EA200100819A1 - Способ получения мембранных пузырьков - Google Patents
Способ получения мембранных пузырьковInfo
- Publication number
- EA200100819A1 EA200100819A1 EA200100819A EA200100819A EA200100819A1 EA 200100819 A1 EA200100819 A1 EA 200100819A1 EA 200100819 A EA200100819 A EA 200100819A EA 200100819 A EA200100819 A EA 200100819A EA 200100819 A1 EA200100819 A1 EA 200100819A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- obtaining membrane
- bubbles
- membrane bubbles
- obtaining
- vesicles
- Prior art date
Links
- 239000012528 membrane Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 210000000612 antigen-presenting cell Anatomy 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000005227 gel permeation chromatography Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 210000004881 tumor cell Anatomy 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0011—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5154—Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Oncology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
Способ получения мембранных пузырьков из биологического образца характеризуется тем, что он содержит, по меньшей мере, одну стадию обработки образца путем анионообменной и/или гельпроникающей хроматографии. Изобретение используют для получения пузырьков различного происхождения и различной природы, в частности из антигенпредставляющих клеток или опухолевых клеток. Изобретение относится также к таким образом полученным пузырькам и их применениям.Отчет о международном поиске был опубликован 2000.11.16.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9900886A FR2788780B1 (fr) | 1999-01-27 | 1999-01-27 | Procede de preparation de vesicules membranaires |
PCT/FR2000/000105 WO2000044389A2 (fr) | 1999-01-27 | 2000-01-19 | Procede de preparation de vesicules membranaires |
Publications (2)
Publication Number | Publication Date |
---|---|
EA200100819A1 true EA200100819A1 (ru) | 2001-12-24 |
EA003235B1 EA003235B1 (ru) | 2003-02-27 |
Family
ID=9541269
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA200100819A EA003235B1 (ru) | 1999-01-27 | 2000-01-19 | Способ получения мембранных пузырьков |
Country Status (11)
Country | Link |
---|---|
US (1) | US6899863B1 (ru) |
EP (1) | EP1143982A2 (ru) |
JP (1) | JP2002535665A (ru) |
CN (1) | CN1355704A (ru) |
AU (1) | AU768322B2 (ru) |
CA (1) | CA2360752A1 (ru) |
EA (1) | EA003235B1 (ru) |
FR (1) | FR2788780B1 (ru) |
HK (1) | HK1042647A1 (ru) |
IL (1) | IL143692A0 (ru) |
WO (1) | WO2000044389A2 (ru) |
Families Citing this family (108)
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CN109825472A (zh) * | 2019-03-01 | 2019-05-31 | 易春 | 一种细胞外囊泡的提取方法及试剂盒 |
JP7513869B2 (ja) | 2020-03-23 | 2024-07-10 | 澁谷工業株式会社 | エクソソーム抽出装置およびエクソソームの抽出方法 |
WO2021209995A1 (en) | 2020-04-13 | 2021-10-21 | Exoprother Medical Ltd. | Cell-derived vesicles comprising wild-type p53 protein for antiviral therapy |
US20210322483A1 (en) | 2020-04-16 | 2021-10-21 | Ichilov Tech Ltd. | Cell-derived particles presenting heterologous cd24 and use thereof in therapy |
GB202010278D0 (en) | 2020-07-03 | 2020-08-19 | Evox Therapeutics Ltd | Extracellular vesicles with improved half-life |
IL277743A (en) | 2020-10-01 | 2022-04-01 | Yeda Res & Dev | A method for diagnosing breast cancer |
WO2022146083A1 (ko) * | 2020-12-30 | 2022-07-07 | 한국과학기술원 | 엑소좀 분리용 조성물 및 이를 이용한 엑소좀 분리 방법 |
GB202105925D0 (en) | 2021-04-26 | 2021-06-09 | Evox Therapeutics Ltd | Modified extracellular vesicles (EVs) with improved half-life |
JP2023104650A (ja) * | 2022-01-18 | 2023-07-28 | ダイセン・メンブレン・システムズ株式会社 | 細胞外小胞の分離精製方法 |
WO2024024336A1 (ja) * | 2022-07-28 | 2024-02-01 | 株式会社ダイセル | 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質の精製濃縮液の製造方法 |
CN117070450B (zh) * | 2023-10-12 | 2024-01-16 | 哈药集团生物工程有限公司 | 一种外泌体的纯化方法 |
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DE3852037T2 (de) * | 1987-07-29 | 1995-03-23 | Liposome Co Inc | Verfahren zur trennung von teilchen nach grösse. |
ATE319477T1 (de) * | 1995-08-03 | 2006-03-15 | Univ Leiden | Antigen presentierende bläschen, die von zellen ableiten |
US6165785A (en) * | 1996-05-24 | 2000-12-26 | University Of Cincinnati | Bone marrow cultures for developing suppressor and stimulator cells for research and therapeutic applications |
AU2710897A (en) * | 1996-05-31 | 1998-01-05 | Whitaker Corporation, The | Rechargeable battery connector |
FR2766205B1 (fr) * | 1997-07-16 | 2002-08-30 | Inst Nat Sante Rech Med | Nouveau procede de sensibilisation de cellules presentatrices d'antigene et nouveaux moyens pour la mise en oeuvre du procede |
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1999
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2000
- 2000-01-19 CN CN00802903A patent/CN1355704A/zh active Pending
- 2000-01-19 EA EA200100819A patent/EA003235B1/ru not_active IP Right Cessation
- 2000-01-19 WO PCT/FR2000/000105 patent/WO2000044389A2/fr not_active Application Discontinuation
- 2000-01-19 US US09/890,319 patent/US6899863B1/en not_active Expired - Fee Related
- 2000-01-19 CA CA002360752A patent/CA2360752A1/fr not_active Abandoned
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- 2000-01-19 EP EP00900609A patent/EP1143982A2/fr not_active Withdrawn
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WO2000044389A3 (fr) | 2000-11-16 |
EA003235B1 (ru) | 2003-02-27 |
JP2002535665A (ja) | 2002-10-22 |
WO2000044389A2 (fr) | 2000-08-03 |
EP1143982A2 (fr) | 2001-10-17 |
FR2788780A1 (fr) | 2000-07-28 |
CA2360752A1 (fr) | 2000-08-03 |
IL143692A0 (en) | 2002-04-21 |
AU3056100A (en) | 2000-08-18 |
HK1042647A1 (zh) | 2002-08-23 |
AU768322B2 (en) | 2003-12-11 |
FR2788780B1 (fr) | 2001-03-30 |
US6899863B1 (en) | 2005-05-31 |
CN1355704A (zh) | 2002-06-26 |
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