ES2170622A1 - Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. - Google Patents

Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.

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Publication number
ES2170622A1
ES2170622A1 ES009902673A ES9902673A ES2170622A1 ES 2170622 A1 ES2170622 A1 ES 2170622A1 ES 009902673 A ES009902673 A ES 009902673A ES 9902673 A ES9902673 A ES 9902673A ES 2170622 A1 ES2170622 A1 ES 2170622A1
Authority
ES
Spain
Prior art keywords
nucleic acid
infective
virus
directing
formation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009902673A
Other languages
English (en)
Other versions
ES2170622B1 (es
Inventor
Sanchez Luis Enjuanes
Toral Fernando Almazan
Martinez J Manuel Gonzalez
Permisan Ander Izeta
Zoltan Penzes
Villanueva Sara Alonso
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8310818&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2170622(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES009902673A priority Critical patent/ES2170622B1/es
Priority to DE60010921T priority patent/DE60010921T3/de
Priority to PL356390A priority patent/PL206816B1/pl
Priority to KR1020027007118A priority patent/KR100755814B1/ko
Priority to NZ518990A priority patent/NZ518990A/en
Priority to CNA2004100789786A priority patent/CN1616670A/zh
Priority to JP2001541529A priority patent/JP4637432B2/ja
Priority to MXPA02005525A priority patent/MXPA02005525A/es
Priority to AU30051/01A priority patent/AU785081B2/en
Priority to TR2004/01186T priority patent/TR200401186T4/xx
Priority to SK761-2002A priority patent/SK7612002A3/sk
Priority to IL14985900A priority patent/IL149859A0/xx
Priority to AT00990628T priority patent/ATE267246T1/de
Priority to HU0203305A priority patent/HU228205B1/hu
Priority to YUP-375/02A priority patent/RS50372B/sr
Priority to ES00990628T priority patent/ES2218276T5/es
Priority to CNB008165904A priority patent/CN100402643C/zh
Priority to US10/148,669 priority patent/US7445928B2/en
Priority to CA002393325A priority patent/CA2393325A1/en
Priority to EP00990628A priority patent/EP1234024B2/en
Priority to EP04007406A priority patent/EP1437400A3/en
Priority to BR0016145-4A priority patent/BR0016145A/pt
Priority to PT00990628T priority patent/PT1234024E/pt
Priority to DK00990628.0T priority patent/DK1234024T4/da
Priority to CNA2006100733455A priority patent/CN1872994A/zh
Priority to CZ20021882A priority patent/CZ302785B6/cs
Priority to PCT/EP2000/012063 priority patent/WO2001039797A2/en
Priority to SI200030394T priority patent/SI1234024T2/sl
Priority to ARP000106406A priority patent/AR027893A1/es
Priority to ZA200203560A priority patent/ZA200203560B/xx
Priority to NO20022419A priority patent/NO331812B1/no
Priority to IL149859A priority patent/IL149859A/en
Publication of ES2170622A1 publication Critical patent/ES2170622A1/es
Priority to US10/238,786 priority patent/US7368557B2/en
Publication of ES2170622B1 publication Critical patent/ES2170622B1/es
Application granted granted Critical
Priority to AU2006203327A priority patent/AU2006203327B2/en
Priority to US12/285,213 priority patent/US20100167352A1/en
Priority to US12/938,771 priority patent/US20110142879A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/215—Coronaviridae, e.g. avian infectious bronchitis virus
    • A61K39/225—Porcine transmissible gastroenteritis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • A61K2039/5256—Virus expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
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    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/20043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Gastroenterology & Hepatology (AREA)
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Abstract

Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. El clon infectivo derivado de un coronavirus comprende un cDNA que codifica el gRMA de un coronavirus clonado bajo una secuencia promotora de la transcripción. El vector viral recombinante comprende un clon infectivo modificado para contener un ácido nucleico heterólogo insertado en dicho clon infectivo bajo condiciones que permiten la expresión de dicho ácido nucleico heterólogo. Los clones y vectores infectivos son útiles tanto en investigación básica como aplicada, en el desarrollo de sistemas de expresión eficientes de productos de interés (proteínas, enzimas, anticuerpos, etc.), vectores vacunales y terapia génica.
ES009902673A 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. Expired - Fee Related ES2170622B1 (es)

Priority Applications (36)

