ECSP024291A - Procedimiento para la sintesis rapida de peptidos en solucion - Google Patents
Procedimiento para la sintesis rapida de peptidos en solucionInfo
- Publication number
- ECSP024291A ECSP024291A EC2002004291A ECSP024291A ECSP024291A EC SP024291 A ECSP024291 A EC SP024291A EC 2002004291 A EC2002004291 A EC 2002004291A EC SP024291 A ECSP024291 A EC SP024291A EC SP024291 A ECSP024291 A EC SP024291A
- Authority
- EC
- Ecuador
- Prior art keywords
- peptide
- stage
- activated carboxylic
- solution
- sweeping agent
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 230000015572 biosynthetic process Effects 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 2
- 238000003786 synthesis reaction Methods 0.000 title abstract 2
- 238000010408 sweeping Methods 0.000 abstract 3
- 150000001450 anions Chemical class 0.000 abstract 2
- 238000010511 deprotection reaction Methods 0.000 abstract 2
- 238000000605 extraction Methods 0.000 abstract 2
- 150000001412 amines Chemical class 0.000 abstract 1
- 230000008033 biological extinction Effects 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 150000003573 thiols Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un procedimiento para la síntesis rápida de un péptido en solución, que comprende ciclos repetidos de las etapas (a)-(d): (a) una etapa de acoplamiento, usando un exceso de un componente carboxílico activado para acilar un componente amino, (b) una etapa de extinción en que se usa un agente barredor para remover las funciones carboxíclicas activadas residuales, donde el agente barredor también puede usarse para la desprotección del péptido en crecimiento, (c) una o más extracciones acuosas y opcionalmente, (d) una etapa de desprotección separada, seguida por una o más extracciones acuosas, el procedimiento caracterizado porque comprende al menos una etapa (b), denominada etapa (b´) en que una amina o un tiol que comprende un anión libre o un anión latente se usa como agente barredor de las funciones carboxílicas activadas residuales. Durante el procedimiento de esta invención, el péptido en crecimiento no necesita ser aislado que se haya obtenido la secuencia final del péptido. Este procedimiento altamente eficiente es útil para la producción de oligo- y polipéptidos de alta pureza.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01202753 | 2001-07-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP024291A true ECSP024291A (es) | 2002-09-27 |
Family
ID=8180656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EC2002004291A ECSP024291A (es) | 2001-07-19 | 2002-07-19 | Procedimiento para la sintesis rapida de peptidos en solucion |
Country Status (25)
| Country | Link |
|---|---|
| US (4) | US20030018164A1 (es) |
| EP (1) | EP1291356B1 (es) |
| JP (1) | JP4142907B2 (es) |
| KR (1) | KR100861031B1 (es) |
| CN (1) | CN1254483C (es) |
| AR (1) | AR034869A1 (es) |
| AT (1) | ATE302792T1 (es) |
| BR (1) | BRPI0202783B1 (es) |
| CA (1) | CA2390358C (es) |
| DE (1) | DE60205693T2 (es) |
| DK (1) | DK1291356T3 (es) |
| EC (1) | ECSP024291A (es) |
| ES (1) | ES2248485T3 (es) |
| HR (1) | HRP20020609B1 (es) |
| HU (1) | HUP0202369A2 (es) |
| IL (1) | IL150601A (es) |
| MX (1) | MXPA02006998A (es) |
| NO (1) | NO324245B1 (es) |
| PE (1) | PE20030214A1 (es) |
| PL (1) | PL205559B1 (es) |
| PT (1) | PT1291356E (es) |
| RU (1) | RU2237673C2 (es) |
| SG (1) | SG119160A1 (es) |
| TW (1) | TWI247012B (es) |
| ZA (1) | ZA200205409B (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL150601A (en) * | 2001-07-19 | 2010-06-30 | Organon Nv | Process for rapid synthesis of peptides within a solution using the back of an activated carboxyl component and destruction of the residual activated carboxyl component |
