ES2131489T1 - Metodo para la produccion de nuevos biopolimeros de poliester. - Google Patents

Metodo para la produccion de nuevos biopolimeros de poliester.

Info

Publication number
ES2131489T1
ES2131489T1 ES97203215T ES97203215T ES2131489T1 ES 2131489 T1 ES2131489 T1 ES 2131489T1 ES 97203215 T ES97203215 T ES 97203215T ES 97203215 T ES97203215 T ES 97203215T ES 2131489 T1 ES2131489 T1 ES 2131489T1
Authority
ES
Spain
Prior art keywords
phb
polymers
pha
production
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES97203215T
Other languages
English (en)
Other versions
ES2131489T3 (es
Inventor
Oliver P Peoples
Anthony J Sinskey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23491962&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2131489(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of ES2131489T1 publication Critical patent/ES2131489T1/es
Application granted granted Critical
Publication of ES2131489T3 publication Critical patent/ES2131489T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA CONTROLAR Y MODIFICAR LA SINTESIS DE BIOPOLIMEROS MEDIANTE LA MANIPULACION DE LA GENETICA Y LA ENZIMOLOGIA DE LA SINTESIS DE POLIESTERES DE POLIHIDROXIBUTIRATO (PHB) Y POLIHIDROXIALCANOATO (PHA) A NIVEL MOLECULAR EN CELULAS PROCARIOTAS Y EUCARIOTAS, EN ESPECIAL PLANTAS. LOS EJEMPLOS DEMUESTRAN EL AISLAMIENTO, LA CARACTERIZACION Y LA EXPRESION DE LOS GENES IMPLICADOS EN LA PRODUCCION DE LOS POLIMEROS DE PHB Y PHA. SE IDENTIFICARON O AISLARON LOS GENES QUE CODIFICAN LAS ENZIMAS EN LA VIA SINTETICA DE PHB Y PHA (BETA CETOTIOLASA, ACETOACETIL - COA REDUCTASA Y PHB POLIMERASA O PHA POLIMERASA) PROCEDENTES DE LA CEPA I - 16 M DE ZOOGLOEA RAMIGERA, ALCALIGENES EUTROPHUS, NOCARDIA SALMONICOLUR Y PSEUDOMONAS OLEOVARANS, Y SE EXPRESARON EN UN ORGANISMO NO PRODUCTOR DE PHB, E. COLI. LA MODIFICACION ESPECIFICA DE LOS POLIMEROS INCLUYE LA VARIACION EN LA LONGITUD DE LA CADENA DE LOS POLIMEROS, Y LA INCORPORACION DE DIFERENTES MONOMEROS EN LOS POLIMEROS PARA PRODUCIR CO- POLIMEROS CON DIFERENTES PROPIEDADES FISICAS.
ES97203215T 1989-07-10 1990-07-10 Una planta o una celula vegetal recombinante capaz de producir polihidroxibutirato u otro polihidroxialcanoato. Expired - Lifetime ES2131489T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37815589A 1989-07-10 1989-07-10

Publications (2)

Publication Number Publication Date
ES2131489T1 true ES2131489T1 (es) 1999-08-01
ES2131489T3 ES2131489T3 (es) 2003-05-16

Family

ID=23491962

Family Applications (2)

Application Number Title Priority Date Filing Date
ES90911355T Expired - Lifetime ES2125222T3 (es) 1989-07-10 1990-07-10 Procedimiento de produccion de biopolimeros a base de poliesteres.
ES97203215T Expired - Lifetime ES2131489T3 (es) 1989-07-10 1990-07-10 Una planta o una celula vegetal recombinante capaz de producir polihidroxibutirato u otro polihidroxialcanoato.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES90911355T Expired - Lifetime ES2125222T3 (es) 1989-07-10 1990-07-10 Procedimiento de produccion de biopolimeros a base de poliesteres.

