2005-06-23 | 2008-09-24 | 李卫 | Dna分子内杂交-聚丙烯酰胺凝胶电泳方法 |
| US8968700B2 (en) * | 2005-08-11 | 2015-03-03 | The Board Of Trustees Of The Leland Stanford Junior University | Imaging of protease activity in live cells using activity based probes |
| US7785786B2 (en) | 2006-01-23 | 2010-08-31 | Quest Diagnostics Investments Incorporated | Methods for detecting nucleic acids using multiple signals |
| CN100526461C (zh) * | 2006-07-10 | 2009-08-12 | 中国农业科学院棉花研究所 | 获得植物内含子序列扩增多态性的方法及其专用引物 |
| JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
| US8658366B2 (en) | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| US20100129796A1 (en) * | 2008-11-24 | 2010-05-27 | Micah Halpern | Dye probe fluorescence resonance energy transfer genotyping |
| GB201004339D0 (en) | 2010-03-16 | 2010-04-28 | Enigma Diagnostics Ltd | Sequence detection assay |
| GB201007868D0 (en) | 2010-05-11 | 2010-06-23 | Enigma Diagnostics Ltd | Sequence detection assay |
| WO2012118715A2 (en) | 2011-02-28 | 2012-09-07 | The Board Of Trustees Of Leland Stanford Junior University | Non-peptidic quenched fluorescent imaging probes |
| DE102011117520A1 (de) * | 2011-11-03 | 2013-05-08 | Frank Schleifenbaum | Sensorsystem für nicht intrusive Druckmessungen |
| WO2014145257A2 (en) | 2013-03-15 | 2014-09-18 | The Board Of Trustees Of The Leland Stanford Junior University | Activity-based probe compounds, compositions, and methods of use |
| EP3347714B1 (en) * | 2015-09-10 | 2021-03-10 | InSilixa Inc. | Methods for multiplex quantitative nucleic acid amplification |
| EP3510380B1 (en) | 2016-12-23 | 2023-11-08 | The Board of Trustees of the Leland Stanford Junior University | Activity-based probe compounds, compositions, and methods of use |
| EP3601261A4 (en) | 2017-03-30 | 2021-07-14 | The Board of Trustees of the Leland Stanford Junior University | CONTRAST AGENTS ACTIVATED BY A PROTEASE FOR IN VIVO IMAGING |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1533151A (fr) * | 1962-05-18 | 1968-07-19 | Rhone Poulenc Sa | Nouvel antibiotique et sa préparation |
| US4012284A (en) * | 1962-11-16 | 1977-03-15 | Societa' Farmaceutici Italia, S.p.A. | Process of preparation of antibiotic F.I. 1762 derivatives |
| US3183157A (en) * | 1963-02-01 | 1965-05-11 | Upjohn Co | Antibiotic nogalamycin and method of producing |
| US4197249A (en) * | 1977-08-15 | 1980-04-08 | American Cyanamid Company | 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| CA1219824A (en) | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| HU201626B (en) | 1989-09-05 | 1990-11-28 | Magyar Kabel Muevek | Device for making surface layer on work pieces moving longitudinally particularly by use of dusty integumentary material in cable industry |
| GB8923075D0 (en) * | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5849486A (en) | 1993-11-01 | 1998-12-15 | Nanogen, Inc. | Methods for hybridization analysis utilizing electrically controlled hybridization |
| US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| ES2082256T3 (es) | 1992-03-23 | 1996-03-16 | Hoffmann La Roche | Metodo de deteccion del adn. |
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| AU7662794A (en) | 1993-09-23 | 1995-04-10 | Zeneca Limited | Nucleic acid detection with energy transfer |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5491063A (en) * | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| DE69527125T2 (de) * | 1994-11-09 | 2003-02-27 | Hitachi, Ltd. | Verfahren und Vorrichtung zur DNS Analyse |
| US5858397A (en) * | 1995-10-11 | 1999-01-12 | University Of British Columbia | Liposomal formulations of mitoxantrone |
| EP2369007B1 (en) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9716052D0 (en) | 1996-12-06 | 1997-10-01 | Secr Defence | Reaction vessels |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| ATE273381T1 (de) * | 1997-02-12 | 2004-08-15 | Eugene Y Chan | Verfahren zur analyse von polymeren |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| GB9803382D0 (en) * | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| US6323337B1 (en) | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| KR100386606B1 (ko) * | 2001-02-03 | 2003-06-02 | 엘지전자 주식회사 | Ｄｎａ 검출 방법 및 그 장치 |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| KR100459394B1 (ko) * | 2001-10-30 | 2004-12-03 | 엘지전자 주식회사 | 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법 |
| AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| CA2500129C (en) * | 2002-09-30 | 2011-03-29 | F. Hoffmann-La Roche Ag | Oligonucleotides for genotyping thymidylate synthase gene |
| GB0223563D0 (en) | 2002-10-10 | 2002-11-20 | Secr Defence | Detection system |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| EP1694869A2 (en) * | 2003-11-10 | 2006-08-30 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2001
- 2001-05-25 GB GBGB0112868.5A patent/GB0112868D0/en not_active Ceased
-
2002
- 2002-05-24 DK DK02738330T patent/DK1390542T3/da active
- 2002-05-24 CA CA2448211A patent/CA2448211C/en not_active Expired - Fee Related
- 2002-05-24 EP EP02738330A patent/EP1390542B1/en not_active Expired - Lifetime
- 2002-05-24 AU AU2002311414A patent/AU2002311414B2/en not_active Ceased
- 2002-05-24 CN CNB028141334A patent/CN1304596C/zh not_active Expired - Fee Related
- 2002-05-24 ES ES02738330T patent/ES2240753T3/es not_active Expired - Lifetime
- 2002-05-24 AT AT02738330T patent/ATE298804T1/de not_active IP Right Cessation
- 2002-05-24 DE DE60204874T patent/DE60204874T2/de not_active Expired - Lifetime
- 2002-05-24 WO PCT/GB2002/002443 patent/WO2002097132A2/en not_active Ceased
- 2002-05-24 PT PT02738330T patent/PT1390542E/pt unknown
- 2002-05-24 KR KR10-2003-7015413A patent/KR20040024556A/ko not_active Withdrawn
- 2002-05-24 JP JP2003500296A patent/JP2004532044A/ja active Pending
- 2002-05-24 US US10/478,788 patent/US7700275B2/en not_active Expired - Fee Related
-
2010
- 2010-02-18 US US12/708,128 patent/US20100227326A1/en not_active Abandoned
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