Application Number Priority Date Filing Date Title
ES009902673A ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
BR0016145-4A BR0016145A (pt) 1999-12-03 2000-11-30 Clones infecciosos
YUP-375/02A RS50372B (sr) 1999-12-03 2000-11-30 Infektivni klonovi
KR1020027007118A KR100755814B1 (ko) 1999-12-03 2000-11-30 감염성 클론
NZ518990A NZ518990A (en) 1999-12-03 2000-11-30 Use of bacterial artificial chromosomes (BACs) to prepare heterologous DNA expression vectors to formulate vaccines
CNA2004100789786A CN1616670A (zh) 1999-12-03 2000-11-30 感染性克隆
JP2001541529A JP4637432B2 (ja) 1999-12-03 2000-11-30 感染性クローン
EP00990628A EP1234024B2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
AU30051/01A AU785081B2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant RNA-virus
TR2004/01186T TR200401186T4 (tr) 1999-12-03 2000-11-30 Rekombinant bir RNA-virüs oluşumunu yönlendirebilen nükleik asit sekansları içerikli suni kromozom yapıları.
SK761-2002A SK7612002A3 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
IL14985900A IL149859A0 (en) 1999-12-03 2000-11-30 Infectious clones
AT00990628T ATE267246T1 (de) 1999-12-03 2000-11-30 Künstliche chromosomen, die die genetische information für die bildung von rekombinantem rna-virus enthalten
HU0203305A HU228205B1 (en) 1999-12-03 2000-11-30 Infectious clones
PL356390A PL206816B1 (pl) 1999-12-03 2000-11-30 Sposób otrzymywania DNA, klon zakaźny, E.coli, rekombinowany wektor wirusowy i szczepionka do ochrony zwierzęcia
ES00990628T ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.
CNB008165904A CN100402643C (zh) 1999-12-03 2000-11-30 感染性克隆
US10/148,669 US7445928B2 (en) 1999-12-03 2000-11-30 Bacterial artificial chromosome construct encoding recombinant coronavirus
DE60010921T DE60010921T3 (de) 1999-12-03 2000-11-30 Künstliche chromosomen, die die genetische information für die bildung von rekombinantem rna-virus enthalten
MXPA02005525A MXPA02005525A (es) 1999-12-03 2000-11-30 Clones infecciosos.
EP04007406A EP1437400A3 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
CA002393325A CA2393325A1 (en) 1999-12-03 2000-11-30 Infectious clones
PT00990628T PT1234024E (pt) 1999-12-03 2000-11-30 Construcoes de cromossomas artificiais contendo sequencias de acido nucleico capazes de dirigir a formacao de um virus de rna recombinante
DK00990628.0T DK1234024T4 (da) 1999-12-03 2000-11-30 Kunstige chromosomkonstruktioner, der indeholder nucleinsyresekvenser, som er i stand til at dirigere dannelsen af et rekombinant RNA-virus
CNA2006100733455A CN1872994A (zh) 1999-12-03 2000-11-30 感染性克隆
CZ20021882A CZ302785B6 (cs) 1999-12-03 2000-11-30 Zpusob prípravy DNA odvozené od koronaviru, príslušné infekcní klony, vektory a vakcíny
PCT/EP2000/012063 WO2001039797A2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
SI200030394T SI1234024T2 (sl) 1999-12-03 2000-11-30 Umetni kromosomi, ki vsebujejo sekvence nukleinskih kislin, sposobne usmerjanja tvorbe rekombinantnega rna virusa
ARP000106406A AR027893A1 (es) 1999-12-03 2000-12-04 Clones infecciosos
ZA200203560A ZA200203560B (en) 1999-12-03 2002-05-06 Infectious clones.
NO20022419A NO331812B1 (no) 1999-12-03 2002-05-22 Fremgangsmate for a fremstille DNA avledet fra coronavirus samt infeksiose kloner, rekombinant viral vektor samt vaksine
IL149859A IL149859A (en) 1999-12-03 2002-05-27 Tribes of pollutants
US10/238,786 US7368557B2 (en) 1999-12-03 2002-09-11 Polynucleotides encoding porcine transmissible gastroenteritis virus
AU2006203327A AU2006203327B2 (en) 1999-12-03 2006-08-03 Infectious clones
US12/285,213 US20100167352A1 (en) 1999-12-03 2008-09-30 Infectious clones
US12/938,771 US20110142879A1 (en) 1999-12-03 2010-11-03 Infectious clones

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009902673A ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.