Country Status (8)

Country Link
EP (2) EP0870837B1 (es)
JP (8) JPH05500751A (es)
AT (2) ATE227340T1 (es)
CA (1) CA2062816C (es)
DE (3) DE870837T1 (es)
DK (1) DK0482077T3 (es)
ES (2) ES2125222T3 (es)
WO (1) WO1991000917A1 (es)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480794A (en) * 1987-06-29 1996-01-02 Massachusetts Institute Of Technology And Metabolix, Inc. Overproduction and purification of soluble PHA synthase
GB9108756D0 (en) 1991-04-24 1991-06-12 Ici Plc Production of polyalkanoate in plants
GB9115245D0 (en) 1991-07-16 1991-08-28 Ici Plc Production of polyalkanoate
ATE295891T1 (de) * 1991-07-19 2005-06-15 Univ Michigan State Transgene pflanzen die polyhydroxyalkanoate produzieren
US5610041A (en) * 1991-07-19 1997-03-11 Board Of Trustees Operating Michigan State University Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
US5569595A (en) * 1991-09-27 1996-10-29 Center For Innovative Technology Production of poly-β-hydroxybutyrate in prokaryotic host cells
GB9223332D0 (en) * 1992-11-06 1992-12-23 Ici Plc Production of polyhydroxyalkanoate in plants
WO1994012014A1 (en) * 1992-11-20 1994-06-09 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
WO1995021257A1 (en) * 1994-02-03 1995-08-10 Center For Innovative Technology Improved production of poly-beta-hydroxyalkanoates in transformed prokaryotic hosts
CA2191713C (en) * 1994-06-01 2000-08-29 Isao Noda Process for recovering polyhydroxyalkanoates using centrifugal fractionation
CN1079835C (zh) * 1994-06-01 2002-02-27 普罗克特和甘保尔公司 利用空气分级作用回收聚羟基链烷酸酯的方法
US6083729A (en) * 1995-10-26 2000-07-04 Metabolix, Inc. Methods for isolating polyhydroxyalkanoates from plants
EP0870053A4 (en) * 1995-12-19 2002-09-11 Univ Minnesota METABOLIC PROCESS FOR THE MANUFACTURE OF POLYHYDROXYALCANOATE MONOMER SYNTHASES
GB9605704D0 (en) * 1996-03-19 1996-05-22 Zeneca Ltd Microorganisms and processes using them
WO1997038110A2 (en) * 1996-04-05 1997-10-16 Kieta Holding S.A. Compositions and methods relating to drug discovery and detection and treatment of gastrointestinal diseases
US5811272A (en) * 1996-07-26 1998-09-22 Massachusetts Institute Of Technology Method for controlling molecular weight of polyhydroxyalkanoates
JP3062459B2 (ja) * 1996-08-14 2000-07-10 理化学研究所 ポリエステル重合酵素遺伝子及びポリエステルの製造方法
US6117658A (en) * 1997-02-13 2000-09-12 James Madison University Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units
US6391611B1 (en) 1997-04-01 2002-05-21 Japan Science And Technology Corporation Polyester synthase and a gene coding for the same
JPH10276781A (ja) * 1997-04-01 1998-10-20 Rikagaku Kenkyusho ポリエステル重合酵素及び該酵素をコードする遺伝子
EP0881293A1 (en) * 1997-05-28 1998-12-02 Eidgenössische Technische Hochschule Zürich Institut für Biotechnologie Production of medium chain length poly-3-hydroxy alkanoates in Escherichia coli, and monomers derived therefrom
DE69838768T2 (de) 1997-09-19 2008-10-30 Metabolix, Inc., Cambridge Biologische Systeme zur Herstellung von Polyhydroxyalkanoat-Polymeren die 4-Hy droxysäure enthalten
AU5907198A (en) * 1998-01-05 1999-07-26 Monsanto Company Biosynthesis of medium chain length polyhydroxyalkanoates
US6207217B1 (en) 1998-01-07 2001-03-27 Pioneer Hi-Bred International, Inc. Animal nutrition compositions
US6586658B1 (en) 1998-03-06 2003-07-01 Metabolix, Inc. Modification of fatty acid metabolism in plants
US6143952A (en) * 1998-03-31 2000-11-07 Regents Of The University Of Minnesota Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
US6103956A (en) * 1998-03-31 2000-08-15 Regents Of The University Of Minnesota Polyhydroxyalkanoate synthesis in plants