| EP1790656A1 (en) * | 2005-11-25 | 2007-05-30 | Nanokem S.A. | Solution-phase synthesis of leuprolide |
| EP1801086A1 (en) * | 2005-11-25 | 2007-06-27 | Synthacon GmbH | Synthesis of carbon acid amides |
| BRPI0706400A2 (pt) * | 2006-01-17 | 2011-03-29 | Organon Nv | processos para a hidrólise enzimática seletiva de terc-butil ésteres c-terminais de substratos de peptìdeo, para a sìntese convergente de um peptìdeo de dois ou mais fragmentos de peptìdeo, para a sìntese enzimática por etapas de um peptìdeo na direção do terminal c, e para a sìntese de peptìdeo |
| US8716439B2 (en) | 2006-03-01 | 2014-05-06 | Kaneka Corporation | Method of producing peptide |
| US8124372B2 (en) | 2007-06-25 | 2012-02-28 | N.V. Organon | Selective enzymatic amidation of C-terminal esters or acids of peptides |
| DE602008004350D1 (de) * | 2007-06-25 | 2011-02-17 | Organon Nv | Verfahren zur umsetzung von c-terminalen peptidestern oder -säuren zu amiden unter verwendung von subtilisin in gegenwart von ammoniumsalzen |
| US9850274B2 (en) * | 2008-12-30 | 2017-12-26 | Gkl-Biotec Ag | Peptide combination |
| KR101032399B1 (ko) * | 2010-02-11 | 2011-05-03 | 주식회사 이너트론 | 밴드패스필터의 철재하우징 및 그 제조방법 |
| EP2409982A3 (en) * | 2010-02-25 | 2012-02-08 | Corning Incorporated | Chemical processes generating solid(s) carried out continuously within microreactors |
| JP6136934B2 (ja) | 2011-12-15 | 2017-05-31 | 味の素株式会社 | Ｆｍｏｃ基の除去方法 |
| JP6713983B2 (ja) | 2015-03-04 | 2020-06-24 | Jitsubo株式会社 | ペプチド合成方法 |
| KR102479601B1 (ko) | 2015-12-21 | 2022-12-22 | 텍사스 테크 유니버시티 시스템 | 용액상 gap 펩타이드 합성을 위한 시스템 및 방법 |
| DK3778621T5 (da) | 2018-04-13 | 2024-08-19 | Jitsubo Co Ltd | Peptidsyntesefremgangsmåde |
| US12024537B2 (en) | 2018-05-31 | 2024-07-02 | Sederma | Compositions and methods for chemical synthesis |
| FR3090636B1 (fr) * | 2018-12-24 | 2021-01-01 | Strainchem | Procédé de synthèse de peptides |
| CA3126705A1 (en) | 2019-02-01 | 2020-08-06 | Gap Peptides Llc | Synthesis strategy for gap protecting group |
| JP7505498B2 (ja) * | 2019-08-30 | 2024-06-25 | 日産化学株式会社 | ペプチド化合物の製造方法 |
| JP7709920B2 (ja) | 2019-12-27 | 2025-07-17 | 中外製薬株式会社 | ペプチド化合物の合成法 |
| CN115362144A (zh) * | 2020-03-27 | 2022-11-18 | 株式会社钟化 | 含酰胺键化合物的制造方法 |
| JP7063408B1 (ja) | 2021-07-02 | 2022-05-09 | ペプチスター株式会社 | 液相ペプチド製造方法 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1991A (en) * | 1841-02-23 | Manner of fastening bedsteads | ||
| US5221754A (en) * | 1989-06-09 | 1993-06-22 | Research Corporation Technologies, Inc. | Reagents for rapid peptide synthesis |
| US5101059A (en) * | 1989-12-05 | 1992-03-31 | Research Corporation Technologies, Inc. | Amino acid protecting groups |
| US5877278A (en) | 1992-09-24 | 1999-03-02 | Chiron Corporation | Synthesis of N-substituted oligomers |
| US5516892A (en) | 1992-12-28 | 1996-05-14 | Indiana University Foundation | Polymer-bound mixed carboxylic anhdrides as a stable form of activated carboxylic acids |
| US6310180B1 (en) * | 1993-06-21 | 2001-10-30 | Vanderbilt University | Method for synthesis of proteins |
| US5516639A (en) * | 1993-07-22 | 1996-05-14 | Mayo Foundation For Medical Education And Research | Antibodies specific for human prostate glandular kallkrein |
| US6001966A (en) * | 1995-10-19 | 1999-12-14 | Proligo Llc | Method for solution phase synthesis of oligonucleotides and peptides |
| US5698676A (en) * | 1995-11-30 | 1997-12-16 | Abbott Laboratories | Use of propylene oxide as an acid scavenger in peptide synthesis |
| US5849954A (en) * | 1996-01-18 | 1998-12-15 | Research Corporation Technologies, Inc. | Method of peptide synthesis |
