ATE403671T1 - Methoden zur aufreinigung von rekombinatem humanem erythropoietin aus zellkulturüberständen - Google Patents

Methoden zur aufreinigung von rekombinatem humanem erythropoietin aus zellkulturüberständen

Info

Publication number
ATE403671T1
ATE403671T1 AT99958811T AT99958811T ATE403671T1 AT E403671 T1 ATE403671 T1 AT E403671T1 AT 99958811 T AT99958811 T AT 99958811T AT 99958811 T AT99958811 T AT 99958811T AT E403671 T1 ATE403671 T1 AT E403671T1
Authority
AT
Austria
Prior art keywords
epo
human erythropoietin
supernatives
purification
methods
Prior art date
Application number
AT99958811T
Other languages
English (en)
Inventor
Carlos Miguel Carcagno
Marcelo Criscuolo
Carlos Melo
Juan Alejandro Vidal
Original Assignee
Sterrenbeld Biotechnologie North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from ARP980105610 external-priority patent/AR008359A1/es
Priority claimed from ARP990100680 external-priority patent/AR008364A1/es
Application filed by Sterrenbeld Biotechnologie North America Inc filed Critical Sterrenbeld Biotechnologie North America Inc
Application granted granted Critical
Publication of ATE403671T1 publication Critical patent/ATE403671T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99958811T 1998-11-06 1999-11-08 Methoden zur aufreinigung von rekombinatem humanem erythropoietin aus zellkulturüberständen ATE403671T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ARP980105610 AR008359A1 (es) 1998-11-06 1998-11-06 PROCEDIMIENTO PARA LA PURIFICACIoN DE ERITROPOYETINA HUMANA RECOMBINANTE A PARTIR DE SOBRENADANTES DE CULTIVO DE CÉLULAS.
ARP990100680 AR008364A1 (es) 1999-02-23 1999-02-23 Procedimiento para la purificacion de eritropoyetina humana recombinante a partir de sobrenadantes de cultivo de celulas y eritropoyetina humanarecombinante obtenida con tal procedimiento

Publications (1)

Publication Number Publication Date
ATE403671T1 true ATE403671T1 (de) 2008-08-15

Family

ID=25590827

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99958811T ATE403671T1 (de) 1998-11-06 1999-11-08 Methoden zur aufreinigung von rekombinatem humanem erythropoietin aus zellkulturüberständen

Country Status (11)

Country Link
US (1) US7012130B1 (de)
EP (1) EP1127063B1 (de)
JP (1) JP4516692B2 (de)
AT (1) ATE403671T1 (de)
AU (1) AU1610000A (de)
BR (1) BR9917606A (de)
CA (2) CA2704598A1 (de)
CO (1) CO5241324A1 (de)
DE (1) DE69939268D1 (de)
ES (1) ES2312219T3 (de)
WO (1) WO2000027869A1 (de)

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US6777205B1 (en) 1998-11-06 2004-08-17 Sterrenbeld Biotechnologie North America, Inc. Host cells expressing recombinant human erythropoietin
BR9917606A (pt) 1998-11-06 2002-12-31 Bio Sidus S A Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento
BR9905868A (pt) 1998-11-06 2001-01-23 Bio Sidus S A Procedimento de cultivo massivo de células de mamìfero para a obtenção de eritropoetina humana, recombinante e a eritropoetina humana recombinante obtida com tal procedimento
PL368755A1 (en) 2001-11-28 2005-04-04 Sandoz Ag Chromatographic purification of recombinant human erythropoietin
KR100567448B1 (ko) * 2003-11-05 2006-04-04 주식회사 인투젠 형질전환 동물의 유즙 전처리방법 및 이를 통한 인체단백질의 정제방법
DE102004027816A1 (de) * 2004-06-08 2006-01-05 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von Erythropoietin
AR053416A1 (es) * 2005-11-10 2007-05-09 Protech Pharma S A Combinacion de glicoisoformas para el tratamiento o prevencion de la septicemia, linea celular transgenica productora de glicoformas de eritropoyetina, composicion farmaceutica que comprende a dicha combinacion, procedimientos para obtener la linea celular, procedimiento para producir dicha combinac
EP2120998B1 (de) 2006-11-28 2013-08-07 HanAll Biopharma Co., Ltd. Modifizierte erythropoetin-polypeptide und ihre verwendungen zur behandlung
KR100988706B1 (ko) 2007-03-07 2010-10-18 한미홀딩스 주식회사 재조합 에리트로포이에틴의 신규한 정제방법
CL2008002053A1 (es) 2007-07-17 2009-05-22 Hoffmann La Roche Metodo para la purificacion de una eritropoyetina monopeguilada (epompeg) que consiste en proporcionar una solucion que contiene eritropoyetina mono, poli y no peguilada y hacerla pasar por dos pasos de cromatografia de intercambio cationico y metodo para producir epo mpeg que incluye metodo de purificacion.
CL2008002054A1 (es) * 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
DE102008002209A1 (de) 2008-06-04 2009-12-10 Evonik Degussa Gmbh Verfahren zur Aufreinigung von Erythropoietin
DE102008002210A1 (de) * 2008-06-04 2009-12-10 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Erythropoietin
JP5380743B2 (ja) * 2008-06-19 2014-01-08 住友化学株式会社 光学活性な４−アミノ−３−置換フェニルブタン酸の製造方法
RU2011102447A (ru) 2008-06-24 2012-07-27 Др.Реддис' Лабораторис Лтд. (In) Очистка модифицированных цитокинов
CA2736141C (en) 2008-09-23 2018-03-20 F. Hoffmann-La Roche Ag Purification of erythropoietin
DE102008054716A1 (de) * 2008-12-16 2010-06-17 Evonik Degussa Gmbh Inprozesskontrolle in einem Verfahren zur Herstellung von EPO
KR20120117728A (ko) * 2009-09-23 2012-10-24 바이오제너릭스 에이지 재조합 인간 에리트로포이에틴(ｅｐｏ)의 정제 방법, 이렇게 정제된 ｅｐｏ 및 이를 포함하는 약학적 조성물
KR101443257B1 (ko) 2011-10-18 2014-09-19 주식회사 종근당 낮은 등전점을 갖는 에리스로포이에틴 유사체의 정제방법
WO2013153497A1 (en) 2012-04-10 2013-10-17 Dr. Reddy's Laboratories Limited Single step fractionation method
KR101414897B1 (ko) 2013-11-29 2014-07-04 씨제이헬스케어 주식회사 다베포에틴 알파의 정제 방법
JP6845139B2 (ja) 2014-12-15 2021-03-17 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ 粗製溶液からの標的分子捕捉
US10562951B2 (en) 2015-03-10 2020-02-18 Merck Sharp & Dohme Corp. Process for preparing recombinant insulin using microfiltration
EP3428284B1 (de) * 2016-03-09 2022-05-11 JCR Pharmaceuticals CO., LTD. Verfahren zur herstellung von mutiertem menschlichem erythropoietin
WO2017169978A1 (ja) * 2016-03-31 2017-10-05 株式会社カネカ 精製されたネコ由来エリスロポエチンの製造方法

