DE60204874T2 - Verfahren zur nachweis von nukleinsäuren - Google Patents

Verfahren zur nachweis von nukleinsäuren Download PDF

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Publication number
DE60204874T2
DE60204874T2 DE60204874T DE60204874T DE60204874T2 DE 60204874 T2 DE60204874 T2 DE 60204874T2 DE 60204874 T DE60204874 T DE 60204874T DE 60204874 T DE60204874 T DE 60204874T DE 60204874 T2 DE60204874 T2 DE 60204874T2
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DE
Germany
Prior art keywords
probe
label
amplification
fluorescence
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60204874T
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German (de)
English (en)
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DE60204874D1 (de
Inventor
Martin Alan Salisbury LEE
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UK Secretary of State for Defence
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UK Secretary of State for Defence
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Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Application granted granted Critical
Publication of DE60204874D1 publication Critical patent/DE60204874D1/de
Publication of DE60204874T2 publication Critical patent/DE60204874T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
DE60204874T 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren Expired - Lifetime DE60204874T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0112868.5A GB0112868D0 (en) 2001-05-25 2001-05-25 Detection system
GB0112868 2001-05-25
PCT/GB2002/002443 WO2002097132A2 (en) 2001-05-25 2002-05-24 Nucleic acid detection method

Publications (2)

Publication Number Publication Date
DE60204874D1 DE60204874D1 (de) 2005-08-04
DE60204874T2 true DE60204874T2 (de) 2006-04-27

Family

ID=9915364

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60204874T Expired - Lifetime DE60204874T2 (de) 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren

Country Status (14)

Country Link
US (2) US7700275B2 (enExample)
EP (1) EP1390542B1 (enExample)
JP (1) JP2004532044A (enExample)
KR (1) KR20040024556A (enExample)
CN (1) CN1304596C (enExample)
AT (1) ATE298804T1 (enExample)
AU (1) AU2002311414B2 (enExample)
CA (1) CA2448211C (enExample)
DE (1) DE60204874T2 (enExample)
DK (1) DK1390542T3 (enExample)
ES (1) ES2240753T3 (enExample)
GB (1) GB0112868D0 (enExample)
PT (1) PT1390542E (enExample)
WO (1) WO2002097132A2 (enExample)

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DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen

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CN100420943C (zh) * 2005-06-23 2008-09-24 李卫 Dna分子内杂交-聚丙烯酰胺凝胶电泳方法
US8968700B2 (en) * 2005-08-11 2015-03-03 The Board Of Trustees Of The Leland Stanford Junior University Imaging of protease activity in live cells using activity based probes
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CN100526461C (zh) * 2006-07-10 2009-08-12 中国农业科学院棉花研究所 获得植物内含子序列扩增多态性的方法及其专用引物
JP2009044967A (ja) * 2007-08-14 2009-03-05 Sony Corp 二本鎖核酸の形成に係る情報を得る方法
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
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WO2012118715A2 (en) 2011-02-28 2012-09-07 The Board Of Trustees Of Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
WO2014145257A2 (en) 2013-03-15 2014-09-18 The Board Of Trustees Of The Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
EP3347714B1 (en) * 2015-09-10 2021-03-10 InSilixa Inc. Methods for multiplex quantitative nucleic acid amplification
EP3510380B1 (en) 2016-12-23 2023-11-08 The Board of Trustees of the Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
EP3601261A4 (en) 2017-03-30 2021-07-14 The Board of Trustees of the Leland Stanford Junior University CONTRAST AGENTS ACTIVATED BY A PROTEASE FOR IN VIVO IMAGING

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen

Also Published As

Publication number Publication date
CN1304596C (zh) 2007-03-14
DK1390542T3 (da) 2005-08-08
HK1068651A1 (en) 2005-04-29
JP2004532044A (ja) 2004-10-21
ES2240753T3 (es) 2005-10-16
US20040241679A1 (en) 2004-12-02
AU2002311414B2 (en) 2006-11-09
GB0112868D0 (en) 2001-07-18
ATE298804T1 (de) 2005-07-15
PT1390542E (pt) 2005-11-30
US20100227326A1 (en) 2010-09-09
EP1390542B1 (en) 2005-06-29
CA2448211C (en) 2010-12-07
CA2448211A1 (en) 2002-12-05
WO2002097132A3 (en) 2003-09-12
WO2002097132A2 (en) 2002-12-05
KR20040024556A (ko) 2004-03-20
EP1390542A2 (en) 2004-02-25
US7700275B2 (en) 2010-04-20
CN1527885A (zh) 2004-09-08
DE60204874D1 (de) 2005-08-04

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