Publications (2)

Publication Number Publication Date
ES2170622A1 true ES2170622A1 (es) 2002-08-01
ES2170622B1 ES2170622B1 (es) 2004-05-16

Family

ID=8310818

Family Applications (2)

Application Number Title Priority Date Filing Date
ES009902673A Expired - Fee Related ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
ES00990628T Expired - Lifetime ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES00990628T Expired - Lifetime ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.

Country Status (27)

Country Link
US (4) US7445928B2 (es)
EP (2) EP1437400A3 (es)
JP (1) JP4637432B2 (es)
KR (1) KR100755814B1 (es)
CN (3) CN1616670A (es)
AR (1) AR027893A1 (es)
AT (1) ATE267246T1 (es)
AU (2) AU785081B2 (es)
BR (1) BR0016145A (es)
CA (1) CA2393325A1 (es)
CZ (1) CZ302785B6 (es)
DE (1) DE60010921T3 (es)
DK (1) DK1234024T4 (es)
ES (2) ES2170622B1 (es)
HU (1) HU228205B1 (es)
IL (2) IL149859A0 (es)
MX (1) MXPA02005525A (es)
NO (1) NO331812B1 (es)
NZ (1) NZ518990A (es)
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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2170622B1 (es) 1999-12-03 2004-05-16 Consejo Superior De Investigaciones Cientificas Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
US7556957B2 (en) 2001-05-17 2009-07-07 Stichting Voor De Technische Wetenschappen Coronavirus-like particles comprising functionally deleted genomes
MXPA03010427A (es) * 2001-05-17 2004-12-06 Univ Utrecht Particulas del tipo del virus corona que comprenden genomas funcionalmente eliminados.
US7906311B2 (en) 2002-03-20 2011-03-15 Merial Limited Cotton rat lung cells for virus culture
US7371837B2 (en) * 2004-07-21 2008-05-13 The University Of Hong Kong Human virus causing respiratory tract infection and uses thereof
EP1619246A1 (en) * 2004-07-23 2006-01-25 Université de la Méditerranée, Aix-Marseille II RNA dependent RNA polymerases from coronavirus and their use in molecular biology and drug screening
EP1632247A1 (en) * 2004-09-03 2006-03-08 Consejo Superior De Investigaciones Cientificas Nucleic acid sequences encoding FMDV proteins capable of associating into a virus-like particle
EP2295590A1 (en) 2004-09-03 2011-03-16 Consejo Superior De Investigaciones Cientificas Nucleic acid sequences encoding proteins capable of associating into a virus-like particle
KR20070072608A (ko) 2004-10-22 2007-07-04 레비비코르 인코포레이션 유전자 변형된 면역계를 가지는 유제류
EP1792996A1 (en) 2005-12-01 2007-06-06 Consejo Superior de Investigaciones Cientificas Nucleic acid sequences encoding vaccines against Porcine reproductive and respiratory syndrome virus (PRRSV)
JP2007312649A (ja) * 2006-05-24 2007-12-06 Japan Health Science Foundation 再構築された感染性レトロウイルスゲノムクローンｄｎａ及びそれを含む微生物ならびにそれらの製造方法
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
FR2947839A1 (fr) * 2009-07-09 2011-01-14 Centre Nat Rech Scient Nouveaux agents antibacteriens
EP2681232A1 (en) 2011-03-02 2014-01-08 Intervet International B.V. Infectious bronchitis virus (ibv) spike protein as subunit vaccine
GB201307528D0 (en) * 2013-04-26 2013-06-12 Univ Leuven Kath Bacterial artificial chromosomes
CN106459998A (zh) * 2014-05-16 2017-02-22 耶鲁大学 预防或治疗慢性乙型肝炎病毒(hbv)感染的基于病毒样囊泡(vlv)的疫苗
JP2017515509A (ja) * 2014-05-16 2017-06-15 イエール ユニバーシティ ワクチン用途のための高力価ウイルス様ベシクルの進化の方法
KR20170057384A (ko) 2014-09-22 2017-05-24 리젠츠 오브 더 유니버시티 오브 미네소타 피친데 바이러스 역유전학 시스템 및 이용 방법
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003400A1 (en) * 1993-07-23 1995-02-02 Johns Hopkins University School Of Medicine Recombinationally targeted cloning in yeast artificial chromosomes
WO1997034008A1 (es) * 1996-03-14 1997-09-18 Cyanamid Iberica, S.A. Vectores basados en genomas virales defectivos recombinantes y su empleo en la formulacion de vacunas
WO1999006582A1 (de) * 1997-08-01 1999-02-11 Koszinowski Ulrich H Rekombinanter vektor, der infektiöse virale genomsequenzen mit einer grösse von mehr als 100 kb enthält, verfahren zu seiner herstellung und verwendung zur mutagenese der viralen sequenzen
WO1999043842A1 (en) * 1998-02-26 1999-09-02 Neurovir, Inc. Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant virus