ATE458822T1 (de) 1998-04-08 2010-03-15 Metabolix Inc Verfahren für die trennung und reinigung von biopolymeren
AU4199599A (en) 1998-05-22 1999-12-13 Metabolix, Inc. Polyhydroxyalkanoate biopolymer compositions
MXPA01001049A (es) * 1998-07-30 2002-08-20 Metabolix Inc Enzimas para produccion de biopolimeros.
AU5247999A (en) * 1998-07-30 2000-02-21 Metabolix, Inc. Production of block copolymers of polyhydroxyalkanoates in biological systems
ES2302386T3 (es) * 1998-08-04 2008-07-01 Metabolix, Inc. Produccion de polihidroxialcanoatos a partir de polioles.
ATE319835T1 (de) * 1998-08-18 2006-03-15 Metabolix Inc Transgene polyhydroxyalkanoat produzierende mikroben
WO2000052183A1 (en) * 1999-03-05 2000-09-08 Monsanto Technology Llc Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways
AU2001236839A1 (en) 2000-02-11 2001-08-20 Metabolix, Inc. Multi-gene expression constructs containing modified inteins
US6808910B2 (en) 2000-03-30 2004-10-26 Canon Kabushiki Kaisha Polyhydroxyalkanoate synthase and gene encoding the same enzyme
JP3848046B2 (ja) * 2000-03-30 2006-11-22 キヤノン株式会社 ポリヒドロキシアルカノエート合成酵素及び該酵素をコードする遺伝子
US6803220B2 (en) 2000-03-30 2004-10-12 Canon Kabushiki Kaisha Polyhydroxyalkanoate synthase and gene encoding the same enzyme
US6812013B2 (en) 2000-03-30 2004-11-02 Canon Kabushiki Kaisha Polyhydroxyalkanoate synthase and gene encoding the same
US6875596B2 (en) 2000-03-30 2005-04-05 Canon Kabushiki Kaisha Polyhydroxyalkanoate synthase and gene encoding the same enzyme
JP3848047B2 (ja) 2000-03-30 2006-11-22 キヤノン株式会社 ポリヒドロキシアルカノエート合成酵素及び該酵素をコードする遺伝子
JP3848048B2 (ja) * 2000-03-30 2006-11-22 キヤノン株式会社 ポリヒドロキシアルカノエート合成酵素及び該酵素をコードする遺伝子
US6803219B2 (en) 2000-03-30 2004-10-12 Canon Kabushiki Kaisha Polyhydroxyalkanoate synthase and gene encoding the same enzyme
WO2002040690A2 (en) 2000-11-17 2002-05-23 Metabolix, Inc. Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
US6808907B2 (en) * 2001-03-27 2004-10-26 Canon Kabushiki Kaisha Method and apparatus for producing polyhydroxyalkanoate
CA2800359A1 (en) 2005-03-16 2006-09-28 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways
CN105936887A (zh) 2007-03-16 2016-09-14 基因组股份公司 用于1,4-丁二醇和其前体生物合成的组合物和方法
JP5912529B2 (ja) 2008-09-10 2016-04-27 ゲノマチカ， インク． １，４−ブタンジオールの生成のための微生物体
CA2754108A1 (en) 2009-03-05 2010-09-10 Metabolix, Inc. Propagation of transgenic plants
WO2010102217A1 (en) 2009-03-05 2010-09-10 Metabolix, Inc. Stable, fertile, high polyhydroxyalkanoate producing plants and methods of producing them
CN102498215A (zh) 2009-06-04 2012-06-13 基因组股份公司 生产1,4-丁二醇的微生物和相关方法
US20120174253A1 (en) 2009-09-15 2012-07-05 Nii Patterson Generation of high polyhydroxybutrate producing oilseeds
US20120276606A1 (en) 2009-10-30 2012-11-01 Daicel Corporation Recombinant microorganisms with 1,3-butanediol-producing function and uses thereof
US8530210B2 (en) 2009-11-25 2013-09-10 Genomatica, Inc. Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone
EP2534141B1 (en) 2010-02-11 2016-04-20 Metabolix, Inc. Process for gamma-butyrolactone production
KR102005592B1 (ko) 2011-10-25 2019-07-30 마론 바이오 이노베이션스, 인코포레이티드 크로모박테리움 제제, 조성물, 대사물 및 이들의 용도
EP2855687B1 (en) 2012-06-04 2020-04-22 Genomatica, Inc. Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds
IT201800003441A1 (it) * 2018-03-12 2019-09-12 Bio On Spa Poliidrossialcanoati per l’uso nella prevenzione del cancro colorettale.
CN110699391B (zh) * 2019-11-01 2023-05-30 山东省农业科学院农产品研究所 一种微波辅助碱法预处理牡丹果荚制备生物塑料聚-β-羟丁酸的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE212052T1 (de) * 1987-06-29 2002-02-15 Massachusetts Inst Technology Nucleotidsequenz für acetoacetyl-coa reduktase kodierend und verfahren zur herstellung von polyester-biopolymeren

