ES2176336T3 - Cabadores especificos y universales de amplificacion para detectar e identificar de forma rapida bacterias patogenas comunes y genes de resistencia a antibioticos de especimenes clinicos para diagnostico rutinario en laboratorios de microbiologia. - Google Patents

Cabadores especificos y universales de amplificacion para detectar e identificar de forma rapida bacterias patogenas comunes y genes de resistencia a antibioticos de especimenes clinicos para diagnostico rutinario en laboratorios de microbiologia.

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Publication number
ES2176336T3
ES2176336T3 ES95931109T ES95931109T ES2176336T3 ES 2176336 T3 ES2176336 T3 ES 2176336T3 ES 95931109 T ES95931109 T ES 95931109T ES 95931109 T ES95931109 T ES 95931109T ES 2176336 T3 ES2176336 T3 ES 2176336T3
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Spain
Prior art keywords
bacterial
antibiotic resistance
specific
clinical specimens
pathogene
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Expired - Lifetime
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ES95931109T
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English (en)
Inventor
Michel G Bergeron
Marc Ouellette
Paul H Roy
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Infectio Diagnostic IDI Inc
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Infectio Diagnostic IDI Inc
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Application filed by Infectio Diagnostic IDI Inc filed Critical Infectio Diagnostic IDI Inc
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Publication of ES2176336T3 publication Critical patent/ES2176336T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C07K14/212—Moraxellaceae, e.g. Acinetobacter, Moraxella, Oligella, Psychrobacter
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26—Klebsiella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Pulmonology (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A METODOS BASADOS EN EL ADN PARA UNA DETECCION BACTERIANA UNIVERSAL, PARA UNA DETECCION ESPECIFICA DE LA PNEUMONIAE, PSEUDOMONAS AERUGINOS, PROTEUS MIRABILIS, STREPTOCOCCUS PNEUMONIAE, STAPHYLOCOCCUS AUREUS, STAPHYLOCOCCUS EPIDERMINIS, ENTEROCOCCUS FAECALIS, STAPHYLOCOCCUS SAPROPHYTICUS, STREPTOCOCCUS PYOGENES, HAEMOPHILUS INFLUENZAE Y MORAXELA CATARRHALIS, ASI COMO LA DETECCION ESPECIFICA DIRECTA DE LOS GENES NORMALMENTE ENCONTRADOS Y DE GENES DE RESISTENCIA A LOS ANTIBIOTICOS BACTERIANOS CLINICAMENTE RELEVANTES A PARTIR DE EN MUESTRAS CLINICAS O, ALTERNATIVAMENTE, EN UNA COLONIA BACTERIANA. LAS ESPECIES BACTERIANAS ANTEDICHAS PUEDEN REPRESENTAR HASTA EL 80 % DE LOS PATOGENOS BACTERIANOS AISLADOS EN LABORATORIOS MICROBIOLOGIA RUTINARIOS. EL NUCLEO DE ESTA INVENCION CONSISTE PRINCIPALMENTE EN LAS SECUENCIAS DE ADN DE TODOS LOS FRAGMENTOS DE ADN GENOMICOS ESPECIFICOS DE ESPECIES SELECCIONADOS MEDIANTE LA HIBRIDACION DE LIBRERIAS GENOMICAS O, ALTERNATIVAMENTE,SELECCIONADOS DE LOS DATOS DE BANCOS ASI COMO EN CUALQUIERA EN LAS SECUENCIAS DE OLIGONUCLEOTIDOS DERIVADAS DE ESTAS SECUENCIAS QUE SE PUEDEN UTILIZAR COMO SONDAS O IMPRIMADORES DE AMPLIFICACION PARA PCR O CUALQUIER OTRO METODO DE AMPLIFICACION DE ACIDOS NUCLEICOS. EN LA INVENCION TAMBIEN SE PRESENTAN SECUENCIAS DE ADN DE LOS GENES DE RESISTENCIA A LOS ANTIBIOTICOS CLINICAMENTE RELEVANTES SELECCIONADOS.
ES95931109T 1994-09-12 1995-09-12 Cabadores especificos y universales de amplificacion para detectar e identificar de forma rapida bacterias patogenas comunes y genes de resistencia a antibioticos de especimenes clinicos para diagnostico rutinario en laboratorios de microbiologia. Expired - Lifetime ES2176336T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US30473294A 1994-09-12 1994-09-12

Publications (1)

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ES2176336T3 true ES2176336T3 (es) 2002-12-01

Family

ID=23177744

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95931109T Expired - Lifetime ES2176336T3 (es) 1994-09-12 1995-09-12 Cabadores especificos y universales de amplificacion para detectar e identificar de forma rapida bacterias patogenas comunes y genes de resistencia a antibioticos de especimenes clinicos para diagnostico rutinario en laboratorios de microbiologia.

Country Status (14)

Country Link
US (3) US6001564A (es)
EP (2) EP1138786B1 (es)
JP (2) JP4176146B2 (es)
AT (1) ATE219524T1 (es)
BR (1) BR9508918A (es)
CA (1) CA2199144C (es)
DE (1) DE69527154T2 (es)
DK (1) DK0804616T3 (es)
ES (1) ES2176336T3 (es)
MX (1) MX9701847A (es)
NO (1) NO971111L (es)
NZ (1) NZ292494A (es)
PT (1) PT804616E (es)
WO (1) WO1996008582A2 (es)

Families Citing this family (156)

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