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5833975A (en) * 1989-03-08 1998-11-10 Virogenetics Corporation Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence
US5288641A (en) 1984-06-04 1994-02-22 Arch Development Corporation Herpes Simplex virus as a vector
US5550289A (en) * 1985-01-07 1996-08-27 Syntex (U.S.A.) Inc. N-(1,(1-1)-dialkyloxy)-and N-(1,(1-1)-dialkenyloxy alk-1-yl-N-N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) * 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5106733A (en) * 1987-06-25 1992-04-21 Immunex Corporation Bovine granulocyte-macrophage colony stimulating factor
WO1990009441A1 (en) 1989-02-01 1990-08-23 The General Hospital Corporation Herpes simplex virus type i expression vector
US5811103A (en) * 1989-03-19 1998-09-22 Akzo Nobel N.V. Hog cholera virus vaccine and diagnostic
US5703055A (en) * 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
CA2039921A1 (en) 1990-04-16 1991-10-17 Xandra O. Breakefield Transfer and expression of gene sequences into central nervous system cells using herpes simplex virus mutants with deletions in genes for viral replication
US5264618A (en) * 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
AU672359B2 (en) * 1991-03-07 1996-10-03 Virogenetics Corporation Genetically engineered vaccine strain
ES2026827A6 (es) * 1991-03-26 1992-05-01 Ercros Sa Procedimiento para la produccion de una vacuna subunidad contra el parvovirus porcino.
US5382425A (en) * 1992-01-13 1995-01-17 Syntro Corporation Recombinant swinepox virus
US6033904A (en) * 1992-01-13 2000-03-07 Syntro Corporation Recombinant swinepox virus
US5658724A (en) 1992-07-31 1997-08-19 University Of Pittsburgh Of The Commonwealth System Of Higher Education Herpes simplex virus strains deficient for the essential immediate early genes ICP4 and ICP27 and methods for their production, growth and use
ES2152304T3 (es) * 1993-02-08 2001-02-01 Bayer Ag Procedimiento para el crecimiento del virus del sindrome reproductivo y respiratorio porcino y su uso en vacunas.
US5910488A (en) * 1993-06-07 1999-06-08 Vical Incorporated Plasmids suitable for gene therapy
US20020146392A1 (en) 1993-06-24 2002-10-10 Frank L. Graham Helper dependent adenovirus vectors based on site-specific recombinases
US6015686A (en) * 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
EP0804249A2 (en) * 1994-03-15 1997-11-05 Brown University Research Foundation Polymeric gene delivery system
AU694519B2 (en) * 1994-04-29 1998-07-23 Immuno Aktiengesellschaft Recombinant poxviruses with foreign polynucleotides in essential regions
GB2289279B (en) * 1994-05-13 1998-09-16 Iberica Cyanamid Diagnostic kits and vaccines containing recombinant PRRSV proteins
US5585096A (en) 1994-06-23 1996-12-17 Georgetown University Replication-competent herpes simplex virus mediates destruction of neoplastic cells
GB9415319D0 (en) 1994-07-29 1994-09-21 Medical Res Council HSV viral vector
WO1996015779A1 (en) 1994-11-21 1996-05-30 The Trustees Of Columbia University In The City Of New York Unique associated kaposi's sarcoma virus sequences and uses thereof
US5719131A (en) * 1994-12-09 1998-02-17 Genzyme Corporation Cationic amphiphiles containing dialkylamine lipophilic groups for intracellular delivery of therapeutic molecules
ES2264136T3 (es) 1995-02-21 2006-12-16 Cantab Pharmaceuticals Research Limited Preparaciones virales, vectores, inmunogenos y sus vacunas.
US6043232A (en) * 1997-07-23 2000-03-28 Nitromed, Inc. Nitroso esters of beta-oxo-amides and aryl propionic acid derivatives of non-steroidal antiinflammatory drugs
US5705385A (en) * 1995-06-07 1998-01-06 Inex Pharmaceuticals Corporation Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