Also Published As

Publication number Publication date
WO1991000917A1 (en) 1991-01-24
DK0482077T3 (da) 1999-06-28
CA2062816A1 (en) 1991-01-11
JP2005278656A (ja) 2005-10-13
JP2004329219A (ja) 2004-11-25
JP2003189858A (ja) 2003-07-08
DE69032713T2 (de) 1999-04-08
ES2131489T3 (es) 2003-05-16
EP0482077A1 (en) 1992-04-29
JP2005287517A (ja) 2005-10-20
EP0482077B1 (en) 1998-10-21
JP2007143567A (ja) 2007-06-14
ATE227340T1 (de) 2002-11-15
CA2062816C (en) 2006-09-12
DE69034017T2 (de) 2003-07-31
ATE172497T1 (de) 1998-11-15
DE870837T1 (de) 1999-09-16
JP3964436B2 (ja) 2007-08-22
ES2125222T3 (es) 1999-03-01
EP0870837B1 (en) 2002-11-06
JP3703983B2 (ja) 2005-10-05
DE69034017D1 (de) 2002-12-12
JPH05500751A (ja) 1993-02-18
JP2001178482A (ja) 2001-07-03
JP3964437B2 (ja) 2007-08-22
JPH11332588A (ja) 1999-12-07
EP0870837A1 (en) 1998-10-14
DE69032713D1 (de) 1998-11-26
JP3967371B2 (ja) 2007-08-29

Similar Documents

Publication Publication Date Title
ES2131489T1 (es) Metodo para la produccion de nuevos biopolimeros de poliester.
Tokiwa et al. Biotechnological production of (R)-3-hydroxybutyric acid monomer
Kulkarni et al. Production and characterization of a biodegradable poly (hydroxybutyrate-co-hydroxyvalerate)(PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake, India
Akiyama et al. Production of poly (3-hydroxyalkanoates) by a bacterium of the genus Alcaligenes utilizing long-chain fatty acids
Gorenflo et al. Development of a process for the biotechnological large-scale production of 4-hydroxyvalerate-containing polyesters and characterization of their physical and mechanical properties
Thakor et al. Biosynthesis of medium chain length poly (3-hydroxyalkanoates)(mcl-PHAs) by Comamonas testosteroni during cultivation on vegetable oils
Savenkova et al. Effect of 3-hydroxy valerate content on some physical and mechanical properties of polyhydroxyalkanoates produced by Azotobacter chroococcum
WO1999061624A2 (en) Polyhydroxyalkanoate biopolymer compositions
Chanprateep et al. Production and characterization of biodegradable terpolymer poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) by Alcaligenes sp. A-04
CN102066560B (zh) 使用重组微生物制造共聚聚酯的方法
DE69232694D1 (de) Herstellung von polyhydroxyalkanoate in pflanzen
KR100462543B1 (ko) 폴리하이드록시알카노에이트 및 그 제조방법
KR100467208B1 (ko) 원료로서의 치환된 지방산 에스테르로부터의폴리하이드록시알카노에이트의 제조방법
Lakshmanan et al. Biosynthesis and characterization of co and ter-polyesters of polyhydroxyalkanoates containing high monomeric fractions of 4-hydroxybutyrate and 5-hydroxyvalerate via a novel PHA synthase
Muzaiyanah et al. Studies on the microbial synthesis and characterization of polyhydroxyalkanoates containing 4-hydroxyvalerate using γ-valerolactone
US20140378646A1 (en) UTILIZATION OF THE NOVEL, ENVIRONMENTAL ISOLATES PSEUDOMONAS sp. IPB-B26 AND N-128 FOR THE EFFICIENT HIGH YIELD PRODUCTION OF mcl/lcl-PHAs
WO1989000202A3 (en) Method for producing novel polyester biopolymers
Takeda et al. Biosynthesis of poly-3-hydroxybutyrate by Sphaerotilus natans
Ramachander et al. Synthesis of PHB by recombinant E. coli harboring an approximately 5 kb genomic DNA fragment from Streptomyces aureofaciens NRRL 2209
Kim et al. Characterization of an extracellular poly (3-hydroxy-5-phenylvalerate) depolymerase from Xanthomonas sp. JS02
US20160040197A1 (en) PRODUCTION METHOD FOR COPOLYMER POLYHYDROXYALKANOATE USING GENETICALLY MODIFIED STRAIN OF FATTY ACID ß-OXIDATION PATHWAY
Kamachi et al. Enzymatic polymerization and characterization of new poly (3-hydroxyalkanoate) s by a bacterial polymerase
JP5555975B2 (ja) ポリヒドロキシアルカノエートの製造方法
CHAI et al. Isolation and identification of P (3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
Mohapatra et al. Polyhydroxyalkanoates: the green polymer