| AU737428B2 (en) | 1996-05-03 | 2001-08-16 | Warner-Lambert Company | Rapid purification by polymer supported quench |
| US6121488A (en) * | 1997-09-24 | 2000-09-19 | Warner-Lambert Company | Quenching reagents for solution phase synthesis |
| JPH11217397A (ja) * | 1997-11-27 | 1999-08-10 | Saburo Aimoto | ペプチドチオールエステルの製造方法 |
| FR2780061B1 (fr) | 1998-06-22 | 2001-09-07 | Rhone Poulenc Rorer Sa | Nouveau procede de preparation de derives de cyclosporine |
| CA2373816A1 (en) * | 1999-05-26 | 2000-11-30 | Abbott Laboratories | Minimal isolation peptide synthesis process using ion-exchange resins as scavenging agents |
| US20020127219A1 (en) | 1999-12-30 | 2002-09-12 | Okkels Jens Sigurd | Lysosomal enzymes and lysosomal enzyme activators |
| TW200628612A (en) | 2000-07-19 | 2006-08-16 | Pharmacia & Up John Company | Substrates and assays for β-secretase activity |
| IL150600A (en) * | 2001-07-19 | 2005-09-25 | Akzo Nobel Nv | Process for the preparation of compounds containing one or more amide bonds using an excess of an activated carboxylic component and scavenging the residual activated carboxylic component |
| IL150601A (en) * | 2001-07-19 | 2010-06-30 | Organon Nv | Process for rapid synthesis of peptides within a solution using the back of an activated carboxyl component and destruction of the residual activated carboxyl component |
| US7834129B2 (en) * | 2005-06-17 | 2010-11-16 | Eastman Chemical Company | Restaurant smallware comprising polyester compositions formed from 2,2,4,4-tetramethyl-1,3-cyclobutanediol and 1,4-cyclohexanedimethanol |
-
2002
- 2002-07-04 IL IL150601A patent/IL150601A/en active IP Right Grant
- 2002-07-04 TW TW091114832A patent/TWI247012B/zh not_active IP Right Cessation
- 2002-07-05 JP JP2002197550A patent/JP4142907B2/ja not_active Expired - Lifetime
- 2002-07-05 ZA ZA200205409A patent/ZA200205409B/xx unknown
- 2002-07-15 DE DE60205693T patent/DE60205693T2/de not_active Expired - Lifetime
- 2002-07-15 EP EP02077830A patent/EP1291356B1/en not_active Expired - Lifetime
- 2002-07-15 DK DK02077830T patent/DK1291356T3/da active
- 2002-07-15 PT PT02077830T patent/PT1291356E/pt unknown
- 2002-07-15 ES ES02077830T patent/ES2248485T3/es not_active Expired - Lifetime
- 2002-07-15 SG SG200204298A patent/SG119160A1/en unknown
- 2002-07-15 AT AT02077830T patent/ATE302792T1/de active
- 2002-07-17 PE PE2002000637A patent/PE20030214A1/es not_active Application Discontinuation
- 2002-07-17 MX MXPA02006998A patent/MXPA02006998A/es active IP Right Grant
- 2002-07-18 NO NO20023446A patent/NO324245B1/no not_active IP Right Cessation
- 2002-07-18 BR BRPI0202783A patent/BRPI0202783B1/pt active IP Right Grant
- 2002-07-18 HU HU0202369A patent/HUP0202369A2/hu unknown
- 2002-07-18 KR KR1020020041898A patent/KR100861031B1/ko not_active Expired - Fee Related
- 2002-07-18 CN CNB021261911A patent/CN1254483C/zh not_active Expired - Lifetime
- 2002-07-18 AR ARP020102687A patent/AR034869A1/es active IP Right Grant
- 2002-07-18 RU RU2002119630/04A patent/RU2237673C2/ru not_active IP Right Cessation
- 2002-07-18 CA CA002390358A patent/CA2390358C/en not_active Expired - Lifetime
- 2002-07-19 US US10/199,805 patent/US20030018164A1/en not_active Abandoned
- 2002-07-19 HR HR20020609A patent/HRP20020609B1/xx not_active IP Right Cessation
- 2002-07-19 EC EC2002004291A patent/ECSP024291A/es unknown
- 2002-07-19 PL PL355125A patent/PL205559B1/pl not_active IP Right Cessation
-
2003
- 2003-10-22 US US10/692,354 patent/US20040082760A1/en not_active Abandoned
- 2003-10-23 US US10/693,123 patent/US20060063918A1/en not_active Abandoned
- 2003-10-23 US US10/693,802 patent/US7435791B2/en not_active Expired - Lifetime
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