US5820869A (en) * 1995-06-07 1998-10-13 American Home Products Corporation Recombinant raccoon pox viruses and their use as an effective vaccine against feline immunodeficiency virus infection
US6019980A (en) * 1995-06-07 2000-02-01 Connaught Laboratories Limited Nucleic acid respiratory syncytial virus vaccines
US5851826A (en) 1995-07-26 1998-12-22 Children's Medical Center Corporation Helper virus-free herpesvirus vector packaging system
US5795872A (en) * 1995-09-19 1998-08-18 Pharmadigm, Inc. DNA construct for immunization
US6133243A (en) 1996-02-22 2000-10-17 Onyx Pharmaceuticals, Inc. Liposomal-viral DNA complexes for treating disease
FR2751224B1 (fr) * 1996-07-19 1998-11-20 Rhone Merieux Formule de vaccin polynucleotidique contre les pathologies respiratoires et de reproduction des porcs
US6004777A (en) * 1997-03-12 1999-12-21 Virogenetics Corporation Vectors having enhanced expression, and methods of making and uses thereof
US5990091A (en) * 1997-03-12 1999-11-23 Virogenetics Corporation Vectors having enhanced expression, and methods of making and uses thereof
FR2781159B1 (fr) * 1998-07-06 2000-10-06 Merial Sas Vaccin circovirus et parvovirus porcin
US6391314B1 (en) * 1997-10-03 2002-05-21 Merial Porcine circoviruses vaccines diagnostic reagents
US7211379B2 (en) * 1997-10-03 2007-05-01 Merial Sas Prevention of myocarditis, abortion and intrauterine infection associated with porcine circovirus-2
UA78180C2 (uk) * 1997-10-03 2007-03-15 Меріаль Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти
US6517843B1 (en) * 1999-08-31 2003-02-11 Merial Reduction of porcine circovirus-2 viral load with inactivated PCV-2
US6165493A (en) * 1997-10-22 2000-12-26 New York Blood Center, Inc. "Methods and compositions for decreasing the frequency of HIV, herpesvirus and sexually transmitted bacterial infections"
US20040062775A1 (en) * 1997-12-05 2004-04-01 Agence Francaise De Securite Sanitaire Des Aliments Circovirus sequences associated with piglet weight loss disease (PWD)
FR2772047B1 (fr) * 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
RU2000118777A (ru) * 1997-12-11 2002-07-10 Университи Оф Саскачеван (Ca) Вирус синдрома мультисистемного истощения свиньи у поросят-отъемышей
WO1999045956A1 (en) * 1998-03-13 1999-09-16 University Of Georgia Research Foundation, Inc. Vaccines against circovirus infections
US6497883B1 (en) * 1999-06-10 2002-12-24 Merial Porcine circovirus recombinant poxvirus vaccine
ES2170622B1 (es) 1999-12-03 2004-05-16 Consejo Superior De Investigaciones Cientificas Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
EP1852511B1 (en) 1999-12-14 2010-02-17 Novartis AG Bovine immunodeficiency virus (biv) based vectors
WO2001090340A2 (en) 2000-05-21 2001-11-29 University Of North Carolina At Chapel Hill Assembly of large viral genomes and chromosomes from subclones
DE10044648A1 (de) * 2000-09-08 2002-03-21 Aventis Behring Gmbh Verfahren zur Vermehrung oder zur Entfernung von Cirocoviren aus biologischem Material
DK1379671T3 (da) * 2001-03-27 2009-06-29 Univ Saskatchewan Fremgangsmåder til dyrkning af circovirus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003400A1 (en) * 1993-07-23 1995-02-02 Johns Hopkins University School Of Medicine Recombinationally targeted cloning in yeast artificial chromosomes
WO1997034008A1 (es) * 1996-03-14 1997-09-18 Cyanamid Iberica, S.A. Vectores basados en genomas virales defectivos recombinantes y su empleo en la formulacion de vacunas
WO1999006582A1 (de) * 1997-08-01 1999-02-11 Koszinowski Ulrich H Rekombinanter vektor, der infektiöse virale genomsequenzen mit einer grösse von mehr als 100 kb enthält, verfahren zu seiner herstellung und verwendung zur mutagenese der viralen sequenzen
WO1999043842A1 (en) * 1998-02-26 1999-09-02 Neurovir, Inc. Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant virus

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