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US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
KR850004274A (ko) 1983-12-13 1985-07-11 원본미기재 에리트로포이에틴의 제조방법
JPS6197229A (ja) 1984-10-18 1986-05-15 Chugai Pharmaceut Co Ltd 安定なエリトロポエチン製剤
IL77081A (en) 1984-12-04 1999-10-28 Genetics Inst AND sequence encoding human erythropoietin, a process for its preparation and a pharmacological preparation of human erythropoietin
US4667016A (en) * 1985-06-20 1987-05-19 Kirin-Amgen, Inc. Erythropoietin purification
IL79176A (en) * 1985-06-20 1992-06-21 Kirin Amgen Inc Process for the recovery of erythropoietin from a fluid
DK173067B1 (da) 1986-06-27 1999-12-13 Univ Washington Humant erythropoietin-gen, fremgangsmåde til ekspression deraf i transficerede cellelinier, de transficerede cellelinier sa
DE4014654A1 (de) * 1990-05-08 1991-11-14 Behringwerke Ag Galenische waessrige formulierungen von erythropoietin und ihre verwendung
EP0503050A4 (en) * 1990-09-28 1994-07-06 Ortho Pharma Corp Hybrid growth factors
SE9300105D0 (sv) 1993-01-15 1993-01-15 Kabi Pharmacia Ab Stable protein solution
IL118201A (en) * 1995-05-11 2004-12-15 Roche Diagnostics Gmbh Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof
US5876992A (en) 1996-07-03 1999-03-02 Molecular Biology Resources, Inc. Method and formulation for stabilization of enzymes
BR9917606A (pt) 1998-11-06 2002-12-31 Bio Sidus S A Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento
UY25790A1 (es) 1998-11-06 2000-08-21 Bio Sidus S A Formas farmaceuticas de eritropoyetina humana recombinante estables a temperatura ambiente y apropiadas para su uso en humanos, formulaciones y procedimientos de liofilizacion para su obtencion.
BR9905868A (pt) 1998-11-06 2001-01-23 Bio Sidus S A Procedimento de cultivo massivo de células de mamìfero para a obtenção de eritropoetina humana, recombinante e a eritropoetina humana recombinante obtida com tal procedimento
BR9905867A (pt) 1998-11-06 2001-01-23 Bio Sidus S A Linhagem celular produtora de eritropoietina humana recombinante e a eritropoietina humana recombinante produzida por esta célula

Also Published As

Publication number Publication date
EP1127063A4 (de) 2005-04-27
WO2000027869A1 (en) 2000-05-18
EP1127063A1 (de) 2001-08-29
CA2704598A1 (en) 2000-05-18
CO5241324A1 (es) 2003-01-31
AU1610000A (en) 2000-05-29
ES2312219T3 (es) 2009-02-16
JP2002529475A (ja) 2002-09-10
US7012130B1 (en) 2006-03-14
JP4516692B2 (ja) 2010-08-04
CA2350327C (en) 2010-05-18
DE69939268D1 (de) 2008-09-18
CA2350327A1 (en) 2000-05-18
BR9917606A (pt) 2002-12-31
EP1127063B1 (de) 2008-08-06

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